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Sensitive-stage embryo irradiation affects embryonic neuroblasts and adult motor function
WAGLE RAM,Song Young-Han 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.2
Background Cranial radiation therapy for treating childhood malignancies in the central nervous system or accidental radiation exposure may result in neurological side effects in surviving adults. As tissue homeostasis is maintained by stem cells, understanding the effect of radiation on neural stem cells will provide clues for managing the neurological effects. Drosophila embryos were used as a model system whose sensitivity to irradiation-induced cell death changes from the sensitive to resistant stage during development. Objective Drosophila embryos at the radiation-sensitive stage were irradiated at various doses and the radiation sensitivity was tested regarding the appearance of apoptotic cells in the embryos and the embryonic lethality. Cell fates of the neural stem cells called neuroblasts (NBs) and adult motor function after irradiation were also investigated. Result Irradiation of Drosophila embryos at the radiation-sensitive stage resulted in a dose-dependent increase in the number of embryos containing apoptotic cells 75 min after treatment starting at 3 Gy. Embryonic lethality assayed by hatch rate was induced by 1 Gy irradiation, which did not induce cell death. Notably, no apoptosis was detected in NBs up to 2 h after irradiation at doses as high as 40 Gy. At 3 h after irradiation, as low as 3 Gy, the number of NBs marked by Dpn and Klu was decreased by an unidentifi ed mechanism regardless of the cell death status of the embryo. Furthermore, embryonic irradiation at 3 Gy, but not 1 Gy, resulted in locomotor defects in surviving adults. Conclusion Embryonic NBs survived irradiation at doses as high as 40 Gy, while cells in other parts of the embryos underwent apoptosis at doses higher than 3 Gy within 2 h after treatment. Three hours after exposure to a minimum dose of 3 Gy, the number of NBs marked by Dpn and Klu decreased, and the surviving adults exhibited defects in locomotor ability.
Aditi Wagle,성수희,Bing Tian Zhao,우미희,정현아,최재수 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.4
Hizikia fusiformis (Harvey) Okamura is a brownseaweed widely used in Korea and Japan, and it containsdifferent therapeutically active constituents. In the presentstudy, we investigated the activities of glycyrrhizin isolatedfrom H. fusiformis, including its metabolites, 18a- and18b-glycyrrhetinic acid against Alzheimer’s disease (AD)via acetyl and butyrylcholinesterase and b-site amyloidprecursor protein cleaving enzyme 1 (BACE1) inhibition. Among these three compounds, 18b-glycyrrhetinic acid(IC50 = 8.93 ± 0.69 lM) demonstrated two fold potentactivity against BACE1 compared to the positive control,quercetin (IC50 = 20.18 ± 0.79 lM). Additionally, glycyrrhizinwith an IC50 value of 20.12 ± 1.87 lM showedsimilarity to quercetin, while 18a-glycyrrhetinic acidshowed moderate activity (IC50 = 104.35 ± 2.84 lM). Akinetic study revealed that glycyrrhizin and 18b-glycyrrhetinicacid were non-competitive and competitiveinhibitiors of BACE1, demonstrated via Ki values of 16.92and 10.91 lM, respectively. Molecular docking simulationstudies evidently revealed strong binding energy of thesecompounds for BACE1, indicating their high affinity andcapacity for tighter binding to the active site of the enzyme. These data suggest that glycyrrhizin isolated from theedible seaweed, H. fusiformis and its metabolite, 18b-glycyrrhetinicacid demonstrated selective inhibitory activityagainst BACE1 to alleviate AD.
Bishnu Hari Wagle,Ram P. Sharma 한국산림과학회 2012 Forest Science And Technology Vol.8 No.1
Individual tree growth models are important decision-making tools for forest management. We developed individual tree basal area growth models with Blue pine (Pinus wallichiana) data from Lete and Kunjo areas of Mustang district in Nepal. The sample trees were identified from all applicable ages, sizes, site qualities, and stand conditions and were cut. Diameters and ages were measured on the cut surface of stump (at 30 cm above ground). With the application of the auto-regressive error-structured modelling approach, we fitted Bertalanffy function to the data from 94 stumps by using basal area growth per year as dependent variable and stump age or stump diameter as independent variable. The age-independent individual tree basal area growth model showed better fits (R^2_adj=0.8324) than its agedependent counterpart (R^2_adj=0.8174). Because of having better fits and being easier for application, the ageindependent model is recommended for predicting basal area growth per year at an individual tree level for Blue pine across Lete and Kunjo areas of Mustang district.
A study on the methods for making iron oxide aerogel
유정근,공혁진,Roshan Wagle,손병현,김인기,김태호 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.72 No.-
Iron oxide (Fe2O3) aerogel powders were prepared by solvent exchange method using n-butanol withouta supercriticalfluid drying. The obtained aerogel powders have a porosity of 94%, a BET specific surfacearea of 421.1 m2/g, and an average pore size of 7.9 nm. The aerogel powders possessed a BET specificsurface area of 51.8 m2/g even after calcination at 500 C for 2 h despite the effect of pore shrinkage andparticle coagulation. Initially, the Fe2O3 aerogel was amorphous, but some parts of it were changed to acrystalline structure after heat treatment.