Effects of chlorantraniliprole and chromafenozide on mortality and feeding cessation of the fall webworm, Hyphantria cunea (Lepidoptera: Arctiidae)

Ya-Jun Gong,Jin-Cui Chen,Shao-Kun Guo,Pan Shi,Li-Jun Cao,Ming-Liang Li,Ary A. Hoffmann,Shu-Jun Wei 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

The fall webworm (FWW) Hyphantria cunea, native to North America, is a globally invasive pest of a wide range of forest and fruit trees. Spraying of pesticides is the primary method for the control of FWW. In this study, toxicity and feeding cessation of two potential pesticides against the FWW, chlorantraniliprole, and chromafenozide, were evaluated. Both pesticides were slow to affect FWW. For chlorantraniliprole, the highest mortality of third instar larvae occurred at 72 h with an LC 50 of 10.34 mg/L, while for chromafenozide, the highest mortality occurred at 72 h with an LC 50 value 74.0950 mg/L. Low concentrations of both pesticides led to larvae ceasing to feed after six hours (chlorantraniliprole) and 24 h (chromafenozide). Both pesticides had persistent effects; thirty days after being applied at concentrations of 16, 26.67, and 35.56 mg/L to leaves, 93.33% of newly contacted larvae died after seven days. Our study showed that chlorantraniliprole and chromafenozide could be alternatively used against FWW and form a component of integrated control programs. The results provide information to guide the usage of chlorantraniliprole and chromafenozide in FWW control.

Effects of tryptophan and phenylalanine on tryptophol production in Saccharomyces cerevisiae revealed by transcriptomic and metabolomic analyses

Gong Xiaowei,Luo Huajun,Hong Liu,Wu Jun,Wu Heng,Song Chunxia,Zhao Wei,Han Yi,Dao Ya,Zhang Xia,Zhu Donglai,Luo Yiyong 한국미생물학회 2022 The journal of microbiology Vol.60 No.8

Tryptophol (TOL) is a metabolic derivative of tryptophan (Trp) and shows pleiotropic effects in humans, plants and microbes. In this study, the effect of Trp and phenylalanine (Phe) on TOL production in Saccharomyces cerevisiae was determined, and a systematic interpretation of TOL accumulation was offered by transcriptomic and metabolomic analyses. Trp significantly promoted TOL production, but the output plateaued (231.02−266.31 mg/L) at Trp concentrations ≥ 0.6 g/L. In contrast, Phe reduced the stimulatory effect of Trp, which was strongly dependent on the Phe concentration. An integrated genomic, transcriptomic, and metabolomic analysis revealed that the effect of Trp and Phe on TOL production was mainly related to the transamination and decarboxylation of the Ehrlich pathway. Additionally, other genes, including thiamine regulon genes (this), the allantoin catabolic genes dal1, dal2, dal4, and the transcriptional activator gene aro80, may play important roles. These findings were partly supported by the fact that the thi4 gene was involved in TOL production, as shown by heterologous expression analysis. To the best of our knowledge, this novel biological function of thi4 in S. cerevisiae is reported here for the first time. Overall, our findings provide insights into the mechanism of TOL production, which will contribute to TOL production using metabolic engineering strategies.

A Novel Leader Following Consensus Approach for Multi-agent Systems with Data Loss

Xiang Gong,Ya-Jun Pan,Ajinkya Pawar 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.2

The objective of this paper is to develop a novel consensus algorithm or protocol for multi-agent systemsin the event of communication link failure over the network. The structure or topology of the system is modeledby an algebraic graph theory, and considered as a discrete time-invariant system with a second-order dynamics. Bernoulli distribution is applied to represent the data dropout during operation. The sufficient conditions for thestabilization controller design is developed by Lyapunov-based methodologies and Linear matrix inequality (LMIs)techniques. The feasibility of the given LMIs is analyzed to verify the stabilization of controller design, whichensures the MAS to achieve the consensus. Leader-following numerical simulations with a group of agents aresuccessfully conducted based on the effect of data losses, initial values, communication weights, and number ofagents to demonstrate the effectiveness of the novel consensus algorithm in this paper. Finally, experimental studiesare carried out by using two Pioneer 3-DX mobile robots and one Pioneer 3-AT mobile robot as a team to verify theproposed work.

UV-Vis Spectra of N,N`-Bis (4`-Aminophenyl)-1,4-quinonenediimine Doped with H₄SiW₁₂O₄₀

Jian Gong,Xiu Jun Cui,Ya Guang Chen,Zhong Wei Xie,Lun Yu Qu 한국고분자학회 2004 Macromolecular Research Vol.12 No.1

Laboratory selection for resistance to sulfoxaflor and fitness costs in the green peach aphid Myzus persicae

Shinji Sugiur,Ya-Jun Gong,Jin-Cui Chen,Xiao-Chun Su,Li-Jun Cao,Ary Anthony Hoffmann,Shu-Jun Wei 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

Sulfoxaflor is a newly released fourth-generation neonicotinoid insecticide for management of sap-feeding peststhat have developed resistance to established insecticide groups. The risk of resistance developing to this pesticidein target pests is unclear. We selected a strain of the green peach aphid, Myzus persicae (Sulzer)(Hemiptera: Aphididae), for resistance to sulfoxaflor in the laboratory, which showed 199-fold resistance after45 generations compared to the starting population. Life table analysis showed that the resistant strain had afitness of 0.83 compared to the susceptible strain. Adult longevity of the resistant strain was reduced by 9.55%compared to the susceptible strain. The period when adults of the resistant strain produced offspring was reducedby 17.19%, while the mean fecundity of the resistant strain was reduced by 15%. These findings suggestthat M. persicae can develop a high level of resistance to sulfoxaflor, but fitness costs may result in a recovery ofsensitivity when field populations are no longer exposed to sulfoxaflor.

Adherence to Cancer Prevention Guidelines and Endometrial Cancer Risk: Evidence from a Systematic Review and Dose-Response Meta-analysis of Prospective Studies

Hui Sun,Qing Chang,Ya-Shu Liu,Yu-Ting Jiang,Ting-Ting Gong,Xiao-Xin Ma,Yu-Hong Zhao,Qi-Jun Wu 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1

Purpose The evidence of adherence to cancer prevention guidelines and endometrial cancer (EC) risk has been limited and controversial. This study summarizes and quantifies the relationship between adherence to cancer prevention guidelines and EC risk. Materials and Methods The online databases PubMed, Web of Science, and EMBASE were searched for relevant publications up to June 2, 2020. This study had been registered at PROSPERO. The registration number is CRD42020149966. Study quality evaluation was performed based on the Newcastle-Ottawa Scale. The I2 statistic was used to estimate heterogeneity among studies. Egger’s and Begg’s tests assessed potential publication bias. Summary hazard ratios (HRs) and 95% confi dence intervals (CIs) for the relationship between adherence to cancer prevention guidelines score was assigned to participants by summarizing individual scores for each lifestyle-related factor. The scores ranged from least healthy (0) to most healthy (20) and the EC risk was calculated using a random-effects model. Results Five prospective studies (four cohort studies and one case‑cohort study) consisted of 4,470 EC cases, where 597,047 participants were included. Four studies had a low bias risk and one study had a high bias risk. Summary EC HR for the highest vs. lowest score of adherence to cancer prevention guidelines was 0.54 (95% CI, 0.40 to 0.73) and had a high heterogeneity (I2=86.1%). For the dose-response analysis, an increment of 1 significantly reduced the risk of EC by 6%. No signifi cant publication bias was detected. Conclusion This study suggested that adherence to cancer prevention guidelines was negatively related to EC risk.

Next-generation sequencing of the mitochondrial genome of Dolichovespula panda (Hymenoptera: Vespidae) with a phylogenetic analysis of Vespidae

Xu-Lei Fan,Ya-Jun Gong,Peng-Yan Chen,Qing-Qing Tan,Jiang-Li Tan,Shu-Jun Wei 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3

For the first time the mitochondrial genome of a Dolichovespula species, D. panda Archer (Hymenoptera: Vespidae), was sequenced with a next-generation sequencing approach. The sequenced mitochondrial genome is 17137 bp long and consists of 13 protein-coding, 22 tRNA and two rRNA genes, as well as a partial A +T-rich region. Twenty-two of the genes are encoded on the majority strand and 15 genes on the minority strand. All protein-coding genes start with ATN codons and have a TAA termination codon, except for one with a TA codon. Compared with the putative ancestral arrangement of insects, the D. panda mitochondrial genome shows the shuffling of trnN and trnE, and of trnQ and trnM, the translocation of trnY to upstream of trnI, and of trnL1 to the region between trnS2 and nad1 and a reversal of trnS1. A phylogenetic tree within the Vespidae was reconstructed using the 13 protein-coding mitochondrial genes. This shows a sister group relationship between Dolichovespula and a clade formed by Vespa and Vespula. It also corroborated the position of Eumeninae as sister group of the clade Polistinae +Vespinae.

Effect of temperature on the development of Echinothrips americanus Morgan (Thysanoptera: Thripidae) with special reference to the number of generations

Liang Zhu,Ze-Hua Wang,Ya-Jun Gong,Li-Jun Cao,Shu-Jun Wei 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

The thrips Echinothrips americanus Morgan (Thysanoptera: Thripidae) was recently reported in China. The effect of temperature on its development was investigated and the potential area of further dispersal was predicted by estimation of its generations in China. First instar larvae developed to adults on green peppers Capsicum anuum at all constant temperatures (16, 19, 22, 25, 28, 31 and 34 °C) except for 34 °C, at which they developed only as far as the second instar. The percentage surviving of immature stages were not significantly different at the other six temperatures (range 32.10%–52.72%). The developmental times from egg to adult ranged from 11.68 to 45.13 days and were negatively correlated with temperature. There were significant differences between male and female immature stages in their developmental time at 22 °C and 25 °C. Regression analysis indicated that both the Lactin and linear models could describe the relationship between developmental rate and temperature for immature stages but a slightly higher coefficient of determination was obtained with the Lactin model. The high-temperature threshold and the optimum temperature for development were 32.04 °C and 31.79 °C, respectively, estimated by the Lactin model. The low-temperature threshold for development was 11.35 °C and thermal constant was 229.76 degree-days for immature stages, estimated by the linear model. The models predicted that E. americanus could have produced 8–10 generations per year under field conditions in Beijing (N39°56′, E116°17′) and Yangling (N34°12′, E108°7′) from 2002 to 2012. The potential risk posed by E. americanus to crops in different regions of China was discussed.

Brain-based Correlations Between Psychological Factors and Functional Dyspepsia

( Jiao Fen Nan ),( Ji Xin Liu ),( Jun Ya Mu ),( Wang Huan Dun ),( Ming Zhang ),( Qiyong Gong ),( Wei Qin ),( Jie Tian ),( Fanrong Liang ),( Fang Zeng ) 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.1

Background/Aims Increasing evidence shows involvement of psychological disorders in functional dyspepsia (FD), but how psychological factors exert their influences upon FD remains largely unclear. The purpose of the present study was to explore the brain-based correlations of psychological factors and FD. Methods Based on Fluorine-18-deoxyglucose positron emission tomography-computed tomography, the altered cerebral glycometabolism was investigated in 40 FD patients compared with 20 healthy controls during resting state using statistical parametric mapping software. Results FD patients exhibited increased glucose metabolism in multiple regions relative to controls (P < 0.001, family-wise error corrected). After controlling for the dyspeptic symptoms, increased aberrations persisted within the insula, anterior cingulate cortex (ACC), middle cingulate cortex (MCC) and middle frontal cortex (midFC), which was related to anxiety and depression score. Interestingly, FD patients without anxiety/depression symptoms also showed increased glycometabolism within the insula, ACC, MCC and midFC. Moreover, FD patients with anxiety/depression symptoms exhibited more significant hypermetabolism within the above 4 sites compared with patients without anxiety/depression symptoms.Conclusions Our results suggested that the altered cerebral glycometabolism may be in a vicious cycle of psychological vulnerabilities and increased gastrointestinal symptoms.

Generation of Selenoprotein with Glutathione Peroxidase Activity by Chemical Modification of the Single-chain Variable Fragment Expressed in a Single-protein Production System and Its Antioxidant Ability

Cheng Wang,Gang-Lin Yan,Shao-Wu Lü,Chun-Hong Sui,Yang Zhao,Ya-Wei Xu,Gang Zhao,Jun-jie Xu,Ping-Sheng Gong,Gui-Min Luo,Ying Mu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1

Glutathione peroxidase (GPX) is one of the important members of the antioxidant enzyme family. It can catalyze the reduction of hydroperoxides with glutathione to protect cells against oxidative damage. Single-chain variable fragment (scFv) can be converted into seleniumcontaining single-chain variable fragment (Se-scFv) by chemical modification of the hydroxyl groups in scFv, thus Se-scFv possesses GPX activity and becomes a prodrug. To improve the expression of scFv and simplify its purification steps, Single-protein production (SPP) system was used to express scFv and chemical modification was used to synthesize Se-scFv. Therefore, we must construct a new scFv-WCD1-lessACA gene, which can express its mRNA not containing any ACA sequences and express its amino acid sequence of target protein (scFv) being same to scFv-WCD1. In this way, the scFv-WCD1-lessACA can be only expressed in SPP system and no other background proteins in the cells could be expressed. The expression results showed that high level of scFv-WCD1-lessACA synthesis was at least sustained for 96 h in the virtual absence of background protein synthesis. Then, selenocysteine (Sec) was incorporated into the scFv-WCD1-lessACA by chemical modification and resulted in Se-scFv-WCD1-lessACA. The enzymatic characteristics of Se-scFv-WCD1-lessACA were determined. GPX activity was 2,563 U/μmol,its binding constant for GSH was 0.687 ×105/mol. Moreover,Se-scFv-WCD1-lessACA was confirmed to have a strong antioxidant ability to protect mitochondria against oxidative damage induced by Vc/Fe2+ (mitochondrial damage model),suggesting that Se-scFv-WCD1-lessACA has potential application for protection of mitochondrial damage induced by reactive oxygen species (ROS).