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토당귀 종자의 형태적 특성과 발아와 입모율에 대한 저장방법,생장조절제 및 프라이밍 처리 효과
안희정,안복주,·김도현,·안영섭,·김영국,박춘근,이상원,·박충범·차선우,·송범헌, 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1
This study was conducted to have basic and applied informations to establish the cultivation method and to improve the cultivation techniques of Angelica gigas Nakai through investigating the rates of germination and seedling establishment with treatment of plant growth regulator and seed priming. The germination rates of round shape seed were about 0.3~12% higher than those of parallel shape. The germination rates with different storage durations were increased at the level of 25oC and 4oC with the storage days, while they were clearly higher at -20oC compared to those at 4oC and 25oC without the storage durations. With the results of germination rates with treating of plant growth regulator and seed priming, their rates with the round shape seeds were comparatively higher about 6% than those with parallel shape seed and they were increased with increasing of the storage durations. The germination rates were generally appeared higher with GA3 than did those with seed priming. The highest germination rate was appeared at 50 ppm GA3 of plant growth regulator and at -0.5 MPa PEG6000 of seed priming. The germination rates?ㅤ ?ㅤ of Angelica gigas were different with different temperature, storage durations and the treatments of plant growth regulator and seed priming. These results could be used according to different cultivating conditions.
Woo-Sik Jo,Doo-Hyun Cho,So-Deuk Park,Young-Bok Yoo,Soon-Ja Seok,Hee-young Jung,Seung-Chun Park 한국버섯학회 2009 한국버섯학회지 Vol.7 No.4
Ganoderma applantaum belonged to Polyporaceae of Basidiomycetes and has been known as one of the popular medicinal mushrooms due to high antitumor and anti-diabetes activity. This study was carried out to obtain the basic information for mycelial culture conditions of Ganoderma applantaum. According to colony diameter and mycelial density, the media for suitable mycelial growth of them were shown in PDA and MEA. The optimum temperature for mycelial growth was 25~30℃The colonies are white, flocculose and underside of the plates is whitish to cream. Carbon and nitrogen sources were mannose and malt extract, respectively. The optimum C/N ratio was 10:1 to 5:1with 2% glucose concentration, vitamin was thiamine- HCl, organic acid was succinic acid, and mineral salt was MgSO4ㆍ7H2O. Present experiments were conducted to determine the possibility of artificial culture with sawdusts of G. applantaum. The pH value which was 6.0 of oak sawdust and6.4 of ricebran. Mycelial density on oak sawdust of G.applantaum spp. was higher than Ganoderma lucidium (a control). Wt. of dried fruiting body (g) of G. applantaum. (origin culture: GBEA-01, strain name: SamJung(三淨)) showed that 16.0 g on oak sawdust bottle cultivation (850㎖), 7.8 g on oak sawdust and G. lucidum. This present study was undertaken to investigate comparative anti-tumor activity of water extracts of G. lucidum ASI 7125 (GLE), G. applantaum SamJung (GAE) in vitro. The anti-tumor activity in the present study was evaluated by sulforhodamine B (SRB) and microtetrazolium (MTT) assay in terms of cell survival level. The results showed that DW, GLE and GAE inhibited proliferation showing both tumor cells.
Research Notes : Detection of Extracellular Enzyme Activities in Ganoderma neo-japonicum
( Woo Sik Jo ),( Ha Na Park ),( Doo Hyun Cho ),( Young Bok Yoo ),( Seung Chun Park ) 한국균학회 2011 Mycobiology Vol.39 No.2
The ability of Ganoderma to produce extracellular enzymes, including β-g1ucosidase, cellulase, avicelase, pectinase, xylanase, protease, amylase, and ligninase was tested in chromogenic media, β-glucosidase showed the highest activity, among the eight tested enzymes. In particular, Ganoderma neo-japonicum showed significantly stronger activity for β-glucosidase than that of the other enzymes. Two Ganoderma lucidum isolates showed moderate activity for avicelase; however, Ganoderma neo-japonicum showed the strongest activity. Moderate ligninase activity was only observed in Ganoderma neo-japonicum. In contrast, pectinase, amylase, protease, and cellulase were not present in Ganoderma. The results show that the degree of activity of the tested enzymes varied depending on the Ganoderma species tested.
Optimal media conditions for the detection of cellulolytic activity in Ganoderma lucidum
Woo-Sik Jo,Soon-Hwa Bae,Doo-Hyun Cho,So-Deuk Park,Young-Bok Yoo,Seung-Chun Park 한국버섯학회 2010 한국버섯학회지 Vol.8 No.2
To determine the optimal media conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35℃. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at 25℃ for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum
Conformation of Group "a" Epitope in Hepatitis B Surface Antigen
Chun, Mun-Ho,Park, Won-Bong,Bok, Jin-Woo,Kim, Ha-Won,Choi, Eung-Chil,Kim, Byong-Kak The Pharmaceutical Society of Korea 1992 Archives of Pharmacal Research Vol.15 No.4
To elucidate structure of group "a" epitope, mouse antibodies that express idiotype monoclonal antibody and anti-idiotype monoclonal antibody against the group specific "a" determinant were purified by hydroxyapatite column. To obtain hepatitis B surface antigens (HBsAg). HBsAg positive blood was sequencially purified by ammonium sulfate precipitation, hydroxyapatite, sepharose 4B column chromatography and ultracentrifugation. The major protein (p25) and glycoprotein (gp30) of HBsAg were isolated by concanavalin-A-sepharose 4B. The ability of p25-gp30 among the HBsAg to inhibit the idiotype-anti-idiotype reaction was dependent on conformation, since reduced and alkylated p25-gp30 virtualy lost their inhibitory capacity when compared to native HBsAg. The data suggest that hepatitis B antigen is a conformational antigen critically dependent upon the disulfide bonds of p25-gp30.
( Woo Sik Jo ),( Ha Na Park ),( Doo Hyun Cho ),( Young Bok Yoo ),( Seung Chun Park ) 한국균학회 2011 Mycobiology Vol.39 No.2
To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the use of four dyes, Congo red, phenol red, remazol brilliant blue, and trypan blue. To observe the effect of pH on the chromogenic reaction, we tested media ranging from 4.5 to 8.0. To research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35℃. On the whole, the best protocol called for Ganoderma neo-japonicum transfer onto media containing Congo red with a pH of 7.0, followed by incubation at 25℃ for 5 days. Our results will be useful to researchers who study extracellular enzyme activity in Ganoderma neo-japonicum.
만삭전 조기양막파열 산모에서 양수과소증이 주산기 예후 및 분만 잠복기에 미치는 영향
구천회 ( Chun Hoe Ku ),박교훈 ( Kyo Hoon Park ),우헌탁 ( Hun Tack Woo ),김문영 ( Moon Young Kim ),김유미 ( You Mi Kim ),이철민 ( Chul Min Lee ),조용균 ( Yong Kyoon Cho ),최훈 ( Hoon Choi ),김복린 ( Bok Lin Kim ),이홍균 ( Hong Kyo 대한주산의학회 2002 Perinatology Vol.13 No.2
연구목적:본 연구의 목적은 만삭전 조기양막파열 산모에서 양수과소증이 주산기 예후 및 분만 잠복기에 미치는 영향을 살펴보고자 한다. 방법:조기양막파열로 입원한 임신 26주에서 35주의 단태임신 98명을 대상으로 후향적 관찰 연구를 시행하였다. 모든 대상 환자에서 입원 직후 경복부 초음파를 시행하여 양수 지수를 측정하였으며 모든 산모와 신생아 의무 기록을 검토하여 양수과소증이 주산기 예후 및 분만 잠복기에 미치는 영향을 조사하였다. 양수과소증은 양수지수가 5 이하일때로 정의하였고 분만 잠복기의 정의는 양막파열부터 분만까지의 시간으로 하였다. 통계 처리는 비율의 비교는 Chi-spuare test 또는 Fisher`s exact test를 사용하였으며, 연속형 변수의 비교는 변수의 특성에 따라 Student-t test 또는 Mann-Whitney U test를 사용하였다. 결과:1) 총 98명의 연구 대상자 중 양수과소증이 존재하지 않은 군이 59명(60%)이었고 양수과소증 군은 39명(40%)이었다. 조기양막파열 산모에서 분만 주수, 분만시 신생아 체중은 양수과소증이 존재한 군에서 양수과소증이 존재하지 않은 군보다 유의하게 낮았으나 1분 아프가 점수 7점 미만의 빈도, 5분 아프가 점수 7점 미만의 빈도, 신생아 이환율 및 주산기 사망률은 두 군 사이에 유의한 차이가 없었다. 2) 조기양막파열 산모에서 양수과소증이 분만 잠복기에 미치는 영향을 알아보기 위하여 산모 및 태아적응증으로 유도분만이나 제왕절개술을 시행한 산모 73명을 제외한 25명을 대상으로 하였다. 분만 잠복기는 양수과소증이 존재하지 않은 군의 중앙값이 41.5시간으로 양수과소증이 존재한 군의 중앙값 44시간과 유의한 차이를 나타내지 않았다. 결론:만삭전 조기양막파열 산모에서 양수과소증은 불량한 주산기 예후 및 분만 잠복기와는 유의한 관련성을 나타내지 않았다. Objectives:To examine the effects of the oligohydramnios on perinatal outcome and latency period in patients with preterm premature rupture of membranes. Methods:We performed a retrospective analysis of 98 singleton pregnancies complicated by preterm premature rupture of membranes, with delivery between 26 and 35 weeks` gestation. Amniotic fluid index was determined using transabdominal ultrasound at admission. All medical records of mothers and neonates were reviewed. Oligohydramnios was defined as amniotic fluid index less or equal to 5.0cm and latency period was defined as time interval from membrane rupture to delivery. Chi-spuare test, Fisher`s exact test, Student-t test, Mann-Whitney U test were used for statistical analysis. Results:1) Of the 98 patients, 59 patients(60%) were oligohydramnios group(AFI≤5.0) and 39 patients(40%) were non-oligohydramnios group(AFI>5.0). Both groups were similar with respect to selected dermographics, gestational age at rupture of the membranes, chorioamnionitis, 1 min Apgar score and 5 min Apgar score. Patients with oligohydramnios demonstrated a lower gestatoinal age at birth and lower birth weight. There were no statistically significant correlations in neonatal morbidity and perinatal mortality between both groups. 2) For comparing latency period, we excluded deliveries of Cesarean section or induction. Comparing the remained group(25 patients), median of latency period in oligohydramnios group were 41.5 hours and median of latency period in non-oligohydramnios group were 44 hours. There were no statistically significant correlations in oligohydramnios and latency period. Conclusion:There were no significant effects of the oligohydramnios on poor perinatal outcome and latency period in patients with preterm premature rupture of membranes