Benzochloroporphyrin Derivative Induced Cytotoxicity and Inhibition of Tumor Recurrence During Photodynamic Therapy for Osteosarcoma

Gong, Hai-Yang,Sun, Meng-Xiong,Hu, Shuo,Tao, Ying-Ying,Gao, Bo,Li, Guo-Dong,Cai, Zheng-Dong,Yao, Jian-Zhong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Photodynamic therapy (PDT) is a promising cancer treatment modality that uses dye-sensitized photooxidation of biologic matter in target tissue. This study explored effects of the photosensitizer BCPD-17 during PDT for osteosarcoma. LM-8 osteosarcoma cells were treated with BCPD-17 and cell viability after laser irradiation was assessed in vitro with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of BCPD-17 during PDT recurrence were then examined on tumor-bearing mice in vivo. BCPD-17 had dosedependent cytotoxic effects on LM-8 osteosarcoma cells after laser irradiation which also had energy-dependent effects on the cells. The rate of local recurrence was reduced when marginal resection of mice tumors was followed by BCPD-17-mediated PDT. Our results indicated BCPD-17-mediated PDT in combination with marginal resection of tumors is a potentially new effective treatment for osteosarcoma.

Expression of Cholera Toxin B Subunit and Assembly as Functional Oligomers in Silkworm

Gong, Zhao-Hui,Jin, Hui-Qing,Jin, Yong-Feng,Zhang, Yao-Zhou Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6

The nontoxic B subunit of cholera toxin (CTB) can significantly increase the ability of proteins to induce immunological tolerance after oral administration, when it was conjugated to various proteins. Recombinant CTB offers great potential for treatment of autoimmune disease. Here we firstly investigated the feasibility of silkworm baculovirus expression vector system for the cost-effective production of CTB under the control of a strong polyhedrin promoter. Higher expression was achieved via introducing the partial non-coding and coding sequences (ATAAAT and ATGCCGAAT) of polyhedrin to the 5' end of the native CTB gene, with the maximal accumulation being approximately 54.4 mg/L of hemolymph. The silkworm bioreactor produced this protein vaccine as the glycoslated pentameric form, which retained the GM1-ganglioside binding affinity and the native antigenicity of CTB. Further studies revealed that mixing with silkworm-derived CTB increases the tolerogenic potential of insulin. In the nonconjugated form, an insulin : CTB ratio of 100 : 1 was optimal for the prominent reduction in pancreatic islet inflammation. The data presented here demonstrate that the silkworm bioreactor is an ideal production and delivery system for an oral protein vaccine designed to develop immunological tolerance against autoimmune diabetes and CTB functions as an effective mucosal adjuvant for oral tolerance induction.

Vector Control of Stator-Permanent Magnet Memory Machine Based on Three Magnetization State Manipulations

Gong-De Yang,Ming-Yao Lin,Nian Li,Li Hao 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.1

Owing to the estimated permanent magnet (PM) flux linkage fluctuation, the stator-PM memory machine employing the continuous magnetization state (MS) manipulation may suffer from frequent MS manipulations and the accidental inverter damage in its extended speed range. In order to deal with this issue, in this paper, a novel vector control based on three MS manipulations is proposed and implemented on the existing hybrid PM axial field flux-switching memory machine. After a brief analysis of the researched machine configuration, the principle of the three MS manipulations, the remedial sliding mode speed regulator and the current distribution strategy are discussed. The simulated results show that the proposed scheme reduces the speed and electromagnetic torque overshoot and improves the dynamic performance. Meanwhile, compared with the flux-weakening control, the wide speed range can be obtained. Finally, the experiment is carried out to verify the validity of the theory.

Experimental study on micro-grinding force and subsurface microstructure of nickel-based single crystal superalloy in micro grinding

Yadong Gong,Yunguang Zhou,Xuelong Wen,Jun Cheng,Yao Sun,Lianjie Ma 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.7

Nickel-based single crystal superalloy has no grain boundary, which leads to the removal mechanism difference between single crystal material and polycrystalline material. Firstly, the removal mechanism of grinding nickel-based single crystal superalloy is analysed. Then, the developed prediction model of the tangential force F t and the normal force F n are established. Forthermore, the impacts of grinding parameters on grinding force and microstructure of grinding surface and sub-surface are analysed. Finally, some measurements to reduce or prevent recrystallization are proposed. As a result, the most shear slipping planes of nickel-based single crystal superalloy are {111} planes; with the increasing of the feeding rate and grinding depth, the micro-grinding force and the thickness of subsurface plastic deformation increase; the micro-grinding force and the thickness of subsurface plastic deformation decrease with the increasing of spindle speed. These results have some theoretical and engineering significance to the production of single crystal material parts.

Simulation Research on the Thermal Effects in Dipolar Illuminated Lithography

Changcheng Yao,Yan Gong 한국광학회 2016 Current Optics and Photonics Vol.20 No.2

The prediction of thermal effects in lithography projection objective plays a significant role in thereal-time dynamic compensation of thermal aberrations. For the illuminated lithography projection objective,this paper applies finite element analysis to get the temperature distribution, surface deformation and stressdata. To improve the efficiency, a temperature distribution function model is proposed to use for thesimulation of thermal aberrations with the help of optical analysis software CODE V. SigFit is approvedintegrated optomechanical analysis software with the feature of calculating OPD effects due to temperaturechange, and it is utilized to prove the validation of the temperature distribution function. Results showthat the impact of surface deformation and stress is negligible compared with the refractive index change;astigmatisms and 4-foil aberrations dominate in the thermal aberration, about 1.7 λ and 0.45 λ. The systemtakes about one hour to reach thermal equilibrium and the contrast of the imaging of dense lines get worseas time goes on.

Selective flotation of brucite from calcite using HEDP-4Na as an inhibitor in a SDS system

Xiufeng Gong,Jin Yao,Bin Yang,Yafeng Fu,Yulian Wang,Wanzhong Yin 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

In this study, the selective adsorption of (1-hydroxyethylidene) bis-phosphonic acid tetrasodium salt(HEDP-4Na) on a calcite surface and its impact on the separation of brucite were examined using amicroflotation test, contact angle measurement, zeta potential measurement, X-ray photoelectron spectroscopy(XPS), and atomic force microscopy (AFM). The microflotation test revealed that HEDP-4Naselectively inhibited calcite from brucite at pH 10.5 in the sodium dodecyl sulfonate (SDS) system. Under the dosage condition of 180-mg/L HEDP-4Na, a single-mineral floating difference of 73.79% wasachieved between brucite and calcite. The contact angle measurement revealed that HEDP-4Na considerablyreduced the contact angle of calcite in the SDS system but only weakly affected the contact angleof brucite. Zeta potential analysis and Fourier transform infrared spectroscopy demonstrated that theHEDP-4Na adsorption interfered with the SDS adsorption on the surface of calcite; however, theHEDP-4Na adsorption had little effect on the SDS adsorption on the surface of brucite. XPS and AFM analysesdemonstrated that the selectivity and effect of HEDP-4Na on calcite were considerably stronger thanthose on brucite and the HEDP-4Na adsorption on the calcite surface was significant and strong.

Hypersonic Aero-Heating Ground-Test Simulation Technique

Li, Ruiqu,Yao, Dapeng,Sha, Xinguo,Gong, Jian The Society for Aerospace System Engineering 2015 International Journal of Aerospace System Engineer Vol.2 No.2

It would encounter some complicated flow fields, such as transition, separation, reattachment and disturbances, in the hypersonic flight. Thus, it is difficult to theoretically analyze the hypersonic aerothermodynamics effects, so that the ground-test simulation is thought of as one of the most important methods to improve the understanding level of the hypersonic aerothermodynamics. However, the aero-heating tests could not simulate all aerodynamics and geometry parameters in the real flight due to the differences between the experimental environments supplied by the ground facilities and the flight, so that the feasible technique for the ground-test simulation of the hypersonic aerothermodynamics effects is required to be advanced. The key parameters that are especially required to simulate for aero-heating tests are analyzed and one detailed approach is suggested to perform the experimental investigation on the hypersonic aero-heating effects in the ground facilities in this paper, and the tests are performed in the FD-20 gun tunnel of CAAA (China Academy of Aerospace Aerodynamics) to give out the data which could be used to confirm the equation from the theoretical analysis.

Nuciferine modulates the gut microbiota and prevents obesity in high-fat diet-fed rats

Wang Yueping,Yao Weifan,Li Bo,Qian Shiyun,Wei Binbin,Gong Shiqiang,Wang Jing,Liu Mingyan,Wei Minjie 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Gut microbiota dysbiosis has a significant role in the pathogenesis of metabolic diseases, including obesity. Nuciferine (NUC) is a main bioactive component in the lotus leaf that has been used as food in China since ancient times. Here, we examined whether the anti-obesity effects of NUC are related to modulations in the gut microbiota. Using an obese rat model fed a HFD for 8 weeks, we show that NUC supplementation of HFD rats prevents weight gain, reduces fat accumulation, and ameliorates lipid metabolic disorders. Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that NUC changed the diversity and composition of the gut microbiota in HFD-fed rats. In particular, NUC decreased the ratio of the phyla Firmicutes/Bacteroidetes, the relative abundance of the LPS-producing genus Desulfovibrio and bacteria involved in lipid metabolism, whereas it increased the relative abundance of SCFA-producing bacteria in HFD-fed rats. Predicted functional analysis of microbial communities showed that NUC modified genes involved in LPS biosynthesis and lipid metabolism. In addition, serum metabolomics analysis revealed that NUC effectively improved HFD-induced disorders of endogenous metabolism, especially lipid metabolism. Notably, NUC promoted SCFA production and enhanced intestinal integrity, leading to lower blood endotoxemia to reduce inflammation in HFD-fed rats. Together, the anti-obesity effects of NUC may be related to modulations in the composition and potential function of gut microbiota, improvement in intestinal barrier integrity and prevention of chronic low-grade inflammation. This research may provide support for the application of NUC in the prevention and treatment of obesity.

Effect of microstructures on the shrinkage of injection molding product

Yue Wu,Yao Gong,차경제,박장민 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.3

Shrinkage of injection molding product is a well-known phenomenon which is determined by many factors such as material property, processing condition, mold design, and product design. This study aims to investigate the effect of microscopic structures on the shrinkage of macroscopic product. Flat specimens with and without microstructures were injection molded and the distribution of in-plane shrinkage was measured using an optical method. In the case without microstructures, anisotropic shrinkage is observed and the shrinkage magnitude increases with the distance from the gate. By applying the microstructures, the shrinkage could be reduced significantly, and the effect is especially noticeable when lower packing pressure was applied in the injection molding process. The numerical simulation result is also presented which suggests the importance of material data and lateral motion modeling for accurate prediction of the shrinkage.

Expression of Cholera Toxin B Subunit and Assembly as Functional Oligomers in Silkworm

Zhao Hui Gong,Hui Qing Jin,Yong Feng Jin,Yao Zhou Zhang 한국생활환경학회 2005 BMB Reports Vol.38 No.6