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Mingsheng Zhong,Xi Liu,Feng Liu,Yulong Ren,Yunlong Wang,Jianping Zhu,Xuan Teng,Erchao Duan,Fan Wang,Huan Zhang,Mingming Wu,Yuanyuan Hao,Xiaopin Zhu,Ruonan Jing,Xiuping Guo,Ling Jiang,Yihua Wang,Jianmi 한국식물학회 2019 Journal of Plant Biology Vol.62 No.1
Starch is a major storage substance in cerealgrains, and starch biosynthesis is a complex process. In orderto elucidate regulation of the starch biosynthesis pathway, wescreened a series of rice (Oryza sativa L.) endospermmutants. In this study, we identified a floury white-coreendosperm mutant named floury endosperm12 (flo12). Theflo12 mutant exhibited loosely packed starch granules and alower thousand kernel weight compared to wild type. Semithinsections revealed that compound starch grains (SG) inflo12 interior endosperm cells were developed abnormally. Furthermore, amylose content was decreased, while totalprotein content was significantly increased in flo12 grains. Map-based cloning showed that FLO12 encodes rice alanineaminotransferase 1 (OsAlaAT1). OsAlaAT1 is highly expressedin developing endosperm. Subcellular localization showedthat OsAlaAT1 is localized in the cytosol. Moreover, theexpression of most starch synthesis-related genes wasdecreased, while most of the storage protein coding geneshad elevated expression levels in the flo12 mutant. Inaddition, overexpression of the OsAlaAT1 gene increasedgrain weight. In brief, we demonstrated that OsAlaAT1regulates carbon and nitrogen metabolism, which provides anew insight for the improvement of rice quality and yield.
Wang, Wenjun,Ouyang, Kehui,Su, Xifan,Xu, Mingsheng,Shangguan, Xinchen Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.11
The polymorphism of insulin-like growth factor I receptor (IGFIR) gene in 12 pig breeds (total n = 593) was detected by PCR-SacII-restriction fragment length polymorphism and allele A (379 bp) or allele B (235 bp and 144 bp) observed. In the studied breeds, it was found that European pigs principally carried allele A, while Chinese native pig breeds principally carried allele B. In addition, the role of pig IGFIR was investigated in 156 Wanbai pigs and 212 Large Yorkshire pigs. Growth related variables including body weight at birth, 2-, 4- and 6-mo of age and backfat thickness and lean percentage estimated by ultrasonography at 6-mo of age were recorded in analyzing the association between IGFIR gene polymorphism and growth traits. AA-genotype pigs exhibited greater (p<0.05) body weights (BW) at birth, 2- and 6-mo of age, but not at 4-mo of age, than those of the BB-genotype in Wanbai and Yorkshire breeds. Moreover, in the Yorkshire breed, AA-genotype pigs had less backfat thickness (p<0.05) and greater lean percentage (p<0.01) than the BB genotype. Based on these results, it is necessary to do more studies on IGFIR before introducing the IGFIR locus into breeding programs.
Device-to-Device Relay Cooperative Transmission Based on Network Coding
( Jing Wang ),( Mingsheng Ouyang ),( Wei Liang ),( Jun Hou ),( Xiangyang Liu ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.7
Due to the advantages of low transmit power consumption, high spectral efficiency and extended system coverage, Device-to-Device (D2D) communication has drawn explosive attention in wireless communication field. Considering that intra-cell interference caused between cellular signals and D2D signals, in this paper, a network coding-based D2D relay cooperative transmission algorithm is proposed. Under D2D single-hop relay transmission mode, cellular interfering signals can be regarded as useful signals to code with D2D signals at D2D relay node. Using cellular interfering signals and network coded signals, D2D receiver restores the D2D signals to achieve the effect of interference suppression. Theoretical analysis shows that, compared with Amplify-and-forward (AF) mode and Decode-and-forward (DF) mode, the proposed algorithm can dramatically increase the link achievable rate. Furthermore, simulation experiment verifies that by employing the proposed algorithm, the interference signals in D2D communication can be eliminated effectively, and meanwhile the symbol error rate (SER) performance can be improved.
IDO promotes the proliferation and invasion of prostate cancer cells through KYNU
Zhou Hongqing,Wang Wei,Liu Mingsheng,Xie Pingbo,Deng Tibin,Peng Jiaxi,Xu Chenxiang 한국유전학회 2023 Genes & Genomics Vol.45 No.3
Background Prostate cancer (PCa) is one of the most common malignant tumors in male. Objective To explore the effect of indoleamine-2, 3-dioxygenase (IDO) on the proliferation and invasion of PCa cells and the potential mechanism. Methods PCa tissues and normal adjacent tissues were collected from 43 PCa patients. The expression of IDO in PCa tissues and cell lines were detected. The String website was used to search for IDO-related proteins. The GEPIA website was used to analyze the relationship between KYNU and the prognosis of PCa. Cells models of IDO overexpression and/or KYNU silencing were constructed to verify the role of KYNU in regulating PCa. The cell proliferation, apoptosis and invasion ability of PCa cells were detected by CCK-8 assay, Flow cytometry and Transwell assay. Results The IDO levels in PCa tissues and cells were higher than those in normal tissues and cells, which promoted the proliferation and invasion of LNCaP cells, and inhibited apoptosis. Silencing IDO inhibited the cells proliferation and invasion activities, and promoted the cell apoptosis. The high expression of KYNU was related to the poor disease free survival of PCa patients. Inhibiting KYUN significantly inhibited the promotion of PCa induced by IDO. Conclusion IDO is overexpressed in PCa, which promotes the proliferation and invasion of PCa cells, and the cancer-promoting mechanism may be related to KYNU.
Huaixu Cheng,Zhidan Luo,Mingsheng Lu,Song Gao,Shujun Wang 한국미생물학회 2017 The journal of microbiology Vol.55 No.5
The hyperthermophilic α-amylase from Thermococcus sp. HJ21 does not require exogenous calcium ions for thermostability,and is a promising alternative to commercially availableα-amylases to increase the efficiency of industrial processeslike the liquefaction of starch. We analyzed the aminoacid sequence of this α-amylase by sequence alignments andstructural modeling, and found that this α-amylase closelyresembles the α-amylase from Pyrococcus woesei. The geneof this α-amylase was cloned in Escherichia coli and the recombinantα-amylase was overexpressed and purified witha combined renaturation-purification procedure. We confirmedthermostability and exogenous calcium ion independencyof the recombinant α-amylase and further investigatedthe mechanism of the independency using biochemical approaches. The results suggested that the α-amylase has a highcalcium ion binding affinity that traps a calcium ion that wouldnot dissociate at high temperatures, providing a direct explanationas to why the addition of calcium ions is not requiredfor thermostability. Understanding of the mechanism offersa strong base on which to further engineer properties of thisα-amylase for better potential applications in industrial processes.
A Study on the Success Factors of Sinar Mas Group in Indonesia
Ruixuan Chen,Yuxue Wang,최명철,Zuoxin He,Mingsheng Li 국제문화기술진흥원 2023 International Journal of Advanced Culture Technolo Vol.11 No.2
With the acceleration of globalization and economic integration, multinational enterprises are becoming more and more important in the international market, and Sinar Mas Group, one of the largest integrated companies in Indonesia, has become one of the most influential overseas Chinese companies in Indonesia due to its outstanding performance and leadership position. This study aims to analyze the success factors of Sinar Mas Group and reveal its competitive advantages in the global market. Founded in 1962, Sinar Mas Group is an integrated multinational company headquartered in Indonesia, with significant presence in various fields such as paper, palm oil, real estate, finance and energy, with operations in Indonesia and many other countries and regions around the world. This study investigates the aspects of corporate history, corporate status, and corporate competitiveness. In addition, this study analyzes the reasons for Sinar Mas Group's success using the SWOT model. By comprehensively analyzing these key factors, this paper provides readers with an insight into the vitality and remarkable achievements of this Indonesian corporate giant in the global market. This study will help Korea enter Indonesia and contribute to Indonesian business research.
Sifang Zhao,Qiang Song,Mingsheng Wang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.12
Rolling element bearings are widely employed in rotating machines. The health monitoring and the residual life prediction of bearings are significant to the reliable operation of the equipment. This paper deals with the principle of a time-domain-based residual life prediction method for rolling element bearings. In particular, a quantitative evaluation of bearing degradation is performed by evaluating the sensitivity of the 13 time-domain features. Meanwhile, the optimal feature set is generated through a sensitivity evaluation analysis approach. Next, the max-min normalization and the multiple linear regression (MLR) methods are applied to construct a healthy index. Based on the healthy index, the degradation trajectory is obtained by using the locally weighted scatterplot smoothing (LOWESS) method. Two data sets generated by the outer race fault are used to verify the effectiveness of the proposed method. The experimental results show that the proposed method can realize the residual life prediction of rolling element bearings effectively.
Song Lu,Shi Yuxia,Zhang Hongfei,Wang Zhengbing,Liu Xiaomeng,Yang Mingsheng 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.2
The mitochondrial genome (mitogenome) has been extensively used in phylogenetics and species-level evolu tionary investigations. The lepidopteran family Tortricidae (leaf-roller moths), including the genus Grapholita, contains numerous species of economic importance, but for the majority of Grapholita species, their mitogenomes remain poorly studied. Here, we sequence and annotate the full mitogenome of Grapholita delineana, an important pest of hemp worldwide and compare it with the mitogenomes of two congeneric species available from GenBank. The G. delineana mitogenome is 15,599 bp long, including 37 typical mitochondrial genes and an A + T-rich region. Gene content, order and orientation are identical to other reported tortricid mitogenomes. Analyses of nucleotide diversity, Ka/Ks, genetic distance and number of variable sites together suggest that nad6 is the fastest-evolving gene among the mitochondrial PCGs of Grapholita. Our analyses indicate that Grapholita, as presently defined, is not monophyletic, confirming previous morphological and multiple-gene studies, using mitogenomic evidence. Our study provides information on comparative mitogenomics of Tortricidae especially Grapholita.