經濟作物 改良을 위한 遺傳工學 技術의 開發 : I. Nicotiana PlumBaginifolia의 葉肉細胞에서 原形質體 裸出과 再分化 I. Protoplasts isolation from leaf mesophyII cells of Nicotiana plumbaginifolia and regeneration

蔡永岩,崔奎煥 서울大學校 農科大學 1985 서울대농학연구지 Vol.10 No.1

Various innovations for the genetic modification of plants are being investigated in aiming to produce new varieties that can not be made by the conventional plant breeding scheme. One of the most promising approach in plant transformation is to introduce new genes into plant protoplasts and regenerate these protoplasts into whole plants with new improved characteristics. Before embark directly on a target economic crop, however, it seems a right way to establish firstly the feasibility of the protoplast approach in a model plant species where the cycle of plant to protoplast to plant is well established. When the protoplast approach was worked beautifully on the model species, then this technique would be extended to a economic crop with its difficulties in plant regeneration from the protoplasts. This report describe the procedures established through a serious of experiments for the optimum growth and regeneration conditions of the protoplasts isolated from Nicotiana plumbaginifolia leaf mesophyll cells.

지황에서 신초의 유도와 신장 및 순화

채영암 서울대학교 농업개발연구소 2000 농업생명과학연구 Vol.4 No.-

This experiment was carried out to find the proper way for shoot induction and elongation in bioreactor culture of Rehmannia glutinosa and acclimatization for field cultivation. Multiplication rate of the explants was significantly improved in the induction media with 1.0mg/L thidiazuron alone. Multiple shoots induced in media(MS media with thidiazuron 1.0mg/L) supplemented with paclobutrazol 0.3mg/L had significantly lower vitrification rate. The presence of paclobutrazol during shoot induction decreased the number of shoots and nodes per shoot. Half strength MS media with 1.2% agar and 3% sucrose improved acclimatization and rooting of shoots derived from bioreactor culture. Shoots were effectively acclimatized and rooted well in vessel with membrane filter. Shoots acclimatized in vessel with membrane filter were healthier and had higher ex vitro survival rate than those without membrane filter in plug tray.

전통 향료 유전자원 산초 및 초피의 화학형 분류

채영암 서울대학교 농업개발연구소 2001 농업생명과학연구 Vol.5 No.-

Z. schinifolium and Z. piperitum have been used as natural favorite spice in Korea and Japan. Major volatile components in leaves of Z. schinifolium were α-pinene, cis-ocimene and trans-β-ocimene which comprized of 40% in total essential oil and α-pinene, limonene+β-phellandrene+1,8-cineole which of 64% in Z. piperitum. Volatile composition was varied with growth stages. Four chemotypes were identified based on the component of essential oil both in Z. schinifolium and Z. piperitum. Estragole was appeared in the fruit of Z. schinifolium, which increased further as ripening of fruits while azulene was detected in the fruit of Z. piperitum.

담배속에서 原形質體 融合과 植物體 再分化

崔奎煥,蔡永岩 서울大學校 農科大學 1988 서울대농학연구지 Vol.13 No.1

This experiment was carried out to determine the proper condition for protoplast isolation and fusion in Nicotiana sylvestris and N. plumbaginifolia, and plant regeneration from fused cells. Enzyme mix of 2.0% cellulase R10 0.5% macerozyme R10 was proper for protoplast isolation from leaf mesophyll cells. Optimum fusin frequency was observed in solution(pH5.8) of PEG(6000) 35% and 9mM CaCl₂Callus was induced 20 days after plating on culture medium. Shoots were formed 6 weeks after plating on shooting medium and rooted on MS basal medium thereafter. Three out of 20 regenerated plants were identfied as somatic hybrids by peroxidase isozyme analysis.

청피홍심무 현탁배양에서 세포생장과 색소생산에 미치는 요인

최규환,채영암 서울大學校 農業生命科學大學 農業開發硏究所 1996 서울대농학연구지 Vol.21 No.1

음주운전 처벌 강화법 실시전후에 따른 교통사고 발생양상변화

안정태,이동배,조영채,박암 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.1

In order to compare the characteristics of traffic accident trends before and after the amendment of The Road Traffic Law, the author analyzed 541 accident victims for one month before and after the amendment of The Road Traffic Law. The findings of study were summerized as follow: 1. Traffic accidents were decreased 36.2% after amendment of The Road Traffic Law than before, which were decreased highly in the low education group and the drive career over ten years group. 2. Traffic accidents were most frequent during the time 12:00-20:00. For the comparison between before and after amendment of The Road Traffic Law, during the time between 20:00-24:00, the traffic accident was decreased with 54.3%, but during the time between 24:00-04:00, the traffic accident was increased with 70%. 3. Traffic accidents by the motorcar was most frequent, but the rate by bus and microbus was highly decreased after the amendment of traffic law. For the cause of accidents, unobeyed safety driving was most frequent cause, but that was decreased conspicuously among alcohol drinking driving and over speed driving. 4. Most patients were injured mildly, and traffic accident rate of it was far lower than injured severely or death after the amendment of the traffic law. Most traffic accidents were sprain and contusion. Fractures were decreased the highest rate after the amendment of the Road Traffic Law. The most patients was treated for 2 weeks, but treated over 4 weeks was decreased least. 5. For the state of traffic accidents, severe injuries, head traumas and fractures were higher rate among drinking driving, but mild injuries, sprains and contusions were higher rate among nondrinking driving. The duration of treatment was short for the accidents by non-drinking drive, but that was long among by the drinking drive.

In vitro breeding for salt-tolerance rice Ⅲ. Detection of reliable selection criterion for salt-tolerance evaluation

Young Am Chae(蔡永岩),Yong Ho Kim(金龍昊),Jung Gee Heu(許貞基) 한국육종학회 1989 한국육종학회지 Vol.21 No.1

Proline, Na, K and Ca ion contents in the callus cells cultured on the medium with and without NaCl were analyzed to determine reliable selection marker for salt-tolerance evaluation and later in quantitative genetic analysis of salt-tolerance. Even though there was no statistically significant varietal difference in proline content of the callus grown on salt-free medium, proline accumulation ability of the callus was significantly different under salt-stress while the cation contents in the callus grown both on the media with and without NaCl was significantly different. The rank of proline content in the 10 different varietal callus cultured on salt-free medium was highly coincided with the rank of proline accumulation under saline stress but three was no such relationship in case of the cation contents. Larger variability in proline accumulation even under NaCl-stress could provide easier identfication of the salt-tolerant callus. The range of variation in the cation contents was much narrowed under saline condition. Thus measure of proline accumulation in callus may be the most reliable selection criterion for salt-tolerance evaluation.

In vitro breeding for salt-tolerance rice Ⅳ. Genetic analysis for proline content in progeny of F₂generation in relation to salt-tolerance

Young Am Chae(蔡永岩),Kwan Ho Bang(方寬虎),Jeung Kee Heu(許貞基) 한국육종학회 1989 한국육종학회지 Vol.21 No.4

Proline content in the F₂ seed-derived callus grown on salt medium was analyzed to know the mode of segregation and genetic analysis in the F₂ generations. Linkage analysis implied that at least six linkage groups were involved in proline accumulation related to salt-tolerance. The segregation of proline accumulation in the progeny of Gihobyo/Annapurna F₂ generation was bimodal. The well-fitted segregation ratio of 15:1(low:high) indicated that two genes were involved and they were complementary. Proline content in the progeny of Annapurna/Nagdongbyo F₂ generation showed normal distribution, which suggested many genes might be concerned in this cross combination.

밀의 초장결정 유전자분석

Chae Young-am 韓國作物學會 1982 Korean journal of crop science Vol.27 No.3

밀의 초장을 결정하는 유전자의 소재를 밝히기 위하여 21개 Caribo monosomic lines에 재배종인 Diplomat을 교배하여 얻은 F1 식물체를 근 선단부의 염색체를 검경하여 monosomic F1 을 확인한 다음 자식시켜 F2 집단을 얻었다. 초장을 결정하는 유전자를 밝히기 위하여 monosomic F2 평균과 정상교배 F2 평균을 비교한 결과 적어도 7개의 유전자가 초장에 관여하고 있었다. 이중 2A, 2B, 2D는 초장을 작게하는 주인자로, 그리고 5A는 초장을 크게 하는 주인자로 나타났다. 또한 3B, 6A, 7A도 초장을 작게하는 인자이다. 2A, 2B, 2D 및 5A와는 달리 그 효과가 작은 것으로 분석 또는 해석되었다. F2 progenies from the crosses between 'Caribo' monosomic lines and 'Diplomat' revealed significant differences in plant height in 7 F2 populations. Well known effects of monosomic constritution of group 2 homoeologues and chromosome 5A sufficiently well explain reduced or increased plant height in these crosses respectively. Minor gene effects were recognized from 'Diplomat' chromosomes 3B, 6A and 7A and interpreted to explain or to contribute to genetic control of the rather small differences in plant height between cv. 'Caribo' and 'Diplomat'

감자와 가지의 原形質體 融合

Young am Chae(蔡永岩),Kyu whan Choi(崔奎煥) 한국육종학회 1986 한국육종학회지 Vol.18 No.4

This study was carried out to find out the proper enzyme mix for protoplast isolation, and the effects of PEG, pH and Ca ion on the fusion. Mix of 2% cellulase R10 with 0.5% macerozyme R10 and 2.5% cellulase with 0.75% macerozyme were proper for isolation of protoplasts from mesophyll tissues of potato and egg plant, respectively. Fusion frequency was higher in each of 35% PEG(MW 6000), 15 mM Ca ion and at pH 5.5, however, their combined effect was not greater than as it was expected.