소 바이러스성 설사병 바이러스 gp53 항원부위 유전자의 재조합 및 염기서열 연구

Yeo, Sang-geon,Cho, HJ,Masri, SA 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.2

Molecular cloning and nucleotide sequencing were undertaken for the RNA genome of gp53 antigenic region in cytopathic Singer strain of bovine viral diarrhea virus. The cloned cDNA was 939 nucleotides in length having a base composition of 31.0% A, 19.6% C, 25.5% G and 24.0% T. The sequence was corresponded to approximately 77.8%(817 bases) of predicted gp53 region and 122 bases after 3'end of gp53 region in the Singer strain when compared with NADL strain of known sequence. A single open reading frame was found in the sequence of 2nd frame and was deduced as encoding 312 amino acids.

Studies on Production of Monoclonal Antibody to Bovine Immunoglobulin G_1 : Ⅰ. 산양의 발정유기, 단발정주기 및 과잉 배관에 관하여

YEO, SANG GEON,KIM, JUNG WOO,LEE, HYO JONG 경상대학교 유전공학연구소 1986 遺傳工學硏究所報 Vol.5 No.-

In order to produce monoclonal antibody to bovine IgG_1, attempts were made to isolate and purify the bovine IgG_1 from colostrum, and the prepared IgG_1 heavy chain was used as an antigen for the immunization of BALB/c mice. Spleen cells from BALB/c mice immunized with bovine IgG_1 heavy chain were successfully fused with SP2/O mouse myeloma cells. The antibody produced by the hybrid cells was specified by enzyme-linked immunosorbent assay. Of 48 initial hybridoma clones, 21 produced antibodies to bovine IgG_1. Through cloning by limiting dilutions, 2(G1D2 and G1H12) of these hybrid clones retained specificity and were grown in mass culture. The monoclonal(anti-bovine IgG_1) antibody G1D2 and G1H12 were reacted to bovine IgG_1 specifically in their dilutions of more 10^2- and 10-folds, respectively.

Analysis for nucleotide sequence of the small membrane (sM) protein gene of porcine epidemic diarrhea virus Chinju99 isolated in Korea

Yeo, Sang-Geon,Lee, Changhee The Korean Society of Veterinary Science 2007 大韓獸醫學會誌 Vol.47 No.2

To provide information for the molecular pathogenesis and antigenic structures of Korean isolates of porcine epidemic diarrhea virus (PEDV), the small membrane (sM) protein gene of Chinju99 strain, which was previously isolated from piglets suffering from severe diarrhea was characterized and further analyzed with other PEDV strains. The sM gene of Chinju99 generated by reverse transcription and polymerase chain reaction had a single open reading frame with 231 bases consisting of 24.2% adenine, 18.6% cytosine, 18.1% guanine and 39.0% thymine nucleotides. Nucleotide sequence of the gene revealed 97.8% homology to those of Belgian strain CV777 and British strain Br1/87, and 97.0% to Chinese strain LZC. The gene encoded a protein with 76 amino acids, and putative amino acid sequence of the gene revealed 98.7% homology to those of CV777 and Br1/87, and 96.1% to LZC. The amino acids of Chinju99 sM gene consisted of mostly hydrophobic residues, and there were one potential N-myristylation site and one potential threonine (T)-linked phosphorylation site recognized. Also, there was a transmembrane region with 46 amino acids, and Chinju99 was more close to CV777 and Br1/87 than to LZC in phylogenetic analysis on the sM amino acid sequences.

소 바이러스성 설사병 바이러스의 유전자 재조합 DNA clone의 작성에 관한 연구

Yeo, Sang-geon,Cho, H.J.,Masri, S.A. 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.3

Molecular cloning was carried out on the Danish strain of bovine viral diarrhea virus(BVDV) to construct strategy for the diagnostic tools and effective vaccine of BVD afterwards. A recombinant DNA clone(No. 29) was established successfully from cDNA for viral RNA tailed with adenine homopolymer at 3'-end. $^{32}P$-labeled DNA probes of 300~1,800bp fragments, originating from the clone 29, directed specific DNA-RNA hybridization results with BVDV RNA. Recombinant DNA of the clone 29 was about 5,200bp representing 41.6% of the full length of Danish strain's RNA, and restriction sites were recognized for EcoR I, Sst I, Hin d III and Pst I restriction enzymes in the DNA fragment.

A Medium Maturity, and High Quality Rice Variety, "Hwabongbyeo"

Byeong Geon Oh,Sang Jong Lim,Ki Hwan Yi,No Bong Park,Un Sang Yeo,Sae Jun Yang,Ho Yeong Kim,Heung Gu Hwang,Do Yeon Kwak,Jong Rae Kang,Jeom Sik Lee,Soon Chul Kim,Moo Sang Lim 한국육종학회 2003 한국육종학회지 Vol.35 No.1

This japonica cultivar was developed from a cross between two F1 hybrids of YR13168 (Milyang 95/Iri390) and YR13198 (Milyang101/Iri390) using anther culture. It has about 104 days of growing duration from seeding to heading and relatively short

Review of Glyphosate and Glufosinate Analysis Method of Food Materials Using LC-MS-MS, LC-Q-ToF

Geon-Woo Park,Kyung-Ho Park,Eun-Hyeong Kim,Un Hyung Yeo,Sang-Yun Lee,Sang-gu Kim 한국농약과학회 2020 한국농약과학회 학술발표대회 논문집 Vol.2020 No.11

한국산 겨우살이(Viscum album coloratum)로부터 추출된 lectin의 돼지에 대한 독성 및 오제스키병 백신의 면역원성에 미치는 영향

여상건,Yeo, Sang-Geon 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.3

In the present study toxicity and immunostimulating activity of the lectin(KML-C), which was extracted from Korean mistletoe(Viscum album coloratum) were investigated in swine. To determine the toxicity, lectin was injected into thigh or cervical muscles of 4-week-old piglets(Landrace) and observed clinically and pathologically. For determination of the immnunostimulating activity, lectin($0.7{\mu}g/kg$ of body weight)-adjuvanted vaccine of Aujeszky's disease virus(ADV)(NYJ1-87) which was inactivated by 0.2% formalin was injected into the cervical muscle of antibody-negative piglets in the same age group. Subpopulation of the immune cells and serum neutralizing(SN) antibodies in the piglets were examined after vaccination, and resistance of the piglets against challenge by virulent NYJ1-87 was further examined. The results were also compared with those from piglets injected with aluminum hydroxide [$Al(OH)_3$]-adjuvanted vaccine of inactivated NYJ1-87 and NYJ1-87 vaccine without adjuvant, and the results are as follows. By injection of lectin with $30{\mu}g/kg$ of body weight to the thigh muscle, all of 12 piglets died after signs such as dyspnea, fever, systemic erythema and subcutaneous hemorrhages, and lesions pertaining to poisonous hepatitis and dysfunction of kidney were observed. By injection of lectin with $7{\mu}g/kg$ of body weight to the thigh muscle, all of 12 piglets showed signs such as edema and cutaneous hemorrhage in the injected area, lameness and depression, and lesions pertaining to poisonous hepatitis and dysfunction of kidney were observed. By injection of lectin with 1, 3 and $5{\mu}g/kg$ of body weight to the thigh muscle of each one piglet, signs such as congestion, induration and grayish coloration in the injected area, depression and inappetence were observed in all piglets. Toxic changes were also observed in the liver and kidney of piglets by lectin of 3 and $5{\mu}g$. By injection of lectin with 0.5 and $0.7{\mu}g/kg$ of body weight to the cervical muscle of each 9 piglets, all piglets were clinically normal and there were no significant changes in blood counts and chemistry values. Whereas, epithelial swelling and vacuolation of convoluted tubules were observed from one piglet injected with lectin of $0.7{\mu}g$, and necrosis and fibrosis of muscular fiber were observed in the muscle of one piglet injected with lectin of $0.5{\mu}g$. Only population of sIgM+ B lymphocytes increased among immune cells in all of 15 piglets immunized with lectin($0.7{\mu}g/kg$ of body weight)-adjuvanted vaccine, while compared to those in $Al(OH)_3$-adjuvanted vaccine and vaccine without adjuvant. No additional stimulation to the immune cells was recognized when lectin was added to $Al(OH)_3$-adjuvanted vaccine. In piglets immunized with lectin-adjuvanted vaccine, SN titers in reciprocal values for loge were 1.3-4.0 at 1-4 weeks after vaccination, which was similar to those with 1.0-3.3 by vaccine without adjuvant but lower than those with 2.0-5.7 by $Al(OH)_3$-adjuvanted vaccine. Also, no additional increase in the SN titers was recognized when lectin was added to $Al(OH)_3$-adjuvanted vaccine. Piglets immunized with lectin-adjuvanted vaccine were resistant to challenge by the virulent NYJ1-87 at 4 weeks after vaccination, and the SN titers reached to 5.0 one week after challenge, which was higher than those with 4.0 by vaccine without adjuvant but somewhat lower than those with 7.7 by $Al(OH)_3$-adjuvanted vaccine.

블루투스와 와이파이를 이용한 스마트홈 제어 시스템

여상삼(Sang-Sam Yeo),이원호(Won-Ho Lee),박성용(Seong-Yong Park),박건희(Geon-Hui Park),조다혜(Da-Hye Jo),구준영(Jun-yeong Koo) 한국컴퓨터정보학회 2020 한국컴퓨터정보학회 학술발표논문집 Vol.28 No.2

GIS와 치수경제성 분석을 이용한 도시홍수방어수위 설정

신상영(Sang Young Shin),이창희(Chang Hee Lee),이양재(Yang Jae Lee),여창건(Chang Geon Yeo) 대한공간정보학회 2007 한국지형공간정보학회 학술대회 Vol.2007 No.6

침수 피해를 방지하고 경감하기 위해서는 침수범위와 침수심을 예측하여 예상침수심에 대응하기 위한 홍수방어대책 수립이 필요하다. 본 연구는 방어침수심보다 아래에 위치한 건축물에 대해서 거실용도로 사용을 제한하는 수방기준을 마련했을 경우에 대하여 비용편익 분석을 통한 경제성 분석을 수행하고자 한다. 한편, 침수지역 및 침수심 산정을 위하여서는 GIS를 이용한 분석이 필요하며, GIS 분석을 통하여 산정된 결과를 바탕으로 건물피해액과 건물내용물에 대한 피해액을 산정할 수 있다. 적용과정에서 현재 서울시에서 구축되어진 데이터 구축 자료의 특성에 맞게 다차원법에서 제시한 일부 방법들을 수정하였다. 본 연구 결과 방어침수심의 설정을 통해 건축물에 대한 홍수피해를 방지하고, 주민의 보호 등을 포함한 침수피해방지대책 계획 수립에 이용할 수 있을 것으로 판단된다.

한국산 겨우살이(Viscum album coloratum)로부터 추출된 lectin의 닭에 대한 독성 및 뉴캐슬병 백신의 특이면역 증강 효과

여상건,Yeo, Sang-Geon 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.3

In order to search the availability of the lectin extracted from Korean mistletoe(Viscum album coloratum) as an adjuvant for the avian vaccines, attempts were made to determine toxicity of the lectin in chicks and its immunostimulating activity on the inactivated vaccines against Newcastle disease virus(NDV). For the determination of toxicity, the lectin was injected into the thigh muscle of SPF chicks(Charles River) of 1-week-old and observed hematologically and pathologically. For the determination of immunostimulating effects, lectin-adjuvanted, inactivated NDV vaccines were injected into the thigh muscle of SPF chicks in the same age group. Sera of the chicks were examined for the hemagglutination-inhibition(HI) antibodies induced, their HI titers and reaction to the NDV antigens. The data were further compared with those from aluminum hydroxide [$Al(OH)_3$]-adjuvanted vaccines and vaccines without adjuvant, and the results are as follows. There were no significant changes observed in the values of RBC, WBC, Hb, PCV, MCV, MCH, MCHC, AST, ALT, BUN, creatinine and total proteins in the chicks administered with lectin of 1.1, 2.2 and $22.2{\mu}g/kg$ body weight, which means the lectin has no effects on blood values and functions of liver and kidney. In histopathologic observation, no lesions were observed in the brain, heart, liver, lung, spleen, kidney, thymus and bursa of Fabricius of the chicks administered with lectin of 1.1, 2.2 and $22.2{\mu}g/kg$ body weight. There were inflammatory lesions, such as congestion, hemorrhage, edema, infiltration of macrophages and coagulation necrosis observed in the thigh muscle of chicks administered with lectin of $22.2{\mu}g/kg$ body weight, whereas no changes were observed in 1.1 and $22.2{\mu}g/kg$ lectin administered chicks. In chicks immunized with lectin($4.4{\mu}g/kg$ of body weight)-adjuvanted B1, LaSota and Ulster 2C vaccines, HI titers in reciprocal values for $log_2$ were 1.8-2.2 at 1 week after vaccination, which was similar with those of 1.5-2.9 by $Al(OH)_3$-adjuvanted vaccines. The HI titers by the lectin-adjuvanted vaccines reached to 3.9-5.3 at 4 weeks, whereas those by the $Al(OH)_3$-adjuvanted vaccines were more high as 7.3-9.3. Meanwhile, the immunostimulating effects of the lectin were recognized while compared to the HI titers with 2.4-3.7 in chicks immunized with vaccines without adjuvants at 4 weeks after vaccination. The chicks immunized with lectin-adjuvanted vaccines were enough to resist challenges by Kyojeongwon strain, a very virulent NDV at 4 weeks after vaccination as well as chicks immunized with $Al(OH)_3$-adjuvanted vaccines. The HI titers by the lectin-adjuvanted vaccines reached to high level as 8.7-10.3 as those with 8.2-9.6 by the $Al(OH)_3$-adjuvanted vaccines at 6 weeks after vaccination, which may be the booster effects by the challenge virus. Antibodies specific to the HN and F antigens of NDV were observed in the sera of both chicks immunized with lectin-adjuvanted vaccines and $Al(OH)_3$-adjuvanted vaccines.