Evaluation of the close degree of the spatial relationship between natural enemies of tea gardens and Frankliniella intonsa based on variance analysis of cluster samples

Honghao Cheng,Shiyan Chen,Xiaomeng Wu,Yue Xu,Lin Zhang,Jiazhao Sun,Xiazhi Zhou,Yunding Zou,Shoudong Bi 한국곤충학회 2022 Entomological Research Vol.52 No.6

The aim of this paper is to clarify the difference of spatial closeness between natural enemies and Frankliniella intonsa, and to provide a scientific basis for rational protection and utilization of natural enemies. This paper applied the block-sample square difference analysis method, Gray correlation degree method, the aggregation intensity index method and ρ index method to study the difference in the closeness of the spatial relationship between F. intonsa and natural enemies when the number of F. intonsa and their natural enemies was the highest and the minimum area occupied by F. i n t o n s a individuals and colonies in five tea plantations in Hefei, Anhui province in 2021 and four tea plantations in 2020. The results were as follows: 1) Tetragnatha squamata Karsch, Xysticus ephippiafus, Erigonidium graminicolum and Theridion Octomaculatum were the top four natural enemies closely related to F. intonsa in 2021. The top three natural enemies closely related to F. intonsa in 2020 were Tetragnatha squamata Karsch, Xysticus ephippiafus and Theridion Octomaculatum. Tw o y e a r s a g o , Tetragnatha squamata Karsch and Xysticus ephippiafus were the same. 2) Cluster size did not change the distribution patterns of natural enemies and F. intonsa. 3) The aggregation of F. intonsa was caused by their own causes or some environmental factors, and the aggregation of natural enemies was caused by environmental factors. 4) In 2021, the minimum area occupied by individuals and groups of F. intonsa in tea gardens was 2m2, and in 2020, it was 8 m2.

Comprehensive evaluation of natural enemy dominant species of Breuipalpus oboyats in tea garden in autumn and winter

Zhang Lin,Sun Jiazhao,Wu Xiaomeng,Xu Yu,Bi Shoudong,Zou Yunding 한국곤충학회 2021 Entomological Research Vol.51 No.12

The purpose of this study was to comprehensively evaluate the dominant natural enemies of Breuipalpus oboyats in autumn and winter tea gardens. The relationship between natural enemies and B.oboyats in time and space was studied by niche analysis and geostatistics combined with angular cosine coefficient method. According to the comparison of the sum of closeness index, it is found that the top three natural enemies (from large to small) that are most closely related to B.oboyats in the “Nongkangzao” tea garden are Plexippus paykulli, Clubiona japonicola,and Xysticus ephippiafus; “Pingyangtezao” tea garden are X.ephippiafus, Plexippus setipet,andOxyopes sertatus. In front of the two tea gardens, one of the three natural enemies is the same. The evaluation of the comprehensive closeness index of the two tea gardens shows that the top three natural enemies most closely related to B.oboyats are X.ephippiafus, P.paykulli, and O.sertatus. X.ephippiafus is the most important natural enemy of B.oboyats in tea gardens in autumn and winter; the dominant natural enemies of the same pests in different varieties of tea gardens in the same area are often different.

Differences in the closeness of spatial relationship between Ricanidae in their prime and natural enemies in five kinds of tea gardens

Cheng Xian,Zhang Lin,Wu Xiaomeng,Xu Yue,Sun Jiazhao,Zhou Xiazhi,Zou Yunding,Bi Shoudong 한국곤충학회 2022 Entomological Research Vol.52 No.8

To identify the natural enemy species which are close to Ricanidae in spatial relationship and to provide scientific basis for biological control and reasonable protection of natural enemies, geostatistics and the angular cosine coefficient method were used to analyze the population of Ricanidae in their prime and their natural enemies in the Anji white tea garden, the Longjing43 tea garden, the Nongkangzao tea garden, the Pingyangtezao tea garden and the Wuniuzao tea garden. The spatial relationship between six natural enemies and Ricanidae was also studied. The angular cosine coefficients were normalized to obtain the intimacy index. According to the sum of the intimacy index and the serial number of the intimacy index of each natural enemy in five tea gardens, the following conclusions could be drawn: the top three natural enemies closely related to Ricanidae in spatial relationship were Clubiona reichlini, Clubiona japonicola and Misumenops tricuspidatus; at least two species of the top three natural enemies in each tea garden were the same as the top three natural enemies in the comprehensive analysis of the five tea gardens; one of the factors that determined the spatial relationship between natural enemies and Ricanidae was the ratio of the number of Ricanidae and natural enemies. The results of this study identified the spider species of natural enemies which should be rationally used and protected in the five tea gardens.

Analysis of the following effect of the natural enemies with Frankliniella intonsa in tea garden

Cheng Honghao,Chen Shiyan,Wu Xiaomeng,Xu Yue,Zhang Lin,Sun Jiazhao,Zhou Xiazhi,Zou Yunding,Bi Shoudong 한국곤충학회 2022 Entomological Research Vol.52 No.8

In order to reasonably protect and utilize natural enemies for comprehensive control of Frankliniella intonsa, a systematic investigation was conducted on F. i n t o n s a and natural enemies in tea gardens in Hefei, Anhui Province, and the spatial following effect of natural enemies on F. intonsa was studied. The semi-variogram of natural enemies and thrips was obtained by the geostatistics method, and the correlation degree between them was analyzed by the grey correlation degree analysis method. The higher the correlation degree between natural enemies and F. intonsa, the closer the relationship between natural enemies and F. intonsa was. In this study, we analyzed the relationship between 11 natural enemies of F. i n t o n s a in tea gardens from October 28, 2020 to November 20, 2020 and from August 22, 2021 to November 19, 2021. The top five natural enemies with the largest closeness index to F. intonsa in Huangshan Dayezhong tea garden are Oxyopes sertatus, Theridion octomaculatum, Plexippus setipes, Xysticus ephippiafus and Erigonidium graminicolum. The top five natural enemies with the largest closeness index to F. intonsa in Pingyang Tezao tea garden are Erigonidium graminicolum, Plexippus setipes, Xysticus ephippiafus, Oxyopes sertatus and Clubiona japonicola.Among the top five natural enemies in the two tea gardens, those in common are Oxyopes sertatus, Erigonidium graminicolum, Xysticus ephippiafus and Plexippus setipes. According to the summation of the closeness index and the sum of the serial numbers, the top five natural enemies closely related to the spatial following of F. intonsa in the tea gardens were Oxyopes sertatus, Plexippus setipes, Erigonidium graminicolum, Xysticus ephippiafus and Theridion octomaculatum. One of the factors closely related to the space of F. i n t o n s a in the same tea garden was the ratio of F. i n t o n s a to a certain natural enemy. The smaller the ratio was, the closer the following relationship was. The results of this study provide scientific basis for biological control and natural enemy protection of F. intonsa.

PFP@PLGA/Cu12Sb4S13-mediated PTT ablates hepatocellular carcinoma by inhibiting the RAS/MAPK/MT-CO1 signaling pathway

Dong Tianxiu,Jiang Jian,Zhang Hao,Liu Hongyuan,Zou Xiaomeng,Niu Jiamei,Mao Yingxuan,Zhu Mingwei,Chen Xi,Li Zizhuo,Chen Yaodong,Shi Chunying,Yang Xiuhua 나노기술연구협의회 2021 Nano Convergence Vol.8 No.29

Hepatocellular carcinoma (HCC) is one of the most malignant tumors in the world, and patients with HCC face a poor prognosis. The conventional therapeutic strategies for HCC have undergone a challenge-riddled evolution owing to side effects and unsatisfactory efficacy. Here, aiming to provide a new method of HCC elimination, we formulated a novel multifunctional nanocapsule (PFP@PLGA/Cu 12 Sb 4 S 13 , PPCu) with applications in contrast-enhanced ultrasound imaging (CEUS) and photothermal therapy (PTT). These PPCu were successfully constructed with an average diameter of 346 nm (polydispersity index, PDI = 0.276). The reinforced contrast ratio of these PPCu was determined by CEUS, revealing their promising applications in image-guided monitoring of HCC treatment. Furthermore, the excellent photoabsorption and biocompatibility indicated by organ H&E staining indicated that PPCu meet quality expectations for use as photothermal transduction agent (PTA). PPCu treatment at 50 °C and higher temperatures efficiently repressed the proliferation, induced the apoptosis and decreased the motility of HCC cells. These effects might have been results of RAS/MAPK/MT-CO1 signaling pathway inhibition. In summary, PPCu were constructed to integrate CEUS and PTT successfully into therapy, which can lead to HCC elimination through RAS/MAPK/MT-CO1 signaling pathway repression.