The potential molecular effects of bursal septpeptide II on immune induction and antitumor activity

Guang Fang Zhou,Qing Tao Liu,Bin Zhou,Ya Feng Qiu,Xiao Dong Liu,Zhi Yong Ma,Xiu Li Feng,Rui Bing Cao,Pu Yan Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

The bursa of Fabricius (BF) is the acknowledged central humoral immune organ in birds. Bursal septpeptide II (BSP-II) is animmunomodulatory bioactive peptide isolated from BF. To understand the effects of BSP-II on immune induction, gene expression profilesof hybridoma cells treated with BSP-II were evaluated. Pathway analysis showed that regulated genes were involved in cytokine-cytokinereceptor interactions, T cell receptor signaling pathway, and pathway in cancer. It was observed that BSP-II reduced tumor cells proliferationand stimulated p53 expression. These results indicate potential mechanisms underlying the effects of the humoral immune system on immuneinduction, including antitumor activities. Our study has provided a novel insight into immunotherapeutic strategies for treating human tumors.

Pseudo-homogeneous kinetic modeling of dioctyl terephthalate (DOTP) production by esterification of terephthalic acid and 2-ethylhexanol over tetrabutyl titanate catalyst

Feng Zhou,Jinjin Cai,Xiaoning Mao,Zhenyu Wu,Yong Nie 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9

As a green plasticizer, the industrial production of dioctyl terephthalate (DOTP) is still facing the problemof high energy consumption. To optimize the production process and reactor, it is essential to understand the kineticbehavior of reaction system. In this work, the two-step consecutive esterification of solid terephthalic acid (PTA) and 2-ethylhexanol (2-EH) catalyzed by tetrabutyl titanate was studied. First, the equilibrium constants and enthalpies of thetwo-step reaction were experimentally determined and validated by the group contribution methods. Then, a pseudohomogeneouskinetic model was developed, and the reaction order of PTA was corrected to reflect its solid phase characteristic. Non-isothermal kinetic experiments were carried out under different initial feed molar ratios and catalystconcentrations, and the kinetic parameters in the model were estimated by mathematical regression. The model predicteddata agreed well with the experimental data. Finally, the analyses of reaction rate showed that the first-step reactionwas the rate-controlling step of the whole esterification process.

Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy

Zhou Jing,Feng Ji,Wu Yong,Dai Hui-Qi,Zhu Guang-Zhi,Chen Pan-Hong,Wang Li-Ming,Lu Guang,Liao Xi-Wen,Lu Pei-Zhi,Su Wen-Jing,Hooi Shing Chuan,Ye Xin-Pin,Shen Han-Ming,Peng Tao,Lu Guo-Dong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Transarterial chemoembolization (TACE) is the first-line treatment for unresectable intermediate-stage hepatocellular carcinoma (HCC). It is of high clinical significance to explore the synergistic effect of TACE with antiangiogenic inhibitors and the molecular mechanisms involved. This study determined that glucose, but not other analyzed nutrients, offered significant protection against cell death induced by sorafenib, as indicated by glucose deprivation sensitizing cells to sorafenib-induced cell death. Next, this synergistic effect was found to be specific to sorafenib, not to lenvatinib or the chemotherapeutic drugs cisplatin and doxorubicin. Mechanistically, sorafenib-induced mitophagy, as indicated by PINK1 accumulation, increased the phospho-poly-ubiquitination modification, accelerated mitochondrial membrane protein and mitochondrial DNA degradation, and increased the amount of mitochondrion-localized mKeima-Red engulfed by lysosomes. Among several E3 ubiquitin ligases tested, SIAH1 was found to be essential for inducing mitophagy; that is, SIAH1 silencing markedly repressed mitophagy and sensitized cells to sorafenib-induced death. Notably, the combined treatment of glucose restriction and sorafenib abolished ATP generation and mitophagy, which led to a high cell death rate. Oligomycin and antimycin, inhibitors of electron transport chain complexes, mimicked the synergistic effect of sorafenib with glucose restriction to promote cell death mediated via mitophagy inhibition. Finally, inhibition of the glucose transporter by canagliflozin (a clinically available drug used for type-II diabetes) effectively synergized with sorafenib to induce HCC cell death in vitro and to inhibit xenograft tumor growth in vivo. This study demonstrates that simultaneous treatment with sorafenib and glucose restriction is an effective approach to treat HCC, suggesting a promising combination strategy such as transarterial sorafenib-embolization (TASE) for the treatment of unresectable HCC.

[네트워크 프로토콜] 무선LAN 환경에서 TDMA방식을 이용한 동일채널 간섭 감소

Feng Zhou,최용훈(Yong-Hoon Choi),박수원(Suwon Park),이승형(Seung Hyong Rhee) 한국정보과학회 2012 정보과학회논문지 : 정보통신 Vol.39 No.2

동일 채널 간섭은 주파수가 같은 동일 채널에서 둘 또는 그 이상의 전파가 수신기에서 간섭하여 장애가 발생하는 현상이다. 본 논문에서는 고밀도의 무선랜 환경에서 동일 채널 간섭 문제를 연구하고 TDMA(Time Division Multiplexing Access) 알고리즘을 이용하여 개선방법을 제안한다. 먼저 무선랜 개발과정에서 핵심기술문제를 언급하고 동일채널 간섭의 연구 배경을 제시한다. 그 후 네트워크 성능에 영향을 많이 주는 MAC(Media Access Control) 프로토콜을 설명하고 분석한다. 마지막으로 간섭 현상 시나리오를 통해 제안한 메커니즘을 이용하여 기존의 CSMA/CA(Carrier Sense Multiple Access/Collision Avoidance) 메커니즘과 비교한다. 시뮬레이션을 통하여 개선된 성능을 확인한다. Co-channel interference is a phenomenon that two or more radio wave interfere each other in same geographic area transmit on the same frequency. In this paper, we discuss the problem of co-channel interference in high-density wireless LAN(Local Area Network) environment and propose a way to improve throughput and delay by using TDMA(Time Division Multiplexing Access) algorithm based on WLAN. First, the key technical problem of WLAN during its development process is pointed out and the research background of co-channel interference in WLAN is offered; then we analyze MAC layer protocol of IEEE 802.11b standard, which mainly affects system throughputs and channel quality. Based on a half-coverage area scenario, we provide the proposed mechanism with TDMA-based scheme. The simulation results show network throughput improvement and transmission delay reduction comparing with original CSMA/CA(Carrier Sense Multiple Access/Collision Avoidance) with RTS/CTS(Ready-To-Send/Clear-To-Send) mechanism. Finally, we summarize the achievement that we have made.

Expression and Immunogenicity of SARSCoV-2 Virus-Like Particles based on Recombinant Truncated HEV-3 ORF2 Capsid Protein

( Yong-fei Zhou ),( Jiao-jiao Nie ),( Chao Shi ),( Ke Ning ),( Yu-feng Cao ),( Yanbo Xie ),( Hongyu Xiang ),( Qiuhong Xie ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

COVID-19 is an emerging disease that poses a severe threat to global public health. As such, there is an urgent demand for vaccines against SARS-CoV-2, the virus that causes COVID-19. Here, we describe a virus-like nanoparticle candidate vaccine against SARS-CoV-2 produced by an E. coli expression system. The fusion protein of a truncated ORF2-encoded protein of aa 439~608 (p170) from hepatitis E virus CCJD-517 and the receptor-binding domain of the spike protein from SARSCoV-2 were expressed, purified and characterized. The antigenicity and immunogenicity of p170-RBD were evaluated in vitro and in Kunming mice. Our investigation revealed that p170-RBD selfassembled into approximately 24 nm virus-like particles, which could bind to serum from vaccinated people (p < 0.001) and receptors on cells. Immunization with p170-RBD induced the titer of IgG antibody vaccine increased from 14 days post-immunization and was significantly enhanced after a booster immunization at 28 dpi, ultimately reaching a peak level on 42 dpi with a titer of 4.97 log10. Pseudovirus neutralization tests showed that the candidate vaccine induced a strong neutralizing antibody response in mice. In this research, we demonstrated that p170-RBD possesses strong antigenicity and immunogenicity and could be a potential candidate for use in future SARS-CoV-2 vaccine development.

Increased retinoic acid signaling decreases lung metastasis in salivary adenoid cystic carcinoma by inhibiting the noncanonical Notch1 pathway

Zhou Meng-jiao,Yang Jia-jie,Ma Ting-yao,Feng Ge-xuan,Wang Xue-lian,Wang Li-Yong,Ge Yu-ze,Gao Ran,Hong-liang Liu,Shan Lin,Kong Lu,Chen Xiao-hong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

MYB-NFIB fusion and NOTCH1 mutation are common hallmark genetic events in salivary gland adenoid cystic carcinoma (SACC). However, abnormal expression of MYB and NOTCH1 is also observed in patients without MYB-NFIB fusion and NOTCH1 mutation. Here, we explore in-depth the molecular mechanisms of lung metastasis through single-cell RNA sequencing (scRNA-seq) and exome target capture sequencing in two SACC patients without MYB-NFIB fusion and NOTCH1 mutation. Twenty-five types of cells in primary and metastatic tissues were identified via Seurat clustering and categorized into four main stages ranging from near-normal to cancer-based on the abundance of each cell cluster in normal tissue. In this context, we identified the Notch signaling pathway enrichment in almost all cancer cells; RNA velocity, trajectory, and sub-clustering analyses were performed to deeply investigate cancer progenitor-like cell clusters in primary tumor-associated lung metastases, and signature genes of progenitor-like cells were enriched in the “MYC_TARGETS_V2” gene set. In vitro, we detected the NICD1-MYB-MYC complex by co-immunoprecipitation (Co-IP) and incidentally identified retinoic acid (RA) as an endogenous antagonist of genes in the “MYC_TARGETS_V2” gene set. Following this, we confirmed that all-trans retinoic acid (ATRA) suppresses the lung metastasis of SACC by correcting erroneous cell differentiation mainly caused by aberrant NOTCH1 or MYB expression. Bioinformatic, RNA-seq, and immunohistochemical (IHC) analyses of primary tissues and metastatic lung tissues from patients with SACC suggested that RA system insufficiency partially promotes lung metastasis. These findings imply the value of the RA system in diagnosis and treatment.

Design, performance analysis, and testing of a GFRP racetrack ring for a high-temperature superconducting motor with a flexible support rotor

Yong Zhou,Feng Xie,Hai-Yuan Jia,Wei Chen 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.10

A conceptual 20 MW high-temperature superconducting (HTS) motor (HTSM) with a novel glass-fiber reinforced plastic (GFRP) racetrack ring in the flexible support rotor was explored in this study. To support the cold mass at operating conditions, the high-strength, lowleakage GFRP racetrack ring was designed as follows. First, conceptual design of the 20 MW HTSM was conducted, and the torque transmitted by the GFRP racetrack rings was obtained through electromagnetic analysis. Second, a GFRP racetrack ring was designed, and its total cross-sectional area was obtained through theoretical analysis. Third, thermal leakage and stress were calculated and examined through theoretical and finite element analyses, respectively. The GFRP racetrack ring with the “D” ring was selected based on the results. The thickness and width of the GFRP ring were set to 16 and 10 mm, respectively, and the thickness of the “D” ring was 3 mm. Then, a full-scaled GFRP ring was fabricated, and its strength and stiffness were tested via room-temperature and low-temperature tensile tests. The average tensile strength and average Young’s modulus of the GFRP ring at room temperature were 1131.5 MPa and 49.9 GPa, respectively. The strength of the GFRP ring at 193.4 K was 1243.9 MPa, and the Young's modulus of the GFRP ring at 141.3 K was 69 GPa. Lastly, a new 200 kW HTS generator was assembled using a scaled rotor with the same structure as the 20 MW HTSM and the original stator of an existing 200 kW generator. The test system was built to evaluate the feasibility of the GFRP ring before manufacturing the 20 MW HTSM. Test results showed that the GFRP ring is feasible, the design method of the GFRP ring is credible. The GFRP ring is suitable for the cold mass support of HTSMs and other low-temperature devices.

Prevalence and Age, Gender and Geographical Area Distribution of Esophageal Squamous Cell Carcinomas in North China from 1985 to 2006

Feng, Xiao-Shan,Yang, Yan-Tong,Gao, She-Gan,Ru, Yi,Wang, Gong-Ping,Zhou, Bo,Wang, Yu-Feng,Zhang, Peng-Fei,Li, Pu-Yu,Liu, Yong-Xuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Objective: To establish the prevalence and distribution profile of esophageal squamous cell carcinomas (ESCCs) over a 22-yr period in North China. Methods: Using endoscopy for primary diagnosis and histological analysis for the further confirmation, a total of 74,854 ESCC patients aged 20-89 between January 1985 and December 2006 were investigated to analyze the epidemiological profile including prevalence rates, distribution of age-of-onset, gender and geographical area of ESCC in Luoyang, the highest incidence area of North China. Results: A total of 4092 cases of ESCC were finally diagnosed among 74,854 patients who had their first endoscopies. The prevalence among males was higher than that among females (p<0.01), resulting in an overall male:female OR of 1.2 (95%CI, 1.2-1.3). The prevalence in rural areas was higher than in urban areas (p<0.01), resulting in an overall rural:urban OR of 2.6 (95%CI, 2.4-2.9). The rural:urban ORs and the 95% CI increased continuously from 2.6, 2.3-3.0 to 2.7, 2.2-3.3, respectively, for 4 consecutive periods during the 22-yr study period. Moreover, the median age of onset among females was higher than that among males (p<0.01). For both sexes and in both areas, the prevalence rates declined and the median age of onset rose for 4 consecutive periods in the 22-yrs time frame (p<0.01). Conculsions: These data reveal the epidemiological profile of ESCC in the area of North China, and suggest that urban areas and rural people account for a growing proportion of the ESCC patients although the prevalence of ESCC significantly declined and the median age-of-onset postponed over the 22-yrs period. Moreover, the prevalence status of ESCC in rural areas also underlines the need for public health initiatives aimed at reducing risk factors of this fatal disease.

FE MODEL UPDATING OF ROTOR SHAFT USING OPTIMIZATION TECHNIQUES

Yong Han Kim(김용한),Fu Zhou Feng,Bo-Suk Yang(양보석) 한국소음진동공학회 2003 한국소음진동공학회 학술대회논문집 Vol.2003 No.11

FACTORS CONTROLLING THE STRONGEST SIZES IN THE INVERSE HALL-PETCH RELATIONSHIP

YONG PAN,ZHAO FENG ZHOU,SHAO-YUN FU,YANGUANG NIE,CHANG Q SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2008 NANO Vol.3 No.3

Incorporating the bond-order-length-strength correlation mechanism [Sun CQ, Prog Solid State Chem 35, 1 (2007)] and Born’s criterion for melting [J. Chem. Phys. 7, 591 (1939)] into the conventional Hall-Petch relationship has turned out an analytical expression for the size and temperature dependence of the mechanical strength of nanograins, known as the inverse Hall-Petch relationship (IHPR). Reproduction of the measured IHPR of Ni, NiP and TiO2 nanocrystals revealed that: (i) the competition between the size-induced energy-density gain and atomic cohesive energy loss in the surface skins of nanograins originate the IHPR; (ii) the competition between the activation and inhibition of atomic dislocations motion activate the entire IHPR behavior; (iii) the bond nature involved and the T/Tm ratio between the temperature of operating and the temperature of melting dictate the measured strongest sizes of a given specimen; (iv) a quasimolten phase presenting before melting determines the size-induced softening and the superplasticity of nanostructures.