Characterization of Matrix Metalloproteinase Expression in Triglyceride Treated THP-1 Macrophages

Yoonjung Cho,Jaewon Lim,Dong Hyun Lee,Byung Chul Jung,Min Ho Lee,Dongju Jung,Yoon Suk Kim,Tae Ue Kim,Ki-Jong Rhee 대한의생명과학회 2013 Biomedical Science Letters Vol.19 No.1

Elevated blood triglyceride (TG) levels correlate with development of atherosclerosis suggesting that TG may promote the development of this disease. During atherosclerosis, TG is taken up by tissue macrophages which result in dramatic changes in various secreted factors. One such factor is the family of matrix metalloproteases (MMP) which are involved in tissue remodeling during both physiological and pathological processes. In this study, we examined the MMP expression profile in PMA-differentiated THP-1 macrophages treated with TG. We found that TG-treated THP-1 macrophages showed decreased expression of MMP-3, MMP-7, MMP-8 and MMP-9 in a time- and dose-dependent manner. In contrast, expression of MMP-1, MMP-2, and MMP-10 remained relatively unchanged after TG treatment. In addition, we found that expression of select MMPs was affected by various inhibitors of signaling pathways. In particular, expression of MMP-3 was slightly recovered by cRAF and PLC signaling pathway inhibitors. These data suggests a possible role of MMPs in macrophages during TG-induced atherosclerosis.

Validation of Reduced Reagent Volumes in the Implementation of the Quantifiler^® Trio Quantification Kit

Cho, Yoonjung,Kim, Hyo Sook,Kim, Min-Hee,Park, Moonhee,Kwon, Hansol,Lee, Yang Han,Lee, Dong Sub Wiley (Blackwell Publishing) 2018 Journal of forensic sciences Vol.63 No.2

교사동기 연구의 동향과 과제

조윤정(Cho, YoonJung) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.5

교사동기는 교사의 교직업무 뿐만 아니라 학생의 학습에 광범위하게 영향을 미친다는 점에서 매우 중요한 연구주제임에도 불구하고, 학업동기에 비해 연구자들의 관심을 받은 지 얼마 되지 않았다. 본 연구의 목적은 교사동기를 살펴본 선행연구들의 연구동향을 고찰함으로써 교사동기 연구의 현주소를 이해하고 향후 연구의 방향을 제시하는 데 있다. 그동안 수행된 교사동기 연구들은 교직의 특수성을 반영한 동기이론을 개발하여 적용하기 보다는, 학생을 대상으로 한 기존 연구에 사용되었던 동기이론을 교사동기에 단순히 적용한 경우가 대부분이었다. 본 연구에서는 교사동기 연구에 적용된 주요 동기이론들(자기효능감, 성취목표이론, 자기결정성이론, 기대-가치이론, 성취정서이론)을 검토하고, 이러한 이론에 근거하여 도출된 주요 교사동기 구인들(교사효능감, 성취목표, 자율성동기, 교직선택동기, 성취정서)을 중심으로 연구동향의 주요 특징과 패턴을 분석하였다. 향후 교사동기 연구에서 주목해야할 과제로 교사동기 특수성에 부합하는 동기이론 개발 및 적용, 교사동기의 종단적 변화와 안정성, 교사가 ‘지각한’ 동기의 특성과 선행요인, 교사동기와 관련 변인들 간의 인과적 관계, 교사동기 측정방식의 다각화를 제안하였다. 본 연구는 교사동기에 대한 학문적 관심이 급격히 증가하는 시점에서 교사동기 연구자들이 주목해온 주제들과 연구결과들을 살펴보고, 중요성에 비해 소홀히 다루어진 연구주제들을 발굴하여 향후 과제로 제안했다는 점에서 학문적 의의를 찾을 수 있다. Despite the significant influence of teacher motivation on student learning and instructional practices, teacher motivational research is still in its infancy. It is critical to understand teacher motivation in terms of its unique characteristics, antecedents and consequences to effectively promote adaptive teacher motivation. Early research on teacher motivation focused on examining whether the motivational theories utilized in student motivational research are applicable to explain teacher motivation. The purpose of this study is to understand the current status of teacher motivation research and to suggest directions for future research. This study focused on reviewing teacher motivation studies that applied the main theories of motivation (self-efficacy, achievement goal, self-determination, expectancy-value, achievement emotion theories) as theoretical framework and examined the main characteristics and patterns of research trend in the field of teacher motivation. In addition, this study suggested the direction and prospect of future research on teacher motivation. It was discussed that researchers need to pay attention to revising and expanding the motivation theories suitable for the research on teacher motivation in order to reflect unique and distinctive aspects of teacher motivation. This study also suggested that domain-specific and situation-specific nature of teacher motivation need to be captured and that causal relationships between teacher motivation and related variables need to be investigated. As research interest in teacher motivation is rapidly increasing, it is meaningful to identify the important research topics of teacher motivation that have been relatively neglected.

교사가 지각한 학교목표구조와 성취목표의 관계에서 내재동기의 조절효과

조윤정 ( Cho Yoonjung ),황소리 ( Hwang Sori ),심성민 ( Sim Sungmin ),강스텔라 ( Kang Stella ) 안암교육학회 2021 한국교육학연구 Vol.27 No.4

본 연구에서는 교사가 지각한 학교목표구조가 교사의 성취목표에 미치는 영향이 교사의 내재동기에 따라 달라질 것으로 예측하고, 교사의 성취목표를 예측하는 학교목표구조(환경변인)와 내재동기(개인변인)의 독립적 효과뿐만 아니라 변인 간 상호작용 효과를 살펴보았다. 총 246명의 교사들이 성취목표(숙달, 수행접근), 학교목표구조(숙달, 수행), 그리고 내재동기 문항에 응답하였다. 중다회귀분석 결과, 학교숙달목표구조는 교사의 숙달목표를, 학교수행목표구조는 교사의 수행접근목표를 각각 정적으로 예측하였고, 내재동기는 교사의 숙달목표를 정적으로 예측하였다. 교사가 지각한 학교목표구조와 내재동기 간의 상호작효 효과를 살펴본 결과, 교사의 내재동기가 학교숙달목표구조와 교사의 숙달목표, 학교숙달목표구조와 교사의 수행접근목표 간 관계를 조절하는 것으로 나타났다. 본 연구 결과는 교사가 지각한 학교의 목표구조가 모든 교사에게 동일한 방식으로 영향을 미치기보다는 교사 개인의 내재동기에 따라 더 강력한 영향을 행사할 수도 있고 그렇지 않을 수도 있음을 시사한다. 학교의 목표구조가 어떤 조건에서 어떤 동기적 특성을 가진 교사에게 차별적 영향력을 미치는지에 대한 논의를 제공하였다. This present study explored how perceived school goal structure interact with intrinsic motivation for teaching in predicting teachers` achievement goals. A total of 246 in-service teachers participated in a survey measuring achievement goal for teaching (mastery, performance-approach), perceived school goal structure (mastery, performance), and intrinsic motivation for teaching. The results of multiple regression analyses showed that perceived school mastery goal structure predicted teachers’ endorsement of mastery goals, while perceived performance goal structures predicted teachers` endorsement of performance-approach goals for teaching. In addition, the two types of interaction effects were significant. First, teachers’ intrinsic motivation moderated the relationship between perceived school mastery goal structure and mastery goals for teaching. The results suggested that perceived school mastery goal structure exerted a stronger influence on teachers’ mastery goals for those with low intrinsic motivation than those with high intrinsic motivation. Second, teacher’s intrinsic motivation moderated the relationship between perceived school mastery goal structure and performance-approach goals for teaching. Positive relationship between the two variables was found for teachers with high intrinsic motivation while negative relationship was found for those with low intrinsic motivation. The present study helped to deepen our understanding of under what conditions and for whom perceived school goal structure has a stronger influence on teachers.

학생들의 학급 공동체 의식에 미치는 영향 요인 탐색 : 개인 목표지향성과 교실목표구조의 역할

조윤정(YoonJung Cho) 연세대학교 교육연구소 2015 미래교육학연구 Vol.28 No.1

본 연구는 학생들의 개인적 성취목표지향성과 학생들이 지각한 교실의 성취목표구조가 학급 공동체 의식을 형성하는데 어떤 영향력을 행사하는지를 살펴보았다. 미국 4년제 대학에서 온라인 수업과 전통적 교실 수업을 수강하는 722명의 대학생들이 참여하였다. 위계적 회귀분석 결과, 학생들의 학급 공동체 의식에 가장 큰 영향력을 행사하는 변인은 학생들이 지각한 교실의 숙달-접근 목표구조인 것으로 나타났다. 이를 이어서, 개인의 숙달-접근 목표, 개인 숙달-회피 목표가 학생들의 학급 공동체 의식을 유의미하게 예측하였다. 또한, 개인 숙달-접근 목표와 교실 수행-접근 목표구조 변인이 유의미하게 상호작용하면서 학생들의 학급 공동체 인식에 영향을 미치는 것으로 나타났다. 상호작용 효과를 분석해보면, 숙달-접근 목표를 개인적으로 지향하는 학생이 수행-접근 목표를 강조하는 교실환경에 있을 경우, 학급 공동체 인식 을 형성하기 힘들다는 것을 보여주었다. 나아가서, 학급 공동체 인식은 여러 하위 개념 요소를 포함하는 개념이므로, 정준상관분석을 통해 학생들의 개인적 목표지향성과 지각된 교실의 목표구조가 어떻게 관련되는지를 심층적으로 살펴보았다. 정준상관분석 결과, 학생들의 개인적 목표지향성과 그들이 인지한 교실의 목표구조가 어떤 성격을 갖는가에 따라서 다양한 통로를 거쳐서 학급 공동체 의식을 형성하게 됨을 보여주었다. In this study, we investigated the independent and interaction effects of students’personal goal orientation and perception of classroom goal structure on their classroom sense of community. The participants were 722 undergraduate students enrolled in either online or face-to-face classes. Hierarchical regression analysis revealed that classroom mastery-approach goal structure was the strongest predictors of students’classroom sense of community, followed by personal mastery-approach goal, and personal mastery-avoidance goal being significant predictors. Significant interaction effects emerged between personal mastery-approach goal and classroom performance-approach goal structure, indicating that individuals with mastery-approach goals may be challenged in developing classroom sense of community when a classroom was perceived as having a salient performance-approach goal structure. Canonical correlation analyses were conducted to further examine how achievement goal orientation and classroom goal structure variables are specifically related to five conceptual dimensions of classroom sense of community. The results suggested that students with different goal orientations or perceptions of classroom goal structure may take different pathways to experience classroom sense of community.

대학생들의 미래 삶의 목표가 학업 참여와 소진에 미치는 영향

조윤정(YoonJung Cho),심성옥(Sungok Serena Shim),황소리(Sori Hwang),Helsper,Addison 숭실대학교 영재교육연구소 2023 Global Creative Leader Vol.13 No.4

본 연구에서는 308명의 중국 의과대학 학생들을 대상으로 미래 삶의 목표와 학업 참여 및 소진 경험 간의 관계에서 자기결정적 동기의 매개효과를 살펴보았다. 경로모형 분석결과, 미래 삶의 목표(재정적 성공, 사회적 지위, 공동체적 목표)는 다양한 수준의 자기 결정적 동기와 관련이 있으며, 결과적으로 학업 참여와 소진에 영향을 미치는 것으로 나타났다. 재정적 목표는 통제된 동기를 매개로 학업적 소진을 유의미하게 증가시켰다. 사회적 지위 목표는 자율적 동기를 높여 학업 참여에 긍정적인 영향을 미치지만, 통제된 동기를 매개로 소진을 증가시키는 것으로 나타났다. 반면, 공동체적 목표는 자율적 동기를 높여 학업 참여도를 높이는 동시에 감소된 무동기를 매개로 소진 경험을 줄이는 것으로 나타났다. 이러한 연구결과는 학생들의 현재 학업 동기와 참여도를 이해하는 데 있어 학생들의 미래 인생 목표가 중요한 역할을 한다는 점을 강조한다. The current study investigated the mediating role of self-determined motivation in the relationship between future life goals and academic engagement and burnout of 308 Chinese Medical University students. The path analysis results revealed that distinct future life goals (financial success, social status, and communal goals) corresponded to distinct levels of self-determined motivation. These motivational levels subsequently impacted both academic engagement and burnout. Financial goals predicted heightened burnout through a pathway of controlled motivation. Social status goals, while positively influencing academic engagement via increased autonomous motivation, also contributed to elevated burnout through controlled motivation. On the contrary, communal goals amplified academic engagement through augmented autonomous motivation while simultaneously reducing burnout by diminishing amotivation. These findings emphasize the significant role of students’ future life goals in understanding their current academic motivation and engagement.

Titanium Dioxide/UV Photocatalytic Disinfection in Fresh Carrots

CHO, MIHEE,CHOI, YOONJUNG,PARK, HYOJIN,KIM, KWANSIK,WOO, GUN-JO,PARK, JIYONG ational Association for Food Protection 2007 Journal of food protection Vol.70 No.1

<P>Increased occurrences of fresh produce-related outbreaks of foodborne illness have focused attention on effective washing processes for fruits and vegetables. A titanium dioxide (TiO2) photocatalytic reaction under UV radiation provides a high rate of disinfection. The photo-killing effects of TiO2 on bacteria in liquid cultures under experimental conditions have been widely studied. However, the disinfection effects of the TiO2 photocatalytic reaction on fresh vegetables during a washing process have not been evaluated. Our objectives were to design a pilot-scale TiO2/UV photocatalytic reactor for fresh carrots and to compare the bactericidal effects of the TiO2/UV reaction against bacteria in liquid media and on carrots. TiO2/UV photocatalytic reactions for 40, 60, and 30 s were required for the complete killing of Escherichia coli, Salmonella Typhimurium, and Bacillus cereus (initial counts of approximately 6.7 log CFU/ml), respectively. The counts of total aerobic bacteria in fresh carrots and foodborne pathogenic bacteria in inoculated carrots were also measured. Counts of total aerobic bacteria were reduced by 1.8 log CFU/g after TiO2/UV photocatalytic disinfection for 20 min compared with a 1.1-log CFU/g reduction by UV alone. E. coli, Salmonella Typhimurium, and B. cereus (8 log CFU/ml) were inoculated onto carrots, and the number of surviving bacteria in carrots was determined after treatment. The TiO2/UV treatment exhibited 2.1-, 2.3-, and 1.8-log CFU/g reductions in the counts of E. coli, Salmonella, Typhimurium, and B. cereus, respectively, compared with 1.3-, 1.2-, and 1.2-log CFU/g reductions by UV alone. The TiO2/UV photocatalyst reaction showed significant bactericidal effects, indicating that this process is applicable to nonthermal disinfection of fresh vegetables.</P>

Cost-Effectiveness Analysis of Three Diagnostic Strategies for the Detection of EGFR Mutation in Advanced Non-Small Cell Lung Cancer

Cho Sun-Mi,Lee Hye Sun,Jeon Soyoung,Kim Yoonjung,Kong Sun-Young,Lee Jin Kyung,Lee Kyung-A 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.6

Background: In non-small cell lung cancer (NSCLC), epidermal growth factor receptor (EGFR) mutation testing of tumor tissue should be conducted at diagnosis. Alternatively, circulating tumor DNA can be used to detect EGFR mutation. We compared the cost and clinical effect of three strategies according to the application of the EGFR test. Methods: Decision models were developed to compare the cost-effectiveness of tissue-only, tissue-first, and plasma-first diagnostic strategies as first- and second-line treatments for NSCLC from the perspective of the Korean national healthcare payer. Progression-free survival (PFS), overall survival (OS), and direct medical costs were assessed. A one-way sensitivity analysis was performed. Results: The plasma-first strategy correctly identified numerous patients in the first- and second-line treatments. This strategy also decreased the cost of biopsy procedures and complications. Compared with that when using the other two strategies, the plasma-first strategy increased PFS by 0.5 months. The plasma-first strategy increased OS by 0.9 and 1 month compared with that when using the tissue-only and tissue-first strategies, respectively. The plasma-first strategy was the least expensive first-line treatment but the most expensive second-line treatment. First-generation tyrosine kinase inhibitor and the detection rate of the T790M mutation in tissues were the most cost-influential factors. Conclusions: The plasma-first strategy improved PFS and OS, allowing for a more accurate identification of candidates for targeted therapy for NSCLC and decreased biopsy- and complication-related costs.

Enigma negatively regulates p53 through MDM2 and promotes tumor cell survival in mice

Jung, Cho-Rok,Lim, Jung Hwa,Choi, Yoonjung,Kim, Dae-Ghon,Kang, Koo Jeong,Noh, Seung-Moo,Im, Dong-Soo American Society for Clinical Investigation 2010 The Journal of clinical investigation Vol.120 No.12

Comparisons of the pharmacokinetics and tolerability of fixed-dose combinations of amlodipine besylate/losartan and amlodipine camsylate/losartan in healthy subjects: a randomized, open-label, single-dose, two-period, two-sequence crossover study

Choi, YoonJung,Lee, SeungHwan,Cho, Sang-Min,Kang, Won-Ho,Nam, Kyu-Yeol,Jang, In-Jin,Yu, Kyung-Sang Dove Medical Press 2016 Drug design, development and therapy Vol.10 No.-

<P><B>Background</B></P><P>A fixed-dose combination (FDC) of amlodipine and losartan has been used to reduce blood pressure in patients whose hypertension is not sufficiently controlled with either drug alone. The aim of this study was to evaluate the pharmacokinetic (PK) characteristics and tolerability of an FDC of 6.94 mg amlodipine besylate (5 mg as amlodipine)/50 mg losartan potassium compared to an FDC of 5 mg amlodipine camsylate/50 mg losartan potassium in healthy subjects.</P><P><B>Subjects and methods</B></P><P>A randomized, open-label, single-dose, two-period, two-sequence crossover study was conducted on 46 healthy male subjects. Blood concentrations were measured by liquid chromatography–tandem mass spectrometry. Blood samples were collected up to 144 hours post dose for each period. PK parameters were calculated in each treatment group using a noncompartmental method. The 90% confidence intervals (CIs) of the geometric mean ratios of the two treatments for the maximum plasma concentration (<I>C</I><SUB>max</SUB>) and the area under the concentration curve from time zero to the last quantifiable time point (AUC<SUB>0–t</SUB>) were estimated. Tolerability assessments were performed for all subjects who received the drug at least once.</P><P><B>Results</B></P><P>The PK profiles of the two treatments were similar. For amlodipine, the geometric mean ratios (90% CIs) of amlodipine besylate to amlodipine camsylate for the <I>C</I><SUB>max</SUB> and AUC<SUB>0–t</SUB> were 0.98 (0.94−1.01) and 0.97 (0.93−1.01), respectively. The corresponding values for losartan were 0.91 (0.81−1.02) and 1.05 (0.98−1.12), respectively. The incidence of adverse events was not significantly different between the two treatments, and both were well tolerated.</P><P><B>Conclusion</B></P><P>An FDC of 6.94 mg amlodipine besylate (5 mg as amlodipine)/50 mg losartan potassium produced similar results to an FDC of 5 mg amlodipine camsylate/50 mg losartan potassium treatment with respect to the PK parameters of amlodipine and losartan based on <I>C</I><SUB>max</SUB> and AUC<SUB>0–t</SUB> values. The amlodipine besylate/losartan potassium combination was well tolerated by healthy male subjects.</P>