Valveless Piezoelectric Micropump of Parallel Double Chambers

Li Guo,Weiping Yan,Yinghua Xu,Yiru Chen 한국정밀공학회 2012 International Journal of Precision Engineering and Vol. No.

The driving performance of the piezoelectric actuator was simulated by ANSYS software, and the relationship between structure parameters and center displacement/frequency of piezoelectric actuator were obtained. The nozzle/diffuser pipes’structure parameters were optimized according to the results of ANSYS numerical simulation, and flow characteristic parameters such as flow rate and pressure distribution in the pipe were researched. The chamber was manufactured on glass, and nozzle/diffuser pipes were fabricated on <100> silicon by selective-wet etch using MEMS technology. These two components were glued with piezoelectric actuator together to form micropumps with single chamber and micropumps with parallel double chambers. The flow rates of micropumps in different conditions were measured by test system. Finally, a comparison between the test results of micropump with double chambers and that of the single chamber micropump is given.

Effects of face-sheet materials on the flexural behavior of aluminum foam sandwich

Wei Xiao,Chang Yan,Weibo Tian,Weiping Tian,Xuding Song 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.29 No.3

Properties of AFS vary with the changes in the face-sheet materials. Hence, the performance of AFS can be optimized by selecting face-sheet materials. In this work, three types of face-sheet materials representing elastic-perfectly plastic, elastic-plastic strain hardening and purely elastic materials were employed to study their effects on the flexural behavior and failure mechanism of AFS systematically. Result showed face-sheet materials affected the failure mechanism and energy absorption ability of AFS significantly. When the foam cores were sandwiched by aluminum alloy 6061, the AFS failed by facesheet yielding and crack without collapse of the foam core, there was no clear plastic platform in the Load-Displacement curve. When the foam cores were sandwiched by stainless steel 304 and carbon fiber fabric, there were no face-sheet crack and the sandwich structure failed by core shear and collapse, plastic platform appeared. Energy absorption abilities of steel and carbon fiber reinforced AFS were much higher than aluminum alloy reinforced one. Carbon fiber was suggested as the best choice for AFS for its light weight and high performance. The versus strength ratio of face sheet to core was suggested to be a significant value for AFS structure design which may determine the failure mechanism of a certain AFS structure.

Anti‐SOX1 Antibodies in Paraneoplastic Neurological Syndrome

Xuan Sun,Jiping Tan,Hui Sun,Yan Liua,Weiping Guan,Jianjun Jia,Zhenfu Wang 대한신경과학회 2020 Journal of Clinical Neurology Vol.16 No.4

Anti-Sry-like high mobility group box (SOX) 1 antibodies (abs) are partly characterized onconeural autoantibodies (autoabs) due to their correlation with neoplastic diseases. Anti- SOX1 abs are associated with various clinical manifestations, including Lambert-Eaton myasthenic syndrome (LEMS) and paraneoplastic cerebellar degeneration (PCD). However, the clinical characteristics of patients with anti-SOX1 abs have not been described in detail. This review systematically explores the reported patients with anti-SOX1 abs and analyzes these cases for demographic characteristics, clinical features, coexisting neuronal autoabs, neuroimaging findings, treatment, and clinical outcomes. In addition, considering that PCD is the most common paraneoplastic neurological syndrome and that the association between PCD and anti-SOX1 abs remains unclear, we focus on the presence of autoabs in relation to PCD and associated tumors. PCD-associated autoabs include various intracellular autoabs (e.g., anti-Hu, anti-Yo, anti-Ri, and anti-SOX1) and cell-surface autoabs (anti-P/Q-type voltage-gated calcium channel). Commonly involved tumors in PCD are small-cell lung cancer (SCLC), gynecological, and breast tumors. LEMS is the most common clinical symptom in patients with anti- SOX1 abs, followed by PCD, and multiple neuronal autoabs coexist in 47.1% of these patients. SCLC is still the predominant tumor in patients with anti-SOX1 abs, while non-SCLC is uncommon. No consistent imaging feature is found in patients with anti-SOX1 abs, and there is no consensus on either the therapy choice or therapeutic efficacy. In conclusion, the presence of anti-SOX1 abs alone is a potential predictor of an uncommon paraneoplastic neurological disorder, usually occurring in the setting of LEMS, PCD, and SCLC. The detection of anti-SOX1 abs contributes to an early diagnosis of underlying tumors, given the diversity of clinical symptoms and the absence of characteristic neuroimaging features.

Arp2/3 complex regulates adipogenesis by controlling cortical actin remodelling.

Yang, Wulin,Thein, Shermaine,Lim, Chun-Yan,Ericksen, Russell E,Sugii, Shigeki,Xu, Feng,Robinson, Robert C,Kim, Jae Bum,Han, Weiping Portland Press Ltd. 2014 Biochemical journal Vol.464 No.2

<P>Extensive actin cytoskeleton remodelling occurs during adipocyte development. We have previously shown that disruption of stress fibres by the actin-severing protein cofilin is a requisite step in adipogenesis. However, it remains unclear whether actin nucleation and assembly into the cortical structure are essential for adipocyte development. In the present study we investigated the role of cortical actin assembly and of actin nucleation by the actin-related protein 2/3 (Arp2/3) complex in adipogenesis. Cortical actin structure formation started with accumulation of filamentous actin (F-actin) patches near the plasma membrane during adipogenesis. Depletion of Arp2/3 by knockdown of its subunits Arp3 or ARPC3 strongly impaired adipocyte differentiation, although adipogenesis-initiating factors were unaffected. Moreover, the assembly of F-actin-rich structures at the plasma membrane was suppressed and the cortical actin structure poorly developed after adipogenic induction in Arp2/3-deficient cells. Finally, we provide evidence that the cortical actin cytoskeleton is essential for efficient glucose transporter 4 (GLUT4) vesicle exocytosis and insulin signal transduction. These results show that the Arp2/3 complex is an essential regulator of adipocyte development through control of the formation of cortical actin structures, which may facilitate nutrient uptake and signalling events.</P>

Ginsenoside Rg1 alleviates A deposition by inhibiting NADPH oxidase 2 activation in APP/PS1 mice

Han Zhang,Yong Su,Zhenghao Sun,Ming Chen,Yuli Han,Yan Li,Xianan Dong,Shixin Ding,Zhirui Fang,Weiping Li,Weizu Li 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6

Background: Ginsenoside Rg1 (Rg1), an active ingredient in ginseng, may be a potential agent for thetreatment of Alzheimer’s disease (AD). However, the protective effect of Rg1 on neurodegeneration in ADand its mechanism of action are still incompletely understood. Methods: Wild type (WT) and APP/PS1 AD mice, from 6 to 9 months old, were used in the experiment. The open field test (OFT) and Morris water maze (MWM) were used to detect behavioral changes. Neuronal damage was assessed by hematoxylin and eosin (H&E) and Nissl staining. Immunofluorescence,western blotting, and quantitative real-time polymerase chain reaction (q-PCR) were used toexamine postsynaptic density 95 (PSD95) expression, amyloid beta (Ab) deposition, Tau and phosphorylatedTau (p-Tau) expression, reactive oxygen species (ROS) production, and NAPDH oxidase 2(NOX2) expression. Results: Rg1 treatment for 12 weeks significantly ameliorated cognitive impairments and neuronaldamage and decreased the p-Tau level, amyloid precursor protein (APP) expression, and Ab generation inAPP/PS1 mice. Meanwhile, Rg1 treatment significantly decreased the ROS level and NOX2 expression inthe hippocampus and cortex of APP/PS1 mice. Conclusions: Rg1 alleviates cognitive impairments, neuronal damage, and reduce Ab deposition byinhibiting NOX2 activation in APP/PS1 mice.

Conference on "China Politics Research : Theory and Methodology" and other Academic Activities supported in Tianjin, China : Asia Research Center, Nankai University (ARC-NKU)

Yuan Qing,Li Shumei,Liu Ji,Zheng Wei,Huang Weiping,Chen Qianli,Wang Binhui,Yan Erbao,Cao Jiyun The Korea Foundation for Adanced Studies 2010 Asia Research Network Vol.7 No.4

Lipid-Overloaded Enlarged Adipocytes Provoke Insulin Resistance Independent of Inflammation

Kim, Jong In,Huh, Jin Young,Sohn, Jee Hyung,Choe, Sung Sik,Lee, Yun Sok,Lim, Chun Yan,Jo, Ala,Park, Seung Bum,Han, Weiping,Kim, Jae Bum American Society for Microbiology 2015 Molecular and cellular biology Vol.35 No.10

<P>In obesity, adipocyte hypertrophy and proinflammatory responses are closely associated with the development of insulin resistance in adipose tissue. However, it is largely unknown whether adipocyte hypertrophy <I>per se</I> might be sufficient to provoke insulin resistance in obese adipose tissue. Here, we demonstrate that lipid-overloaded hypertrophic adipocytes are insulin resistant independent of adipocyte inflammation. Treatment with saturated or monounsaturated fatty acids resulted in adipocyte hypertrophy, but proinflammatory responses were observed only in adipocytes treated with saturated fatty acids. Regardless of adipocyte inflammation, hypertrophic adipocytes with large and unilocular lipid droplets exhibited impaired insulin-dependent glucose uptake, associated with defects in GLUT4 trafficking to the plasma membrane. Moreover, Toll-like receptor 4 mutant mice (C3H/HeJ) with high-fat-diet-induced obesity were not protected against insulin resistance, although they were resistant to adipose tissue inflammation. Together, our <I>in vitro</I> and <I>in vivo</I> data suggest that adipocyte hypertrophy alone may be crucial in causing insulin resistance in obesity.</P>