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        • KCI등재

          Characterization of Glycerol Dehydrogenase from Thermoanaerobacterium thermosaccharolyticum DSM 571 and GGG Motif Identification

          ( Liangliang Wang ),( Jiajun Wang ),( Hao Shi ),( Huaxiang Gu ),( Yu Zhang ),( Xun Li ),( Fei Wang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6

          Glycerol dehydrogenases (GlyDHs) are essential for glycerol metabolism in vivo, catalyzing its reversible reduction to 1,3-dihydroxypropranone (DHA). The gldA gene encoding a putative GlyDH was cloned from Thermoanaerobacterium thermosaccharolyticum DSM 571 (TtGlyDH) and expressed in Escherichia coli. The presence of Mn(2+) enhanced its enzymatic activity by 79.5%. Three highly conserved residues (Asp(171), His(254), and His(271)) in TtGlyDH were associated with metal ion binding. Based on an investigation of glycerol oxidation and DHA reduction, TtGlyDH showed maximum activity towards glycerol at 60°C and pH 8.0 and towards DHA at 60°C and pH 6.0. DHA reduction was the dominant reaction, with a lower Km(DHA) of 1.08 ± 0.13 mM and Vmax of 0.0053 ± 0.0001 mM/s, compared with glycerol oxidation, with a Km(glycerol) of 30.29 ± 3.42 mM and Vmax of 0.042 ± 0.002 mM/s. TtGlyDH had an apparent activation energy of 312.94 kJ/mol. The recombinant TtGlyDH was thermostable, maintaining 65% of its activity after a 2-h incubation at 60°C. Molecular modeling and site-directed mutagenesis analyses demonstrated that TtGlyDH had an atypical dinucleotide binding motif (GGG motif) and a basic residue Arg(43), both related to dinucleotide binding.

        • SCIESCOPUSKCI등재

          Characterization of Glycerol Dehydrogenase from Thermoanaerobacterium thermosaccharolyticum DSM 571 and GGG Motif Identification

          Wang, Liangliang,Wang, Jiajun,Shi, Hao,Gu, Huaxiang,Zhang, Yu,Li, Xun,Wang, Fei The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.6

          Glycerol dehydrogenases (GlyDHs) are essential for glycerol metabolism in vivo, catalyzing its reversible reduction to 1,3-dihydroxypropranone (DHA). The gldA gene encoding a putative GlyDH was cloned from Thermoanaerobacterium thermosaccharolyticum DSM 571 (TtGlyDH) and expressed in Escherichia coli. The presence of Mn<sup>2+</sup> enhanced its enzymatic activity by 79.5%. Three highly conserved residues (Asp<sup>171</sup>, His<sup>254</sup>, and His<sup>271</sup>) in TtGlyDH were associated with metal ion binding. Based on an investigation of glycerol oxidation and DHA reduction, TtGlyDH showed maximum activity towards glycerol at 60℃ and pH 8.0 and towards DHA at 60℃ and pH 6.0. DHA reduction was the dominant reaction, with a lower K<sub>m(DHA)</sub> of 1.08 ± 0.13 mM and V<sub>max</sub> of 0.0053 ± 0.0001 mM/s, compared with glycerol oxidation, with a K<sub>m(glycerol)</sub> of 30.29 ± 3.42 mM and V<sub>max</sub> of 0.042 ± 0.002 mM/s. TtGlyDH had an apparent activation energy of 312.94 kJ/mol. The recombinant TtGlyDH was thermostable, maintaining 65% of its activity after a 2-h incubation at 60℃. Molecular modeling and site-directed mutagenesis analyses demonstrated that TtGlyDH had an atypical dinucleotide binding motif (GGG motif) and a basic residue Arg<sup>43</sup>, both related to dinucleotide binding.

        • KCI등재

          RCS Numerical Simulation of Stealth Modified Three-Surface Aircraft

          Cheng Liangliang,Yue Kuizhi,Xing CuiFang,Yu Dazhao 한국항공우주학회 2016 International Journal of Aeronautical and Space Sc Vol.17 No.1

          The RCS characteristics of stealth modified three-surface aircraft are analyzed in this paper. Prototype A is built with CATIA software and the three-dimensional digital models of modified stealth three-surface B and C are also designed based on carrier-based aircraft Su-33; the numerical simulation of RCS characteristics of three-surface aircraft is conducted with RCSAnsys software based on physical optics method and the method of equivalent currents; The following results are obtained by comparative analysis and mathematical statistics: (1) by the use of physical optics method and equivalent electromagnetic current method, the scattering intensity for each part of the model and RCS characteristic of the aircraft can be analyzed efficiently and accurately; (2) compared with model A, the mean RCS value of model B is reduced to 14.1% in forward direction and 48.1% in lateral direction; (3) compared with model A, the mean RCS value of model C decreases to 11.4% in forward direction and 21.6% in lateral direction. The results are expected to provide theoretical basis and technical support to the conceptual design of aircraft and stealth technology research.

        • KCI등재

          Deformation research of Silty Clay Under Freeze-Thaw Cycles

          Lihui Tian,Liangliang Yu,Shiming Liu,Bo Zhang 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.2

          To explore the dynamic properties of silty clay in seasonally frozen ground under repeated freeze-thaw cycles, an unconsolidated undrained dynamic triaxial test was conducted on such properties under various confining pressures, number of freeze-thaw cycles, and dynamic stress amplitudes. The test results showed that due to the impact of freeze-thaw cycles, the inherent structure of the soil underwent some major changes, in which the resilient strain increased and the resilient modulus decreased. However, as the number of freeze-thaw cycles increased, the effect was reduced and eventually tended to be stable. As the number of freeze-thaw cycles increased, the cumulative plastic strain of the soil rose rapidly, and the soil samples became more prone to fracture at low stress amplitudes and small number of vibrations. The more the number of freeze-thaw cycles, the higher the cumulative plastic strain rate generated under a single dynamic stress amplitude. Moreover, with the increase in the confining pressure, the failure form of the thawed silty clay gradually evolved from a plastic fracture to a brittle one. The research results of this paper were helpful to further understand the influence of freeze-thaw cycle on the dynamic properties of silty clay, and provide data support for the subsequent research on the mechanism of roadbed diseases under the load in seasonally frozen ground.

        • KCI등재

          Dynamic response of a base-isolated CRLSS with baffle

          Xuansheng Cheng,Bo Liu,Liangliang Cao,Dongpo Yu,Huan Feng 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.66 No.3

          Although a rubber isolation cushion can reduce the dynamic response of a structure itself, it has little influence on the height of a sloshing wave and even may induce magnification action. Vertical baffles are set into a base-isolated Concrete Rectangular Liquid Storage Structure (CRLSS), and baffles are opened as holes to increase the energy dissipation of the damping. Problems of liquid nonlinear motion caused by baffles are described using the Navier-Stokes equation, and the space model of CRLSS is established considering the Fluid-Solid Interaction (FSI) based on the Finite Element Method (FEM). The dynamic response of an isolated CRLSS with various baffles under an earthquake is analyzed, and the results are compared. The results show that when the baffle number is certain, the greater the number of holes in baffles, the worse the damping effects; when a single baffle with holes is set in juxtaposition and double baffles with holes are formed, although some of the dynamic response will slightly increase, the wallboard strain and the height of the sloshing wave evidently decrease. A configuration with fewer holes in the baffles and a greater number of baffles is more helpful to prevent the occurrence of two failure modes: wallboard leakage and excessive sloshing height.

        • KCI등재

          Nodule Rich Protein 2 modulates nodule number in Medicago truncatula

          Junhui Yan,Xinwei Yang,Yawen Wang,Liangliang Yu,Li Luo 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.1

          Symbiotic nitrogen fxation is beneft to sustainable agriculture and global nitrogen cycle. Many small peptides were identifed as regulators involving in the interaction between rhizobia and legume. Here we reported Nodule Rich Protein 2 (MtNRP2) encoding a small peptide in Medicago truncatula, belonged to a group of nodule rich protein restricted in legume species. MtNRP2 expressed highly in root nodule and its promoter was active during the initiation and development of root nodule and lateral root. To investigate the function of MtNRP2 in nodulation, we generated MtNRP2-overexpression and MtNRP2- knockdown transgenic Medicago. MtNRP2-overexpression transgenic lines performed normal nodulation phenotype compared with vector control. However, in the MtNRP2-RNAi transgenic plants, the decrease of MtNRP2 expression lead to the increase of infection threads number (7 day post inoculation) and nodules number (3 week post inoculation); meanwhile, the expression of MtRGF3 and MtPUB1 was inhibited. These results suggested that MtNRP2 negatively regulated nodulation in Medicago truncatula.

        • SCIESCOPUSKCI등재후보

          Dynamic response of a base-isolated CRLSS with baffle

          Cheng, Xuansheng,Liu, Bo,Cao, Liangliang,Yu, Dongpo,Feng, Huan Techno-Press 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.66 No.3

          Although a rubber isolation cushion can reduce the dynamic response of a structure itself, it has little influence on the height of a sloshing wave and even may induce magnification action. Vertical baffles are set into a base-isolated Concrete Rectangular Liquid Storage Structure (CRLSS), and baffles are opened as holes to increase the energy dissipation of the damping. Problems of liquid nonlinear motion caused by baffles are described using the Navier-Stokes equation, and the space model of CRLSS is established considering the Fluid-Solid Interaction (FSI) based on the Finite Element Method (FEM). The dynamic response of an isolated CRLSS with various baffles under an earthquake is analyzed, and the results are compared. The results show that when the baffle number is certain, the greater the number of holes in baffles, the worse the damping effects; when a single baffle with holes is set in juxtaposition and double baffles with holes are formed, although some of the dynamic response will slightly increase, the wallboard strain and the height of the sloshing wave evidently decrease. A configuration with fewer holes in the baffles and a greater number of baffles is more helpful to prevent the occurrence of two failure modes: wallboard leakage and excessive sloshing height.

        • KCI등재

          Density functional theory study on Hg removal mechanisms of Cu-impregnated activated carbon prepared by simplified method

          Yuqun Zhuo,Yaming Fan,Zhenwu Zhu,Liangliang Li,Qun Chen,Yu Lou 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.10

          The preparation of activated carbon sorbent for Hg removal was simplified by combining activation and functionalization processes into one step. Jujube-based carbon material was first mixed with CuCl2 solution and then activated for the preparation of Cu-impregnated activated carbon. Physical and chemical properties of prepared activated carbon were investigated by means of N2 adsorption, SEM-EDS, XRD. A fixed-bed reactor with CEMS (Continuous emission monitoring system) was used to test the Hg adsorption ability of prepared activated carbon. DFT (Density functional theory) method of computational chemistry calculation was applied to identify the Hg adsorption mechanisms on sorbent surface.

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