Statistical experimental design optimization of rhamsan gum production by Sphingomonas sp. CGMCC 6833

Xiao-Ying Xu,Shu-Hao Dong,Sha Li,Xiao-Ye Chen,Ding Wu,Hong Xu 한국미생물학회 2015 The journal of microbiology Vol.53 No.4

Rhamsan gum is a type of water-soluble exopolysaccharideproduced by species of Sphingomonas bacteria. The optimalfermentation medium for rhamsan gum production bySphingomonas sp. CGMCC 6833 was explored definition. Single-factor experiments indicate that glucose, soybean meal,K2HPO4 and MnSO4 compose the optimal medium alongwith and initial pH 7.5. To discover ideal cultural conditionsfor rhamsan gum production in a shake flask culture, responsesurface methodology was employed, from which thefollowing optimal ratio was derived: 5.38 g/L soybean meal,5.71 g/L K2HPO4 and 0.32 g/L MnSO4. Under ideal fermentationrhamsan gum yield reached 19.58 g/L ± 1.23 g/L,42.09% higher than that of the initial medium (13.78 g/L ±1.38 g/L). Optimizing the fermentation medium results inenhanced rhamsan gum production.

Ginsenoside Rg3 in combination with artesunate overcomes sorafenib resistance in hepatoma cell and mouse models

Ying-Jie Chen,Jia-Ying Wu,Yu-Yi Deng,Ying Wu,Xiao-Qi Wang,Amy Sze-man Li,Lut Yi Wong,Xiuqiong Fu,Zhi-Ling Yu,Chun Liang 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.3

Sorafenib is effective in treating hepatoma, but most patients develop resistance to it. STAT3signaling has been implicated in sorafenib resistance. Artesunate (ART) and 20(R)-ginsenoside Rg3 (Rg3)have anti-hepatoma effects and can inhibit STAT3 signaling in cancer cells. This study aimed to evaluatethe effects of Rg3 in combination with ART (Rg3-plus-ART) in overcoming sorafenib resistance, and toexamine the involvement of STAT3 signaling in these effects. Methods: Sorafenib-resistant HepG2 cells (HepG2-SR) were used to evaluate the in vitro anti-hepatomaeffects of Rg3-plus-ART. A HepG2-SR hepatoma-bearing BALB/c-nu/nu mouse model was used to assessthe in vivo anti-hepatoma effects of Rg3-plus-ART. CCK-8 assays and Annexin V-FITC/PI double stainingwere used to examine cell proliferation and apoptosis, respectively. Immunoblotting was employed toexamine protein levels. ROS generation was examined by measuring DCF-DA fluorescence. Results: Rg3-plus-ART synergistically reduced viability of, and evoked apoptosis in HepG2-SR cells, andsuppressed HepG2-SR tumor growth in mice. Mechanistic studies revealed that Rg3-plus-ART inhibitedactivation/phosphorylation of Src and STAT3 in HepG2-SR cultures and tumors. The combination alsodecreased the STAT3 nuclear level and induced ROS production in HepG2-SR cultures. Furthermore, overactivation of STAT3 or removal of ROS diminished the anti-proliferative effects of Rg3-plus-ART, andremoval of ROS diminished Rg3-plus-ART's inhibitory effects on STAT3 activation in HepG2-SR cells. Conclusions: Rg3-plus-ART overcomes sorafenib resistance in experimental models, and inhibition of Src/STAT3 signaling and modulation of ROS/STAT3 signaling contribute to the underlying mechanisms. Thisstudy provides a pharmacological basis for developing Rg3-plus-ART into a novel modality for treatingsorafenib-resistant hepatoma.

The ERCC1 C118T Polymorphism Predicts Clinical Outcomes of Colorectal Cancer Patients Receiving Oxaliplatin-Based Chemotherapy: a Meta-analysis Based on 22 Studies

Qian, Ying-Ying,Liu, Xin-You,Wu, Qian,Song, Xian,Chen, Xiao-Feng,Liu, Yi-Qian,Pei, Dong,Shen, Li-Zong,Shu, Yong-Qian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: Although the predictive value of the excision repair cross-complementing group 1 (ERCC1) C118T polymorphism in clinical outcomes of patients with colorectal cancer (CRC) receiving oxaliplatin-based chemotherapy has been evaluated in numerous published studies, the conclusions are conflicting. Therefore, we performed the present meta-analysis to determine the precise role of the ERCC1 C118T polymorphism in this clinical situation and help optimize individual chemotherapy. Materials and Methods: A multiple search strategy was used to identify eligible studies. Pooled odds ratios (ORs) and their 95% confidence intervals (CIs) were used to estimate objective response and oxaliplatin-induced toxicity, with hazard ratios (HRs) with 95%CIs for progression-free survival (PFS) and overall survival (OS). Results: A total of 22 studies including 2,846 CRC patients were eligible in the analysis. Overall, no significant correlation was found between the ERCC1 C118T polymorphism and objective response to oxaliplatin-based chemotherapy, in all patients or in the Asian and Caucasian subgroups. However, the pooled analysis showed that the PFS and OS were significantly shorter in patients who carried T/T or T/C genotypes of ERCC1 C118T as compared to the C/C genotype. On stratified analysis by ethnicity, the ERCC1 118T allele was associated with a favorable prognosis in Caucasians (PFS, HR=0.58, 95%CI: 0.24-1.44; OS, HR=0.38, 95%CI: 0.22-0.64) but an unfavorable prognosis in Asians (PFS, HR=2.49, 95%CI: 1.87-3.33; OS, HR=2.63, 95%CI: 1.87-3.69) based on a dominant model. In addition, we failed to find a statistically significant impact of ERCC1 C118T polymorphism on oxaliplatin-induced toxicity. Conclusions: The ERCC1 C118T polymorphism may have prognostic value in patients with CRC undergoing oxaliplatin-based chemotherapy.

Experimental study of natural convection heat transfer from a nonuniformly heated flat plate simulating PV panel

Shuang-Ying Wu,Ying-Ying Wu,Lan Xiao,Zhen Yang 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.1

In view of the characteristic of Photovoltaic (PV) conversion, an experimental study has been conducted to investigate the natural convection heat transfer from a flat plate. In order to simulate the real PV cells, three electrical heating circuits were employed to achieve a linear nonuniform heat flux boundary condition. The major parameters, such as the gradient parameter of heat flux k, tilt angle θ and heat flux q w were introduced to analyze their influence on heat transfer ability. Both the local temperature distribution and the overall trend of Nusselt number have been presented. Comparing with the uniform thermal boundary condition, the results show that the local convection heat transfer coefficient is fluctuated at the positions where heat flux has changed. When gradient parameter of heat flux increases, the difference of local convection heat transfer coefficient at the top of the plate between tilt angle θ = 0° and 90° becomes smaller. Moreover, it is observed that the gradient parameter of heat flux has a promotion effect on average convection Nusselt number at larger tilt angle, but the effect becomes complex as tilt angle is smaller. Finally, a correlation combining all significant factors has been put forward to estimate the natural convection heat transfer.

Effect of thermal radiation on convection heat transfer in cooling channel of photovoltaic thermal system

Shuang-Ying Wu,Ying-Ying Wu,Lan Xiao 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.8

The effect of thermal radiation on convection heat transfer in flat-box type cooling channel of photovoltaic thermal system with tilt angleof 30 degree was studied by 3D numerical simulation under constant heat flux boundary condition. The temperature contours andvelocity fields of fluid near the outlet were obtained. The variations of wall temperature and convection Nusselt number along flow directionfor all the separate walls composing the cooling channel were compared and analyzed. The results show that due to thermal radiation,the deflection of the maximum velocity region to heated top wall, together with the asymmetry of temperature field is weakened. Fornatural convection, radiation promotes the formation of multi-vortices. For mixed convection, heat transfer on all the cooling channelwalls is enhanced under the condition of lower heat flux while heat transfer on heated top wall is deteriorated when the heat flux is relativehigh. Also, pressure re-rising is promoted by thermal radiation.

β-catenin protein utilized by Tumour necrosis factor-α in porcine preadipocytes to suppress differentiation

( Xiao Luo ),( Hui Xia Li ),( Rong Xin Liu ),( Zong Song Wu ),( Ying Juan Yang ),( Gong She Yang ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.6

Metabolic Syndrome and Life Style in China

Wu Pei-Ying,Song Xiao-Min,Jin Qi-Lin,Wang Xin-Qiao,Wang Ai-Rong The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.3

The purpose of this study was to explore the relationship between life style and metabolic syndrome. The cross-sectional survey was conducted in Pingliang community in Shanghai in Jan 2003. The data was collected by questionnaire, and the results were analyzed by SPSS. It was found that the prevalence of Metabolic Syndrome (MS) was $13.4\%$ in the community, and the body mass index (BMI), waist-to-hip ratio (WHR), systolic blood pressure (SBP), diastolic blood pressure (DBP), serum triglyceride (TG), total cholesterol(TC), low density lipoprotein-cholesterol (LDL-C), and fast plasma glucose (FPG) in MS group were higher than that in non-MS group. Logistic regression analysis indicated that BMI and WHR were positively correlated to the prevalence of MS, and physical activity was negatively correlated to the prevalence of MS. People with higher education levels (${\geq}10y$) had lower BMI, SBP, DBP, LDL-C and FPG. The prevalence of MS in the higher education level group was significantly lower than that of the lower education level group. These results suggested that BMI, WHR and physical activity were important factors of MS, and education background played an important role in the occurrence of MS. Therefore, it is very important to build a healthy life style for preventing and controlling the incidence and developing of MS.

Early Diet Dilution with 40% Rice Hull Induces Lower Body Fat and Lipid Metabolic Programming in Peking Ducks

Xiao Yang Guo,Yong Jun Fang,Ling Ying Wu 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.3

This study was conducted to evaluate the effect of early diet dilution with 40% rice hull on growth performance, carcass characteristic and composition of meat-type ducks, and to reveal the possible mechanism for decreased body fat deposition. 160 1-day-old White Peking ducks with initial body weight of 44.5±1.0 g were allotted to two treatments with 8 replicate pens per treatment and 10 ducks per pen (5 male and 5 female). Ducks were fed with the experimental starter diets diluted with 0% (control, RH0), 40% rice hull (RH40) during 8 to 14 d of age, respectively. Thereafter, all ducks were fed with grower diet. Ducks fed with RH40 diet from 8 to 14 d of age increased (p<0.05) feed intake, decreased (p<0.05) body weight, body weight gain and adjusted feed intake (excluded rice hull), abdominal fat, skin with fat, and fat content in car-cass, and reduced (p<0.05) activities of hepatic malic dehydrogenase, glucose-6-phosphate dehydrogenase and fatty acid synthetase. When diet dilution was withdrawn in the re-fed period from 15 to 42 d of age, full compensatory growth of body weight, breast meat and leg meat weight were attained. However, ducks were still less (p<0.05) carcass fat content and showed continually lower (p<0.05) hepatic lipogenic enzyme activities at the market age in RH40 ducks than the control. These results indicated that diluting diet with 40% rice hull during 8 to 14 d of age might be a suitable method to improve feed efficiency, and to reduce carcass fat deposition in the production of meat-type ducks.

Peptide Domain Involved in the Interaction between Membrane Protein and Nucleocapsid Protein of SARS-associated Coronavirus

Xiao Nan Fang,Lin Bai Ye,Khalid Amine Timani,Shan Shan Li,Ying Chun Zen,Meng Zhao,Hong Zheng,Zheng Hui Wu 한국생활환경학회 2005 BMB Reports Vol.38 No.4

Shotgun analysis on the peritrophic membrane of the silkworm Bombyx mori

( Xiao Wu Zhong ),( Li Ping Zhang ),( Yong Zou ),( Qi Ying Yi ),( Ping Zhao ),( Qing You Xia ),( Zhong Huai Xiang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.11

The insect midgut epithelium is generally lined with a unique chitin and protein structure, the peritrophic membrane (PM), which facilitates food digestion and protects the gut epithelium. We used gel electrophoresis and mass spectrometry to identify the extracted proteins from the silkworm PM to obtain an in-depth understanding of the biological function of the silkworm PM components. A total of 305 proteins, with molecular weights ranging from 8.02 kDa to 788.52 kDa and the isoelectric points ranging from 3.39 to 12.91, were successfully identified. We also found several major classes of PM proteins, i.e. PM chitin-binding protein, invertebrate intestinal mucin, and chitin deacetylase. The protein profile provides a basis for further study of the physiological events in the PM of Bombyx mori.