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Heat Stress Induced Potato virus X-mediated CRISPR/Cas9 Genome Editing in Nicotiana benthamiana
Jelli venkatesh,이서영,강화정,이세영,이정호,강병철 한국육종학회 2022 Plant Breeding and Biotechnology Vol.10 No.3
Targeted genome editing using CRISPR/Cas nucleases has become the standard approach for creating mutant plants. Significant progress has been made to enhance the editing efficiencies through optimizing CRISPR/Cas expression, including applyingheat stress. In this study, we used heat stress to enhance the Potato virus X (PVX)-mediated CRISPR/Cas9 mutagenesis in Nicotianabenthamiana. We show that heat stress at 4-5 days after PVX inoculation effectively increases the mutagenesis efficiency of Cas9nuclease. We observed up to a 5-8% increase in mutation efficiency depending on the sgRNA construct when heat stress is applied tothe pPVX-Cas9::sgRNA infiltrated samples. Furthermore, analysis of the effect of the heat stress on the pattern of mutation types in thetarget gene regions showed no obvious changes in CRISPR/Cas9 induced mutagenesis pattern between heat stress treated and no heatstress treated samples. Overall, our experiments demonstrate that heat stress treatment at the optimal time after viral inoculation is mosteffective in increasing the PVX-mediated CRISPR/Cas9 editing efficiency in plants.
Development of Bi gene‑based SNP markers for genotyping for bitter‑free cucumber lines
Jelli venkatesh,송기환,이정호,권진경,강병철 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.2
High contents of cucurbitacin compounds result in a bitter taste in cucumbers. The Bi locus mapped to chromosome 6 is responsible for cucumber bitterness. The Bi gene encodes oxidosqualene cyclase, a key enzyme in the cucurbitacin biosynthetic pathway. Two alleles of the Bi gene responsible for the phenotypic variation in bitterness differ by a single point mutation in the coding region. An efficient genotyping system is necessary to allow breeders to screen for bitterness alleles in their breeding programs. In the present study, the single nucleotide polymorphism located within the Bi gene, which is directly linked to cucumber bitterness, was utilized for gene-based marker development. We developed reliable and costeffective high-resolution melting (HRM)- and Kompetitive Allele-Specific PCR (KASP)-based molecular markers (BiHRM1 and Bi-KASP). These gene-based markers should enhance the accuracy and effectiveness of marker-assisted selection for bitter-free cucumber lines in cucumber breeding programs.
Venkatesh, Jelli,Kang, Byoung-Cheorl Elsevier 2019 Current opinion in plant biology Vol.50 No.-
<P>Elevated ambient temperatures will likely be a key consequence of climate change over the next few decades. Adverse climatic changes could make crop plants more vulnerable to a number of biotic and abiotic stresses, which would have a major impact on worldwide food production in the future. Recent studies have indicated that elevated temperatures directly and/or indirectly affect plant–pathogen interactions. Elevated temperatures alter multiple signal transduction pathways related to stress responses in the host plant. High temperatures can also influence plant pathogenesis, but little is known about the molecular mechanisms associated with such effects. An improved understanding of the molecular genetic mechanisms involved in plant immune responses under elevated temperatures will be essential to mitigate the adverse effects of climate change to ensure future food security. In this review, we discuss recent advances in our understanding of the effects of temperature on resistance (<I>R</I>) gene and/or regulators of <I>R</I> genes in plant defense responses and summarize current evidence for tradeoffs between plant growth and immunity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>R</I> genes are critical components of temperature-modulated defense mechanisms. </LI> <LI> PhyB and PIF4 integrate temperature and defense responses. </LI> <LI> Plant immune responses are constrained by tradeoffs with temperature-dependent growth. </LI> <LI> The temperature sensitivity of regulators of <I>R</I> genes plays an important role in modulation of plant defense. </LI> </UL> </P>
Venkatesh, Jelli,An, Jeongtak,Kang, Won-Hee,Jahn, Molly,Kang, Byoung-Cheorl American Phytopathological Society] 2018 Phytopathology Vol.108 No.1
<P> Pepper mottle virus (PepMoV) is the most common potyvirus infection of pepper plants and causes significant yield losses. The Pvr7 gene from Capsicum chinense PI159236 and the Pvr4 gene from C. annuum CM334 both have been reported to confer dominant resistance to PepMoV. The Pvr7 locus conferring resistance to PepMoV in C. annuum '9093' was previously mapped to chromosome 10. To develop a high-resolution map of the Pvr7 locus in 9093, we constructed an intraspecific F<SUB>2</SUB> mapping population consisting of 916 individuals by crossing PepMoV-resistant C. annuum '9093' and the PepMoV-susceptible C. annuum 'Jeju'. To delimit the Pvr7 target region, single-nucleotide polymorphism (SNP) markers derived from the Pvr4 region were used for genotyping the F<SUB>2</SUB> population. Molecular mapping delimited the Pvr7 locus to a physical interval of 258 kb, which was the same region as Pvr4 on chromosome 10. Three SNP markers derived from Pvr4 mapping perfectly cosegregated with PepMoV resistance. Sequencing analyses of the Pvr7 flanking markers and the Pvr4-specific gene indicated that Pvr7 and Pvr4 are the same gene. Resistance spectrum analysis of 9093 against pepper potyviruses showed that 9093 has a resistance spectrum similar to that of cultivar CM334. These combined results demonstrate that, unlike previously thought, the dominant PepMoV resistance in 9093 could be derived from C. annuum 'CM334', and that Pvr4 and Pvr7 should be considered as the same locus. </P>
Jelli Venkatesh,Chandrama Prakash Upadhyaya,유재웅,Ajappala Hemavathi,김두환,Reto J. Strasser,박세원 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.4
L-Ascorbate plays a vital role in the alleviation of salinity stress in crop plants. Overexpression of the ascorbate pathway enzyme D-galacturonic acid reductase in transgenic potato plants confers improved tolerance to various abiotic stresses. These transgenic potato plants were further studied for their primary photosynthetic performances under salinity stress. The changes in primary photochemistry of PSII induced by salinity stress were studied using JIP-test. Analysis of the fast phase chlorophyll a fluorescence transients indicated that there was a differential effect of salinity stress on different sites of the photosynthetic machinery. The transgenic potato leaves exhibited a gain in the ability for restraining the energy loss when they were imposed by salinity stress. These observations suggest that under salinity stress, the photosynthetic energy conservation in the transgenic plants was more effective than in the wild-type plants.
[PD-0020] High Efficient Transgene-free Genome Editing in Solanum lycopersicum using Potato Virus X
Seo-Young Lee(Seo-Young Lee),Jelli Venkatesh(Jelli Venkatesh ),Joung-Ho Lee(Joung-Ho Lee),Seungki Back(Seungki Back),Jung-Min Kim(Jung-Min Kim),Byoung-Cheorl Kang(Byoung-Cheorl Kang) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-