구강암 절제후 대흉근 근피판을 이용한 악안면 재건술의 임상적 고찰

김명진(Myung jin Kim),정필훈(Pil Hoon Choung),김종원(Jong Won Kim) 대한구강악안면외과학회 1990 대한구강악안면외과학회지 Vol.16 No.1

The authors studied 22 patients in whom a pectoralis major myocutaneous flap was used for reconstruction of maxillofacial defect after surgical ablation of oral cancer, and the results obtained were as follows; 1. The most oral cancer was squamous cell carcinoma(81.8%) and the major type of TNM classifiation was T₂NoMo(31.8%). 2. Most of all reconstruction sites were intraoral defects(81.8%) as following orders; gingiva(31.8%), buccal mucosa(18.2%), tongue(18.2%), floor of mouth (13.6%), soft palate(9.1%), retromolar pad(9.1%). The mean size of the skin flap was 7.2×9.0㎝. 3. The pectoralis major myocutaneous flap covered carotid artery in 17 RND cases(77.3%), and metal plate which was temporary substitute of the resected mandible(27.3%), and there was no complication. But the pectoralis major osteomyocutaneous flap with rib seemed to be lack of reliability for reconstruction of the madible. 4. The pectoralis major myocutaneous flap can be used combined with other flap such as sternocleidomastoid(13.6%), cervicopectoral(9.1%), cervicofacial(4.5%), trapezius myocutaneous flap(4.5%), and skin grafts(18.2%). 5. The complications were orocutaneous fistula(9.1%), dehiscence(9.1%), infection(4.5%), partial necrosis(4.5%), and total necrosis(4.5%) in 6 cases(27.3%). 6. It would be appear that the pectoralis major myocutaneous flap is a reliable method and its complications can be reduced by tension-free design and good blood supply to the flap.

인회석 박막 피복 도관과 Brain-derived neurotrophic factor(BDNF) 유전자 이입 슈반세포를 이용한 백서 좌골신경 재생에 관한 연구

최원재(Won-Jae Choi),안강민(Kang-Min Ahn),황순정(Soon-Jeong Hwang),정필훈(Pill-Hoon Choung),김명진(Myung-Jin Kim),김남열(Nam-Yeol Kim),유상배(Sang-Bae Yoo),장정원(Jeong-Won Jahng),김현만(Hyun-Man Kim),김중수(Joong-Soo Kim),김윤희(Yun 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.3

Purpose of Study: Peripheral nerve regeneration depends on neurotrophism of distal nerve stump, recovery potential of neuron, supporting cell like Schwann cell and neurotrophic factors such as BDNF. Peripheral nerve regeneration can be enhanced by the conduit which connects the both sides of transected nerve. The conduit maintains the effects of neurotrophism and BDNF produced by Schwann cells which can be made by gene therapy. In this study, we tried to enhance the peripheral nerve regeneration by using calcium phosphate coated porous conduit and BDNF-Adenovirus infected Schwann cells in sciatic nerve of rats. Materials and Methods: Microporous filter which permits the tissue fluid essential for nerve regeneration and does not permit infiltration of fibroblasts, was made into 2mm diameter and 17mm length conduit. Then it was coated with calcium phosphate to improve the Schwann cell adhesion and survival. The coated filter was evaluated by SEM examination and MTT assay. For effective allogenic Schwann cell culture, dorsal root ganglia of 1-day old rat were extracted and treated with enzyme and antimitotic Ara-C. Human BDNF cDNA was obtained from cDNA library and amplified using PCR. BDNF gene was inserted into adenovirus shuttle vector pAACCMVpARS in which E1 was deleted. We infected the BDNF-Ad into 293 human mammary kidney cell-line and obtained the virus plaque 2 days later. RT-PCR was performed to evaluate the secretion of BDNF in infected Schwann cells. To determine the most optimal m.o.i of BDNF-Ad, we infected the Schwann cells with LacZ adenovirus in 1, 20, 50, 75, 100, 250 m.o.i for 2 hours and stained with 􀝜-galactosidase. Rats(n=24) weighing around 300g were used. Total 14mm sciatic nerve defect was made and connected with calcium phosphate coated conduits. Schwann cells(1x106) or BDNF-Ad infected Schwann cells(1x106) were injected in conduit and only media(MEM) was injected in control group. Twelve weeks after surgery, degree of nerve regeneration was evaluated with gait analysis, electrophysiologic measurements and histomorphometric analysis. Results: 1. Microporous Millipore filter was effective conduit which permitted the adhesion of Schwann cells and inhibited the adhesion of fibroblast. We could enhance the Schwann cell adhesion and survival by coating Millipore filter with calcium phosphate. 2. Schwann cell culture technique using repeated treatment of Ara-C and GDNF was established. The mean number of Schwann cells obtained 1 and 2 weeks after the culture were 1.54±4.0×106 and 9.66±9.6×106. 3. The mRNA of BDNF in BDNF-Ad infected Schwann cells was detected using RT-PCR. In Schwann cell 0.69 μg/μl of DNA was detected and in BDNF-Adenovirus transfected Schwann cell 0.795 μg/μl of DNA was detected. The most effective infection concentration was determined by LacZ Adenovirus and 75 m.o.i was found the most optimal. Conclusion: BDNF-Ad transfected Schwann cells successfully regenerated the 14mm nerve gap which was connected with calcium phosphate coated Millipore filter. The BDNF-Ad group showed better results compared with Schwann cells only group and control group in aspect to sciatic function index, electrophysiologic measurements and histomorphometric analysis.

Fission Mo-99 제조를 위한 공정개발 연구

정원명 ( Won Myung Choung ),박진호 ( Jin Ho Park ),황두성 ( Doo Seong Hwang ),김연구 ( Yeon Ku Kim ),이규일 ( Kue Il Lee ),박경배 ( Kyung Bae Park ) 한국공업화학회 2001 응용화학 Vol.5 No.1

임프란트 식립을 위한 하치조신경 전위술 후 신경손상 및 회복에 대한 임상연구

김명진(Myung Jin Kim),김태영(Tae Young Kim),황경균(Kyeng Gyun Hwang),정필훈(Pill Hoon Choung),김수경(Soo Kyung Kim),김종원(Jong Won Kim),김규식(Kyoo Sik Kim) 대한구강악안면외과학회 1996 대한구강악안면외과학회지 Vol.22 No.1

In case of severe alveolar bony resorption in the edentulous mandible, implant placement posterior to the mental foramen is a problematic procedure. Inferior alveolar nerve transpositioning is often required for implant installation on premolar and molar area for the purpose of more functional prosthodontic rehabilitation. Installation of longer implant fixtures can be performed by this method avoiding the damage of inferior alveolar nerve. We checked Orthopantomograph or MPR(multiplanar reformatted) CT in order to evaluate the anatomical relationship of residual ridge and inferior alveolar nerve accurately and experienced mild to moderate nerve damages on 11 sites, in 7 patients who were received transpositioning of inferior alveolar nerve from 1992 to january, 1995, and had performed serial follow-up study about the recovery of neurosensory function periodically. We can extended the application of implant surgery and get several results as follows about sensory nerve recovery. 1. In all of the cases, the decrease of sensory function were observed postoperatively. 2. Tinel s sign appeared in 8 cases at 1-2 months postoperatively and continued for 2-3 months and disappeared at 6 months postoperatively, and the type of sensory dysfunction was changed as anesthesia, dysesthesia, paresthesia in order and to the normal sensation in 63.6% at 1 year postoperatively. 3. The pain sensation in skin surface was recovered in 68.2% at 6 months, in 81.8% at 12 months postoperatively and the tactile sensation was recovered in 40.1% at 6 months in 77.3% at 12 months postoperatively. 4. Two-point discriminations were checked as normal range in 54.5% of the operation sites at 6 months, 81.8% at 12 months postoperatively, and the area of sensory dysfunction on skin surface diminished by degrees with time. 5. The nerve damage appeared in all the cases postoperatively and the sensory recovery times were different depend on to the amount of nerve damage during the operation. The degree of the recovery of sensory function increased with time, but the permanent slight depression of sensory function remained in a few cases. Therefore patients should be informed enough about that preoperatively.

기하학적 변위 이론을 적용한 VLCC 최종종강도 평가

정준모(Joonmo Choung),남지명(Ji-Myung Nam),Gokhan Tansel Tayyar,윤성원(Sung-Won Yoon),이강수(Kangsu Lee) 대한조선학회 2013 大韓造船學會 論文集 Vol.50 No.4

Radiation Dose Assessment of ACP Hotcell for Spent Fuel Treatment in Normal Operation & Accident Case

Dong-Hak Kook,Won-Myung Choung,Jeong-Hoe Ku,Il-Je Cho,Eun-Pyo Lee,Gil-Sung You 한국방사성폐기물학회 2004 방사성폐기물학회지 Vol.2 No.3

사용후핵연료의 효율적인 관리를 위하여 원자력연구소에서 개발중인 사용후핵연료 차세대관리 종합공정(ACP)은 공정타당성연구 단계를 마치고 이의 실증을 위한 - type핫셀 건설 단계에 이르렀다. 핫셀의 설계에 앞서 사용후핵 연료를 취급하게 되는 과정에서 발생할 수 있는 방사능에 대한 환경영향평가를 정상운전 시와 사고발생 시로 나누어 수행하였다. 평가에 필요한 자료들은 공정의 개념설계 보고서와 최근 연구소부지 기상 테이터 및 부지특성 자료를 바탕으로 하였으며 기존의 유사한 시설에 대한 평가방법을 참조하였다. 각 핵종별 발생량과 방출량을 계산하여 피폭선량을 계산하였으며 평가결과 원자력법관련 규제기준과 핫셀이 위치하게 되는 IMEF 건물의 안전성분석 기준보다 매우 안전한 결과를 얻어 시설 운영에 대한 안전성을 확보하였다. Advanced spent fuel Conditioning Process(ACP) project which is under development for efficient spent fuel management has finished process feasibility study and is preparing - type hot cell construction for process experimentation. Radiation dose evaluation for the radioactive nuclides were preliminarily performed for normal operation and accident case with the basic concept design report, the meteorological data and the recent site specific data. According to the production and release rate of nuclides, dose evaluations for residents around facility were performed. The evaluation result shows a safe margin for regulation limits and SAR(Safety Analysis Report) limit of IMEF(Irradiated Material Examination Facility) where this facility will be constructed.

DESIGN AND CONSTRUCTION OF AN ADVANCED SPENT FUEL CONDITIONING PROCESS FACILITY (ACPF)

GIL-SUNG YOU,WON-MYUNG CHOUNG,JEONG-HOE KU,IL-JE CHO,DONG-HAK KOOK,KIE-CHAN KWON,EUN-PYO LEE,WON-KYUNG LEE 한국원자력학회 2009 Nuclear Engineering and Technology Vol.41 No.6

KAERI has worked on the development of an advanced spent fuel conditioning process (ACP) since 1997. A hot cell facility, termed the ACPF, has also been developed. The ACPF consists of two air-sealed hot cells. The results of a safety analysis as part of the license procurement process stipulated by the Korean Government showed that the facility was designed safely. After its construction, an integrated performance test was performed. The results of this test confirmed that the facility satisfies the design requirements.

A Status of Technology and Policy of Nuclear Spent Fuel Treatment in Advanced Nuclear Program Countries and Relevant Research Works in Korea

Gil-Sung You,Won-Myung Choung,Jeong-Hoe Ku,Il-Je Cho,Dong-Hak Kook,Kie-Chan Kwon,Won-Kyung Lee,Eun-Pyo Lee,Dong-Hee Hong,Ji-Sup Yoon,Seong-Won Park 한국방사성폐기물학회 2007 방사성폐기물학회지 Vol.5 No.4

전세계 주요 원자력선진국들의 사용후핵연료 처리에 대한 기술 및 정책현황을 알아보고 향후 우리나라의 연구방향을 제시해 보았다. 재처리 정책을 가진 소위 핵연료주기 국가들은 최근 선진핵 연료주기기술에 기초한 새로운 사용후핵 연료 관리정책을 발표하였다. 그 정책은 사용후핵연료 내에 함유된 우라늄 또는 초우란 원소들을 재순환하고 고독성의 방사성 물질 및 장반감기를 가진 물질들을 소멸하거나 단반감기 원소로 변환하는데 초점을 맞추고 있다. 이러한 정책은 원자력의 자원 활용성을 높일 뿐만 아니라, 영구 처분할 고준위폐기물의 양을 감소시켜 궁극적으로 원자력의 지속가능성을 높여 준다. PUREX 방법에 기초한 습식재처리를 우선순위로 선택한 대부분의 국가들은 이 습식방법이 건식방법에 비해 실용화에 앞서 있음을 그 선택 의 근거로 든다. 그러나 습식방법은 건식에 비해 핵확산저항성 측면에서 더욱 민감하다. 왜냐하면 이 습식방법은 약간의 공정수정에 의해 순수 플루토늄을 회수 할 수 있기 때문이다. 반면에 아직까지 실용화 단계까지는 도달해 있지 않지만 고온 용융염을 사용하는 Pyroprocess와 같은 건식처리 방법은 순수한 플루토늄을 회수 할 수 없어서 핵비확산성 측면에서 유리하며, 제4세대 원자로로 고려되는 고속로의 핵연료주기 등에도 여러 가지 이점을 가지고 있다. 따라서 우리나라의 경우 현재 이 Pyroprocess에 대한 연구가 활발히 진행되고 있다. Status on the spent nuclear fuel management policy and R&D plan of the major countries is surveyed. Also the prospect of the future R&D plan is suggested. Recently so-called fuel cycle nations, which have the reprocess policy of the spent fuel, announced new spent fuel management policy based on the advanced fuel cycle technology. The policy is focused to transmute highly radioactive material and material having a very long half-life, and to recycle the Pu and U contained in the spent fuel. In this way the radio-foxily of the spent fuel as well as the amount of the high level waste to be eventually disposed can greatly be reduced. Most of countries selected the wet process as a primary option for the treatment of the spent fuel since the advanced wet process, which is based on the existing PUREX process, looks more feasible as compared with the dry process. The wet process, however, is much more sensitive in terms of proliferation-resistance compared with the dry process. The pure Pu can easily be obtained by simply modifying the process. On the other hand the pure Pu can not be extracted in the dry process based on the high temperature molten salt process such as a pyroprocess. Even though the pyroprocess technology is very premature, it has a great merit. Thus it is necessary for Korea to have a long term strategy for pursuing a spent fuel treatment technology with a proliferation resistance and a great merit for the GEN-IV fuel cycles. Pyroprocess is one of the best candidates to satisfy these purposes.

Directional Matrix Nanotopography with Varied Sizes for Engineering Wound Healing

Kim, Jangho,Bae, Won-Gyu,Kim, Yeon Ju,Seonwoo, Hoon,Choung, Han-Wool,Jang, Kyoung-Je,Park, Sunho,Kim, Bog Hee,Kim, Hong-Nam,Choi, Kyoung Soon,Kim, Myung-Sun,Choung, Pill-Hoon,Choung, Yun-Hoon,Chung, J Wiley (John WileySons) 2017 Advanced healthcare materials Vol.6 No.19

DESIGN AND CONSTRUCTION OF AN ADVANCED SPENT FUEL CONDITIONING PROCESS FACILITY (ACPF)

You, Gil-Sung,Choung, Won-Myung,Ku, Jeong-Hoe,Cho, Il-Je,Kook, Dong-Hak,Kwon, Kie-Chan,Lee, Eun-Pyo,Lee, Won-Kyung Korean Nuclear Society 2009 Nuclear Engineering and Technology Vol.41 No.6

KAERI has worked on the development of an advanced spent fuel conditioning process (ACP) since 1997. A hot cell facility, termed the ACPF, has also been developed. The ACPF consists of two air-sealed hot cells. The results of a safety analysis as part of the license procurement process stipulated by the Korean Government showed that the facility was designed safely. After its construction, an integrated performance test was performed. The results of this test confirmed that the facility satisfies the design requirements.