Mutation Breeding of High Avermectin B1a-producing Strain by the Combination of High Energy Carbon Heavy Ion Irradiation and Sodium Nitrite Mutagenesis Based on High Throughput Screening

Xiaoqing Song,Yun Zhang,Xudong Zhu,Yonghong Wang,Ju Chu,Ying-ping Zhuang 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.5

Microbial mutation breeding has been widely used because it is one of the most efficient and practical breeding strategies in the fermentation industry. However, different mutagenesis methods cause various degrees of DNA damage to individual microorganisms, which lead to diverse characteristics of the mutants. In this study, the effects of four different mutagenesis methods on the mutation breeding of Streptomyces avermitilis for improving avermectin B1a production were investigated with an optimized liquid microtiter plate (MTP) culture system. First, an effective and feasible MTP system for mutant strain screening was evaluated through the optimization of the oxygen transfer rate and rapid titer determination. Then, high energy carbon heavy ion irradiation, diethyl sulfate, ultraviolet- (UV) irradiation combined with lithium chloride, and sodium nitrite were used as the mutagens for mutation breeding, respectively. Results showed that carbon heavy ion irradiation had the advantages of possessing the highest positive mutation rate and mean-production of positive mutant strains in the first generation. Sodium nitrite treatment resulted in mutant strains with better inherited stability than the other three methods. Through the combined treatment of carbon heavy ion irradiation and sodium nitrite treatment, an inheritstable mutant S. avermitilis S-233 with high avermectin B1a production was successfully obtained. The fermentation verification in a 500-liter (L) bioreactor demonstrated that the avermectin B1a produced by mutant S. avermitilis S-233 reached 6818 μg/mL, which was 23.8% higher than that of parent strains.

Multi-objective Integration of Flexible Collaborative Planning and Fuzzy Flexible Lot-Splitting Scheduling Based on the Pareto Optimal

Zhenqiang Bao,Xiaoqing Ren,Yulu Yang,Junwu Zhu,Cheng Wang,Richao Yin 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.7

Since that a job usually contains several working procedures in actual production, and it’s hard to optimize the flexible collaborative planning, the flexible lot splitting and scheduling of the job simultaneously in batch production mode, a model for multi-objective integration of flexible collaborative planning and fuzzy lot-splitting scheduling is established. We take four performance indicators below which are the most common as standards to optimize the model: average delivery satisfaction, fuzzy total cost, fuzzy completion time and average credibility of job tardiness, and then a multi-objective algorithm based on the Pareto optimal is established. In this algorithm, we design the integrated coding scheme, which include collaboration chromosome, lot-splitting chromosome and scheduling chromosome, meanwhile the Pareto optimal scheme is designed. Finally, the efficiency of the model and algorithm is proved by the simulation.

Preparation and Characterization of Regenerated Cellulose Blend Films Containing High Amount of Poly(vinyl alcohol) (PVA) in Ionic Liquid

Xiaomin Zhang,Jin Zhu,Xiaoqing Liu 한국고분자학회 2012 Macromolecular Research Vol.20 No.7

Regenerated cellulose/poly(vinyl alcohol) (PVA) blend films in ionic liquid 1-allyl-3-methylimidazolium chloride (AMIMCl) were successfully prepared by coagulating with water. The structures and properties were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and differential scanning calorimetry (DSC). SEM images indicated that there was good compatibility between cellulose and PVA. The XRD results showed that the crystallinity of cellulose was significantly decreased from 42.8% up to 29.4% with increasing PVA content. The blend film with a 10 wt% PVA demonstrated the best mechanical performance. Its tensile strength and modulus showed 97 and 5,100 MPa, respectively compared with 69 and 3,400 MPa of neat cellulose film. The elongation also increased 50%. In addition, the transparency of the blend lms was still maintained well, and the shrinkage of the regenerated cellulose film was greatly improved with incorporation of PVA. The possible mechanism of forming cellulose/PVA blend films was proposed in the process of regeneration and dissolution.

A Theoretical Study of Estimating the Elastic Responses of Framed Self-Centering Wall Structures under Lateral Loading

Xiaobin Hu,Chen Lu,Xiaoqing Zhu,Xiangdong Xie 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.12

The framed self-centering wall (FSCW) structure is a newly-emerged special type of seismic-resistant system. However, the related studies rarely concern the theoretical investigation of the FSCW structures subjected to lateral loading, which is of great importance for seismic design of the FSCW structures. In view of this, the analytical model of the FSCW structures is firstly developed, then the fundamental equations that govern the elastic responses of FSCW structures under lateral loads are formulated and the corresponding analytical solutions are derived. Meanwhile, the developed analytical model is validated by the finite element method, which shows that the developed model has a good reliability in predicting the elastic responses of FSCW structures sustaining lateral loading. Utilizing the proposed method, the effects of the post-tensioned (PT) tendon and dampers on the behaviors of the FSCW structure under lateral loading are investigated. Moreover, a comparative study of mechanical performance between the FSCW structure and conventional structures under lateral loading is conducted. The results show that, in terms of the lateral displacement and shear force responses of the frame, the mechanical performance of the FSCW structure subjected to lateral loading generally falls in between those of the conventional frame-shear wall structure and the moment-resisting frame structure.

Effect of Physical Activity on the Association Between Diet and Constipation: Evidence From the National Health and Nutrition Examination Survey 2007-2010

Shijun Lai,Changdong Zhu,Xiaoqing Zhou,Qingfeng Zeng,Lihua Huang,Xiaodong Cao,Qiang Zhou,Yuhua Zhong,Jinjing Huang,Guifang Zeng,Hong Chen 대한소화기 기능성질환∙운동학회 2024 Journal of Neurogastroenterology and Motility (JNM Vol.30 No.3

Background/AimsPrevious studies have shown that diet and physical activity can influence constipation. However, the combined effect of diet and physical activity on constipation remains unclear. MethodsConstipation was defined based on stool consistency and frequency, while overall diet quality was assessed using Healthy Eating Index (HEI)-2015 scores. Participants were categorized into low (metabolic equivalent [MET]-min/wk < 500) and high physical activity groups (MET-min/wk ≥ 500). The association between diet and constipation across physical activity groups was analyzed using survey logistic regression and restricted cubic splines. ResultsHigher HEI-2015 scores were associated with reduced constipation risk in the high physical activity group when constipation was defined by stool consistency (odds ratio [OR], 0.98; 95% confidence interval [CI], 0.97-0.99). However, in the low physical activity group, increased HEI-2015 scores did not significantly affect constipation risk (OR, 1.01; 95% CI, 0.97-1.05). Similar results were found when constipation was defined based on stool frequency. In the high physical activity group, increased HEI-2015 scores were significantly associated with a reduced constipation risk (OR, 0.96; 95% CI, 0.94-0.98). Conversely, in the low physical activity group, increased HEI-2015 scores did not affect the risk of constipation (OR, 0.96; 95% CI, 0.90-1.03). ConclusionsOur findings suggest that a higher HEI-2015 score is negatively associated with constipation among individuals with high physical activity levels but not among those with low physical activity levels. This association was consistent when different definitions of constipation were used. These results highlight the importance of combining healthy diet with regular physical activity to alleviate constipation.

Effects of Chicory Inulin on Serum Metabolites of Uric Acid, Lipids, Glucose, and Abdominal Fat Deposition in Quails Induced by Purine-Rich Diets

Zhijian Lin,Bing Zhang,Xiaoqing Liu,Rui Jin,Wenjing Zhu 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.11

Inulin, a group of dietary fibers, is reported to improve the metabolic disorders. In the present study, we investigated the effects of chicory inulin on serum metabolites of uric acid (UA), lipids, glucose, and abdominal fat deposition in quail model induced by a purine-rich diet. In this study, 60 male French quails were randomly allocated to five groups: CON (control group), MOD (model group), BEN (benzbromarone-treated group), CHI-H (high-dosage chicory inulin-treated group), and CHI-L (low-dosage chicory inulin-treated group). The serum UA level was significantly increased in the model group from days 7 to 28, as well as triglyceride (TG) and free fatty acid (FFA) increased later in the experimental period. The abdominal fat ratio was increased on day 28. Benzbromarone can decrease UA levels on days 14 and 28. The high and low dosage of chicory inulin also decreased serum UA levels on days 7, 14, and 28. The abdominal fat ratio, activity, and protein of acetyl-CoA carboxylase (ACC) were decreased in chicory inulin-treated groups. The activities of xanthine oxidase (XOD) and fatty acid synthase (FAS) were increased in the model group and decreased in the benzbromarone and chicory inulin groups. This study evaluated a quail model of induced hyperuricemia with other metabolic disorders caused by a high-purine diet. The results indicated that a purine-rich diet might contribute to the development of hyperuricemia, hypertriglyceridemia, and abdominal obesity. Chicory inulin decreased serum UA, TG, and abdominal fat deposition in a quail model of hyperuricemia by altering the ACC protein expression and FAS and XOD activities.

Decreased vitamin D-binding protein level portends poor outcome in acute-on-chronic liver failure caused by hepatitis B virus

Daxian Wu,Qunfang Rao,Zhongyang Xie,Xiaoqing Zhu,Jian Wu,Hainv Gao,Jingyu Zhang,Zhouhua Hou,Xiaoyu Cheng,Zeyu Sun 대한간학회 2022 Clinical and Molecular Hepatology(대한간학회지) Vol.28 No.4

Background/Aims: Acute-on-chronic liver failure (ACLF) is a catastrophic illness. Few studies investigated the prognostic value of vitamin D-binding protein (VDBP) for hepatitis B virus (HBV)-related ACLF (HBV-ACLF) resulted in conflicting results. Methods: Two prospective HBV-ACLF cohorts (n=287 and n=119) were enrolled to assess and validate the prognostic performance of VDBP. Results: VDBP levels in the non-survivors were significantly lower than in the survivors (P<0.001). Multivariate Cox regression demonstrated that VDBP was an independent prognostic factor for HBV-ACLF. The VDBP level at admission gradually decreased as the number of failed organs increased (P<0.001), and it was closely related to coagulation failure. The areas under the receiver operating characteristic curve (AUCs) of the Child-Pugh-VDBP and chronic liver failuresequential organ failure assessment (CLIF–SOFA)-VDBP scores were significantly higher than those of Child-Pugh (P<0.001) and CLIF-SOFA (P=0.0013). The AUCs of model for end-stage liver disease (MELD)-VDBP were significantly higher than those of MELD (P= 0.0384) only in the case of cirrhotic HBV-ACLF patients. Similar results were validated using an external multicenter HBV-ACLF cohort. By longitudinal observation, the VDBP levels gradually increased in survivors (P=0.026) and gradually decreased in non-survivors (P<0.001). Additionally, the VDBP levels were found to be significantly decreased in the deterioration group (P=0.012) and tended to be decreased in the fluctuation group (P=0.055). In contrast, they showed a significant increase in the improvement group (P=0.036). Conclusions: The VDBP was a promising prognostic biomarker for HBV-ACLF. Sequential measurement of circulating VDBP shows value for the monitoring of ACLF progression.

Self-bending extrusion molding of distorted channels

Fanlei Min,Huiping Liu,Guangming Zhu,Zheng Chang,Xujie Gao,Bowen Yue,Nana Guo,Xiaoqing Zhai 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.5

Using an integrated profile extrusion and bending forming process with a streamlined extrusion die, a new self-bending extrusion molding technology is proposed with an axis-distorted variable channel. By designing the streamlined extrusion die structure of the distorted central axis, the metal was made to flow non-uniformly in the die cavity, thereby directly extruding a bent profile. The central axis of the streamlined extrusion die is described by a trigonometric function and a Gaussian function. A numerical simulation was applied to analyze the metal flow pattern, equivalent strain, and strain-rate distribution during the self-bending extrusion process. The influences of the extrusion velocity and the addition of a bearing on the self-bending deformation profiles were investigated. During the extrusion process, the streamline at the center of the billet could describe the overall flow of the metal in the die cavity, and the distance between the point on the end face of the die outlet and the center of the die outlet directly determined the degree of extrusion and bending. The greater the distance was, the larger was the degree of bending. The metal strain on the convex edge of the die was greater than that on the concave edge of the die, with the extruded profile always bending toward the concave edge. The strain rate of the metal changed the fastest near the most convex point of the die. As the extrusion velocity increased or more bearings were added, the radius of curvature of the extruded profile increased nonlinearly.

Depression and Structural Factors Are Associated With Symptoms in Patients of Irritable Bowel Syndrome With Diarrhea

( Jia Lu ),( Lili Shi ),( Dan Huang ),( Wenjuan Fan ),( Xiaoqing Li ),( Liming Zhu ),( Jing Wei ),( Xiucai Fang ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2020 Journal of Neurogastroenterology and Motility (JNM Vol.26 No.4

Background/Aims A strong correlation between depression and irritable bowel syndrome with diarrhea (IBS-D) has been identified. The aim of this study is to identify the correlations among depression, structural factors, gastrointestinal (GI) and extra-GI symptoms, and efficacy of neuromodulators in patients with IBS-D. Methods Patients meeting the Rome III Diagnostic Criteria for IBS-D were enrolled. The intestinal symptoms and psychological states were evaluated using IBS-specific symptom questionnaires and Hamilton Depression Rating Scale. Results In total, 410 patients with IBS-D were enrolled, 28.8% (118/410) had comorbid depression. Patients with depression did not readily experience improvement in abdominal pain/discomfort after defecation, and had a higher prevalence of passing mucus, overlapping functional dyspepsia, and extra-GI symptoms. The structural factor “mental disorders” significantly correlated with main bowel symptom score and degree of pre-defecation abdominal pain/discomfort. No structural factor significantly correlated with bowel movements or stool form. Patients who had passing mucus, overlapping functional dyspepsia and extra-GI painful symptoms have higher score of “anxiety/somatization.” Patients with sexual dysfunction have higher score of “retardation symptoms.” In total, 28.3% of patients with IBS-D were prescribed neuromodulators. Baseline scores of “anxiety/somatization” and “retardation symptoms” positively correlated with improvement of diarrhea after paroxetine, and “sleep disturbances” positively correlated with improvement of abdominal pain/discomfort and diarrhea after mirtazapine. Conclusions Comorbid depression and higher scores of structural factors might aggravate GI and extra-GI symptoms other than bowel movements and stool form. Structural factors of Hamilton Depression Rating Scale correlated with efficacy of paroxetine and mirtazapine in patients with IBS-D. (J Neurogastroenterol Motil 2020;26:505-513)