광합성세균 Rhodopseudomonas palustis 분리 및 IAA와 Carotenoid 생성에 관한 연구

김유경,조영윤,강호준,김정선,양성년,좌창숙,Kim, Yu-Kyoung,Cho, Young-Yun,Kang, Ho-Jun,Kim, Jung-Sun,Yang, Sung-Nyun,Jwa, Chang-sook 한국유기농업학회 2017 韓國有機農業學會誌 Vol.25 No.4

본 연구에서는 제주도 한라산 중산간 습지대 28개소에서 IAA 및 carotenoid 생성능이 우수한 광합성 세균 1종을 최종 선발하였으며, 16S rRNA 염기서열 분석과 생리학적 특성을 조사한 결과 Rhodopseudomonas palustris JK-1 균주로 동정하였다. JK-1 균주의 최적배양조건을 선발하기 위하여 pH, 온도, 빛 및 통기 등을 포함한 여러 가지 요인들이 균주의 생장과 IAA 및 carotenoid 등 광합성색소 생성에 미치는 영향을 조사하였다. 시험결과 JK-1 균주는 명/혐기조건에서 균생장, IAA 및 광합성색소 생성이 양호하였으며 암/혐기조건에서 균생장, IAA 및 광합성색소 형성이 모두 크게 억제되었다. 명/혐기조건에서 균생장, IAA 및 carotenoid 등 광합성색소의 생산을 위한 최적 pH, 온도 및 배양회전속도는 각각 7, $30^{\circ}C$, 150 rpm, 9, $30^{\circ}C$ 및 150 rpm, 그리고 6, $25^{\circ}C$ 및 50 rpm이었다. 그리고 명/호기조건(0.5~1 vvm)에서는 명/혐기조건(0 vvm)보다 균생장 및 IAA 합성이 양호하였으나 광합성색소 형성은 크게 억제되었다. 따라서 최적배양조건은 명/혐기조건에서 pH 7, 온도 $30^{\circ}C$ 및 회전속도 100 rpm을 선발하였으며, IAA 합성을 유도한 배양액을 생육촉진 효과검증에 이용하였다. 시험결과 R. palustris JK-1 균주를 열무에 3% (v/v) 처리 시 지상부 및 지하부 건물중이 무처리 및 배지처리구 대비 각각 20~58% 및 40-28% 증가하였다. The JK-1 isolate which was the best producer of indole-3-acetic acid and carotenoid among the 388 strains isolated from 28 wetlands in Jeju, was identified to be Rhodopseudomonas palustirs belongs to a typical group of non sulfur purple bacteria based on 16S sRNA sequencing. This study investigated the effect of different cultural conditions of pH, temperature, agitation, light and aeration on growth, IAA and carotenoid production of photosynthetic bacterium JK-1 for optimization of IAA and carotenoid production. It was found that growth, IAA, carotenoid, and bacteriochlorophyll production with light (3,000~3,500 Lux) and agitation (100 rpm) showed better results than those with dark/static or dark/agitation (100 rpm) in anaerobic conditions. The optimal pH, temperature and agitation speed for cell growth were 7, $30^{\circ}C$, 150 rpm, for IAA production were 9, $30^{\circ}C$, 150rpm and for carotenoid production were 6, $25^{\circ}C$, 50 rpm, cultured for 72 h under anaerobic light, respectively. The growth and IAA production were high in aerobic culture compared with anaerocic culture, whereas carotenoid and bacteriochlorophyll content were decreased extremely in aerobic condition (0.5~1 vvm). Subsequently, the optimal culture conditions for JK-1 were selected with pH 7, $30^{\circ}C$ and 100 rpm under anaerobic light and the effect on plant growth was tested by pot assay. Inoculation of JK-1 with 3% (v/v) level caused increase in shoot and root dry weigh that varied from 20%~58% to 40%~28% in young radish in camparison to uninoculated treatment at 50 days of growth. The study suggests that the JK-1 isolate may serve as efficient biofertilizer inoculants to promote plant growth.

윤작이 감자 수량, 토양 화학성 및 미생물 활성에 미치는 영향

김유경,강호준,양상호,오한준,이신찬,강성근,김형신,Kim, Yu-Kyoung,Kang, Ho-Jun,Yang, Sang-Ho,Oh, Han-Jun,Lee, Shin-Chan,Kang, Seong-Keun,Kim, Hyoung-Sin 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.4

The objective of this study was to determine crop rotation effects on potato yield, soil chemical and microbiological properties from a short-term field experiment from 2010 to 2011 in Jeju Island, Korea. Potato cropping systems included continuous and rotation sequences of soybean (Glycine max(L.) Merr.), barley (Hordeum vulgare var. hexastichon), rapeseed (Brassica napus L.) and broccoli (Brassica oleracea var. italica). Crop rotations increased the yields of potato from 31% to 52% compared with continuous potato. Marketable yield of potato was highest under soybean plus rapeseed rotation by $20.97MT\;ha^{-1}$ and lowest under continuous cropping by $11.95MT\;ha^{-1}$. The incidence and severity of scab disease was significantly lower in tubers from crop rotation with soybean plus barley. Differences in marketable tuber yields among rotations were associated with potato scab disease. Especially, incidence and severity of potato scab were strongly correlated with soil pH, exchangeable calcium, and bacteria population of the soil. Crop rotations significantly increased soil pH, available phosphate, exchangeable K and Ca, especially in crop rotations with soybean plus barley or rapeseed. Soil microbial biomass C of crop rotations with soybean plus barley or rapeseed, was also significantly higher compared with monoculture. In conclusion, crop rotation may decrease the incidence of soil-born pathogen by increasing soil chemical properties and soil microbial biomass. Overall, potato crop productivity was generally maintained in rotations that contained soybean plus barley or rapeseed but declined under continuous cropping system. 본 시험은 윤작처리가 감자의 상품수량과 토양 화학성 및 미생물 활성에 미치는 영향을 평가하고 알맞은 작부체계를 확립하기 위하여 시험을 수행하였다. 작부체계내 콩과 보리를 도입하여 2년 2기작으로 감자를 윤작하였을 경우 총수량과 상품수량은 대조구인 연작구 대비 각각 31% 및 53% 정도 증가하였으며, 콩과 유채를 도입하여 2년 2기작으로 감자를 윤작하였을 경우 총수량과 상품수량은 각각 57% 및 75% 정도 증가하였다. 그리고 감자의 상품수량에 미치는 요인간 상관관계를 분석한 결과 상품수량은 토양미생물 활성, 특히 Biomass C 및 세균 밀도가 높을수록 증가하였고, 토양 pH, 유효인산, 치환성 칼슘함량이 높을수록 세균 밀도가 증가하였다. 따라서 윤작을 통해서 토양 화학성을 개량하고 미생물 밀도 및 활성을 증가시키면, 토양병 발생을 억제하고 감자의 생산성을 높일 수 있을 것으로 판단된다.

화산회토에서 유기질비료 시용량이 무 생장과 수량 및 양분흡수에 미치는 영향

김유경 ( Kim Yu-kyoung ),조영윤 ( Cho Young-yuen ),오한준 ( Oh Han-jun ),강호준 ( Kang Ho-jun ),양상호 ( Yang Sang-ho ),문봉춘 ( Moon Bong-chun ),좌창숙 ( Jwa Chang-sook ) 한국유기농업학회 2015 韓國有機農業學會誌 Vol.23 No.4

This study was conducted to determine the effects of organic fertilization rates on the nutrient accumulation and recovery in radish (Raphanus sativus L.) as well as growth and yield of radish in Jeju island. An understanding the relationships between organic fertilization rate, crop nutrient recovery and crop yield can assist in making organic fertilizer recommendation which balances crop value and environmental risk in organic cultivation. Nitrogen (T-N), phosphate (P₂O₅) and potassium (K₂O) were applied at 0, 115-35-40, 230-70-80 (standard application rate), 460-140-160, 230-200-100 (recommended application rate) and 158-53-35 kg/ha (customary application rate), respectively as the broadcast application of mixed organic fertilizer (N 4.5% - P₂O₅ 1.5% - K₂O 1%) in combination with langbeinite (K₂O 22%), 100% at sowing period. The organic fertilizer was made of organic materials like oil cakes. Total yield of radish, as fresh weight of roots, increased with increasing organic fertilizer doses to a maximum at rate of standard or soil-testing application. Nitrogen, phosphate and potassium accumulations of radish increased curvilinearly with increasing organic fertilization rate to a maximum at rate of N 460 - P₂O₅ 140 - K₂O 160 kg/ha. However, nitrogen, phosphate and potassium use efficiency of applied organic fertilizer decreased curvilinearly or linearly with increasing organic fertilization rate. Application of organic fertilizer in combination with langbeinite (as a potassium source) had significant effect on the yield of radish. Organic fertilization on a basis of standard or soil-testing application rate is recommended for maximun radish yield in organic cultivation.

항공사 객실승무원의 직무스트레스와 안전행동과의 관계

김유경(Yu-Kyoung Kim) 한국관광연구학회 2016 관광연구저널 Vol.30 No.11

항공사 종사원의 심리적 임파워먼트와 서비스 행동 연구

김유경(Yu Kyoung Kim),김용범(Yong Beom Kim) 한국관광연구학회 2012 관광연구저널 Vol.26 No.6

The object of this study is to examine the relationship between empowerment & service behavior of airline employees using DISC behavioral instrument. Regression analysis & one-way ANOVA test for data analysis were used. A total of 258 airline employees were r&omly chosen in the spring of 2011. The results of the study demonstrated that empowerment has a significant effect on service behavior of airline employees. Specifically, S type using DISC measurement has a significant effect on the relationship between empowerment & service behavior of airline employees. As a result, the present study highlights that empowerment of airline employee can be a critical factor in developing airline service & full underst&ing of personality may be a useful marketing tool in airline industry.

항공사 객실승무원의 직무소진, 지각된 건강상태, 삶의 질, 그리고 몰입과의 관계연구

김유경(Yu Kyoung Kim) 한국관광연구학회 2015 관광연구저널 Vol.29 No.1

Flight attendants interact face-to-face with passengers and face particularly high emotion work demands. The purpose of this study is to investigate the relationship between flight attendant``s job burnout, perceived health, quality of life, and commitment in the summer of 2014 using a sample of 264 Korean flight attendants. The results reveal that burnout of flight attendants have an effect on perceived health and satisfaction which is one construct of quality of life. However, burnout is not an antecedent factor of leisure satisfaction. Moreover, quality of life is a significant effect on commitment in airline industry. These results suggest that airline marketers should consider and understand flight attendant’s emotional burnout and perceived health and provide effective systems for enlargement of flight attendant’s proficiency.

항공서비스전공 대학생의 외모도구성, 외모존중감, 그리고 성형의도간의 관계

김유경(Yu Kyoung Kim) 한국관광연구학회 2013 관광연구저널 Vol.27 No.5

The understanding of appearance has been changing according to the change of social and cultural environment. This study was designed to examine the relationships between appearance instrumentality, appearance esteem, and intention of plastic surgery in undergraduate students majoring in airline service. Regression analysis and one way ANOVA test were used for data analysis. A total of 197 students were randomly chosen in the spring of 2013. The results of the study showed that appearance instrumentality has a significant effect on appearance esteem and intention of plastic surgery. Moreover, appearance esteem is negatively associated with intention of plastic surgery. These results of the present study will be helpful in the understanding of would-be employees` attitude to appearance in terms of human resource management in airline industry.

항공사 객실승무원의 문화차원, 집단성 지각, 내집단 동일시, 서비스행동과의 관계

김유경(Yu Kyoung Kim) 한국관광연구학회 2015 관광연구저널 Vol.29 No.12

The employees’ discrimination against guests has occurred occasionally in the hospitality industry and is affected by culture. Normally, culture have an effect on individual perception, attitude, and behavior. This study sought to assess the significant relationships among the study variables(cultural dimensions, perceived groupness, in-group identification, and service behavior). Three-hundred and fifty flight attendants were randomly chosen in the August of 2014. Of the 350 questionnaires distributed, 307 questionnaires were returned. Due to missing data and incomplete responses, 35 questionnaires were excluded. Consequently, the data for 272 surveys were retained for analysis. The data analysis was conducted with SPSS 15 and AMOS 5. The results were as followed. First, significant antecedents of perceived groupness were revealed to be cultural predictors(Individualism and Masculinity). Second, significant antecedent of in-group identification was perceived groupness and significant consequence of in-group identification was service behavior. In addition, significant antecedents of service behavior were revealed to be Masculinity and Long term orientation of cultural dimensions. Considering the results of the present study, it is recommended that airlines should understand flight attendants’ perception toward cultural dimension and groupness which can affect on service behavior. If airlines consider this suggestion, airlines can enlarge their competitiveness.

공공임대아파트와 분양아파트 유형별 관리비 비교

김유경(Kim Yu Kyeong),지유정(Ji Yu Jeong),박경옥(Park Kyoung Ok) 충북대학교 생활과학연구소 2017 생활과학연구논총 Vol.21 No.1

The purpose of this study is to compare the items of management expense of the public rental apartments and the owned apartments with similar age of construction and similar number of households, and consequently to reveal the cause of big differences of the items. The survey was conducted on the 5 rental apartment complexes and another 5 corresponding owned apartment complexes. Their management expense reports with detailed items of the year 2015 were got from ‘The K-apt System’. Major findings were as follows. (1) The average of total management expense of rental apartment complexes was higher by type than that of the owned apartment complexes. (2) The items making the average higher to the rental apartment complexes were personnel expense, electric charge and general expense. 3. The burden of residents of the rental apartments, however, was not so heavy, according to the management heads of complexes. It was told that the personnel expense might be paid by LH Corporation, the builder, and the electric charge might be paid from the subsidy of local government or supplemented by the extra revenue of complexes.

광합성세균 Rhodopseudomonas palustris 분리 및 IAA와 Carotenoid 생성에 관한 연구

김유경(Yu-Kyoung Kim),강호준(Ho-Jun Kang),김정선(Jeong-Seon Kim),양성년(Seong-Nyeon Yang),좌창숙(Chang-Suk Jwa) 한국토양비료학회 2017 한국토양비료학회 학술발표회 초록집 Vol.2017 No.10