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$17{\alpha}-methyltestosterone$에 의한 능성어 Epinephelus septemfasciatus의 성전환 유도
송영보,백혜자,김형배,이경준,이명돈,Song, Young-Bo,Baek, Hae-Ja,Kim, Hyung-Bae,Lee, Kyeong-Jun,Soyano, Kiyoshi,Lee, Young-Don 한국양식학회 2005 韓國養殖學會誌 Vol.18 No.3
능성어 정자의 안정적 확보를 위하여 MT $0.5{\sim}2.0mg/kg$ BW를 어체 근육내 삽입하여 성전환을 유도하였다 사용한 실험어는 전장 $41.0{\pm}1.3cm$, 체중 $1.4{\pm}0.1kg$이었다. 실험 시작시 능성어 생식소는 주로 gonia cells과 주변인기 단계 난모세포를 가지고 있었다. 실험 종료시 대조구의 생식소는 실험 시작시와 유사하였다. 0.5 mg MT/kg BW 처리구 생식소는 실험 종료 때 소엽내강에 정원세포와 정세포 그리고 정자무리들이 대부분 차지하고 기부에 수정관이 형성되었으나, 일부 어린 난모세포들이 산재하였다. 1.0과 2.0 mg MT/kg BW처리구는 소엽내강과 기부의 수정관에 정자무리들로 가득 차 있었다. 정자는 $1.0{\sim}2.0mg$ MT/kg BW 처리구에서 얻을 수 있었다. MT 처리구의 T혈중농도는 대조구보다 2주 후부터 6주 후까지 높았으나(P<0.05), 배정이 가능한 8주째 대조구와 유사하였다(P>0.05). 11-KT 혈중농도는 MT 1.0처리구는 2주 후부터 6주째까지, 2.0 mg MT/kg BW 처리구는 2주 후부터 실험 종료 때까지 대조구 보다 높았다(P<0.05). $E_2$는 모든 실험어 일부에서 검출되었다. Sex reversal to a functional male of sevenband grouper $(41.0{\pm}1.3cm\;TL,\;1.4{\pm}0.1kg\;BW)$ was induced by $17{\alpha}-methyltestosterone\;(MT,\;0.5\sim2.0mg/kg\;BW)$ implantation from March 17 to May 12,2002. Gonad of control group was composed of genial cells and peri-nucleolus oocyte during the experimental period. Gonad of fish treated with 0.5 mg MT/kg BW had peri-nucleolus oocytes, spermatogonia, spermatids and spermatozoa at the late stages of spermatogenesis, while the fish group treated with 1.0 and 2.0 mg MT/kg BW contained spermatoza in the efferent duct. Sperm were obtained from the experimental groups treated with a dose of $1.0{\sim}2.0mg$ MT/kg BW. In the MT treated groups, testosterone and 11-ketotestosterone levels were higher than those in the control group during the $2{\sim}6$ weeks of the experimental period (P<0.05). $Estradiol-17{\beta}$ was detected from fish in the experimental fish.
Aralia cortex와 Phellodendron cortex의 혼합 추출물이 치주조직세포 활성에 미치는 영향
송영보,이만섭,권영혁,박준봉,허익,김성진,Song, Young-Bo,Lee, Man-Sup,Kwon, Young-Hyuk,Park, Jun-Bong,Herr, Yeek,Kim, Sung-Jin 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.1
The purpose of this study was to evaluate the effect of mixed extracts of aralia cortex and phellodendron cortex (P55A) on activities of human gingival fibroblasts and periodontal ligament cells in vitro. First experiment was done to evaluate the effect of P55A in normal condition. In control group, the cells($4.5{\times}10^4$ cells/ml) were cultured with Dulbecco's Modified Eagle's Medium contained with 10% fetal bovine serum. In experimental groups, P55A was added to the above culture condition at the final concentrations of 0.1 ${\mu}g/ml$(Test group 1), 1 ${\mu}g/ml$(Test group 2) and 10 ${\mu}g/ml$(Test group 3). Then each group was tested for the cell proliferation rate at $\frac{1}{2}$, 2, 5 days, protein levels at 2, 5 days, and alkaline phosphatase activity at 2, 5 days. Second experiment was done to evaluate the effect of P55A in high glucose condition. 200 mg/dl glucose was added to the same culture condition of all groups in first experiment. Then each group was tested for the cell proliferation rate at $\frac{1}{2}$ , 2, 5 days, protein levels at 2, 5 days, and alkaline phoaphatase activity at 2, 5 days. The results were as follows ; 1. First experiment 1) As P55A concentration increased, cell proliferation rate increased significantly in test group 2 at 2 days, and test group 2 and 3 at 5 days in human gingival fibroblasts and periodontal ligament cells(P<0.05). 2) In human gingival fibroblasts, all test groups showed significantly increased protein levels as compared to control group at 5 days. In periodontal ligament cells, test group 2 and 3 showed significantly increased protein levels as compared to control group at 2, 5 days(P<0.05). 3) Alkaline phosphatase activity of human periodontal ligament cells increased as P55A concentration increased. The test group 2 and 3 showed significant increase as compared to control group at 5 days(P<0.05). 2. Second experiment 1) As P55A concentration increased, cell proliferation rate increased significantly in test group 2 at 2 days, and test group 2 and 3 at 5 days in human gingival fibroblasts and periodontal ligament cells(P<0.05). 2) In human gingival fibroblasts, test group 3 showed significantly increased protein levels as compared to control group at 2 days, and all test groups at 5 days. In periodontal ligament cells, test group 2 and 3 showed significantly increased protein levels as compared to control group at 2, 5 days(P<0.05). 3) Alkaline phosphatase activity of human periodontal ligament cells increased as P55A concentration increased. The test group 2 and 3 showed significant increase as compared to control group at 2 days, and all test groups at 5 days(P<0.05). From the above results, mixed extracts of aralia cortex and phellodendron cortex appeared to enhance cellular activities including cell proliferation rate, protein levels and alkaline phosphatase activity of human gingival fibroblasts and periodontal ligament cells in normal and high glucose condition. This study suggests that mixed extracts of aralia cortex and phellodendron cortex seem to be able to subside the inflammation of periodontal tissue and regenerate the destructed periodontal tissue.
홍바리, Epinephelus fasciatus 정자의 미세구조
김성훈,이치훈,송영보,주해성,김형배,이영돈,Kim, Seong-Hoon,Lee, Chi-Hoon,Song, Young-Bo,Ju, Hea-Sung,Kim, Hyung-Bae,Lee, Young-Don 한국현미경학회 2012 Applied microscopy Vol.42 No.2
Ultrastructural characteristics of blacktip grouper, Epinephelus fasciatus spermatozoa were investigated using transmission and scanning electron microscopy. The spermatozoa of E. fasciatus consisted of a spherical head part, a midpiece with cytoplasmic canal entrance and a flagellum with lateral fins. Internal ultrastructurally, the nucleus contains high electron dense chromatin having granular particles and has no acrosome. The centriolar complex lies outside of the nuclear fossa and it is connected by the osmophilic filaments. Also the osmophilic filaments connect between the centriolar complex and the nuclear membrane. The midpiece contains eight to nine spherical mitochondria, cytoplasmic canal and necklaces. The flagellum has a typical 9+2 axonemal structure. The lateral fins contain vesicles and a typical 9+2 axonemal structure. Consequently this study contributes to comparative grouper spermatology and provide useful systematic taxonomic characters. 홍바리, Epinephelus fasciatus 정자의 미세구조적 특징을 주사형 및 투과형 전자현미경으로 관찰하였다. 홍바리 정자는 구형의 두부와 cytoplasmic sleeve와 고리형 cytoplasmic canal 입구 등으로 구성된 중편부 그리고 lateral fin을 갖는 1개의 편모로 구성된 미부로 구별된다. 정자의 핵은 구형이며, 첨체 구조물은 존재하지 않으나 이질 염색질이 과립상 덩어리 형태로 조밀하게 분포하였다. Proximal centriole과 distal centriole은 각각 수레바퀴형의 "9+0"의 축사구조를 보이며 서로 수직으로 osmophilic filaments 연결되어 있고, 핵막과도 연결되어 있었다. 중편부는 구형의 8~9개의 미토콘드리아를 포함하며, 빈공간인 cytoplasmic canal과 편모 원형질막에는 7~8개의 구형 입자를 갖는 necklace가 존재하였다. 미부는 1개의 편모를 갖으며 전형적인 "9+2"의 축사구조를 보였고, 편모에는 다양한 형태의 lateral fins을 관찰할 수 있었다. 홍바리 정자의 미세구조적 특징은 바리과 어류의 정자와 상이하여 계통분류학적 동정의 형질이 될 수 있을 뿐만아니라 정자 세포소기관의 기능적 및 생리학적 연구에 이용될 수 있다고 판단된다.
송영보,이치훈,나오수,이영돈 濟州大學校 海洋硏究所 2002 해양과환경연구소 연구논문집 Vol.26 No.-
1995년 9월부터 1996년 8월까지 제주대학교 해양과 환경연구소가 위치한 함덕연안 조간대에서 채집한 울타리고둥, M. labio의 생식주기를 조사하기 위하여 생식소숙도지수의 변화와 생식세포 형성과정을 조직학적방법으로 조사하였다. 1. 울타리고둥의 생식소는 패각내 나선상 육질부 하단에서 꼬리돌기까지 간장부의 표면에 위치 하였다. 생식소가 성숙하면 암컷은 짙은 녹색, 수컷은 유백색을 띠고, 방출 후에는 암컷은 연갈색, 수컷은 연황색을 나타내었다. 2. 생식소숙도지수(GSI)는 암컷과 수컷 모두 수온이 상승하는 4월부터 상승하기 시작하여 9월에 암컷 0.47, 수컷 0.42로 최고치를 보이고 이후 10월부터 급격히 감소하였다. 저수온기인 1월에 GSI는 암컷과 수컷 모두 0.07로 최저치를 보였다. 3. 생식소의 발달 단계는 분열증식기(3월∼4월), 성장기(4월∼7월), 성숙기(8월∼9월) 그리고 방출 및 회복기(10월∼1월)의 연속적인 주기로 구분 할 수 있고, 주산란 시기는 10월로 추정 된다. 4. 울타리고둥, M. labio은 자웅이체로서, 성비는 약 1:1이었다(P>0.05). Reproductive cycle of thick liped monodont. Mondonta labio was invetigated by the histological observation of gonads and the gonadosomatic index(GSI). The thick liped monodont were collected at the intertidal zone of Hamdeok in Jeju-do from September, 1995 to August, 1996. Gonad of them was located o the surface of the liver below the stomachal caecum posterior spiral meat part of the shell. GSI value began to increase from May(as water temperature increased) and reached it's maximum value in September both male and female which were 0.42 and 0.47, respectively. It began to decrease from October thereafter, maintaining relatively in low value from January to March. The reproductive cycle of this species could be classified into four successive developmental stage; multiplication stage(March to April), growing stage(April to July), mature stage(August to september), spent and recovery stage(October to January). The main spawning period of M. labio appeared in October. The top shell, M. labio appeared to be gonochorism, neither sex reversal nor hermaphroditism. The sex ratio was approximately 1.0 : 1.0(P>0.05).