Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers

Yan‐xin Zhang,Wei Hua,Lin‐han Wang,Zhuo Che,Xiu‐rong Zhang 한국유전학회 2010 Genes & Genomics Vol.32 No.3

The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard’s genetic similarity coefficients (GS=0.7130), Nei's gene diversity (h=0.2418) and Shannon's Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro‐ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro‐ecological zones, the maximum Nei’s gene diversity (h = 0.2613)and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei's genetic identity and genetic distance among landraces from seven agro‐ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro‐ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable utilization of these sesame landraces in breeding.

가정교육투자가 중국 초등학생 학습성적에 미치는 영향에 관한 연구: 학습자질 매개효과 연구

Yan Wang,이승진 국제인문사회연구학회 2024 Studies on Humanities and Social Sciences (SHSC) Vol.6 No.1

A Study on the Effect on Education Investment in Family on the Academic Performance of Elementary School Students in China: The Mediating Effect on Approaches to Learning Yan Wang** & Seungjin Lee*** Abstract: This study analyzed the effect of children’s learning capacity on the relationship between Chinese primary school students’ parents’ financial and time investment in home education and their children’s Chinese and Mathematics performances. For this first, an analysis of age and gender differences in learning capacity was conducted targeting 181 elementary school students from two public primary schools located in Xi'an, China. Second, the direct effect of parents’ financial and time home education investment on primary school children’s test performance (Chinese and Mathematics) was analyzed. And the indirect effect on children’s test performance mediated by approaches to learning were analyzed. For difference analysis on gender and age, an independen-samples t test was conducted, and path analysis was conducted to analyze the structure between financial and time home education investment, approaches to learning, and test performance. As a result of the study, first, it was found that girls had higher lapproaches to learning than boys, and higher grades (4th grade) had higher approaches to learning than lower grades (1st grade). Second, approaches to learning had a direct effect on test performance (Chinese, mathematics), while financial family investment did not show a direct effect on test performance. On the other hand, in the case of time home education investment, it not only had a direct effect on academic performance, but also had an indirect effect on test performance through approaches to learning, and the indirect effect was found to be higher than the direct effect. This study suggested to parents of primary school children in China the importance of forming approaches to learning on their children's approaches to learning, and provides basic data for planning financial and time invesments in home education decisions in forming approaches to learning. Key Words: Approaches to Learning, Investment in Family Education, Financial Investment in Family Education, Time Investment in Family Education, Primary School Studetns in China □ 접수일: 2024년 1월 22일, 수정일: 2024년 2월 14일, 게재확정일: 2024년 2월 20일* 이 논문은 2024학년도 세한대학교 교내연구비 지원으로 작성된 것임. ** 주저자, 서안외사대학 교육학과 부교수(First Author, Professor, Xi’an International Univ.)*** 교신저자, 세한대학교 조교수(Correponding Author, Professor, Sehan Univ., Email: seunjgincool@gmail.com) 가정교육투자가 중국 초등학생 학습성적에 미치는 영향에 관한 연구: 학습자질 매개효과 연구 왕 연ㆍ이 승 진 연구 목적: 본 연구는 중국 초등학생 부모의 재정 및 시간적 가정교육투자와 자녀의 국어, 수학 성적 관계에 있어 자녀의 학습자질이 미치는 영향력을 분석하였다. 연구 방법: 중국 서안시에 위치한 두 공립초등학교 초등학생 181명을 대상으로 첫째, 학습자질에 있어 연령과 성별 차이분석을 실시하였고 둘째, 부모의 재정 및 시간적 가정교육투자가 초등학생 자녀의 시험성적(국어와 수학)에 미치는 직접효과와 학습자질을 매개로 미치는 간접효과를 분석하였다. 차이분석의 경우 독립표본 t 테스트를 실시하였고 재정 및 시간적 가정교육투자, 학습자질, 학습성적간 구조분석을 위해 경로분석을 실시하였다. 연구 내용: 연구결과 첫째, 여아가 남아보다, 고학년(4학년)이 저학년(1학년)보다 학습자질이 높은 것으로 나타났고 둘째, 학습자질은 학업업성적(국어, 수학)에 직접효과가 있는 반면 재정적 가정투자는 학업성적에 직접효과는 없는 것으로 나타났다. 시간적 가정교육투자의 경우 학업성적에 직접효과 뿐만 아니라 학습자질을 매개로 학업성적에 간접효과가 있었고 간접효과가 직접효과보다 높았다. 결론 및 제언: 본 연구는 중국 초등학생 자녀를 둔 학부모들에게 자녀들의 학습능력에 미치는 학습자질 형성의 중요성을 시사하였고 학습자질에 형성에 있어 재정적, 시간적 가정교육투자 결정 계획에 기초자료를 제공하였다. 핵심어: 가정교육투자, 재정적 가정교육투자, 시간적 가정교육투자, 학습자질, 중국 초등학생

Nodule Rich Protein 2 modulates nodule number in Medicago truncatula

Junhui Yan,Xinwei Yang,Yawen Wang,Liangliang Yu,Li Luo 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.1

Symbiotic nitrogen fxation is beneft to sustainable agriculture and global nitrogen cycle. Many small peptides were identifed as regulators involving in the interaction between rhizobia and legume. Here we reported Nodule Rich Protein 2 (MtNRP2) encoding a small peptide in Medicago truncatula, belonged to a group of nodule rich protein restricted in legume species. MtNRP2 expressed highly in root nodule and its promoter was active during the initiation and development of root nodule and lateral root. To investigate the function of MtNRP2 in nodulation, we generated MtNRP2-overexpression and MtNRP2- knockdown transgenic Medicago. MtNRP2-overexpression transgenic lines performed normal nodulation phenotype compared with vector control. However, in the MtNRP2-RNAi transgenic plants, the decrease of MtNRP2 expression lead to the increase of infection threads number (7 day post inoculation) and nodules number (3 week post inoculation); meanwhile, the expression of MtRGF3 and MtPUB1 was inhibited. These results suggested that MtNRP2 negatively regulated nodulation in Medicago truncatula.

Two New Species of Placolecis (Lichenized Ascomycota) from China

( An Cheng Yin ),( Xin Yu Wang ),( Dong Liu ),( Yan Yun Zhang ),( Mei Xia Yang ),( Li Juan Li ),( Li Song Wang ) 한국균학회 2019 Mycobiology Vol.47 No.4

Two new species of the lichen genus Placolecis are discovered in China, namely P. kunmingensis An. C. Yin & Li S. Wang and P. sublaevis An. C. Yin & Li S. Wang. The new combination P. loekoesiana (S.Y. Kondr., Farkas, J.J. Woo & Hur) An. C. Yin is proposed. Placolecis kunmingensis is characterized by having simple, spherical or ellipsoid, hyaline spores, and pear-shaped pycnidia; while P. sublaevis can be distinguished by its thallus forming larger aggregations with slightly flattened lobes at the thallus margin, and urn-shaped pycnidia. Descriptions, a phylogenetic tree and a key are provided for all the known Placolecis species in China.

Spontaneous wrinkling pattern of a constrained thin film membrane

Yan, Yuan,Wang, Binglei,Yin, Jie,Wang, Tiejun,Chen, Xi Springer-Verlag 2012 APPLIED PHYSICS A MATERIALS SCIENCE AND PROCESSING Vol.107 No.4

Stathmin is a Marker of Progression and Poor Prognosis in Esophageal Carcinoma

Wang, Feng,Xuan, Xiao-Yan,Yang, Xuan,Cao, Lei,Pang, Li-Na,Zhou, Ran,Fan, Qin-Xia,Wang, Liu-Xing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Stathmin, also called oncoprotein 18, is a founding member of the family of microtubule-destabilizing proteins that play a critical role in the regulation of mitosis. At the same time stathmin has been recognized as one of responsible factors in cancer cells. The aim of this study was to assess stathmin status, its correlations with clinicopathological parameters and its role as a progosnostic marker in EC patients. The protein and mRNA levels of stathmin were examined byimmunohistochemistry (IHC) and in situ hybridization in 100EC tissues and adjacent noncancerous tissues. mRNA and protein expression of stathmin in three EC cell lines(EC9706, ECa109, EC1 commonly used in research) were also analyzed using immunocytochemistry, western blot and in situ hybridization. The prognostic value of Stathmin expression within the tumor tissues were assessed by Cox regression and Kaplan-Meier analysis. We showed that stathmin expression was significantly higher in EC tissues than in adjacent noncancerous tissues. High stathmin immunostaining score in the EC was positively correlated with tumor differentiation, Tumor invasion, Lymph node metastases, and TNM stage. In addition, we demonstrated that three EC cell lines examined, were constitutively expressing a high level of stathmin. Of those, EC-1 showed the strongest mRNA and protein expression for the stathmin analyzed. Kaplan-Meier analysis showed that significantly longer 5-year survival rate was seen in EC patients with high Stathmin expression, compared to those with low expression of Stathmin expression. Furthermore, multivariate Cox proportional hazard analyses revealed that Stathmin was an independent factors affecting the overall survival probability. In conclusion, our data provide a basis for the concept that stathmin might be associated with EC development and progression. High levels of Stathmin expression in the tumor tissues may be a good prognostic marker for patients with EC.

Preliminary Study on Hairy Root Occurrence of Tree Peony Induced by Agrobactrium rhizogenes

Yan Wang,Zhi-de Yang,Kun Miao,Qiao-xiang Tang,Xue-mei Liu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Glechomanamides A-C, Germacrane Sesquiterpenoids with an Unusual Δ⁸-7,12-Lactam Moiety from <i>Salvia scapiformis</i> and Their Antiangiogenic Activity

Wang, Cai Yi,Kim, Donghwa,Zhu, Yan Kui,Oh, Dong-Chan,Huang, Ri Zhen,Wang, Heng-Shan,Liang, Dong,Lee, Sang Kook American Society of Pharmacognosy 2019 Journal of natural products Vol.82 No.11

<P>Three new germacrane sesquiterpenoid-type alkaloids with an unusual Δ<SUP>8</SUP>-7,12-lactam moiety, glechomanamides A-C (<B>1</B>-<B>3</B>), and two pairs of 7,12-hemiketal sesquiterpenoid epimers (<B>4a</B>/<B>b</B>, <B>5a</B>/<B>b</B>) were isolated from <I>Salvia scapiformis</I>. Their structures were elucidated by spectroscopic methods including HRESIMS, IR, UV, and 1D and 2D NMR and also confirmed by single-crystal X-ray diffraction analysis. The chemical transformation of compounds <B>1</B>-<B>5</B> in a solution environment was analyzed by 2D NMR spectroscopy. The aza acetallactams (<B>1</B>-<B>3</B>) were stable in organic solvent, while single crystals of the hemiacetal esters (<B>4a</B>/<B>b</B>, <B>5a</B>/<B>b</B>) underwent a tautomeric equilibrium after being dissolved. Single crystals of <B>4a</B>, <B>4b</B>, and <B>5a</B> were obtained for the first time as their naturally occurring forms. Glechomanamide B (<B>2</B>) exhibited antiangiogenic activity by suppression of vascular endothelial growth factor (VEGF)-induced tube formation through modulation of VEGF receptor 2 (VEGFR2)-mediated signaling pathways in human umbilical vascular endothelial cells (HUVECs). In addition, compound <B>2</B> also showed the significant suppression of mRNA expression associated with glycolysis and angiogenesis biomarkers in high glucose (30 mM)-induced HUVECs. These findings suggest that compound <B>2</B> might be a potential lead compound candidate for the management of diabetic retinopathy.</P> [FIG OMISSION]</BR>

Genetic Characteristics and Phylogeographic Dynamics of Echovirus

Wang Yan,Shah Pir Tariq,Liu Yue,Bahoussi Amina Nawal,Xing Li 한국미생물학회 2023 The journal of microbiology Vol.61 No.9

Echoviruses belong to the genus Enterovirus in the Picornaviridae family, forming a large group of Enterovirus B (EVB) within the Enteroviruses. Previously, Echoviruses were classified based on the coding sequence of VP1. In this study, we performed a reliable phylogenetic classification of 277 sequences isolated from 1992 to 2019 based on the full-length genomes of Echovirus. In this report, phylogenetic, phylogeographic, recombination, and amino acid variability landscape analyses were performed to reveal the evolutional characteristics of Echovirus worldwide. Echoviruses were clustered into nine major clades, e.g., G1–G9. Phylogeographic analysis showed that branches G2–G9 were linked to common strains, while the branch G1 was only linked to G5. In contrast, strains E12, E14, and E16 clustered separately from their G3 and G7 clades respectively, and became a separate branch. In addition, we identified a total of 93 recombination events, where most of the events occurred within the VP1-VP4 coding regions. Analysis of amino acid variation showed high variability in the a positions of VP2, VP1, and VP3. This study updates the phylogenetic and phylogeographic information of Echovirus and indicates that extensive recombination and significant amino acid variation in the capsid proteins drove the emergence of new strains.

Overexpression of a maize MYB48 gene confers drought tolerance in transgenic arabidopsis plants

Yan Wang,Qianqian Wang,MingLi Liu,Chen Bo, Xi Wang,Qing Ma,Beijiu Cheng,Ronghao Cai 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6

MYBs are a vital family of transcription factorsthat play critical roles in plant development and stressresponse. However, knowledge concerning the functions ofMYBs in the non-model plants is still limited. In this study,we isolated a R1-type MYB gene from maize (Zea mays L.),designated as ZmMYB48. Quantitative RT-PCR analysisdemonstrated that ZmMYB48 expression was induced bydrought and ABA treatments. Subcellular localization analysisrevealed that ZmMYB48 protein was targeted to the nucleusin tobacco leaf epidermal cells. Transactivation assay in yeastdemonstrated that ZmMYB48 had transcriptional activationability. Heterologous overexpression of ZmMYB48 inArabidopsis remarkably improved plant tolerance to droughtstress, as determined through physiological analyses ofsurvival rate, relative water content, malonaldehyde content,relative electrolyte leakage and proline content. Moreover,overexpression of ZmMYB48 enhanced the expression ofstress/ABA-responsive genes such as P5CS1, RD22, RD29Band ABI1. In addition, ZmMYB48-overexpressing plantsaccumulated higher content of ABA than WT plants underdrought stress. These results demonstrate that ZmMYB48might act as a positive regulator that participates in thedrought stress response through ABA signalling.