Transcriptional response of Methoprene-tolerant (Met) gene to three insect growth disruptors in Leptinotarsa decemlineata (Say)

Qing-Wei Meng,Qing-Yu Xu,Pan Deng,Kai-Yun Fu,Wen-Chao Guo,Guo-Qing Li 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

Some insect growth disruptors (IGDs), such as pyriproxyfen and halofenozide, may be used to control Leptinotarsa decemlineata. However, their mechanism of action remains elusive. Methoprene-tolerant (Met) mediates juvenile hormone (JH) signal to control numerous essential physiological processes. In the present paper, we identified a Met gene (LdMet). LdMet protein was a typical basic helix-loop-helix/Per-Arnt-Sim (bHLHPAS) transcription factor with a bHLH domain, two PAS domains (PAS-A and PAS-B) and a region called PAS associated C terminal (PAC). Eight conserved amino acids critical for JH binding were located in PAS-B and PAC domains. The temporal expression pattern of LdMet was in accordance with the variation of circulating JH titers. Feeding of juvenoid methoprene or pyriproxyfen, or provide for JH dose-dependently stimulated the expression of LdMet. RNA interference-mediated knockdown of two JH degradation genes increased the transcription of LdMet, while silencing of a JH biosynthesis gene repressed the transcription. Conversely, ingestion of an ecdysteroid agonist halofenozide or 20-hydroxyecdysone (20E) reduced the mRNA levels of LdMet, in a dosedependent manner, whereas knockdown of either ecdysteroidogenesis or 20E signaling genes increased the mRNA accumulation. Providing that the expression of LdMet can be disturbed by methoprene, pyriproxyfen and halofenozide, LdMet may be a potential target of these IGDs in L. decemlineata larvae.

Bond-slip Constitutive Relation between BFRP Bar and Basalt Fiber Recycled-aggregate Concrete

Wen-jing Meng,Hua-xin Liu,Gen-jin Liu,Xiang-qing Kong,Xue-zhi Wang 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.5

In order to investigate the bond stress-slip constitutive relation between BFRP bar and basalt fiber recycled-aggregate concrete, 81 central pullout specimens were tested. Basalt fiber volume percent, fiber length and concrete strength grade were considered to be experimental variables to obtain the bond stress-slip curves. The results showed that adding basalt fiber into recycled-aggregate concrete will reduce the bond stress between BFRP bar and recycled-aggregate concrete but enhance the ductility of bond property; the bond stress increases with the increasing of fiber length and concrete strength grade. The bond stress-slip constitutive model was proposed and the results showed that the model is good in effect of fitting. The constitutive model can reflect the bonding mechanism between BFRP bar and basalt fiber concrete well, it also can provide reference for theoretical analysis and engineering applications of the bond and anchor property between BFRP bars strengthened recycled concrete.

A hemoglobin-based oxygen-carrying biomimetic nanosystem for enhanced chemo-phototherapy and hypoxia alleviation of hepatocellular carcinoma

Jing-Qing Le,Fang Yang,Xun-Huan Song,Ke-Ke Feng,Ling-Wu Tong,Meng-Die Yin,Wen-Zhong Zhang,Ying-Qi Lin,Hui Wu,Jing-Wei Shao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

Tumor microenvironment is characterized by low pH, high reactive oxygen species and hypoxia, whichprovides a suitable environment for cancer growth. The hypoxia not only elevates tumor angiogenesisand metastasis, but also is responsible for the development of treatment resistance, which graduallybecomes a significant impediment for cancer therapy. Therefore, we developed a biomimetic nanosystemcontaining hemoglobin extracted from red blood cells, chemotherapy drug sorafenib, sensitizer ursolicacid and photosensitizer indocyanine green for enhanced chemo-photo combination therapy of hepatocellularcarcinoma, which could not only enhance the chemotherapy effect of sorafenib bowing to thesensitizing effect of ursolic acid, but also achieved synergetic phototherapy in virtue of indocyaninegreen. Besides, the nanoparticles could effectively delivery exogenous oxygen to tumor site and amelioratethe tumor hypoxic environment with the assistance of hemoglobin. The dual-sensitization drugdelivery system was expected to effectively reduce the resistance of traditional treatment methodsagainst tumor hypoxia, providing a novel prospect for the synergistic hepatocellular carcinomatreatment.

Systematic polymorphism analysis of the CYP2C9 gene in Chinese Han and Tibetan populations

Yue-Wen Wang,Xiao-Hong Meng,Wei Yan,Xiao-Qing Zhang,Jin Yang 한국유전학회 2012 Genes & Genomics Vol.34 No.3

Study about polymorphism of the CYP2C9 was not reported in the Chinese Tibetan population and there was no comparison of genetic polymorphism pattern of CYP2C9 between Chinese Han and Tibetan populations. Here we screened the genetic polymorphisms of functional regions of the CYP2C9in 100 unrelated healthy Chinese Han and Tibetan volunteers,respectively, using direct sequencing. A total of 20 variants were detected and there were different distribution of allelic and genotype frequencies, linkage disequilibrium patterns,haplotype structures and htSNPs between the two populations. CYP2C9*3 is a major functional variant of CYP2C9 in the two populations and *11 allele was only detected in Tibetan population. The determined genetic information of CYP2C9in Chinese Han and Tibetan populations might serve as a baseline for larger studies on determining metabolic phenotypes of CYP2C9 substrate drugs and also provide important data for the advance of personalized medicine in Chinese Han and Tibetan populations.

Pd/Ce0.9Cu0.1O1.9-Y2O3 catalysts for catalytic combustion of toluene and ethyl acetate

Xiao-Wen Su,Ling-Yun Jin,Ji-Qing Lu,Meng-Fei Luo 한국공업화학회 2009 Journal of Industrial and Engineering Chemistry Vol.15 No.5

A dual-functional Pd/CuO-CeO2-Y2O3 (Pd/CCY) using CuO-CeO2 as precursor was prepared and tested for catalytic combustion of toluene and ethyl acetate. It was found that the catalyst is much higher active and thermally stable for combustion of both toluene and ethyl acetate, compared with the catalyst prepared with the conventional method. Therefore, the formation of the CuO-CeO2 solid solution before coating is the key to high activity and thermal stability. Moreover, Pd/PdO species are the active phase for oxidation of toluene, while CuO or CuO-CeO2 is responsible for oxidation of ethyl acetate, and the formation of the Pd probably impels the enhancement of activity for the catalyst calcined at 1000℃.

Influence of Particle Shape on the Density and Compressive Performance of Calcareous Sand

Shuai Wang,Xue-Wen Lei,Qing-Shan Meng,Jie-Li Xu,Ling-Feng Xie,Yu-Jie Li 대한토목학회 2020 KSCE Journal of Civil Engineering Vol.24 No.1

Particle shape influence the bearing capacity of calcareous sand foundations in island reef engineering. An improved Computer Image Analysis method that combined with mathematical statistics was proposed to categorize and sort different shapes of calcareous sand particles within the size range of 5 – 10 mm. Three single-shape calcareous sand specimens (granular ﾠ ﾠshape, dendritic shape, long strip shape) were obtained. A new self-developed vibration device was used measuring the maximum density of calcareous, the shape and content as the variables in the orthogonal test. On this basis, the lateral compression tests were carried out to assess the influence of particle shape on the density, compressive performance of calcareous sand. Test results showed that better discriminability was achieved by taking mean flatness (EL/B) as the quantification parameter of the particle shapes. The average flatness EL/B of specimens is approximately normally distributed in the statistical results. The shape and content of sand particles had an obvious influence on the density of calcareous sand. The compressive properties of calcareous sands with different particle shapes have obvious differences. After loaded, the particle size distribution of specimen improved, the average flatness EL/B of the granular-shaped specimens increased, while that of the dendritic-shaped and the long-strip-shaped specimens decreased, the average flatness EL/B of the specimens tended to be uniform. The relative fracture rate Br and the load value Pmax satisfied the exponential function fitting relationship. Therefore, it can be argued that particle shape should be taken into consideration when optimizing the foundation design in island reef engineering to achieve better compressive performance of the foundation.

Numerical Analysis of Solid-Liquid Two-Phase Abrasive Flow in Microcutting Polycrystalline Materials Based on Molecular Dynamics

Jun-Ye Li,Wen-Qing Meng,Kun Dong,Xin-Ming Zhang,Wei-Hong Zhao 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.11

Abrasive flow polishing plays an important role as a type of precision nanomachining technology. In this study, the polishing mechanism of abrasive flow was studied using numerical simulations based on molecular dynamics. In order to study the polishing mechanism of polycrystalline materials, the molecular dynamics simulation of three kinds of polycrystalline materials was carried out. The cutting force, energy, friction coefficient, and surface morphology (during the microcutting process) of the three workpiece materials were analyzed. The analysis shows that the adhesion of atoms in the contact area on the workpiece surface will be affected by liquid phase and the type of abrasive particles, and then the surface quality polished by the abrasive flow will also be influenced. The onset of an abrasive flow polishing process involving plastic deformation (including the nucleation and migration of dislocations, grain boundary shape deformation, and deformation twinning) was observed on the workpiece surface. Defects strongly competed with each other in the form of interactions of dislocations and the grain boundaries, resulting in the migration of twin boundaries. A further analysis of the numerical results for different cutting depths and abrasive properties of the workpiece materials will be useful for understanding the different cutting effects.

The basic helix–loop–helix transcription factors in the Colorado potato beetle Leptinotarsa decemlineata

Kai-Yun Fu,Qing-Wei Meng,Feng-Gong Lüa,Wen-Chao Guo,Tursun Ahmat,Guo-Qing Li 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.2

The basic helix–loop–helix (bHLH) transcription factors possess crucial functions in cell proliferation, determination, differentiation, cell cycle maintenance and homeostasis or stress response pathways. Since a few juvenile hormone analogues such as hydroprene, methoprene and pyriproxyfen targeting a bHLH member Met are already registered for pest management, there is a potential to develop more insecticides targeting various bHLHs. The identification of bHLH members is the first step. Based on the transcriptome and the genome of the Colorado potato beetle Leptinotarsa decemlineata, the most important pest in potato, 49 bHLH members were identified. All LdbHLH memberswere defined by their names and families according to various phylogenetic analyses with bHLH homologues of Drosophila melanogaster, Apis mellifera, Bombyx mori and Tribolium castaneum. Among these LdbHLHs, 17, 10, 10, 1, 10 and 1 members belonged to A, B, C, D, E and F high-order groups, respectively. The results would provide useful background information for future studies on functions of bHLH proteins in the regulation of L. decemlineata development. Moreover, the bHLHs could serve as potential insecticide targets. These proteins could be used in small molecule screen, or in the development of RNAi-based pest management methods.

Biocatalysis and Fermentation Technology : Purification and Characterization of a Thermostable Xylanase from Paenibacillus sp. NF1 and its Application in Xylooligosaccharides Production

( Hong Chen Zheng ),( Ming Zhe Sun ),( Ling Cai Meng ),( Hai Sheng Pei ),( Xiu Qing Zhang ),( Zheng Yan ),( Wen Hui Zeng ),( Jing Sheng Zhang ),( Jin Rong Hu ),( Fu Ping Lu ),( Jun She Sun ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4

High levels of extracellular xylanase activity (211.79 IU/mg) produced by Paenibacillus sp. NF1 were detected when it was submerged-cultured. After three consecutive purification steps using Octyl-Sepharose, Sephadex G75, and Q-Sepharose columns, a thermostable xylanase (XynNF) was purified to homogeneity and showed a molecular mass of 37 kDa according to SDS-PAGE. The specific activity of the purified XynNF was up to 3,081.05 IU/mg with a 14.55-fold purification. The activity of XynNF was stimulated by Ca2+, Ba2+, DTT, and β-mercaptoethanol, but was inhibited by Fe3+, Zn2+, Fe2+, Cu2+, SDS, and EDTA. The purified XynNF displayed a greater affinity for oat spelt xylan with the maximal enzymatic activity at 60°C and pH 6.0. XynNF, which was shown to be cellulose-free, with high stability at high temperature (70°C-80°C) and low pH range (pH 4.0-7.0), is potentially valuable for various industrial applications. The enzyme hydrolyzed oat spelt xylan to yield mainly xylooligosaccharides (95.8%) of 2-4 degree of polymerization (DP2-4). Moreover, the majority of the xylooligosacharides (DP2- 4) products was xylobiose (61.5%). The thermostable xylanase (XynNF) thus seems potentially usefull in the production of xylooligosaccharides.

Combination of Tumor Volume and Epstein-Barr Virus DNA Improved Prognostic Stratification of Stage II Nasopharyngeal Carcinoma in the Intensity Modulated Radiotherapy Era: A Large-Scale Cohort Study

Qiu-Yan Chen,Shao-Yan Guo,Lin-Quan Tang,Tong-Yu Lu,Bo-Lin Chen,Qi-Yu Zhong,Meng-Sha Zou,Qing-Nan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Yang Li,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Chong Zha 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Little is known about combination of the circulating Epstein-Barr viral (EBV) DNA and tumor volume in prognosis of stage II nasopharyngeal carcinoma (NPC) patients in the intensity modulated radiotherapy (IMRT) era. We conducted this cohort study to evaluate the prognostic values of combining these two factors. Materials and Methods By Kaplan-Meier, we compare the differences of survival curves between 385 patients with different EBV DNA or tumor volume levels, or with the combination of two biomarkers mentioned above. Results Gross tumor volume of cervical lymph nodes (GTVnd, p < 0.001) and total tumor volume (GTVtotal, p < 0.001) were both closely related to pretreatment EBV DNA, while gross tumor volume of nasopharynx (GTVnx, p=0.047) was weakly related to EBV DNA. EBV DNA was significantly correlated with progress-free survival (PFS, p=0.005), locoregional-free survival (LRFS, p=0.039), and distant metastasis-free survival (DMFS, p=0.017), while GTVtotal, regardless of GTVnx and GTVnd, had a significant correlation with PFS and LRFS. The p-values of GTVtotal for PFS and LRFS were 0.008 and 0.001, respectively. According to GTVtotal and pretreatment EBV DNA level, patients were divided into a low-risk group (EBV DNA 0 copy/mL, GTVtotal < 30 cm3; EBV DNA 0 copy/mL, GTVtotal  30 cm3; or EBV DNA > 0 copy/mL, GTVtotal < 30 cm3) and a high-risk group (EBV DNA > 0 copy/mL, GTVtotal  30 cm3). When patients in the low-risk group were compared with those in the high-risk group, 3-year PFS (p=0.003), LRFS (p=0.010), and DMFS (p=0.031) rates were statistically significant. Conclusion Pretreatment plasma EBV DNA and tumor volume were both closely correlated with prognosis of stage II NPC patients in the IMRT era. Combination of EBV DNA and tumor volume can refine prognosis and indicate for clinical therapy.