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Yangmi Kim, Kyung Sun Park 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.2
Bladder cancer is a common cancer in smoking men and may correlate with mechanosensitive potassium channels because the urinary bladder is a stretch sensing organ. Two-pore K+ channels (K2P), such as TASK3 and TREK1, have recently been shown to play a critical role in both cell apoptosis and tumorigenesis. Of the channels, TREK1 can be activated by many physiological stimuli, including polyunsaturated fatty acids, and intracellular pH, hypoxia, and neurotransmitters. Here we attempted to determine whether TREK1 is functionally expressed in bladder cancer 253J cells. K2P channels, including TREK1, TREK2, TASK1, TASK3, and TWIK1, were quantified in cultured human bladder cancer 253J cells using real time quantitative RT-PCR (qRT-PCR) analysis. Among them, TREK1-like channel was recorded at a single channel level using the patch-clamp technique. The TREKl-like channel, with single-channel conductance of ~90 pS at −80 mV, was recorded in symmetrical 150 mM KCl using an excised inside-out patch configuration. The current-voltage relationships were linear and were insensitive to tetraethylammonium. The channel was activated by membrane stretch, free fatty acids, and intracellular acidosis. These results with electrophysiological properties resemble to those of K2P channel, for instance, TREK1. Therefore, we conclude that TREK1 channel is functionally present in bladder cancer 253J cells.
The TREK2 Channel Is Involved in the Proliferation of 253J Cell, a Human Bladder Carcinoma Cell
Park, Kyung-Sun,Han, Min Ho,Jang, Hee Kyung,Kim, Kyung-A,Cha, Eun-Jong,Kim, Wun-Jae,Choi, Yung Hyun,Kim, Yangmi The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.6
Bladder cancer is the seventh most common cancer in men that smoke, and the incidence of disease increases with age. The mechanism of occurrence has not yet been established. Potassium channels have been linked with cell proliferation. Some two-pore domain $K^+$ channels (K2P), such as TASK3 and TREK1, have recently been shown to be overexpressed in cancer cells. Here we focused on the relationship between cell growth and the mechanosensitive K2P channel, TREK2, in the human bladder cancer cell line, 253J. We confirmed that TREK2 was expressed in bladder cancer cell lines by Western blot and quantitative real-time PCR. Using the patch-clamp technique, the mechanosensitive TREK2 channel was recorded in the presence of symmetrical 150 mM KCl solutions. In 253J cells, the TREK2 channel was activated by polyunsaturated fatty acids, intracellular acidosis at -60 mV and mechanical stretch at -40 mV or 40 mV. Furthermore, small interfering RNA (siRNA)-mediated TREK2 knockdown resulted in a slight depolarization from $-19.9mV{\pm}0.8$ (n=116) to $-8.5mV{\pm}1.4$ (n=74) and decreased proliferation of 253J cells, compared to negative control siRNA. 253J cells treated with TREK2 siRNA showed a significant increase in the expression of cell cycle boundary proteins p21 and p53 and also a remarkable decrease in protein expression of cyclins D1 and D3. Taken together, the TREK2 channel is present in bladder cancer cell lines and may, at least in part, contribute to cell cycle-dependent growth.
발도르프 유치원 음악교육을 적용한 교회 유치부 교육활동에 대한 고찰
박지연,최양미 안양대학교 2005 人文科學硏究 Vol.13 No.-
The purpose of this study is to search the possibility to apply the music education of Waldorf kindergarten to the church preschool educational activities. The method of this study involved the analyzing of books, articles and related materials about the idea and philosophy of Steiner and Waldorf kindergarten, early childhood music education, Korean church preschool education child. Steiner insisted on the human formation through music education using Pentatonic, music education which emphasized rhythm, music education according to the development course. In Waldorf kindergarten which is based on the educational idea of Steiner, they are proceeding the music education through instruments(Ryer) and 5 note scales, especially while comprising rhythm activity variously such as Rygen, Eurythmie among daily schedule to practice the idea of Steiner who attached importance in rhythm. Now, it is lacking in music education element in church preschool educational activities, so in this viewpoint, if we apply the music education of Waldorf kindergarten to the church preschooler worship and education activities, it will be very effective. Especially, if we apply the integral music education of Waldorf kindergarten that attaches importance to the rhythm in all educational activities, using eurythmie, instruments and 5 note scales, it may be rhythmic worship and activities. But for the music education of Waldorf kindergarten to be newly applied to church preschool education activities, the challenging and researching attitude by religious workers and teachers are necessary, and continuous effort is needed to rightly understand the idea of Steiner and apply it to our situation. Also, the attempt to apply music integrally into every activities, and rhythm activity utilizing eurythmie and education activity utilizing instruments and 5 note scales must be activated, and when these efforts are made continuously, music education may settle newly in Korean churches' preschool group, and the preschoolers will like music through music and will grow up as the preschool children who are familiar with music, and they will grow up to personally matured humans.
Kyoung Sun Park,Chan Hyung Kim,Eun-Young Shin,Yangmi Kim 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.4
Two-pore domain background K⁺ channels are widely believed to play an important role in maintaining and regulating neuronal excitability. We studied whether riluzole-sensitive background K⁺ channels are functionally expressed in dorsal root ganglion (DRG). DRG neurons were dissociated from 1 day rats and electrophysiological recording was performed using a patch-clamp amplifier. Inside-out patches and cellattached membrane patches were voltage clamped at a constant potential (mainly -60 mV) in symmetrical 150 mM K⁺ solutions. The recorded K⁺ channels in DRG neurons was determined to be TRAAK, as judged by its electrophysiological bursting kinetics and activation by membrane stretch, free fatty acids, intracellular alkalosis and riluzole. These data suggest that DRG neurons possess a riluzole sensitive background current which shares several pharmacological and electrophysiological properties with recently cloned two-pore K⁺ channels, particularly TRAAK.
Kyoung Sun Park,Kyung Hwa Jung,Young Gyu Chai,Chan Hyung Kim,Eun-Young Shin,Yangmi Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.3
Recently, we demonstrated that large conductance Ca²⁺-activated K⁺ channel (BKCa) and voltage activated Na⁺+ channel (INa) present in human umbilical cord vein-derived mesenchymal cells (hUC-MSCs). However, little is known of single channel characteristic of BKCa in hUC-MSCs. The pharmacological action of riluzole, a drug used for the treatment of amyotrophic lateral sclerosis, has not yet been investigated in stem cells. In this study, we therefore studied effects of riluzole on functional ion channels, BKca and INa, in hUC-MSCs using single channel and/or whole cell configuration. Single channel recording revealed 244.45±29.25 pS of Ca²⁺ and voltage-gated K⁺ channel present in hUC-MSCs and the value is similar to that of typical BKCa channel reported in other cells. Riluzole (100 µM) increased BKCa in cell-attached/whole cell patch configuration. The activation effect of riluzole on this ion channel also found in insideout configuration, suggesting that riluzole may directly activate BKCa in hUC-MSCs. On the contrary, riluzole inhibited INa in a concentration-dependent manner with an IC₅₀ value of 16 µM. Taken together, BKCa and INa in hUC-MSCs can be an important target for the action of riluzole and the channels are partly may contribute to maintaining homeostasis by regulation of ion flux in hUC-MSCs.
Jungsoo Lee,Yangmi Park,Hyunkee Kim,Nakhoon Kim,Wonjae Sung,Sanggon Lee,박진석 대한임상신경생리학회 2021 Annals of Clinical Neurophysiology Vol.23 No.2
Background: Nitrous oxide (N2O) is used in surgery and dentistry for its anesthetic and analgesic effects. However, neurological and psychiatric manifestations of N2O abuse have been increasingly reported among Korean adults. The aim of this study was to demonstrate laboratory findings of N2O abuse in Korean patients. Methods: Patients diagnosed with N2O-induced neuropathy or myelopathy from August 2018 to December 2019 were enrolled. Their clinical presentations and laboratory and imaging findings were analyzed. Results: Sensory changes and limb weakness were present in nine of the enrolled patients. The laboratory findings revealed that seven patients had high homocysteine levels and five had high methylmalonic acid levels in their blood. Nerve conductions studies indicated that axonal neuropathy was present in four cases and longer F-wave and Hoffman’s-reflex latencies were present in two cases. Signal changes in cervical spine imaging occurred in five patients, while two had normal results. Conclusions: Chronic N2O abuse can cause neurological damage or psychiatric problems. Because N2O is illegal for recreational use in Korea, patients tend to hide their history of use. Even though the spinal imaging results were normal, clinicians should consider the possibility of N2O use, and further electrophysiological tests should be applied for precise evaluations.
Kyoung Sun Park,Hyoweon Bang,Eun-Young Shin,Chan Hyung Kim,Yangmi Kim 대한생리학회-대한약리학회 2008 The Korean Journal of Physiology & Pharmacology Vol.12 No.4
TREK (TWIK-RElated K<sup>+</sup> channels) and TRAAK (TWIK-Related Arachidonic acid Activated K<sup>+</sup> channels) were expressed in COS-7 cells, and the channel activities were recorded from inside-out membrane patches using holding potential of −40 mV in symmetrical 150 mM K<sup>+</sup> solution. Intra</SUP>cellular application of an oxidizing agent, 5,5 -dithio-bis (2-nitrobenzoic acid) (DTNB), markedly decreased the activity of the TREK2, and the activity was partially reversed by the reducing agent, dithiothreitol (DTT). In order to examine the possibility that the target sites for the oxidizing agents might be located in the C-terminus of TREK2, two chimeras were constructed: TREK2 (1-383)/TASK3C and TREK2 (1-353)/TASK3C. The channel activity in the TREK2 (1-383)/TASK3C chimera was still inhibited by DTNB, but not in the TREK2 (1-353)/TASK3C chimera. These results indicate that TREK2 is inhibited by oxidation, and that the target site for oxidation is located between the amino acid residues 353 and 383 in the C-terminus of the TREK2 protein.