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Fan Yang,Hairong Xiong,Li Duan,Qian Li,Xin Li,Yongqin Zhou 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4
Purpose MicroRNAs (miRNAs) are a group of small non-coding RNAs involved in different cancers, including lung cancer. Here, we aim to investigate the expression profiles of circulating miRNAs and their roles contributed to the progress of lung cancer. Materials and Methods The levels of circulating miRNA in lung cancer patients were investigated by miRNAs assay. Then we predicted the target genes of aberrantly expressing miRNAs by searching genetic databases. Based on the A549 cells transfected with miR-1246 mimics or miR-1246 inhibitor, we further measured the roles of miR-1246 involving in the epithelial-mesenchymal transition (EMT), migration and invasion capacities of lung cancer cells in vitro. Finally, we detected the effects of miR-1246 on glycogen synthase kinase-3β (GSK-3β)/β-catenin pathway by immunofluorescence and Western blot, respectively. Results We identified that 14 miRNAs were aberrantly expressed in the serum of lung cancer patients. Among them, miR-1246 was the most up-regulated. The cell assays indicated that miR-1246 significantly increased the migration and invasion capabilities of A549 lung cancer cells. Meanwhile, immunofluorescence analysis revealed that miR-1246 promoted EMT process of A549 cells accompanying with decreasing E-cadherin expression, while increasing vimentin and transforming growth factor β expression. Furthermore, an online tool predicated that miR-1246 might bind to 3'-untranslated region of GSK-3β, which was confirmed by overexpression and knockdown of miR-1246 assays. Conclusion Taken together, the study illustrates that miR-1246 regulates Wnt/β-catenin pathway through targeting GSK-3β/β-catenin, which partly contributing to tumor metastasis. MiR- 1246 may play an essential role in the diagnosis and therapeutic of lung cancer.
Yongqin Pan,Genlong Jiao,Jingge Yang,Rui Guo,Jinyi Li,Cunchuan Wang 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.3
Recurrent laryngeal nerve (RLN) injury can result in unilateral or bilateral vocal cords paralysis, thereby causing a series of complications, such as hoarseness and dyspnea. However, the repair of RLN remains a great challenge in current medicine. This study aimed to develop human umbilical mesenchymal stem cells (HuMSCs) and nerve growth factor (NGF)-loaded heparinized collagen scaffolds (HuMSCs/NGF HC-scaffolds) and evaluate their potential in the repair of RLN injury. HuMSCs/NGF HC-scaffolds were prepared through incorporating HuMSCs and NGF into heparinized collagen scaffolds that were prefabricated by freeze-drying in a template. The resulting scaffolds were characterized by FTIR, SEM, porosity, degradation in vitro, NGF release in vitro and bioactivity. A rabbit RLN injury model was constructed to appraise the performance of HuMSCs/NGF HC-scaffolds for nerve injury repair. Electrophysiology, histomorphology and diagnostic proteins expression for treated nerves were checked after application of various scaffolds. The results showed that the composite scaffolds with HuMSCs and NGF were rather helpful for the repair of broken RLN. The RLN treated with HuMSCs/NGF HC-scaffolds for 8 weeks produced a relatively normal electromyogram, and the levels of calciumbinding protein S100, neurofilament and AchE pertinent to nerve were found to be close to the normal ones but higher than those resulted from other scaffolds. Taken together, HuMSCs/NGF HC-scaffolds exhibited a high score on the nerve injury repair and may be valuable for the remedy of RLN injury.
Intensifying sulforaphane formation in broccoli sprouts by using other cruciferous sprouts additions
Hao Liang,Yongqin Wei,Ruimin Li,Li Cheng,Qipeng Yuan,Fuping Zheng 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4
Sulforaphane is a significant chemopreventive compound which is the predominant glucosinolate in broccoli sprouts. However, the existence of the epithiospecifier protein could direct the hydrolysis of glucosinolates toward sulforaphane nitrile formation instead of sulforaphane. Therefore, the study aimed on improving the yielding of sulforaphane in broccoli sprouts with a new method of the united hydrolysis of cruciferous sprouts. According to the results, the addition of radish, rocket and rape sprouts to broccoli sprouts could promote the hydrolysis of the glucoraphanin to anticancer effective sulforaphane to 2.03, 2.32 and 1.95-fold, respectively, compared to single broccoli sprouts. Meanwhile, the formation of non-bioactive sulforaphane nitrile in these three groups decreased greatly. However, the addition of mustard sprouts had no positive effect. These observations could make a contribution to the potential chemoprotective effects of broccoli sprouts.
Lin Wang,Chongzhi Wu,Yongqin Li,Hanlong Liu,Wengang Zhang,Xiang Chen 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.12
Probabilistic risk assessment of slope failure evaluates the slope safety in a quantitative manner, which considers the failure probability and failure consequence simultaneously. However, risk assessment of unsaturated slope accounting for spatially variable soil-water characteristic curve (SWCC) model parameter and saturated hydraulic conductivity has been rarely reported. A probabilistic risk assessment approach is proposed in current study for rationally quantifying the unsaturated slope failure risk with the aid of Monte Carlo (MC) simulation. The SEEP/W and SLOPE/W modules contained in Geostudio software are applied to carry out deterministic analysis, where factor of safety (FS) of the unsaturated slope is calculated by Morgenstern–Price method. The spatially variable hydraulic parameters are characterized by their respective random fields that are transferred from the random void ratio field in this study, rather than generating them separately. The proposed approach is subsequently employed to an unsaturated slope example for exploring the influences of spatially variable void ratio. Results show that the unsaturated slope failure risk is considerably affected by the spatially variable void ratio, and the single exponential autocorrelation function (ACF) popularized in geotechnical engineering tends to underestimate the failure risk in the unsaturated slope risk assessment.
Therapeutic inhibition of SGK1 suppresses colorectal cancer
Xuchun Liang,Chunling Lan,Guanming Jiao,Wencheng Fu,Xuesha Long,Yu An,Kejin Wang,Jinzhe Zhou,Ting Chen,Yongqin Li,Jiahong Xu,Qi Huang,Bin Xu,Junjie Xiao 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Colorectal cancer (CRC) is one of the leading causes of death worldwide. Thus, the development of new therapeutic targets for CRC treatment is urgently needed. SGK1 is involved in various cellular activities, and its dysregulation can result in multiple cancers. However, little is known about its roles and associated molecular mechanisms in CRC. In present study, we found that SGK1 was highly expressed in tumor tissues compared with peri-tumor samples from CRC patients. In vitro experiments revealed that SGK1 overexpression promoted colonic tumor cell proliferation and migration and inhibited cell apoptosis induced by 5-fluorouracil (5-FU), while SGK1 shRNA and inhibitors showed the inverse effects. Using CRC xenograft mice models, we demonstrated that knockdown or therapeutic inhibition of SGK1 repressed tumor cell proliferation and tumor growth. Moreover, SGK1 inhibitors increased p27 expression and promoted p27 nuclear accumulation in colorectal cancer cells, and p27 siRNAs could attenuate the repression of CRC cell proliferation induced by SGK1 inhibitors. Collectively, SGK1 promotes colorectal cancer development via regulation