사과 왜성대목 M.9 및 M.26의 고온, ribavirin, 생장점 배양을 통한 바이러스 제거

권영희,이정관,김희규,김경옥,박재성,허윤선,박의광,윤여중,Kwon, Young Hee,Lee, Joung Kwan,Kim, Hee Kyu,Kim, Kyung Ok,Park, Jae Seong,Huh, Yoon Sun,Park, Eui Kwang,Yoon, Yeo Joong 한국식물생명공학회 2019 식물생명공학회지 Vol.46 No.3

사과(Malus pumila)는 국내에서 가장 경제적으로 중요한 과수 중의 하나이다. 하지만 사과 바이러스 감염은 생산량을 감소시키고 수확량 손실과 과일 품질 저하와 같은 심각한 문제를 야기한다. 국내에 감염된 사과 바이러스 및 비로이드 종류는 Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV)와 Apple scar skin viroid (ASSVd) 등이 알려져 있다. 사과는 바이러스나 비로이드에 감염되어 있어도 대체로 이상한 징후가 발견되지 않아 바이러스로 인해 피해가 많았다. 본 연구는 사과 왜성대목 M.9 및 M.26의 무독묘 생산을 위하여 고온처리($37^{\circ}C$, 6주), 화학처리(Ribavirin) 및 생장점 배양하여 바이러스 제거 처리를 하였다. 바이러스 검출에 일반적으로 사용되는 방법은 효소면역 측정법(ELlSA)과 중합효소연쇄반응(RT-PCR)을 이용하였는데, RT-PCR은 ELlSA방법보다 10 ~ 30% 더 민감하였다. 사과 왜성대목 바이러스 검정 결과, 바이러스 제거 효율은 생장점 배양이 가장 높았다. 생장점 배양 후 바이러스 무병묘의 획득율은 30 ~ 40%로 높게 나타났다. 생장점 배양에서 사과 왜성대목 M.9은 ACLSV, ASPV 및 ASGV의 비율이 각각 45%, 60%, 50%로 높았고, 사과 왜성대목 M.26에서는 ACLSV, ASPV 및 ASGV의 감염율은 각각 40%, 55%, 55%였다. 이상의 결과, 사과 왜성대목에서 무독묘를 생산할 수 있는 가장 효과적인 방법은 생장점 배양에 의한 것으로 판단되었다. Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy ($37^{\circ}C$, 6 weeks) and chemotherapy($Ribavirin^{(R)}$). The detection of apple viruses was accomplished by Enzyme-linked Immuno-Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR method was 10 ~ 30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30 ~ 40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.

전통누룩 진균류를 이용한 입국의 제조 및 입국곰팡이의 동정

김재호 ( Jae Ho Kim ),권영희 ( Young Hee Kwon ),이애란 ( Ae Ran Lee ),김혜련 ( Hye Ryun Kim ),안병학 ( Byung Hak Ahn ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.4

Various koji were prepared by fungi isolated from traditional nuruk and their quality characteristics were investigated. Acidity and saccharification power of their koji were ranged in 5.0~6.8 and 128sp~241sp. Nine fungi which were showed good quality and sensory evaluation were identified by analysis of their nucleotide sequences with PCR-amplified 18S rDNA internal transcribed spacer-1(ITS-1) and ITS-4 genes. Among them, six strains were identified as Aspergillus oryzae and the other strains were identified as Mycocladus corymbiferus, Rhizopus oryzae, Lichtheimia corymbifera.

새로운 모더니즘 연구들 -모더니즘의 제국주의 문맥을 중심으로-

권영희 ( Young Hee Kwon ) 영미문학연구회 2009 안과 밖 Vol.0 No.27

대처시대 여성 동성애와 이민자 서사 겹쳐 읽기 -『오렌지 말고 다른 과일도 있어』와 『속하지 못한 사람들』-

권영희 ( Young Hee Kwon ) 영미문학연구회 2009 안과 밖 Vol.0 No.26

쌀 품종을 달리한 입국의 제조 및 막걸리의 품질 특성

권영희(Young-Hee Kwon),이애란(Ae-Ran Lee),김혜련(Hye-Ryun Kim),김재호(Jae-Ho Kim),안병학(Byung-Hak Ahn) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.1

본 연구에서는 쌀 품종을 달리하여 제조한 입국을 사용하여 각각의 쌀 품종 별로 탁주를 제조하였다. 19품종의 쌀로 제조한 입국은 쌀 품종에 관계없이 산도 5.0 이상, 당화력 60 이상의 입국기준의 조건을 충족하였다. 입국을 사용하여 담금 한 막걸리의 알코올 함량은 11.5-14.5%(v/v)로 나타났으며 발효가 진행되는 동안의 pH와 총산 고형분함량은 입국 제조에 사용된 쌀의 종류에 따라 각각 다른 양상을 보였다. 알코올 함량을 6%(v/v)로 낮추어 분석한 결과 pH는 3.47-3.76로 시판막걸리의 수준을 나타내었으며 총산은 0.27-0.44%였다. 고형분 함량과 환원당 함량은 각각 2.7-4.6° Bx와 2.49-6.01 mg/mL를 나타내었다. 유기산과 유리당의 분석 결과 유기산은 5종(oxalic, malic, lactic, acetic, succinic acid)과 함께 입국으로부터 기인한 citric acid가 검출되었다. 유리당은 glucose, maltose, fructose가 검출되었으며 그 중 glucose의 함량이 가장 높게 나타났다. 관능검사 결과 외관의 기호도는 차이를 보이지 않았으며 화영, 호품 그리고 수라 품종을 사용한 막걸리의 기호도가 높은 것으로 나타났다. The aim of this research was to analyze makgeolli with nineteen kinds of rice and koji. Among 19 rice, 18 rice were domestic products and one rice was an import. For the properties of koji made with 19 kinds of rice, the acidity was over 5.0 and the saccharogenic power was more than 60SP. Makgeolli brewed with 19 kinds of rice and the koji of each rice were analyzed for alcohol contents, pH, total acidity and solid contents during fermentation. After fermentation, raw liquor’s alcohol degree was set 6.0%, the pH ranged 3.47-3.76, the total acidity 0.27-0.44%, the solid contents and the reducing sugar were 2.7-4.6oBx and 2.49-6.01 mg/mL. Organic acid was detected 5 kinds (oxalic, malic, lactic, acetic, succinic acid) and free sugars were found such as glucose, maltose and fructose. Hwayoung, Hopum and Sura were higher than the residue in 15 kinds of rice in the preference test.

SNMP에이전트 소프트웨어의 재사용을 위한 구조 설계

권영희 ( Young Hee Kwon ),김영집 ( Young Jip Kim ),박애순 ( Ae Soon Park ),조은경 ( Eun Kyoung Cho ),구연설 ( Yeon Seol Koo ) 한국정보처리학회 2000 정보처리학회논문지 Vol.7 No.6

With the wide usage of the Internet, there use of pre-developed software is becoming important more and more in the development of various communication software. In this paper, the architecture to reuse the communication software is proposed. It is applied to the development of MIB-II system group of SNMP agent software for reusing. If Communication software is developed using this architecture, the software development life cycle will be abridged and the development cost will be reduced.

클래스 노드 분석에 의한 객체 지향 소프트웨어 회귀 테스팅

권영희(Young Hee Kwon),이인혁(Jen Ge Li),구연설(Yeon Seol Koo) 한국정보처리학회 1999 정보처리학회논문지 Vol.6 No.12

In this paper, we propose an improve regression testing method, which use method as the basic unit of changing. The test method consists of three steps. We represent the relationship of classes using the notation of UML(Unified Modeling Language), find the nodes of the modified and affected methods by node analysis, and then select changed test cases from the original test cases. The proposed object-oriented regression testing method can reduce the number of test cases, testing time and through reuse of test cases.

배추무름병 원인균 분리 및 특성 연구

권영희(Young Hee Kwon),유아영(Ah Young Yoo),유종언(Jong Earn Yu),강호영(Ho Young Kang) 한국생명과학회 2009 생명과학회지 Vol.19 No.8

배추 무름병을 대상으로 식물병원균 감염모델을 확립하기 위하여 무름병변을 가진 배추조직으로부터 80개의 독립된 세균집락을 순수 분리하였다. 이들 균을 감염되지 않은 배추 잎의 주맥부위에 접종하여 24~48 시간 만에 무름병변을 나타내는 8개의 균주를 1차 선별하였다. 다양한 미생물학적, 생화학적, 형태학적인 시험을 통하여 분석한 결과 서로 다른 특성을 나타내는 균이라고 여겨지는 3개의 균을 최종 선정하였고 이들은 모두 그람 음성균으로 판명되었고 RB1, RB2 및 RB6로 명명하였다. API 20E시험, VITEK 2 COMPACT 분석, 16S rRNA 염기서열 분석 등을 종합할 때 RB1 및 RB2는 Erwinia carotovorum subsp. odoriferum 아종으로, RB6는 Erwinia carotovorum subsp. carotovorum 아종으로 추정되었다. 이들 균들은 30℃, 생리적 pH인 중성 pH에서 최적생육을 하였다. 이들은 배추의 상처 유무에 상관없이 초기 접종양과 비례하여 무름병을 유발하였으며, 상처가 없는 경우보다 상처가 있는 경우에 더욱 명확한 병변을 유발하였다. RB1의 경우 8.0×10? CFU/㎖, RB2 균주는 10? CFU/㎖, RB6는 4.7×10? CFU/㎖에서 최초로 무름 증상을 나타냈다. 이들 균의 숙주 특이성을 관찰하기 위하여 14종의 야채에 접종한 결과 배추, 가지 파프리카에서만 무름병변을 유발하는 숙주 특이성을 나타내었다. 이 연구에서 사용된 실험재료들 및 감염모델은 향후 식물병원균의 감염기작해석에 크게 기여할 것으로 예상된다. In order to establish in vitro infection model for research of plant pathogen based on tissue softening disease in napa cabbage, eighty independent bacterial strains were isolated from the softened napa cabbage tissues. Eight bacterial isolates were primarily screened with the generation of reproducible tissue softening disease to fresh napa cabbages within 24~48 hours after inoculation. Through various microbiological, biochemical and morphological examinations, three Gram (-) isolates which harbor independent biological properties were finally chosen, and named as RB1, RB2 and RB6. Collective results obtained from API 20E test and analyses of VITEK 2 COMPACT and nucleotide sequences of 16S rRNA of each isolate proposed that isolates RB1 and RB2 are close to the Erwinia carotovora subsp. odorifera, and RB6 is close to the Erwinia carotovora subsp. carotovora. These isolates grew optimally at 30℃ with neutral pH culture condition. The isolates caused softening tissue disease with dose-dependent manner regardless of pre-surface damages of napa cabbage. Minimum dose to cause soft rot disease for RB1, RB2 or RB6 were 8.0×10? CFU/㎖, 10? CFU/㎖ or 4.7×10? CFU/㎖, respectively. These isolates caused tissue softening disease to eggplant, paprika and napa cabbage out of 14 different tested vegetables, indicating that these isolates damages specific plant tissues. The bacterial isolates obtained in this research and in vitro plant infection model will be adapted in the understanding of the mechanism of pathogenesis by plant pathogen.

발효, 세포배양, 생물공정 찹쌀의 종류와 전처리를 달리한 약주의 특성 및 휘발성 향기 성분

권영희 ( Young Hee Kwon ),조성진 ( Sung Jin Jo ),김재호 ( Jae Ho Kim ),안병학 ( Byung Hak Ahn ) 한국미생물생명공학회 2010 한국미생물·생명공학회지 Vol.38 No.1

특권의 울타리 밖으로: 『어둠이 오기 전 여름』에 나타난 여성 주체성과 자유의 문제

권영희 ( Young Hee Kwon ) 영미문학연구회 2015 영미문학연구 Vol.29 No.-

Doris Lessing’s The Summer Before the Dark prefigures the third-wave feminism’s critique of the unitary notion of female subject hood that undermined earlier women’s liberation movements. Centered on Kate Brown’s evolving consciousness, the novel illumines her spiritual journey to the recognition that while she has been sacrificed as an angel-in-the-house, she nevertheless is a beneficiary in the global system of exploitation. Lessing, in the earlier half of the novel, posits the protagonist along the two familiar lines of women’s liberation narratives: career pursuit and sexual autonomy. It becomes clear, though, neither work nor sex will salvage Kate from her deep sense of failure and intense spiritual crisis. Instead, Lessing allows her to experience a series of mirroring encounter; people of diverse social status induce her to reflect on what she really is in a way that opens her eyes to crucial facets of socioeconomic formation and operation, illuminating how they have determined her seemingly unique personality, not to mention of her life devoted to her family. Further, Kate learns to symbolically align herself with the underprivileged through a per formative act of living as an ignored and therefore ‘invisible’ woman owing to unattractive look. Lessing ends this novel without presenting how Kate’s experience may result in significant changes in her life. Nevertheless, in the final act of returning home is embedded a potential of alternative forms of female subjectivity and liberation, rather than a debilitating failure or a gloomy social vision.