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Ming Shun Li,Jong Yul Roh,Xueying Tao,Zi Niu Yu,Zi Duo Liu,Qin Liu,Hong Guang Xu,Hee Jin Shim,Yang-Su Kim,Yong Wang,Jae Young Choi,Yeon Ho Je 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 using PCS which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8-25.2%) with the Rep protein coded by RCR plasmids, however. The putative double-strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. t huringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.
Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma
Tang, Jin-Feng,Yu, Zhong-Hua,Liu, Tie,Lin, Zi-Ying,Wang, Ya-Hong,Yang, La-Wei,He, Hui-Juan,Cao, Jun,Huang, Hai-Li,Liu, Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18
MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR-183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR-93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.
Gong-Yu Hou,Zi-Xiang Li,Kai-Di Wang,Jin-Xin Hu 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.11
Structural deformation monitoring is vital to the safety of concrete structures. However, the distributed deformation of structures cannot be easily obtained using existing monitoring methods in civil engineering. To this end, this paper proposes a method to estimate the continuous deformation of concrete beams by utilizing the distributed optical fiber monitoring technology. In this method, optical fibers and a total station are used to obtain the strain and deformation distribution curves of a concrete beam, respectively. Subsequently, these curves are inputted to a back propagation network as training samples to learn their relationships. The results show that the deformation value of trained neural network is very close to that of the total station, with a maximum error of only 2.7% (0.3 mm). The linear regression analysis shows a goodness of fit R2 greater than 0.98, which confirms the reliability of the simulations results.
감성소재 PET 絲(사)의 계측방법에 따른 열 수축률 변화
김승진 ( Seung Jin Kim ),박경순 ( Kyung Soon Park ),유지수 ( Zi Su Yu ),홍성대 ( Sung Dae Hong ),서봉기 ( Bong Ki Seo ),김연숙 ( Yeon Suk Kim ),심승범 ( Seung Bum Sim ),김소연 ( So Yeon Kim ),박미영 ( Mi Young Park ) 한국감성과학회 2001 추계학술대회 Vol.2001 No.-
본 연구는 국내 7개 회사와 일본과 타이완 기업에서 만들어진 PET POY 사의 습열과 건축수축률을 연구하였다. 이들 사(絲)의 열적수축률들은 hank방법 (KSK 0215)과 Toray FTA-500 장치를 사용하여 측정되었으며 측정된 데이터는 측정방법에 따라 분석되고 토의되어졌다. 준비된 시료는 PET POY 85d/72f와 120d/35f이며 더구나 250d/48f와 155d/50f PET POY가 일본과 국내사 사이의 습열과 건열수축률의 비교를 위해서 역시 준비되어졌다. 그리고 각 시료들의 습열과 건열수축률들은 원사 케이크 내의 사(絲), 50,000m를 1개의 layer로 각각 나누어 각각의 layer 별로 측정되어졌으며 급내와 급간 열수축률이 측정 분석되었으며 국내와 외국 수입사(絲)에 따른 차이를 hank와 Toray 측정방법에 따라 분석하였다.
Yong Liu,Yu-Zi Jin,Chun-He Lee,Jian Huo,이연식 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.2
The reversible addition–fragmentation chain transfer (RAFT) polymerization is one of living radical polymerizations. In this study, four different 9H-fluoren-9-yl benzodithiolates (FBDTs) were synthesized,and used along with azobis(isobutyronitrile) (AIBN), a radical initiator, in polymerization of styrene (ST)at the molar ratio of 3:1. This new transfer agent exhibited the typical characteristic living free radical polymerization behaviors such as good control of molecule weight and narrow molecule weight distribution. It was concluded that the FBDTs can be used as the RAFT agents in free radical polymerization of vinyl monomers.