일부 폐결핵환자의 HBs Ag. 발현빈도와 HBs Ag. 음양성자 간의 GOT 치와 GPT 치의 비교 검토

김태전,김승곤 서울保健大學 1981 論文集 Vol.1 No.1

폐결핵환자 300명을 대상으로 HBs ag.와 GOT와 GPT치를 검사한 성적을 요약하면 다음과 같다. 1) 폐결핵환자에서의 HBs ag. 양성율은 20%로서 대조군의 4.1%보다 유의하게 높았다(p<0.05). 2)HBs ag. 양성자의 GOT(43.2±2.0unit), GPT(29.3±1.8unit)치는 음성자의 GOT(35.4±1.3unit), GPT(19.3±1.9unit)치보다 다소 높은 경향을 보였다. 따라서 폐결핵환자 치료시에는 완전 멸균된 주사침을 사용해야 함은 물론 항결핵제가 간에 미치는 부작용을 감안 HBs ag.에 대한 검사를 반드시 실시해야 할 것이며 HBs ag.의 음양성이 GOT치나 GPT치에 큰 영양을 주지는 못하는 것으로 생각된다. This study was done to see HBs antigen positive rate of patients, with pulmonary tuberculosis, and to compare GOT, GPT levels between HBs antigen positive and negative patients. The results obtained were summarized as following: 1) As HBs antigen positive rate was 20% in patients with pulmonary tuberculosis, it was significantly higher than HBs positive rate (4.1%) of control group. (P<0.05). 2) GOT, GPT levels of HBs antigen positive patients (GOT:41.3±2.0 unit, GPT:29.3±1.8 unit) had a slightly higher trend than those of HBs antigen negative ones. As the results of this study, when patients were medicated for pulmonary tuberculosis, needles completely sterilized should be used, also tests of HBs antigen had to be done in them, and it was thought that GOT, GPT levels were not largely influenced by occurrance of HBs antigen.

한국산 족부 백선균의 항진균제 감수성에 관한 연구

김승곤,김태전,이태수 서울保健大學 1997 論文集 Vol.17 No.1

Minimum inhibitory concentration(MIC) of griseofulvin, imidazole derivatives such as ketoconazole, econazole, miconazole and itraconazole on 52 strains of Trichophyton ruhrum, 22 strains of T. mentagraphytes, 10 strains of Microsporum spp. and 1 strain of Epidermophyton floccosum were determined after one week of incubation at 25C on Sabouraud dextrose broth. The results were summarized as followes : 1. The MICs of griseofulvin against 4 different tinea pedis fungi were ranged from 10-100mcg/ml. This values indicated that effectiveness of antifungal activity were relatively lower than those of three other imidazole derivatives such as ketoconazole, econazole, miconazole and one itraconazole. 2. Out of three imidazole derivatives, econazole had relatively higher inhibitory concentrations with 0.1-10mcg/ml against all tinea pedis isolated. 3. Out of 5 antifungal agents, itraconazole which is trizole derivatives showed the highest inhibitory activity with 0.1-10mcg/ml against all tinea pedis tested. 4. These results indicated that inhibitory activity of griseofulvin against tinea pedis were lower than those of four other antifungal agents tested. But these four antifungal agents such as ketoconazole, econazole, miconazole and. itraconazole were effective against tinea pedis tested.

토양에서 분리된 방선균들에 의한 Rimazol Brilliant Blue R(RBBR) 염료의 탈색에 질소원과 pH가 미치는 영향

김태전,김승곤,윤경하 서울保健大學 1997 論文集 Vol.17 No.1

Many workers had reported that the degradation of lignocellulose and lignin by white rot fungi were significantily correlative with the degradation of phenol or PAHs (Polycyclic aromatic hydorcarbons) compounds and the decolorization of a various dyes, specially RBBR (Remazol brilliant blue R) and Poly R-478 dyes. but it were not known distinctly that the degradation of these and the decolorization of these by Actinomycetes were related to the degradation of phenol or PAHs compounds, and author had examined in relationship between the degradation of lignocellulose or lignin and the decolorization of RBBR by Actinomycetes isolated in 67 soil samples collected at 8 areas of Korea from February, 1994 to March, 1995. In the experiment, There were selected two strains identified as Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 which had good correlation between the degradation of lignocellulose or lignin, and it were investigated that the cultural parameters to maximize the decolorization ratios of RBBR by two strains, as the results, Streptomyces halstedii scabies SA1-36 revealed each the highest decolorization ratios with 73.4% in 0.30% glycerin and Streptomyces lavendulas SA2-14, with 74.1% in 0.25% cellobiose at 30℃ in 20 days of culture. There were used their results as basal data in this study that examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR. It were summarized as following that the results examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR by two strains. 1) It were the highest each as 75.7% in casein and 75.7% in yeast extract that the dicolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to nitrogen sources(0.l%). 2) It were the highest each as 76.8% in 0.1% casein and 78.3% in yeast extract that the decolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to the concentrations of casein and yeast extract. 3) It were the highest each as 76.8% in pH 7.6 and 79.4% in pH 6.6 that the decolorization ratios of RBBR according to pH by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains. In the above results, we could knowe that it were each different as well as carbon sources that nitrogen sources and pH required between Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2- 14 strains. and organic nitrogens influence more than inorganic nitrogens to decolorize RBBR dye and nitrogen sources and pH influence lower than carbon sources to decolorize RBBR dye. What`s more, we could knowe that it were very important to use newly formuliged optimal basal media maximize decolorization ratios of RBBR dye by Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2-14 strains.

血液 및 尿中 水銀濃度의 統計學的인 比較 檢討

김승곤,김태전 서울保健大學 1986 論文集 Vol.6 No.1

초파리 자연집단의 유해유전자 빈도의 변화에 관한 연구

김승곤,추종길,김태전 서울保健大學 1987 論文集 Vol.7 No.1

고사리(Pteridium aquilinum)뿌리 전분의 이화학적 특성에 관한 연구 : 제2보 : 전분의 물리적 특성 Ⅱ. Physical Properties

曺哉銑,金成坤,李啓瑚,權泰完 경희대학교 부설 식량자원개발연구소 1981 硏究論文集 Vol.2 No.-

고사리뿌리 전분의 이화학적인 특성연구의 일환으로 실시된 물리적 특성시험 결과는 다음과 같다. 전분입자의 미구조의 결합력을 보기 위한 흡수도, 팽운력, 용해도 시험결과 감자전분이나 타피오카전분보다 강한 결합을 하고 있으며 곡류전분보다는 약하며 팽윤력이 single-stage pattern을 이루고 있었다. 가열에 의한 전분입자의 복굴절성 상실, α-아밀라아제에 의한 소화성 및 X-선 회절둥으로 전분의 호화과정을 살펴본 결과 55-60℃에서 호화가 시작되고 60~70℃에서는 95%이상 호화되었다. 또한 3~6%전분용액의 가열에 따른 점도변화를 amyl-ograph를 사용하여 실험한 결과 pasting temperature 62~68℃, 최고점도 80~840BU, 50℃로 냉각후의 점도는 110~555BU였다. 따라서 다른 전분에 비해서 점도가 낮으나 setback이 현저히 느렸다. 수분함량 50%의 전분겔의 경도는 팥 전분보다는 약하고 감자나 밀전분에 비해서는 현저히 높았다. 전분겔의 저장중 노화속도는 밀 전분보다는 느리고 파지오카 전분보다는 빨랐다. 고사리뿌리 전분은 팥 전분겔특성에 큰 변화를 주지않지만 노화를 억제하였고 밀 전분겔에 첨가시에는 겔의 경도가 저하되어 부드러워지는 경향을 보였다. The gelatinization phenomena of bracken root starch were examined by means of the loss of birefringence, degree of digestibility by amylase and X-ray diffraction. These results indicated that gelatinization temperature of the starch was 55∼60℃ and over 95% of starch were gelatinized at the temperature between 60 and 70℃. The swelling power of the bracken root starch was much less steeper than that of potato or tapioca starch. Amylograph data on the various starch concentrations showed the pasting temperature of 62∼68℃, peak height of 80∼840 Brabender unit (BU) and peak after cooling to 50℃ of 110∼555 BU. According to the information obtained from amylograph data, the bracken root starch showed low set back. The rate of retrogradation of the starch as tested by Texturometer was slower and faster than potato and tapioca starches, respectively.

병원내 생리기능 검사실의 적정 업무환경에 관한 연구

배형준,윤기은,김승곤,나동진,문희주,김태전,박정오 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

The purpose of this study was to suggest guideline for optimal work environment of physiological laboratory in hospital. Therefore this study was carried out to analyze the actual condition of laboratory system, major equipments and persons. The period of survey was from September to November 1997 and the objects were 123 laboratory. The major results of this study were as follows. 1. The optimal areas of general ECG room, exercise ECG room and echocardiology room were all 9-18㎡, that of Holter ECG room was 3-12㎡. The optimal areas of neurosystem laboratory were all 9-12㎡, those of respiratory system laboratory were all 9-18㎡. 2. The optimal number of persons laboratory of hospital 500 beds and less were 2∼3, those of 501∼700 beds were 3∼4.701∼1.000 beds were 4∼5 and over 1.001 beds were 7∼8 persons in circulatory system laboratory. The optimal number of persons laboratory of hospital 500 beds less than were 1∼2, those of 501∼700 beds were 3∼4, 701∼1.000 beds were 6∼7 and over 1.001 beds were over 8 persons in neurosystem laboratory. The optimal number of persons laboratory of hospital 500 beds and less 1. those 501∼1.000 beds were 1∼2 and over 1.001 beds were 2∼3 persons in repiratory system laboratory. 3. The optimal number of equipments of general ECG were 2∼3 in the 500∼1.000 beds hospital. there were 3∼4 units in the 1,001 beds. The optimal number of equipment EEG were both 1∼2 in the hospital 500 beds and less and 501∼700 beds. those were 4∼5 units in hospital over 701 beds. The optimal number of equipments of spirograph were both 1∼2 in the hospital 500 and less and 501∼1.000 beds. those were 2∼3 units in the over 1.001 beds. 4. In view of speciality and legal problem of physiofunctional test. we sugget that non-licensee were so quickly change place licensee in the interests of optimal work environment. 5. We suggest that to upgrade qualitative level of domestic equipment and to the more amplify utilization with respect to domestic equipment for the purpose of reducing loss of foreign money.

尿中 Aminolevulinic Acid와 Porphobilinogen의 비교 조사 연구

나동진,윤기은,김승곤,문희주,김태전,박정오 서울保健大學 1990 論文集 Vol.10 No.1

This studies were examined to measure total ALA and PBG concentration in Urine, and find the relationship of correlation among them in 57 men unexposed and 43 men exposed by mercury. The results were summarized as the following; 1. In 100 populations total urine ALA concentration was 0.85±0.17㎎% in male, in female were 0.40 ± 0.04㎎% (normal value : 0.54㎎%). 2. Urine ALA concentration in male were 1.04∼1.11mg% for the ages between 30 ∼ 49 was the highest and then in female were 0.58㎎% for the ages between 40 - 59 was the highest. 3. In 100 populations total urine PBG concentration was 0.03㎎% in male, in female were 0.02㎎% (normal value : 0.03㎎%). 4. Urine PBG concentration in male were 0.05㎎% for the ages between 30-49 was the highest. 5. In unexposed men group total urine ALA concentration was 0.13±0.03㎎% in male, in female were 0.11±0.0l㎎%. 6. In exposed men group total urine ALA concentration was 1.81±0.55㎎% in male, ill female were 0.78±0.09㎎%, there was differences in attention showing male was higher than the female (P<0.05). 7. In unexposed men group total urine PBG concentration was 0.02㎎% in male, in female were 0.0l㎎%. 8. In exposed men group total urine PBG concentration was 0.06㎎% in male, in female were 0.04㎎%,there was differences in attention showing male was higher than the female (P<0.05).

供血者에서의 HBsAg 發現率과 生化學的 檢査値에 관한 硏究

문희주,윤기은,김승곤,라동진,김태전,박정오,최범열 서울保健大學 1988 論文集 Vol.8 No.1

임상검체에서 분리된 Serratia sp. 2000-1에 의한 Protease의 생산 및 효소학적 성질

김태전(Tai Jeon Kim),김승곤(Sung Kon Kim),김상택(Sang Taek Kim) 대한의생명과학회 2000 Biomedical Science Letters Vol.6 No.3

본 논문은 환자들의 병소에서 채취된 가검물에 존재하는 미생물들 가운데 protease을 분비하는 균주들을 분리하고, 그 가운데 가장 효소생산력이 우수한 균주를 선정하여 가정용 Protease 세제생산에 활용 가능성을 알아보고자 그들이 생산하는 protease의 기초적 생산조건 및 부분적 효소학적 특성 등에 관한 실험에서 얻은 결과들을 보고하고자 작성되었다. Serratia sp. 2000-1로 동정된 본 실험 균주가 생산하는 protease의 기초적 생산조건과 부분적 효소학적 특성을 조사한 결과 효소생산을 위한 최적 배지에 탄소원과 질소원 그리고 금속염의 종류와 농도는 각각 Glucose 1.5%, C.S.P 2.0%, CaCl₂ 0.1%였다. 그리고 최적 배양온도는 30℃, 초발 pH는 8.0, 배양시간은 72시간 이었다. Protease의 정제는 ammonium sulfate precipitation, DEAE-cellulose, Sephadex G-200 column을 통하여 분리 정제하였으며 이때 최종 효소수율은 14.4%, 효소 비활성도는 약 29배 증가한 것으로 나타났다, 그리고 효소 작용의 최적온도와 pH는 35℃와 pH 8.0으로 나타났고, 효소 작용의 열과 pH에 대한 안정성은 40℃와 pH 6~10까지는 효소의 활성이 비교적 안정한 것으로 나타났다. 금속이온들 중 Mg²?, Ba²?, Ca²?, Mn²?은 효소활성을 촉진하나 Hg²?, Ag²?, Cu²?는 효소활성을 저해하는 것으로 나타났다. 그리고 효소활성 저해제들 중에는 SDS가 가장 강하게 효소활성을 저해하는 것으로 나타났다. The purpose of this study was to investigate the practical availability of protease production that can be used at home after isolating Serratia sp. 2000-1 which produced extracellular protease from clinical specimen. Basic production conditions and partial enzymatic characteristics of protease produced by Serratia sp. 2000-1 was as follows: The kind and concentration of carbohydrate, nitrogen and metal salts for optimal enzyme production condition were each identified as the concentration of 1.5% glucose, 2.0% CSP, and 0.1% CaCl₂, and the optimal temperature, time and initial pH for culture were each 30℃, 72 hours, and pH 8.0. The final enzymatic yeild that was purified by 3 steps with ammonium sulfate precipitation (45~80%), DEAE-cellulose column chromatography, and Sephadex G-200 gel chromatography was 14.4%, and enzyme inactivity rate increased approximately 29 folds. The optimal temperature and pH for purified protease activity were 35℃ and pH 7.0~8.0, and purified protease activity was relatively stable by 40℃ at pH 6~10 for 30 min, however heating at 60℃ for 30 min, it liminated detectable protease activity. The protease activity was activated by Mg²?, Ba²?, Ca²?, Mn²?, but inactiviaed by Hg²?, Ag?, Cu²?, and the protease activity was inhibited strongly by SDS among enzyme activity inhibitors. Further study is required to evaluate the practical availability of protease production that can be used at home by isolating Serratia sp. from more clinical specimen and examining protease more in details.