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종합병원 간호사의 직무스트레스와 근골격계 증상과의 관련성
김영실(Kim Young Sil1),박재용(Park Jae-Yong),박상연(Park Sang-Youn) 대한근관절건강학회 2009 근관절건강학회지 Vol.16 No.1
Purpose: The purpose of this study was to investigate the relationships between job stress and work-related musculo-skeletal symptoms among nurses working in general hospitals. Method: A descriptive correlational research design was utilized. The participants were 438 nurses currently working in 4 general hospitals. Data were collected from Jan 2007 to Feb 2007, with Korean Occupational Stress Scale (KOSS)-basic form, KOSHA code H-30 and a questionnaire for general characteristics. Descriptive statistics, t-test, ANOVA, multiple logistic regression analyses were utilized. Result: Most participants (90.4%) complained of work-related musculo-skeletal symptoms. However, symptomatic nurses satisfying NIOSH screening criteria (symptomatic nurses) were 66.9% (293 persons). The most complained body part was back (39.5%). shoulder (37.7%), leg and foot (36.5%), wrist and hand (21.7%), neck (18.7%), and arm (9.8%). The mean of total score of occupational stress was 51.11. In multiple logistic regression analyses, age, working posture and total score of occupational stress showed significant associations with back symptoms. Exercise and total score of occupational stress were significantly related with shoulder symptoms. Hours of daily house keeping and working posture were related with leg and foot symptoms. Marital status and working posture were related with wrist and hand symptoms. Working posture only showed significant relations with neck symptom. Marital status, hours of daily house keeping, shift work, working posture, and total score of occupational stress were related with the symptoms in the arm. In conclusion, job stress of nurses might be related with work-related musculo-skeletal symptoms.
Sung, Dong Kyung,Chang, Yun Sil,Sung, Se In,Yoo, Hye Soo,Ahn, So Yoon,Park, Won Soon John Wiley and Sons Inc. 2016 Cellular microbiology Vol.18 No.3
<P><B>Summary</B></P><P>Recently, we demonstrated that intratracheal transplantation of human umbilical cord blood‐ derived mesenchymal stem cells (MSCs) attenuates <I>Escherichia (E) coli</I>‐ induced acute lung injury primarily by down‐ modulating inflammation and enhancing bacterial clearance iQn mice. This study was performed to elucidate the mechanism underlying the antibacterial effects of MSCs. The growth of <I>E. coli in vitro</I> was significantly inhibited only by MSCs or their conditioned medium with bacterial preconditioning, but not by fibroblasts or their conditioned medium. Microarray analysis identified significant up‐ regulation of toll‐ like receptors (TLR)‐ 2 and TLR‐ 4, and β‐ defensin 2 (BD2) in MSCs compared with fibroblasts after <I>E. coli</I> exposure. The increased BD2 level and the <I>in vitro</I> antibacterial effects of MSCs were abolished by specific antagonist or by siRNA‐ mediated knockdown of TLR‐ 4, but not TLR‐ 2, and restored by BD2 supplementation. The <I>in vivo</I> down‐ modulation of the inflammatory response and enhanced bacterial clearance, increased BD2 secretion and the resultant protection against <I>E. coli</I>‐ induced pneumonia observed only with MSCs, but not fibroblasts, transplantation in mice, were abolished by knockdown of TLR‐ 4 with siRNA transfection. Our data indicate that BD2 secreted by the MSCs via the TLR‐ 4 signalling pathway is one of the critical paracrine factors mediating their microbicidal effects against <I>E. coli</I>, both <I>in vitro</I> and <I>in vivo</I>. Furthermore, TLR‐ 4 from the transplanted MSCs plays a seminal role in attenuating <I>in vivo E. coli</I>‐ induced pneumonia and the ensuing acute lung injury through both its anti‐ inflammatory and antibacterial effects.</P>
Combinatory treatment using tacrolimus and a STAT3 inhibitor regulate Treg cells and plasma cells
Park, Jin-Sil,Kim, Sung-Min,Hwang, Sun-Hee,Choi, Si-Young,Kwon, Ji Ye,Kwok, Seung-Ki,Cho, Mi-La,Park, Sung-Hwan SAGE Publications 2018 International journal of immunopathology and pharm Vol.32 No.-
<P>Systemic lupus erythematosus (SLE; lupus) is a prototypical autoimmune disease characterized by circulating autoantibodies to nuclear antigens and immune complex deposition, resulting in damage to target organs. To investigate the effects of tacrolimus (TAC) on effector T cells and B cells, we examined its involvement in the development of effector T cells, germinal center (GC) B cells, and plasma cells in an in vitro system using wild-type (WT) and lupus-prone mice. The population of T helper (Th) 1, Th2, and Th17 cells interleukin (IL)-17-producing T (Th17) cells and the production of interferon-γ and interleukin-17A IL-17A were suppressed by TAC. TAC also reduced the population of regulatory T (Treg) cells; however, a combination treatment with the signal transducer and activator of transcription 3 (STAT3) inhibitor STA-21 promoted the population of Treg cells. TAC also suppressed the populations of GC B cells and plasma cells synergistically with STA-21. These findings suggest that the application of TAC with a STAT3 signal inhibitor may provide benefits in SLE treatment.</P>
Regulator of Calcineurin 3 Ameliorates Autoimmune Arthritis by Suppressing Th17 Cell Differentiation
Park, Jin-Sil,Jeong, Jeong-Hee,Byun, Jae-Kyeong,Lim, Mi-Ae,Kim, Eun-Kyung,Kim, Sung-Min,Choi, Si-Young,Park, Sung-Hwan,Min, Jun-Ki,Cho, Mi-La Elsevier 2017 The American journal of pathology Vol.187 No.9
<P>Regulator of calcineurin 3 (RCAN3), an endogenous regulator of the calcineurin nuclear factor of activated T cells (NFAT) signaling pathway, inhibits the phosphatase activity of calcineurin, the nuclear translocation of NFAT, and the NEAT downstream pathway. To investigate the effects of RCAN3 on T-cell regulatory function and the development and progression of inflammatory arthritis, we studied the effects of RCAN3 transfection on regulation of Th17 cell differentiation in a murine T-lymphoma cell line and primary splenic CD4+ T cells. Overexpression of RCAN3 suppressed Th17 cell differentiation through the down-regulation of RAR receptor orphan receptor gamma T mRNA and up-regulation of forkhead box P3 mRNA. In mice with collagen-induced arthritis, injection of an RCAN3-overexpression vector controlled arthritis development in vivo. Injection of RCAN3 reduced the formation of osteoclasts and expression of inflammatory cytokines in vivo. Antioxidants stimulated the expression of RCAN3 in vitro, and combination therapy with pcDNA-RCAN3 had a synergistic suppressive effect on the development of arthritis. These data suggest that RCAN3 may be an effective treatment for rheumatoid arthritis.</P>
Antioxidant Effects of Spinach (Spinacia oleracea L.) Supplementation in Hyperlipidemic Rats
Sang-Heui Ko,Jae-Hee Park,So-Yun Kim,Seon Woo Lee,Soon-Sil Chun,Eunju Park 한국식품영양과학회 2014 Preventive Nutrition and Food Science Vol.19 No.1
Increased consumption of fresh vegetables that are high in polyphenols has been associated with a reduced risk of oxidative stress-induced disease. The present study aimed to evaluate the antioxidant effects of spinach in vitro and in vivo in hyperlipidemic rats. For measurement of in vitro antioxidant activity, spinach was subjected to hot water extraction (WE) or ethanol extraction (EE) and examined for total polyphenol content (TPC), oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA), and antigenotoxic activity. The in vivo antioxidant activity of spinach was assessed using blood and liver lipid profiles and antioxidant status in rats fed a high fat-cholesterol diet (HFCD) for 6 weeks. The TPC of WE and EE were shown as 1.5±0.0 and 0.5±0.0 ㎎ GAE/g, respectively. Increasing the concentration of the extracts resulted in increased ORAC value, CAA, and antigenotoxic activity for all extracts tested. HFCD-fed rats displayed hyperlipidemia and increased oxidative stress, as indicated by a significant rise in blood and liver lipid profiles, an increase in plasma conjugated diene concentration, an increase in liver thiobarbituric acid reactive substances (TBARS) level, and a significant decrease in manganese superoxide dismutase (Mn-SOD) activity compared with rats fed normal diet. However, administration of 5% spinach showed a beneficial effect in HFCD rats, as indicated by decreased liver TBARS level and DNA damage in leukocyte and increased plasma conjugated dienes and Mn-SOD activity. Thus, the antioxidant activity of spinach may be an effective way to ameliorate high fat and cholesterol diet-induced oxidative stress.
Sung, Nayoung,Han, Ae Ra,Park, Chan Woo,Park, Dong Wook,Park, Joon Cheol,Kim, Na Young,Lim, Kyung Sil,Shin, Ji Eun,Joo, Chang Woo,Lee, Seung Eun,Kim, Jae Won,Lee, Sung Ki,IVIG Task Force Korean Societ The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.1
The task force of the Korean Society for Reproductive Immunology recommends intravenous immunoglobulin G treatment in women with reproductive failure, including recurrent pregnancy loss and/or repeated implantation failure, who show cellular immune factors such as abnormal natural killer cell levels, natural killer cell cytotoxicity, and/or type 1 T helper immunity.
Inhibitoin of Human Malignant Glioma Growth In Vivo by Human Recombinant Plasminogen Kringles 1-3
Park,Chun Kun,Joe,Kyung Keun,Kim,Moon Chan,Joe,Young A,Son,Byung Chul,Jeun,Shin Soo,Yang,Youn Joo,Kang,Joon Ki,Hong,Yong Kil,Lee,Kwan Sung,Lee,Youn Soo,Lee,Hyo Sil,Chung,Soo Ii,Chung,Dong Sup 가톨릭중앙의료원 가톨릭암센터 1999 암심포지움 Vol.- No.2
Human malignant gliomas are highly vascularized and aggressive tumors. Angiogenesis inhibitors have shown to induce regression of a variety of primary and metastatic tumors in vivo. However, their usefulness in treating brain tumors is not wellunderstood. Angiostatin, a multiple kringle (1-4 out of 5) containing fragment of plasminogen, is one of the highly effective natural cryptic angiogenesis inhibitors. In this study, the theraperutic efficacy of a non-glycosylated and small molecular size recombinant kringles 1-3(rPK1-3) was examined in the treatment of brain tumors generated by stereotactic intracerebral implantation of U-87 human glioma cells in nude mice. Mice bearing tumors, post-7 day implant, were treated with rPK1-3(100 mg/kg/day) s.c. for 21 days. The treated animals showed suppressed brain tumor growth by greater than 71.2% along with a 3-fold increase of apoptotic index and suppressed vascularization by 78.9%, without any observable signs of toxicity. Analysis of bFGF and VEGF expression in the tumors of the treated animals using immunohistochemical methods showed near complete absence of growth factors. Our results indicate that a non-glycosylated, small molecular size rPK1-3 is an efficient tumoristatic agent for the treatment of intracranial human glioma wenograft in mice and might provide ew strategies for the treatment of brain tumors.
The Influences of Extremely Low Frequency Magnetic Fields on Drug-Induced Convulsion in Mouse
Sung, Ji-Hyun,Jeong, Ji-Hoon,Kim, Jeong-Soo,Choi, Tai-Sik,Park, Joon-Hong,Kang, Hee-Yun,Kim, Young-Sil,Kim, Dong-Suk,Sohn, Uy-Dong The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.6
This study investigated the effects of extremely low frequency magnetic fields (ELF-MFs) on the sensitivity of seizure response to bicuculline, picrotoxin and NMDA in mice. The mice were exposed to either a sham or 20 G ELF-MFs for 24 hours. Convulsants were then administered i.p. at various doses. The seizure induction time and duration were measured and lethal dose ($LD_{50$}) and convulsant dose ($CD_{50}$) of the clonic and tonic convulsion were calculated. The analysis of glutamate, glycine, taurine and GABA of mouse brain was accomplished by HPLC. The mice exposed to ELF-MFs showed moderately higher $CD_{50}.{\;}LD_{50}$ and onset time on the bicuculline-induced seizure. However, the ELF-MFs did not influence them in the NMDA and picrotoxin-induced seizures. After the exposure to MFs exposure, the glutamate level was increased and GABA was decreased significantly in NMDA and picrotoxin-induced seizure. The level of glutamate and GABA were not changed by MFs in bicuculline-induced seizure. These results suggest that ELF-MFs may alter the convulsion susceptibility through GABAergic mechanism with the involvement of the level of glutamate and GABA.
Multiple births conceived by assisted reproductive technology in Korea
Park, Young-Sil,Choi, Sun-Hee,Shim, Kye-Shik,Chang, Ji-Young,Hahn, Won-Ho,Choi, Yong-Sung,Bae, Chong-Woo The Korean Pediatric Society 2010 Clinical and Experimental Pediatrics (CEP) Vol.53 No.10
Purpose: The recent trends of multiple births (MBs) conceived by assisted reproductive technology (ART) in Korea were analyzed as well as the relationship with maternal age, especially advanced maternal age. Methods: Data were obtained from the Korean Statistical Information Service and annual ART reports from the ART committee of the Korean Society of Obstetrics and Gynecology. Results: MBs increased from the early 1990s; there was a 275% increase by 2008. The number of total live births was 448,153 and MBs accounted for 10,767; the MB rate was 24.0% in 2006. Among 2,326 deliveries conceived by ART, multiple deliveries accounted for 786 (33.8%). The total number of live births with ART was 3,125 and 1,585 (50.7%) of them were MBs. During 2006, 14.7% of the entire MBs in Korea were associated with ART. The proportion of women of advanced maternal age was much higher in the ART group than in the total live birth group. Conclusion: MBs in women of advanced maternal age have been increasing in Korea with the use of ART. The results of this study showed that ART was a significant factor associated with the increase in MBs in Korea.