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Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.
Expression of human β‐defensin‐2 in the prostate
Kim, Hae Jong,Jung, Ja Rang,Kim, Hea Jin,Lee, Shin Young,Chang, In Ho,Lee, Tae Jin,Kim, Wonyong,Myung, Soon Chul Blackwell Publishing Ltd 2011 BJU INTERNATIONAL Vol.107 No.1
<P>We found the expression of human beta-defensin-2 (HBD-2) in the prostate for the first time and LPS, a gram negative bacterial component, upregulated HBD-2 in prostate epithelial cells. We are looking for other antimicrobial peptides expressed in the prostate besides human beta-defensin-2. Also, we are studying the relationship between antimicrobial peptides and the development or progression of prostate diseases. OBJECTIVE To investigate the expression and regulation of human beta-defensin-2 (HBD-2) in the prostate. PATIENTS AND METHODS Normal human prostate epithelial cell line (RWPE-1), human prostate cancer cell lines (DU-145, PC-3), and paraffin-embedded prostate tissue from patients with benign prostatic hyperplasia (BPH) were analysed by RT-PCR and immunohistochemical staining. HBD-2 expression was also analysed by RT-PCR and ELISA in RWPE-1 cells treated with lipopolysaccharide (LPS). Nuclear factor-kappa B (NF-kappa B) activation was assessed by I kappa B alpha immunoblotting and electrophoretic mobility shift assay (EMSA). RESULTS BPH tissue and all of the tested prostate cell lines other than PC-3 constitutively express HBD-2 mRNA. HBD-2 protein was strongly detected in prostate gland tissue surrounded by inflammatory cells including macrophages. Exposure to LPS induced HBD-2 upregulation and NF-kappa B activation, as assessed by I kappa B alpha phosphorylation and degradation in RWPE-1 cells. Bay11-7082, an NF-kappa B inhibitor prevented LPS-induced HBD-2 production in RWPE-1 cells. CONCLUSIONS Prostate epithelial cells may constitutively express HBD-2, and its expression was upregulated by LPS. Our data indicate that HBD-2 may be an important immunomodulatory factor in prostate function. Expression of HBD-2 in normal prostates and the potential role of HBD-2 in prostatitis and BPH should be addressed in the future.</P>
Evaluation of Pesticides Residues in Agricultural Products for Raw Juice in Seoul
Tae-rang Kim,Young-hee Choi,Mi-sun Kim,Young-hee Jin,Mi-ok Song,Eun-jung Han,Chun-yeoung Lee,Hee-jin Choi,Min-jung Kim,Hyun-Jeong Kim,Eun-sun Yun,Yong-Seung Shin 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
In this study, we conducted the levels of pesticide residue as well as the risk assessment for pesticides which exceeded maximum residue limits(MRLs). A total of 345 Agricultural Products (239 Vegetables and 106 Fruits) for raw juice were distributed from the southern area of Seoul from January to August in 2021 for 280 types of pesticides were analyzed by multi-residue method in Korean Food Code. As a result, Occurrences of violation Max Residue Limits(MRLs) of pesticide residues were found in the 4 samples(3 kales and 1 celery, 1.2%). However, the levels of residual pesticides in Fruits were not exceeded MRLs. Pesticide residue levels in 60 cases(33 fruits and 27 vegetables, 17.4%) reveal to the below the standard limits. The detection rate of pesticides in fruits for juice was 31.1%, which was about three times higher than the 11.3% of vegetables. The most commonly exceeded their MRLs pesticides residues were three types of pesticides(paclobutrzol, fluopyram and vinclozolin). The safety levels of these residual pesticides that exceeded MRLs was assessed using the ratio of estimated daily intake(EDI) and acceptable daily intake(ADI). The results showed that their ratios were between 0.34~1.43% of ADI, which indicated that these pesticides were within the safe limits.
Tae Rang Kim,Su Un Kim,Young Shin,Ji Young Kim,Sang Me Lee,Jung Hun Kim 한국식품영양과학회 2013 Preventive Nutrition and Food Science Vol.18 No.4
In this study, the quick HPLC-MS/MS method for the simultaneous separation of 2-acetyl-4(5)-tetrahydroxybuthylimidazole (THI) and 4-(5)-methylimidazole (4-MI) in alkaline medium was used for caramel color and processed foods in Korea. After a simple sample pretreatment, 51 4-MI-labeled samples were positive for 4-MI and 2 also contained THI. The concentration of 4-MI was 260.5∼24,499.3 μg/kg in caramel color, less than LOD∼1,712.5 μg/kg in sauce, 1,242.3, 5,972.2 μg/kg in balsamic vinegar, 2,118.3∼5,802.4 μg/kg in complex seasoning, 82.7∼5,110.6 μg/kg in curry, and 29.9∼464.4 μg/kg in soft drinks. The recovery rate of 4-MI was 97.1∼111.0% in sauce and 81.9∼110.0% in powder and that of THI was 83.6∼106.4% in sauce and 61.2∼99.4% in powder. Our results concluded a safe amount of 4-MI and THI compared to the limit of Korea additive code but the processed foods do not have a limit of caramel color and 4-MI in Korea. Therefore, research and monitoring of 4-MI and THI is needed for processed foods in Korea.
Kim, Tae Rang,Kim, Su Un,Shin, Young,Kim, Ji Young,Lee, Sang Me,Kim, Jung Hun The Korean Society of Food Science and Nutrition 2013 Preventive Nutrition and Food Science Vol.18 No.4
In this study, the quick HPLC-MS/MS method for the simultaneous separation of 2-acetyl-4(5)-tetrahydroxybuthylimidazole (THI) and 4-(5)-methylimidazole (4-MI) in alkaline medium was used for caramel color and processed foods in Korea. After a simple sample pretreatment, 51 4-MI-labeled samples were positive for 4-MI and 2 also contained THI. The concentration of 4-MI was 260.5~24, $499.3{\mu}g/kg$ in caramel color, less than $LOD{\sim}1,712.5{\mu}g/kg$ in sauce, 1,242.3, $5,972.2{\mu}g/kg$ in balsamic vinegar, $2,118.3{\sim}5,802.4{\mu}g/kg$ in complex seasoning, $82.7{\sim}5,110.6{\mu}g/kg$ in curry, and $29.9{\sim}464.4{\mu}g/kg$ in soft drinks. The recovery rate of 4-MI was 97.1~111.0% in sauce and 81.9~110.0% in powder and that of THI was 83.6{\sim}106.4% in sauce and 61.2{\sim}99.4% in powder. Our results concluded a safe amount of 4-MI and THI compared to the limit of Korea additive code but the processed foods do not have a limit of caramel color and 4-MI in Korea. Therefore, research and monitoring of 4-MI and THI is needed for processed foods in Korea.
Kim, Young Jong,Park, Hae Jeong,Jahng, Geon-Ho,Lee, Sang Min,Kang, Won Sub,Kim, Su Kang,Kim, Tae,Cho, Ah Rang,Park, Jin Kyung Elsevier 2017 Psychiatry Research Vol.255 No.-
<P><B>Abstract</B></P> <P>Suicide is a serious public health problem. In this study, we investigated functional brain changes to novel visual stimuli of suicidal means, DNA methylation status, and the relationship between the two markers. 14 suicidal attempt patients (SAs) and 22 healthy controls were included. Pictures of facial expressions and suicidal means were shown to subjects during fMRI scanning. 11 CpG sites within transcription factor binding site of CACNA1C gene were selected. In knives (K) vs. natural landscape (NL) condition, left middle frontal gyrus (Brodmann's area, BA 6 and 46) and left inferior frontal gyrus (BA 9) were shown to be significantly higher brain activation in the SAs than the controls (p<0.001). DNA methylation percentages of CpG site 4 (p=0.005) and 6 (p=0.037) were found to be related to the SAs. In the SAs, methylation degree of site 4 and site 6 was positively correlated with signal intensity of K vs. NL condition in left thalamus. The degree of site 4 was positively correlated with signal intensity in left middle and inferior frontal gyri in SAs. The possibility that these findings might be involved in the neurobiology of suicidal behavior is suggested.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Functional brain change and DNA methylation were investigated in suicidal attempters. </LI> <LI> Distinct brain activation and DNA methylation were found in suicidal attempters. </LI> <LI> The possibility of a neurobiological evidence for suicide is suggested. </LI> </UL> </P>