Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

홈쇼핑 기업과 소비재 중소기업의 해외 동반진출에 관한 사례연구

정소원(So Won Jeong),양희순(Heesoon Yang),정재은(Jae Eun Chung) 한국경영학회 2017 한국경영학회 통합학술발표논문집 Vol.2017 No.08

우리나라의 지속적인 경제발전과 경쟁력을 확보하기 위해서는 대기업과 중소기업의 간의 균형 잡힌 발전이 필요하다. 이에 대중소기업간 상생 및 해외 동반진출에 대한 관심이 높으며, 정부에서도 여러 가지 지원정책을 늘리고 있다. 이에 본 연구에서는 국내 홈쇼핑 기업과 소비재 중소기업의 해외 동반진출에 관한 인터뷰를 통해 홈쇼핑의 특수성을 고려한 효율적인 유통대기업과 중소기업의 해외 동반진출 확대 방안을 모색하고자 한다. 이를 위해 해외 동반진출을 하고 있는 3개의 홈쇼핑 기업을 대상을 인터뷰를 실시하였으며, 결과는 다음과 같다. 홈쇼핑 기업은 소비재 중소기업의 해외 판로 개척에 도움이 되며, 자원과 역량이 부족한 중소기업에게 경영 및 마케팅 역량을 활용할 수 있게 해준다. 홈쇼핑 기업이 소비재 중소기업을 지원하기 위해 영상을 제작하거나 언어를 번역해주기도 하며, 무역 통관과 관련된 서류를 지원해주기도 한다. 또한 시장개척단을 구성하여 해외시장 정보 제공 등의 지원을 한다. 그렇지만 아직까지는 홈쇼핑 기업에게 해외 동반진출이 주는 실질적인 혜택이 미미하기 때문에 장기적으로 서로 win-win할 수 있는 전략이 필요하다. 이를 위해 해외 동반진출을 독려할 수 있도록 실질적인 혜택을 제공해야 하며, 궁극적으로 CSV 개념을 실천할 수 있도록 해야 한다. There must be a balanced development of both conglomerates and small and mid-sized companies in order to secure constant economic growth and competitive edge of South Korea. Accordingly, high expectations are being placed on win-win growth and joint overseas expansion of conglomerates and small and mid-sized companies. This study seeks efficient ways to promote joint overseas expansion of major retailers and small and mid-sized companies considering the distinctiveness of home shopping by conducting interviews about joint overseas expansion of home shopping companies and small and mid-sized consumer goods companies in South Korea. Home shopping companies contribute to opening up overseas markets for small and mid-sized consumer goods companies, and allows them to make use of business and marketing competencies that they lack. Home shopping companies also produce visual materials or provide language translations, and help draw up documents for customs clearance in trading. They also form market development teams and provide information about the overseas markets. However, since the actual benefits from joint overseas expansion are minor for home shopping companies, there is a need for a strategy for win-win growth of both parties in the long run. To this end, it is necessary to provide substantial benefits to encourage joint overseas expansion and ultimately implement the concept of creating shared value (CSV).

소아 알레르기비염의 경제적 부담 평가를 위한 다기관 조사

공도연 ( Do Youn Kong ),김경원 ( Kyung Won Kim ),김우경 ( Woo Kyung Kim ),민택기 ( Taek Ki Min ),박용민 ( Yong Mean Park ),안재억 ( Jae Ouk Ahn ),양현종 ( Hyeon Jong Yang ),염혜영 ( Hye Yung Yum ),윤혜선 ( Hae Sun Yoon ),전유훈 ( 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2012 소아알레르기 및 호흡기학회지 Vol.22 No.2

목적: 알레르기비염의 유병률은 전 세계적으로 급격히 증가하고 있으며, 그에 따른 경제적 부담도 증가하고 있다. 그러나, 소아 알레르기 질환이 미치는 경제적 부담에 관한 국내 연구는 제한적이어서 소아 알레르기비염이 환자와 그 가족에게 미치는 경제적 부담을 산출하고자 본 연구를 진행하였다. 방법: 2008년 7월 1일부터 9월 31일까지 서울시 6개 2-3차 의료기관을 방문한 18세 이하 소아 알레르기비염 환자 및 보호자를 대상으로 설문 조사를 진행하였다. 설문을 통해 직접 의료비 (병·의원 진료비, 및 약제비 한방 진료비 및 약제비, 보완/대체요법비), 직접비의료비 (교통비, 환경개선비), 그리고 간접비용 (월 평균 노동 손실)을 조사하였고 그 결과를 질환의 중증도 및 유병 기간에 따라 분석하여 그 차이를 비교하였다. 결과: 모집된 262명의 대상 중 174명(66.4%)이 남자였고, 평균 연령은 6.54세였다. 대상군의 연간 평균 직접 의료비는 177만 원이었고 직접비 의료비는 57만 원이었다. 비록 통계학적 유의성은 없었지만, 알레르기비염의 중증도가 증가할수록 직접 의료비가 증가하는 경향을 보였고, (P=0.053) 유병 기간 또한 직접 의료비의 증가와 유의한 양의 상관 관계를 보였다. (R=0.195, P=0.002) 대상 환자 보호자의 약 17%가 아이의 알레르기비염으로 인해 직장에 결근 또는 조퇴를 경험한 것으로 조사되어 사회적 간접비용을 미루어 생각할 수 있었다. 결론: 알레르기비염이 미치는 경제적 부담은 질환의 중증도가 심할수록, 그리고 유병 기간이 길수록 증가하며, 이는 특히 한방 진료 및 치료비와 보완/대체요법비의 증가에 기인한 것으로 조사되었다. 따라서 알레르기비염으로 인한 경제적 부담을 줄이기 위해서는 환자와 보호자를 대상으로 근거 중심의 치료를 할 수 있도록 지속적이고 체계적인 교육이 필요할 것으로 사료된다. Purpose: The prevalence of allergic rhinitis is rapidly increasing and results in relatively high socio-economic burden on their family and community. However, studies on the economic burden of pediatric allergic rhinitis in Korea are limited. Therefore, we conducted this study to investigate the impact of pediatric allergic rhinitis on economic burden. Methods: Two hundred sixty two children with allergic rhinitis were enrolled in 6 secondary or tertiary medical centers in Seoul from July to September, 2008. We collected data of the economic burden of allergic rhinitis (direct medical costs, direct nonmedical costs, and indirect costs) by face to face questionnaire survey. We compared the economic burden according to the severity and the duration of allergic rhinitis. Results: The mean age of subjects was 6.54 years, and male were 174 (66.4%). Direct medical costs (10,000 Korean Won/yr) were 177.75, and direct nonmedical costs were 57.92. Although, there was no statistical significance, direct medical costs showed increasing trends in severe allergic rhinitis. (P=0.053) In addition, direct medical costs were positively correlated with duration of allergic rhinitis.( R=0.195, P=0.002). About 17% of the parents who care the allergic rhinitis children experienced the work absence due to their child s illness. Conclusion: The economic burdens of allergic rhinitis were positively correlated with the severity and duration of illness. Particularly costs for alternative medicine including oriental medicine` were related with severity and duration allergic rhinitis. Therefore, special efforts for education with evidence based treatment strategy are necessary to decrease the economic burden of allergic rhinitis.

Tissue distribution of LOM strain viral antigens using immunohistochemistry in weaned pigs in Jeju

Jae-Sung Heo(Jae-Sung Heo),Jae-Beum Kim(Jae-Beum Kim),Yun-Ho Kim(Yun-Ho Kim),Hyoung-Nam Jo(Hyoung-Nam Jo),Won-Gyu Cho(Won-Gyu Cho),Myeong-Won Suh(Myeong-Won Suh),Hyoung-Seok Yang(Hyoung-Seok Yang),Wan 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-

Immunohistochemical detection of LOM strain viral antigens in suckling piglets in Jeju

Jae-Beum Kim(Jae-Beum Kim),Won-Gyu Cho(Won-Gyu Cho),Yun-Ho Kim(Yun-Ho Kim),Hyoung-Nam Jo(Hyoung-Nam Jo),Jae-Sung Heo(Jae-Sung Heo),Myeong-Won Suh(Myeong-Won Suh),Hyoung-Seok Yang(Hyoung-Seok Yang),Wan 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-

The Nevus Lipomatosus Superficialis of Face: A Case Report and Literature Review

Jae-Won Yang,Mi-Ok Park Korean Society of Plastic and Reconstructive Surge 2024 Archives of Plastic Surgery Vol.51 No.2

Nevus lipomatosus superficialis (NLS) is a hamartoma of adipose tissue, rarely reported in the past 100 years. We treated one case, and we conducted a systematic review of the literature. A 41-year-old man presented with a cutaneous multinodular lesion in the posterior region near the right auricle. The lesion was excised and examined histopathologically. To review the literature, we searched PubMed with the keyword "NLS." The search was limited to articles written in English and whose full text was available. We analyzed the following data: year of report, nation of corresponding author, sex of patient, age at onset, duration of disease, location of lesion, type of lesion, associated symptoms, pathological findings, and treatment. Of 158 relevant articles in PubMed, 112 fulfilled our inclusion criteria; these referred to a total of 149 cases (cases with insufficient clinical information were excluded). In rare cases, the diagnosis of NLS was confirmed when the lesion coexisted with sebaceous trichofolliculoma and Demodex infestation. Clinical awareness for NLS has increased recently. NLS is an indolent and asymptomatic benign neoplasm that may exhibit malignant behavior in terms of huge lesion size and specific anatomical location. Early detection and curative treatment should be promoted.

Vertically aligned carbon nanotubes grown on various substrates by plasma enhanced chemical vapor deposition

Jae-hee Han,Byung-Sik Moon,Won-Suk Yang,Ji-Beom Yoo,Chong-Yun Park,In-Taek Han,Nae-Sung Lee,Chong-Min Kim,Tae-Il Kim 한국진공학회(ASCT) 1999 Journal of Korean Vacuum Science & Technology Vol.3 No.2

Vertically well aligned multiwall carbon nanotubes were grown on nickel coated different substrates by plasma enhanced hot filament chemical vapor deposition at low temperatures below 650℃. Acetylene and ammonia gas were used as the carbon source and a catalyst. The surface roughness of nickel layer increased as NH₃ etching time increased. The diameters of the nanotubes decreased and the density of nanotubes increased as NH₃ etching time increased. Diameter of nanotube was 30 to 70 ㎚. Nickel cap was observed on the top of the grown nanotube and very thin carbon amorphous layer was found on the nickel cap.

Relationship between serum brain natriuretic peptide and heart function in patients with chronic kidney disease

( Jae Won Yang ),( Min Soo Kim ),( Jae Seok Kim ),( Jong Myoung Yoo ),( Seung Tae Han ),( Bi Ro Kim ),( Yun Deok Kim ),( Jeong Wook Choi ),( Seung Ok Choi ),( Byoung Geun Han ) 대한내과학회 2008 The Korean Journal of Internal Medicine Vol.23 No.4

Background/Aims: Brain natriuretic peptide (BNP) levels are known to be elevated in patients with chronic kidney disease (CKD) and normal heart function. Therefore, the present study was performed to examine the effectiveness of BNP level in diagnosing heart failure in patients with CKD and to determine its effects on survival rate and prognosis. Method: A total of 182 patients with CKD who visited the hospital due to dyspnea of NYHA class II were included in the study. BNP levels were measured and echocardiography was performed to divide the subjects into groups with and without heart failure. Their BNP levels, clinical courses, and survival rates were analyzed retrospectively. Results: When BNP level was ≥858.5 pg/mL in CKD patients, heart failure could be diagnosed with sensitivity and specificity of 77% and 72%, respectively. Survival rate of the group with BNP levels of ≥858.8 pg/mL was significantly lower than that of the group with BNP level below this threshold (p=0.012) and multivariate analysis showed that BNP level, age, and sex affected survival rate in the group with BNP level ≥858.8 pg/mL. Conclusions: BNP levels of patients with CKD showed a positive correlation with creatinine levels, and the critical point of BNP level for diagnosis of heart failure was 858.5 pg/mL. As the survival rate in patients with BNP level above the critical point was significantly low, this level was a useful indicator for predicting their prognosis. Care should be taken in interpreting BNP level because patients with stage 5 CKD may show a high concentration of BNP without heart failure.

Rosacea-Like eruption associated with nilotinib (초)

( Jae Kyung Kim ),( Ji Hye Yang ),( Deok Woo Lee ),( Chong Hyun Won ),( Sung Eun Chang ),( Mi Woo Lee ),( K H Lee ),( Jee Ho Choi ),( Kee Chan Moon ) 대한피부과학회 2011 초록집 Vol.49 No.10

Foreign body granuloma caused by hyaluronic acid/dextranomer microsphere filler injection (초)

( Jae Kyung Kim ),( Ji Hye Yang ),( Sang Min Lee ),( Chong Hyun Won ),( Sung Eun Chang ),( Mi Woo Lee ),( Jee Ho Choi ),( Kee Chan Moon ) 대한피부과학회 2011 초록집 Vol.49 No.10