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Altered Calcium Current of the Vascular Smooth Muscle in Renal Hypertension
Sang Chae Nam,Hye Jeon Jeong,Wonjae Kim,JongUn Lee 대한생리학회-대한약리학회 1999 The Korean Journal of Physiology & Pharmacology Vol.3 No.3
<P> The present study was aimed at investigating whether the calcium current in the vascular smooth muscle (VSM) cells is altered in renal hypertension. Two-kidney, one clip (2K1C) and deoxycorticosterone acetate (DOCA)-salt hypertension were made in Sprague-Dawley rats. Rats without clipping the renal artery or implanting DOCA were used as control for 2K1C and DOCA-salt hypertension, respectively. Four weeks after clipping, systolic blood pressure was significantly higher in 2K1C rats than in control (192⁑24 and 119⁑4 mmHg, respectively, n=16 each). DOCA-salt rats also showed a higher blood pressure (180⁑15 mmHg, n=18) compared with control (121⁑6 mmHg, n=14). VSM cells were enzymatically and mechanically isolated from basilar arteries. Single relaxed VSM cells measured 5∼10 μm in width and 70∼150 μm in length were obtained. VSM cells could not be differentiated in size and shape between hypertensive and normotensive rats under light microscopy. High-threshold (L-type) calcium currents were recorded using whole-cell patch clamp technique. The amplitude of the current recorded from VSM cells was larger in 2K1C hypertension than in control. Neither the voltage-dependence of the calcium current nor the cell capacitance was significantly affected by 2K1C hypertension. By contrast, the amplitude of the calcium current was not altered in DOCA-salt hypertension. These results suggest that high-threshold calcium current of the VSM cells is altered in 2K1C hypertension, and that calcium channel may not be involved in calcium recruitment of VSM in DOCA-salt hypertension.
Trilateral Trade and Taking a Side Between the U.S. and China
( Wonjae Hwang ),( Randy Willemain ),( Sang-hwan Lee ) 한국국방연구원 2020 The Korean Journal of Defense Analysis Vol.32 No.4
Does China’s growing economic power generate political influence over its economic partners’ foreign policies? Does this tendency, if it exists, occur at the expense of U.S. interests on those issues? Since China and the U.S. maintain extremely opposing positions over diverse foreign policy issues, it is important to explore these questions. In the analysis of panel data on trilateral trade for China and the U.S. between 1991 and 2014, our empirical results show that China’s trade partners are likely to vote alongside it but against the U.S., even on human rights or important issues identified by the U.S. government in the UN General Assembly. In both cases, as the relative size of trade with China in comparison to the size of trade with the U.S. increases, a state tends to vote alongside China rather than the U.S. The findings imply that growing policy cooperation between China and its trade partners comes at the expense of U.S. national interests and its leadership to a great extent.
Wonjae Hwang,Seong Min Kang,Sang Yoon Lee,Han Gil Seo,박윤길,Bum Sun Kwon,Kwang Jae Lee,김덕용,김형섭,Shi-Uk Lee 대한재활의학회 2022 Annals of Rehabilitation Medicine Vol.46 No.4
Objective To evaluate the efficacy and safety of Daewoong botulinum toxin type A (NABOTA) after its launch in South Korea.Methods This prospective, multicenter, open-label phase IV clinical trial included 222 patients with stroke. All patients visited the clinic at baseline and at weeks 4, 8, and 12 after injection of upto 360 units of NABOTA into the wrist, elbow, and finger flexor muscles at the first visit. The primary outcome was the change in Modified Ashworth Scale (MAS) score for the wrist flexor muscles between baseline and week 4. The secondary outcomes were the changes in MAS, Disability Assessment Scale (DAS), and Caregiver Burden Scale (CBS) scores between baseline and each visit, and the Global Assessment Scale (GAS) score at week 12.Results There was a statistically significant decrease in the MAS score for the wrist flexors between baseline and week 4 (-0.97±0.66, p<0.001). Compared with baseline, the MAS, DAS and CBS scores improved significantly during the study period. The GAS was rated as very good or good by 86.8% of physicians and by 60.0% of patients (or caregivers). The incidence of adverse events was 14.4%, which is smaller than that in a previous trial.Conclusion NABOTA showed considerable efficacy and safety in the management of upper limb spasticity in stroke patients.
Sang Chae Nam,Seongsu Kang,Wonjae Kim,Jongun Lee 대한생리학회-대한약리학회 2000 The Korean Journal of Physiology & Pharmacology Vol.4 No.1
<P> The present study was conducted to investigate the possible role of the sympathetic nervous system in two-kidney, one clip (2K1C) and deoxycorticosterone acetate (DOCA)-salt hypertension. 2K1C and DOCA- salt hypertension were made in Sprague-Dawley rats. Four weeks after induction of hypertension, systolic blood pressure measured in conscious state was significantly higher in 2K1C (216⁑18 mmHg) and DOCA-salt (205⁑29 mmHg) groups than that in control (128⁑4 mmHg). The third branches (<300 μm in outer diameter) of the mesenteric artery were isolated and cut into ring segments of 2∼3 mm in length. Each ring segment was mounted in tissue bath and connected to a force displacement transducer for measurement of isometric tension. The arterial rings were contracted by application of norepinephrine (NE) in a dose-dependent manner. The amplitude of the NE-induced contraction of the vessels was significantly larger in hypertension than in control. The NE-induced contraction was significantly enhanced by neuropeptide Y (NPY) in hypertension. Reciprocally, NPY-elicited vasocontraction was increased by NE in hypertension. These results suggest that the sympathetic nervous system contributes to the development of 2K1C and DOCA-salt hypertension.
고성능 액체크로마토그래피를 이용한 식이보충제 달맞이꽃종자 추출물의 펜타갈로일-β-D-글루코스의 검증된 분석법 개발과 응용
박상욱(Sang-Wook Park),김광준(Kwang Joon Kim),이원재(Wonjae Lee) 한국생물공학회 2020 KSBB Journal Vol.35 No.2
The purpose of this study is to develop and apply for determination of penta-O-galloyl-β-D-glucose (PGG), an essential marker compound in extract of evening-primrose seeds used as dietary supplement using by high-performance liquid chromatography (HPLC). Liquid chromatography was performed on a Capcellpak UG 120 column (4.6 ㎜ ID × 250 ㎜, 5 ㎛) using the gradient elution system developed in this study. According to the guidelines of the Association of Official Analytical Chemists for dietary supplements, the performance characteristics of HPLC were evaluated in terms of selectivity, linearity, precision, accuracy, recovery, limit of detection and limit of quantification. The calibration curve was highly linear (the coefficient of determination: 0.9999) within the concentration range of 0.42~26.88 ㎍/mL for PGG. The limit of detection and limit of quantitation of PGG in this method were 0.04 ㎍/mL and 0.13 ㎍/mL, respectively. This analytical method for determination of PGG as the marker compound was applied for domestic commercially available three extracts of evening-primrose seeds used as dietary supplements. The recoveries for these three fortified concentrations were 96.7~100.6%, 96.5~98.3% and 100.6~104.2%, respectively. It is expected that the validated HPLC analytical method developed in this study will be usefully applicable to determination of PGG in extract of evening-primrose seeds as dietary supplement for its quality control.