A Hydrogel-based First-Aid Tissue Adhesive with Effective Hemostasis and Anti-bacteria for Trauma Emergency Management

Dongjie Zhang,Li Mei,Yuanping Hao,Bingcheng Yi,Jilin Hu,Danyang Wang,Yaodong Zhao,Zhe Wang,Hailin Huang,Yongzhi Xu,Xuyang Deng,Cong Li,Xuewei Li,Qihui Zhou,Yun Lu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Clinical tissue adhesives remain some critical drawbacks for managing emergency injuries, such as inadequate adhesive strength and insufficient anti-infection ability. Herein, a novel, self-healing, and antibacterial carboxymethyl chitosan/polyaldehyde dextran (CMCS/PD) hydrogel is designed as the first-aid tissue adhesive for effective trauma emergency management. Methods We examined the gel-forming time, porosity, self-healing, antibacterial properties, cytotoxicity, adhesive strength, and hemocompatibility. Liver hemorrhage, tail severance, and skin wound infection models of rats are constructed in vivo, respectively. Results Results demonstrate that the CMCS/PD hydrogel has the rapid gel-forming (~ 5 s), good self-healing, and effective antibacterial abilities, and could adhere to tissue firmly (adhesive strength of ~ 10 kPa and burst pressure of 327.5 mmHg) with excellent hemocompatibility and cytocompatibility. This suggests the great prospect of CMCS/ PD hydrogel in acting as a first-aid tissue adhesive for trauma emergency management. The CMCS/PD hydrogel is observed to not only achieve rapid hemostasis for curing liver hemorrhage and tail severance in comparison to commercial hemostatic gel (Surgiflo ®) but also exhibit superior anti-infection for treating acute skin trauma compared with clinical disinfectant gel (Prontosan ®). Conclusions Overall, the CMCS/PD hydrogel offers a promising candidate for first-aid tissue adhesives to manage the trauma emergency. Because of the rapid gel-forming time, it could also be applied as a liquid first-aid bandage for mini-invasive surgical treatment.

Biomechanical analysis of different experienced wearers during moderate high heel intensity locomotion

( Yan Zhang ),( Fekete Gusztav ),( Yaodong Gu ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1

Introduction: The high-heel design has been remaining one of the dominant features of women’s footwear. Social and fashion customs encourage the continued use of high-heel shoes (Hong et al., 2005) despite of detrimental effects on the musculoskeletal system, such as lower back pain, ankle sprains, foot pain, hallux valgus and increased predisposition toward degenerative knee osteoarthritis (Barkema, Derrick, & Martin, 2012; Chien et al., 2014; Dawson et al., 2002; Gu et al., 2014). The purpose of this study was to clarify differences in lower limb kinematics and GRF between EW and IEW during moderate high-heel jogging and running. It was hypothesized that EW would show faster self-select speeds of jogging and running than IEW; EW would decrease joint instability while increase GRF in comparison with IEW; changes of lower limb joints (range of motions and peak angles) and GRF would increase as speed increased for all wearers. Method: Eleven experienced female wearers of moderate high-heel shoes (EW: age: 24.2±1.2 years; height: 160±2.2 cm; mass: 51.6±2.6 kg) and eleven matched controls (IEW; age: 23.7±1.3 years; height: 162.3±2.3 cm; mass: 52.6±4.5 kg) participated in this test with informed written consent, as approved by the Ethics Committee of Ningbo University. Subjects completed jogging and running tasks separately at self-selected speed along a 10-meter-walkway. A force platform (Kistler, Switzerland) was fixed in the middle of the walkway and utilized to collect GRF at the frequency of 1000 Hz. The 8-camera Vicon motion analysis system (Oxford Metrics Ltd., Oxford, UK) was used to capture lower limb kinematics at the frequency of 200 Hz. Subjects were required to wear tight-fitting pants and 16 reflective markers (diameter: 14 mm) were attached with adhesive on the left and right lower limbs, respectively. Result: Fig1, 2 summarize the main GRF and VALR characteristics of EW and IEW. Impact force (Fz1) showed no significant differences between wearing experience or speeds. The maximal vertical GRF (Fz2) of EW during jogging was significantly larger than that of IEW jogging. EW during running showed significantly larger Fz2 compared with IEW jogging and running. Discussion: A key finding of this study was that compared with IEW, EW showed reduced joint ROM during stance phase to prevent excessive joint loading except for motions of knee and hip in sagittal plane that respectively aid in propulsion and load attenuation instead. Moderate high-heel shoes placed IEW at a greater risk of joint and soft tissue injury with generally larger peak angles during stance phase. However, the effect of these conservative control strategies adopted by EW partially lost during running in comparison with jogging. As speed increased from jogging to running, EW mainly relied on increasing SL, leading to landing with narrow high heel, which consequently resulted in an extremely high loading rate. From a kinetic perspective, EW also tended to have bone-on-bone injury in high-heeled running. In conclusion, moderate high-heeled wearers who have to run, whether regularly or occasionally, are putting themselves at high risk of lower limb damage. If having to run on high heels, even moderate ones, it is crucial for wearers to control joint stability and strike pattern consciously. Reference Barkema, D. D., Derrick, T. R., & Martin, P. E. (2012). Heel height affects lower extremity frontal plane joint moments during walking. Gait & posture, 35(3), 483-488. Chien, H. L., Lu, T. W., & Liu, M. W. (2014). Effects of long-term wearing of high-heeled shoes on the control of the body``s center of mass motion in relation to the center of pressure during walking. Gait & posture, 39(4), 1045-1050. Dawson, J., Thorogood, M., Marks, S. A., Juszczak, E., Dodd, C., Lavis, G., & Fitzpatrick, R. (2002). The prevalence of foot problems in older women: a cause for concern. Journal of Public Health, 24(2), 77-84. Gu, Y., Li, F., Li, J., Feng, N., Lake, M. J., Li, Z., & Ren, J. (2014). Plantar Pressure Distribution Character In Young Female With Mild Hallux Valgus Wearing High-heeled Shoes. Journal of Mechanics in Medicine and Biology, 14(01), 1450008. Hong, W.-H., Lee, Y.-H., Chen, H.-C., Pei, Y.-C., & Wu, C.-Y. (2005). Influence of heel height and shoe insert on comfort perception and biomechanical performance of young female adults during walking. Foot & Ankle International, 26(12), 1042-1048.

PFP@PLGA/Cu12Sb4S13-mediated PTT ablates hepatocellular carcinoma by inhibiting the RAS/MAPK/MT-CO1 signaling pathway

Dong Tianxiu,Jiang Jian,Zhang Hao,Liu Hongyuan,Zou Xiaomeng,Niu Jiamei,Mao Yingxuan,Zhu Mingwei,Chen Xi,Li Zizhuo,Chen Yaodong,Shi Chunying,Yang Xiuhua 나노기술연구협의회 2021 Nano Convergence Vol.8 No.29

Hepatocellular carcinoma (HCC) is one of the most malignant tumors in the world, and patients with HCC face a poor prognosis. The conventional therapeutic strategies for HCC have undergone a challenge-riddled evolution owing to side effects and unsatisfactory efficacy. Here, aiming to provide a new method of HCC elimination, we formulated a novel multifunctional nanocapsule (PFP@PLGA/Cu 12 Sb 4 S 13 , PPCu) with applications in contrast-enhanced ultrasound imaging (CEUS) and photothermal therapy (PTT). These PPCu were successfully constructed with an average diameter of 346 nm (polydispersity index, PDI = 0.276). The reinforced contrast ratio of these PPCu was determined by CEUS, revealing their promising applications in image-guided monitoring of HCC treatment. Furthermore, the excellent photoabsorption and biocompatibility indicated by organ H&E staining indicated that PPCu meet quality expectations for use as photothermal transduction agent (PTA). PPCu treatment at 50 °C and higher temperatures efficiently repressed the proliferation, induced the apoptosis and decreased the motility of HCC cells. These effects might have been results of RAS/MAPK/MT-CO1 signaling pathway inhibition. In summary, PPCu were constructed to integrate CEUS and PTT successfully into therapy, which can lead to HCC elimination through RAS/MAPK/MT-CO1 signaling pathway repression.

Research Progress on Non-coding RNAs in Cholesteatoma of the Middle Ear

Dongliang Liu,Hang Zhang,Xiulan Ma,Yaodong Dong 대한이비인후과학회 2023 Clinical and Experimental Otorhinolaryngology Vol.16 No.2

Cholesteatoma of the middle ear is a common disease in otolaryngology that is receiving increasing attention. It is estimat-ed that over five million people around the world have suffered from middle ear cholesteatoma. The annual incidence ofmiddle ear cholesteatoma has been reported to be 9.2 per 100,000 in adults and 3 per 100,000 in children. Without timelydiscovery and intervention, cholesteatomas can become perilously large and damage intratemporal structures, causing vari-ous intracranial and extracranial complications. No practical nonsurgical treatments are currently available. Although multi-ple hypotheses exist, research directions have consistently focused on cell proliferation, apoptosis, and bone destruction. Non-coding RNAs (ncRNAs), especially microRNAs (miRNAs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs),have recently received increasing attention because of their key roles in gene expression, cell cycle regulation, and the de-velopment of many diseases. Although ncRNAs are not involved in protein translation, they are abundant in the genome,with only approximately 2% of genes encoding proteins and the remaining approximately 98% encoding ncRNAs. Thepurpose of this review is to summarize the current state of knowledge regarding the specific role of ncRNAs in middle earcholesteatoma.

Investigation on Pore-fracture of Coal and Its Influence Mechanism on Uniaxial Compression Failure Behavior

Yutao Li,Qingwei Guo,Yaodong Jiang,Bo Zhang,Xuehua Li 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.5

Pore-fracture is an important component of coal, affecting the uniaxial compression failure behavior. However, it is difficult to effectively characterize the influence mechanism because of the complexity and randomness of components in coal. In this paper, scanning electron microscope (SEM), computed tomography (CT), indentation hardness and uniaxial compressiontests are conducted for specimens with high, medium and no bursting proneness to investigate it. The results indicate that distribution of three components (matrix, minal and pore-fracture) in specimens are extremely different, which is the embodiment of tectonic structure activity and geological condition of mineralization. The levels of bursting proneness of coal, the tectonic structure features, tectonic structure activities and external functions are related. 3-D reconstruction on the basis of CT images can visualize internal complex structure of specimens. The numerical simulation results demonstrate that distribution of pore-fracture, mechanical properties of matrix and minal are significant inducement of specimen’s failure difference in the identical loading mode. Main failure mode of specimens with high, medium and no bursting proneness are different, which proves that main failure mode of specimen correlates with its mechanical properties. The mechanical failure behavior of specimen under uniaxial compression is influenced by loading mode, mechanical properties of matrix and minal, pore-fracture feature and other considerations. The loading mode is extrinsic cause of mechanical failure behavior of specimens, also an inducing factor and could be intervened. Furthermore, mechanical properties of matrix and minal determine bearing capacity and ultimate failure mode of specimens. Primary pore-fracture seriously affects extension process and extension mode of secondary pore-fracture, and the internal damage development of specimens. Notably, coal composed by the convergence of matrix, minal and pore-fracture, all these are internal cause of mechanical failure behavior of specimens, which belong to basic factors and hard to be intervened.

Comparative study of the sublethal effects of indoxacarb on juvenile hormone titer and juvenile hormone esterase mRNA expression of Nilaparvata lugens and Chilo suppressalis in rice

Jing-Lan Liu,Zhenghui Li,Hong-Mei Zhang,Haitao Du,Xu Ding,Yaodong Zhou,Jin-Cai Wu,AndrewWong 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Both the rice stem borer, Chilo suppressalis, and the brown planthopper, Nilaparvata lugens are important rice pests. The study is a comparative analysis on the effects of indoxacarb on the pest's juvenile hormone (JH) titer and juvenile hormone esterase (JHE) mRNA expression. RT-PCR and RACE were used to amplify the full length Csjhe (JHE of C. suppressalis) cDNA fromC. suppressalis. The full length cDNA is 1841 bp in length and contains a 1734 bp open reading encoding 577 amino acid residues. An amino acid sequence alignment shows that Csjhe shares high identity with other three other lepidopterans homologues including Manduca sexta, Bombyx mori, and Choristoneura fumiferana. JH III titer in 4th instar larvae of C. suppressalis was significantly reduced with 0.1, 0.2, 0.4 and 0.8 g/L indoxacarb, which were decreased by 11.0%, 22.3%, 32.0% and 37.7% respectively, the expression levels of Csjhe mRNA were 1.38, 1.92 and 5.25 times the control levels in 4th instar larvae with 0.2, 0.4 and 0.8 g/L indoxacarb. Similarly, all of the tested concentrations of indoxacarb caused a significant decrease in JH III titer of N. lugens adult females after indoxacarb treatments, Nljhe (JHE of N. lugens)mRNAaverage expression levels were 4.8, 5.0 and 4.4 times before mating and 6.5, 7.6 and 5.3 times after mating compared to the control at 1, 3 and 5 days, respectively. The comparative studies of the effects of indoxacarb on JH III titer and JHE mRNA further have a significance to a guided integrated pest management.

Optimal Consensus Control for Heterogeneous Nonlinear Multiagent Systems with Partially Unknown Dynamics

Tao Wang,Hao Fu,Jinbin Li,Yaodong Zhang,Xinfeng Zhou,Xin Chen 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.9

This paper focuses on an optimal consensus problem for heterogeneous discrete-time nonlinear multiagent systems (MASs) with partially unknown dynamics. For those systems, it is difficult to obtain the solution of the coupled Hamilton-Jacobi-Bellman (HJB) equations, which is necessary to address the optimal consensus problem. A new hierarchical and distributed optimal control strategy is developed to derive the near solution of the HJB equations. Its control structure consists of the model reference adaptive control (MRAC) layer and distributed control layer. In the MRAC layer, the adaptive feedforward and feedback controller is designed to make the states of followers converge to ones of their corresponding reference models. Then, the optimal consensus problem of heterogeneous MASs is formulated as that of homogeneous MASs. In the distributed control layer, an online distributed value iteration algorithm is proposed to approximate the optimal solution of the HJB equations for reference models. Thereby, the optimal consensus is also achieved for the heterogeneous MASs. The two convergence properties are analyzed to demonstrate the MRAC performance and the optimal consensus, respectively. Simulation results verify the effectiveness of the proposed strategy.

Sequential Prediction of the TBM Tunnelling Attitude Based on Long-Short Term Memory with Mechanical Movement Principle

Ruirui Wang,Yuhang Xiao,Qian Guo,Hai Wang,Lingli Zhang,Yaodong Ni 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

TBM tunnelling attitude controlling is a significant issue for guaranteeing the tunnel fitting the expected tunnel axis, with directly influence the tunnel quality. The key to solve the problem is to establish the relationship between the tunnelling attitude and the controlling parameters and to predict the tunnelling attitude accordingly. For this, this paper introduced a TBM tunnelling attitude predicting method. In detail, using Long-Short Term Memory (LSTM), the initial tunnelling attitude and the controlling parameters of each later ring are taken as input, while the tunnelling attitude of each later rings are regarded as the output, and the relationship between the input and output is established. Meanwhile, for avoid the over-fitting and error accumulation risk of LSTM, the theoretical relationship between the input and output is also built based on the TBM mechanical movement principle, and it is also involved into the LSTM-based relationship as constraints. The proposed method is verified by the field data collected from the 6th Section of the Qingdao Metro Project, and the results reveal that the proposed LSTM-based method is accurate and acceptable.