Research on Preparation Methods of Ultrafine Softwood Powder

Changsheng Fan,Dongxia Yang,Hongling Wang,Yan Sun,Hua lou,Hongru Yang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.4

Pine wood sawdust is used as raw materials for experimental processing into ultrafine wood powder. Sizes of ultrafine wood powder particles serve as a standard for measuring ultrafine processing. The core part of the experimental processing equipment is the millstone which can exert great shear force and grinding force on strong fiber materials so as to ensure the successful preparation of ultrafine particles. The “equilibrium orbit” model is used to simulate processed superfine particles in calculating separation performance. Moreover, the CFD is chosen for simulating separation performance of wood powder particles with different sizes in the two-phase flow field, so as to ensure the successful separation and grading of wood powder particles with different sizes in the practical processing. Research is made on physical properties of collected wood powder as samples with different sizes and influence on composite material properties when ultrafine wood powder is taken as padding. Research on ultrafine wood powder provides meaningful experimental data and theoretical support for the future research on micro-nano fibrils.

Investigation of morphological changes of HPS membrane caused by cecropin B through scanning electron microscopy and atomic force microscopy

Han Hu,Changsheng Jiang,Binzhou Zhan,Nan Guo,Zhonghua Li,Xiaozhen Guo,Yang Wang,Binlei Liu,Qigai He 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.5

Background: Antimicrobial peptides (AMPs) have been identified as promising compounds for consideration as novel antimicrobial agents. Objectives: This study analyzed the efficacy of cecropin B against Haemophilus parasuis isolates through scanning electron microscopy (SEM) and atomic force microscopy (AFM) experiments. Results: Cecropin B exhibited broad inhibition activity against 15 standard Haemophilus parasuis (HPS) strains and 5 of the clinical isolates had minimum inhibition concentrations (MICs) ranging from 2 to 16 μg/mL. Microelectrophoresis and hexadecane adsorption assays indicated that the more hydrophobic and the higher the isoelectric point (IEP) of the strain, the more sensitive it was to cecropin B. Through SEM, multiple blisters of various shapes and dents on the cell surface were observed. Protrusions and leakage were detected by AFM. Conclusions: Based on the results, cecropin B could inhibit HPS via a pore-forming mechanism by interacting with the cytoplasmic membrane of bacteria. Moreover, as cecropin B concentration increased, the bacteria membrane was more seriously damaged. Thus, cecropin B could be developed as an effective anti-HPS agent for use in clinical applications.

Study of Hair Melanins in Various Hair Color Alpaca (Lama Pacos)

Fan, Ruiwen,Yang, Gang,Dong, Changsheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4

The aim of this study was to measure the hair melanins of various colors and to find the relationship between the quantity of melanins and hair color phenotypes in alpacas. According to the Munsell color system, 3 healthy alpacas were selected for each of the 22 different hair color phenotypes (66 alpacas altogether). Alpaca hair was taken from the lateral thoracic region and then dissolved with different solutions to obtain melanins. The values of alkali-soluble melanins (ASM), eumelanin (EM) and pheomelanin (PM) were measured by spectrophotometric assay, and labeled as Sp.ASM, Sp.EM and Sp.PM, respectively. Data were analyzed using SPSS11.5 software. Results showed that average Sp.ASM and Sp.PM were increased as the color deepened from white to black, ranging from 0.500 to 4.543 for Sp.ASM and from 0.268 to 1.457 for Sp.EM. However, average Sp.PM had no such apparent relationship with color. Based on the value of Sp.ASM and EM, 7 hues were produced and gray was a single hue. Most of the data were in a normal distribution (p>0.10). ANOVA analysis showed that mean values of Sp.ASM, Sp.EM and Sp.PM were significantly different (p<0.05). The results also showed that Sp.ASM was positively correlated with Sp.EM but the correlation between Sp.ASM and Sp.PM was not significantly different from 0. It is concluded that EM is the major constituent of alpaca hair melanin; there is a significant correlation among ASM, EM and alpaca hair colors, and EM is the most reliable parameter for distinguishing these groups.

Effect of In-Situ Ti2N on Microstructure and Properties of SAF2906 Duplex Stainless Steel Fabricated by Laser Melting Deposition

Jing Liang,Sheng Yang,Ye Liu,Xiuyuan Yin,Suiyuan Chen,Changsheng Liu 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.7

SAF2906 Duplex Stainless Steel (DSS) was fabricated by powder prepasted Laser Melting Deposition(LMD). The formationof ferrite was promoted by increasing the molybdenum content. The volume ratio of ferrite and austenite of LMDed SAF2906DSS with 3 wt% molybdenum content reached 4:6. The effects of 2 wt% and 4 wt% nano TiN addition on the microstructureand comprehensive properties of LMDed SAF2906 DSS were investigated in detail. The results showed that in-situ generatednano (10 nm ~ 100 nm) and submicron (100 nm ~ 1 μm) Ti2Nparticles were uniformly distributed in the LMDed SAF2906DSS matrix, and some nano Ti2Nparticles preferred to be distributed among grain boundaries. The primary and secondarydendrite spacing of austenite of LMDed SAF2906 with 4wt% TiN addition decreased by 48% and 62%, respectively, whilethe austenite content increased by 15%. The wear resistance, strength, elongation and corrosion resistance of the LMDedSAF2906 were gradually improved with the increase of nano TiN addition from 0 to 4 wt%. The average microhardnessfor the sample with 4 wt% nano TiN addition (381 HV0.2)was 1.2 times that of the sample without TiN (326 HV0.2),whilethe wear rate (9.12 × 10–13 m3/Nm) was only 71% that of the sample without TiN (1.28 × 10–12 m3/Nm). The yield strength,tensile strength and elongation of the LMDed SAF2906 with 4wt% TiN addition increased to 801 MPa, 1028 MPa and31.8%, respectively. The self-corrosion potential increased to 0.0216 V, and the self-corrosion current density also reached4.57 × 10–7 A·cm−2 for LMDed SAF2906 with 4wt% TiN addition.

PAX8-AS1 knockdown facilitates cell growth and inactivates autophagy in osteoblasts via the miR-1252-5p/GNB1 axis in osteoporosis

Huang Caiqiang,Li Runguang,Yang Changsheng,Ding Rui,Li Qingchu,Xie Denghui,Zhang Rongkai,Qiu Yiyan 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Osteoporosis (OP) is the most common systematic bone disorder among elderly individuals worldwide. Long noncoding RNAs (lncRNAs) are involved in biological processes in various human diseases. It has been previously revealed that PAX8 antisense RNA 1 (PAX8-AS1) is upregulated in OP. However, its molecular mechanism in OP remains unclear. Therefore, we specifically designed this study to determine the specific role of PAX8-AS1 in OP. We first established a rat model of OP and then detected PAX8-AS1 expression in the rats with RT-qPCR. Next, to explore the biological function of PAX8-AS1 in osteoblasts, in vitro experiments, such as Cell Counting Kit-8 (CCK-8) assays, flow cytometry, western blotting and immunofluorescence (IF) staining, were conducted. Subsequently, we performed bioinformatic analysis and luciferase reporter assays to predict and identify the relationships between microRNA 1252-5p (miR-1252-5p) and both PAX8-AS1 and G protein subunit beta 1 (GNB1). Additionally, rescue assays in osteoblasts clarified the regulatory network of the PAX8-AS1/miR-1252-5p/GNB1 axis. Finally, in vivo loss-of-function studies verified the role of PAX8-AS1 in OP progression. The results illustrated that PAX8-AS1 was upregulated in the proximal tibia of OP rats. PAX8-AS1 silencing promoted the viability and inhibited the apoptosis and autophagy of osteoblasts. PAX8-AS1 interacted with miR-1252-5p. GNB1 was negatively regulated by miR-1252-5p. In addition, the impacts of PAX8-AS1 knockdown on osteoblasts were counteracted by GNB1 overexpression. PAX8-AS1 depletion suppressed OP progression by inhibiting apoptosis and autophagy in osteoblasts. In summary, PAX8-AS1 suppressed the viability and activated the autophagy of osteoblasts via the miR-1252-5p/GNB1 axis in OP.

Effects of electroslag remelting process and Y on the inclusions and mechanical properties of the CLAM steel

Qiu, Guoxing,Zhan, Dongping,Li, Changsheng,Yang, Yongkun,Jiang, Zhouhua,Zhang, Huishu Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.4

Y-containing CLAM steels were melted via vacuum induction melting and electroslag remelting. In this study, the evolution, microstructure, and mechanical properties of the alloy inclusions (ESR-1 (0 wt.% Y), ESR-2 (0.016 wt.% Y) and ESR-3 (0.042 wt.% Y)) were investigated. Further, the number of inclusions in ESRed steel was observed to obviously decrease, and the distributions were more uniform. The fine Y-Al-O inclusions (1-2 ㎛) were the main inclusions in ESR-2. The addition of Y affected the prior austenite grain size (PAGZ), increasing the tensile strength at test temperature. Low ductile-brittle transition temperature (DBTT) was obtained because of the fine PAGZ and dispersive inclusions. For the ESRed CLAM steel with 0.016 wt.% Y, the yield strengths were 621 MPa at 20 ℃ and 354 MPa at 600 ℃ in air. Further, the uniform elongation and elongation of the ESR-2 alloy were 5.5% and 20.1% at 20 ℃, respectively. Meanwhile, the DBTT tested using full-size Charpy impact specimen (55 cm × 10 cm × 10 cm) was reduced to -83 ℃.

Validation of Methods for Isolation and Culture of Alpaca Melanocytes: A Novel Tool for In vitro Studies of Mechanisms Controlling Coat Color

Bai, Rui,Sen, Aritro,Yu, Zhihui,Yang, Gang,Wang, Haidong,Fan, Ruiwen,Lv, Lihua,Lee, Kyung-Bon,Smith, George W,Dong, Changsheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4

The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat color and to determine the effect of alpha melanocyte stimulating hormone treatment on mRNA expression for the melanocortin 1 receptor, a key gene involved in coat color regulation in other species. Skin punch biopsies were harvested from the dorsal region of 1-3 yr old alpacas and three different enzyme digestion methods were evaluated for effects on yield of viable cells and attachment in vitro. Greatest cell yields and attachment were obtained following dispersion with dispase II relative to trypsin and trypsin-EDTA treatment. Culture of cells in medium supplemented with basic fibroblast growth factor, bovine pituitary extract, hydrocortisone, insulin, 12-O-tetradecanolphorbol-13-acetate and cholera toxin yielded highly pure populations of melanocytes by passage 3 as confirmed by detection of tyrosinase activity and immunocytochemical localization of melanocyte markers including tyrosinase, S-100 and micropthalmia-associated transcription factor. Abundance of mRNA for tyrosinase, a key enzyme in melanocyte pigment production, was maintained through 10 passages showing preservation of melanocyte phenotypic characteristics with extended culture. To determine hormonal responsiveness of cultured melanocytes and investigate regulation of melanocortin 1 receptor expression, cultured melanocytes were treated with increasing concentrations of ${\alpha}$-melanocyte stimulating hormone. Treatment with ${\alpha}$-melanocyte stimulating hormone increased melanocortin receptor 1 mRNA in a dose dependent fashion. The results demonstrated culture of pure populations of alpaca melanocytes to 10 passages and illustrate the potential utility of such cells for studies of intrinsic and extrinsic regulation of genes controlling pigmentation and coat color in fiber-producing species.

Risk of New Vertebral Fracture and Combination Therapy with Zoledronic Acid and Teriparatide in Diabetic Patients after Percutaneous Kyphoplasty

Zhang Jian,Yan Bin,Chen Zhe,Zheng Zhaomin,Yang Changsheng 대한척추외과학회 2021 Asian Spine Journal Vol.15 No.5

Study Design: This was a retrospective clinical study. Purpose: This study aimed to evaluate the effect of combination therapy with zoledronic acid and teriparatide on the risk of new vertebral fracture (NVF) in type 2 diabetes mellitus (T2DM) patients after percutaneous kyphoplasty (PKP). Overview of Literature: Although T2DM had been associated with bone fragility and increased fracture risk, it remains unknown whether patients with T2DM could expect similar benefit from the combination therapy with zoledronic acid and teriparatide following PKP. Methods: Total 106 diabetic patients who had undergone PKP and had received anti-osteoporosis treatment for osteoporotic vertebral compression fracture were enrolled and allocated into the following two groups: group I (n=52, zoledronic acid) and group II (n=54, zoledronic acid plus teriparatide). The operating time, bone cement volume, and complications related to anti-osteoporosis treatment or PKP, if any, were recorded. The Visual Analog Scale (VAS) score and Oswestry Disability Index (ODI) were assessed at admission, at discharge, and at the final follow-up. Dual-energy X-ray absorptiometry scan of the hip for the measurement of the bone mineral density (BMD) was performed preoperatively and at the final follow-up for all the patients. Results: There was no significant difference in the age, body mass index, bone cement volume, or follow-up time of the groups. The mean follow-up duration was 22.5±1.6 months. All the patients had improved VAS and ODI, and group II had significantly better clinical outcomes than group I. All the patients had increased BMD at the latest follow-up, while group II exhibited significantly more improvement. The prevalence of NVF was lower in group II (11.5% vs. 7.4%, p=0.523). Male patients had a higher prevalence of NVF although the difference was not statistically significant. Conclusions: Combination therapy with zoledronic acid and teriparatide could improve the clinical outcomes, and BMD and had the potential to reduce NVF in diabetic patients following PKP.