http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kwon, Tack-Min,Yi, Young-Byung,Nam, Jae-Sung The Korean Society of Plant Biotechnology 2011 식물생명공학회지 Vol.38 No.4
The CCR4-CAF1-NOT complex-mediated degradation of mRNA is a fundamental aspect of gene regulation in eukaryotes. We herein examined the role of AtCAF1 in the innate immune and wound responses of plants. Our results showed that overexpression of AtCAF1 significantly downregulated the transcript level of EFR but not FLS2 and BRI1, as well as abolished up-regulated expression pattern of EFR in response to wounding. Consistently, Agrobacteriummediated transient expression of GUS was highly enhanced in the transgenic plants overexpressing AtCAF. Furthermore, JA responsive genes were down-regulated by overexpression of AtCAF, causing the transgenic plants overexpressing AtCAF more susceptible to necrotrophic fungal pathogen, Botrytis cinerea. These results suggest that The CCR4-CAF1-NOT complex-mediated degradation of mRNA negatively regulates wounding-mediated disease resistance in Arabidopsis thaliana.
Nanotube-on-graphene heterostructures for three-dimensional nano/bio-interface
Kwon, Sun Sang,Shin, Jae Hyeok,Choi, Jonghyun,Nam, SungWoo,Park, Won Il Elsevier 2018 Sensors and actuators. B Chemical Vol.254 No.-
<P><B>Abstract</B></P> <P>We report the synthesis, fabrication, and characterization of a nanotube-on-graphene (NT-on-Gr) field-effect sensor array for electrical detection of the biological activity of living cells. In order to form vertical nanotubes on a graphene surface, Ge/Si core-shell nanowires were vertically grown on graphene, followed by cap opening and Ge core-etching processes. Source-drain current versus water-gate potential measurements in electrolyte solutions with various pH values showed typical gate-dependent ambipolar characteristics with a decrease in pH sensitivity versus that of a flat graphene field-effect sensor. This is associated with limited solution gating of Si nanotubes that form nanoscale fluidic channels and thus interconnect the solution with the graphene field-effect sensor. The Si nanotubes also bridged interconnections between cells and the graphene field effect sensors, which were then able to record electrical spike peaks caused by cell networks.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The nanotube-on-graphene heterostructure devices were fabricated for sensor application. </LI> <LI> Investigate the ability to record cellular electrical activity using nanotube-on-graphene heterostructure devices. </LI> <LI> Demonstrate the potential of the multiplexed electrical recording capabilities of biological sensor arrays based on vertical nanotubes and planar graphene FETs. </LI> </UL> </P>
Sang Yoon Lee,Ki Hong Kim,Yoon Kwon Nam 한국수산학회 2009 Fisheries and Aquatic Sciences Vol.12 No.2
The cytoskeletal β-actin gene and its 5`-upstream region were isolated and characterized in the rockbream (Oplegnathus fasciatus). Complementary DNA of the rockbream β-actin represented a 1,125 bp of an open reading frame encoding 375 amino acids, and the rockbream β-actin cDNA and deduced amino acid sequences were highly homologous to those of other vertebrate orthologs. At the genomic level, the β-actin gene also exhibited an organization typical of vertebrate cytoskeletal actin genes (2,159 bp composed of five translated exons interrupted by four introns) with a conserved GT/AG exon-intron splicing rule. The putative non-translated exon predicted in the rockbream β-actin gene was much more homologous with those of teleostean β-actin genes than those of mammals. The 5`-upstream regulatory region isolated by genome walking displayed conserved and essential elements such as TATA, CArG and CAAT boxes in its proximal part, while several other immune- or stress-related motifs such as those for NF-kappa B, USF, HNF, AP-1 and C/EBP were in the distal part. Semi-quantitative RT-PCR assay results demonstrated that the rockbream β-actin transcripts were ubiquitously but different-tially expressed across the tissues of juveniles.
Effects of Desalinization Management on Rice Yield in Sea Water Flooded Field
Sang-Su Kim,Won-Ha Yang,Weon-Young Choi,Hong-Kyu Park,Min-Gyu Choi,Nam-Hyun Back,Si-Yong Kang,Hyun-Tak Shin,Soo-Yeon Cho,Seog-Ju Kwon,Bok-Rae Ko 韓國作物學會 1999 Korean journal of crop science Vol.44 No.1
Over 2,000 ha of rice fields in the western and southern coastal region of Korea were flooded with sea water during the spring tide, on August 19-21, 1997, and the rice plant at heading stage was injured. The field surveys were undertaken at the sea water flooded paddy fields in Chonbuk Province, to identify the injury symptoms and rice yield damage subjected to different flooding condition and desalinization methods. Five days after sea water flooding at heading stage, the flag leaves of rice plants flooded with 30 ㎝ deep sea water withered from the tip, the withering progressed to the lower leaves in deeper flooding. The spikelets were spotted black and discolored from the tip at 50 ㎝ deep flooded rice, and some panicles changed to white at 80 ㎝ deep flooded rice. Most of the rice leaves submerged completely for an hour were withered and most of panicles changed to white. The milled rice yield, percentage of ripened grain, and 1000 grain weight of flooded rice decreased with deeper flooding water, higher water salinity and longer flooding time. Even under the same flooding conditions, the damage of rice yield varied with the growth stage: heading stage>dough stage>booting stage. Rice yield damage was less in the fields on the upper riverside than those of the fields on the estuary and seaside, because of lower water salinity. In a flooded field, the rice yield damages were reduced as the distance increased from the levees where the sea water inflowed and increased as the distance increased from the fresh water irrigation gate. The desalinization treatments consisting of frequent exchange of irrigation water and spraying with fresh water soon after flooding effectively reduced the rice yield damage.