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JongUn Lee,Sungsu Lee,Sunmi Kim,Won Jung Lee 대한생리학회-대한약리학회 2002 The Korean Journal of Physiology & Pharmacology Vol.6 No.2
<P> The pathophysiological implications of aldosterone and adrenomedullin in the cardiac ventricular hypertrophy were examined. Male Sprague-Dawley rats were treated with deoxycorticosterone acetate (DOCA)-salt and monocrotaline (MCT) to selectively elicit left and right ventricular (LV, RV) hypertrophy, respectively. The mRNA expression of aldosterone synthase and adrenomedullin in LV and RV was determined by reverse transcription-polymerase chain reaction. The expression of aldosterone synthase and adrenomedullin was increased in LV, while not altered significantly in RV of DOCA-salt-treated rats. On the contrary, the expression was not significantly altered in LV, but increased in RV of MCT-treated rats. The enhanced expression of aldosterone synthase may be causally related with the development of ventricular hypertrophy, and the increased expression of adrenomedullin may act as a counter-regulatory mechanism.
Diminished Vascular Guanylyl Cyclase Activity in Deoxycorticos- terone Acetate-Salt Hypertension
JongUn Lee,Jung Hee Hong 대한생리학회-대한약리학회 2000 The Korean Journal of Physiology & Pharmacology Vol.4 No.5
<P> Pathophysiological implications of the vascular nitric oxide (NO)/cGMP pathway in hypertension were investigated. Sprague-Dawley rats were made deoxycorticosterone acetate (DOCA)-salt hypertensive for six weeks. The protein expression of endothelial constitutive NO synthase (ecNOS) and the tissue content of NO were determined in the thoracic aorta. The protein expression and catalytic activity of soluble guanylyl cyclase (GC) were also determined. Systolic blood pressure measured on the day of experiment was significantly higher in the experimental group than in the control. The hypertension was associated with decreases in the vascular tissue content of NO metabolites, concomitantly with the expression of ecNOS proteins. The protein expression of GC was not affected, while its catalytic activity was significantly decreased in hypertension. These results indicate that the high blood pressure is associated with a decreased activity of vascular NO/cGMP pathway in DOCA-salt hypertension.
Decreased Expression of Aquaporin-2 Water Channels in the Kidney in Rats Treated with Reserpine
JongUn Lee,YoonWha Oh,Sunmi Kim,Soo Wan Kim 대한생리학회-대한약리학회 2001 The Korean Journal of Physiology & Pharmacology Vol.5 No.4
<P> Whether there exists a sympathetic neural mechanism regulating the expression of aquaporin (AQP) water channels in the kidney was investigated. Male Sprague-Dawley rats were treated with reserpine (1 mg/kg, <I>IP</I>), and the expression of AQP1-4 proteins was determined in the kidney one day thereafter. Following the treatment with reserpine, the systolic blood pressure measured in a conscious state was significantly decreased in the experimental group compared with that in the control (83⁑8 vs 124⁑6 mmHg; n=6 each, P<0.05). The expression of AQP2 proteins was decreased in the cortex, outer medulla, and inner medulla. The decrease of AQP2 proteins was in parallel in the membrane and the cytoplasmic fractions, suggesting a preserved AQP2 targeting. No significant changes were observed in the expression of AQP1, AQP3, or AQP4. Neither basal nor AVP-stimulated formation of cAMP was significantly altered. These results suggest that the sympathetic nervous system has a tonic stimulatory effect specifically on the expression of AQP2 water channels in the kidney.
Decreased Expression of Aquaporin Water Channels in Denervated Rat Kidney
Lee, JongUn,Yoo, Kwangjay,Kim, Soo Wan,Jung, Kyoon Ho,Ma, Seong Kwon,Lee, Yeon Kyung,Kim, Wan-Young,Kim, Jin,Choi, Ki Chul S. Karger AG 2006 Nephron Vol.103 No.4
<P><I>Aims:</I> A neural mechanism regulating aquaporin (AQP) water channels in the kidney was investigated. <I>Methods:</I> Male Sprague-Dawley rats were used. Renal denervation was induced by painting the renal vessels with 10% phenol. The expression of AQP1-4 proteins was determined in the denervated and contralateral kidneys. The expression was also examined in rats which were renally denervated and subjected to water restriction or deoxycorticosterone acetate (DOCA)-salt treatment. <I>Results:</I> Following the unilateral denervation, tissue contents of norepinephrine were significantly decreased in the denervated kidney, while increased in the contralateral kidney. Accordingly, the expression of AQP1-4 proteins was decreased by 15-40% in the denervated kidney, and increased by 30-50% in the contralateral kidney. Immunohistochemistry of AQP2 confirmed its decreases in the denervated kidney and increases in the contralateral kidney. In bilaterally denervated rats, the urine flow increased along with decreased osmolarity. The water restriction increased the expression of AQP channels, however, the magnitude of which was lower in the denervated than in the contralateral kidney. Renal denervation decreased the degree of DOCA-salt hypertension, along with lower expression of AQP channels. <I>Conclusion:</I> It is suggested that the sympathetic nerve should play a specific excitatory role in the regulation of AQP channels in the kidney.</P><P>Copyright © 2006 S. Karger AG, Basel</P>
Sympathetic Regulation of Aquaporin Water Channels in Rat Kidney
JongUn Lee,Kwangjay Yoo,YoonWha Oh,Dong Yoon Lim 대한생리학회-대한약리학회 2003 The Korean Journal of Physiology & Pharmacology Vol.5 No.1
Whether there exists a sympathetic neural regulation on the aquaporin (AQP) channels in the kidney was examined. Male Sprague-Dawley rats were used. They were renal nerve denervated by stripping the nervous and connective tissues passing along the renal artery and vein, and painting these vessels with 10% phenol solution through a midline abdominal incision. Three days later, the expression of AQP1-4 proteins in the denervated kidneys was determined. The content of norepinephrine was found significantly decreased following the denervation. Accordingly, the expression of AQP2 proteins was markedly decreased. The expression of AQP3 and AQP4 was also slightly but significantly decreased, while that of AQP1 was not. Neither the basal nor the AVP-stimulated accumulation of cAMP was significantly affected in the denervated kidney. It is suggested that the sympathetic nervous system has a tonic stimulatory effect on AQP channels in the kidney.
Increased Expression of Nitric Oxide Synthases in Left and Right Ventricular Hypertrophy
JongUn Lee,Yoon Wha Oh,Sunmi Kim,Dae Gill Kang,Won Jung Lee 대한생리학회-대한약리학회 2001 The Korean Journal of Physiology & Pharmacology Vol.5 No.1
<P> The present study was aimed to explore pathophysiological implications of nitric oxide in the development of left and right ventricular hypertrophy. To induce selective left and right ventricular hypertrophy, rats were made two-kidney, one clip (2K1C) hypertensive and treated with monocrotaline (MCT), respectively. Six weeks later, the hearts were taken and their ventricular tissue mRNA and protein expression of endothelial constitutive isoform of nitric oxide synthase (NOS) were determined by reverse transcription-polymerase chain reaction and Western blot analysis, respectively. In 2K1C hypertensive rats, the expression of NOS mRNA was increased in parallel with its proteins in the left ventricle, but not in the right ventricle. In MCT-treated rats, the expression of NOS mRNA and proteins were proportionally increased in the right ventricle, but not in the left ventricle. These results suggest that the expression of NOS is specifically increased in association with the ventricular hypertrophy, which may be a mechanism counteracting the hypertrophy.
도시 직장인의 자기효능감이 조직몰입에 미치는 영향: 자아탄력성의 매개효과
이종운 ( Jongun Lee ),허만세 ( Manse Heo ) 사단법인 아시아문화학술원 2021 인문사회 21 Vol.12 No.4
본 연구는 도시 직장인의 자기효능감이 자아탄력성과 조직몰입에 미치는 영향을 살펴보고 자아탄력성의 매개효과를 검증하고자 하였다. 이를 위해 D지역에 거주하는 도시 직장인 274명을 대상으로 자기기입식 설문지조사를 통해 자료를 수집하여 자료분석에 활용하였다. 자료분석은 기술통계, 상관관계 분석 및 회귀분석 그리고 자아탄력성의 매개효과 분석을 실시하였다. 본 연구결과, 도시 직장인의 자기효능감은 조직몰입의 하위요인 정서적 몰입, 지속적 몰입에 매우 유의미하게 영향을 주는 것으로 나타났다. 또한 자아탄력성이 도시 직장인의 자기효능감과 조직몰입 간 유의하게 매개하고 있다는 것을 확인하였다. 따라서 도시 직장인들이 자기효능감 형성을 통해 자아탄력성의 심리적 자본을 형성할 수 있도록 지지하고 조직몰입에 집중할 수 있도록 지원방안을 마련되어야 할 것이다. This study sought to examine the effect of self-elasticity and tissue immersion of urban office workers and to verify the mediated effect of self-elasticity. To this end, 274 city office workers living in D area were collected through self-published questionnaires and used for data analysis. Data analysis performed descriptive statistics, correlation and regression, and the mediated effects of self-elasticity. As a result of this study, the sense of self-efficacy of urban office workers has a very significant impact on emotional immersion and continuous immersion, which are sub-factors of organizational immersion. It was also confirmed that self-elasticity significantly mediates between self-efficacy and organizational immersion of urban office workers. Therefore, it should support city workers to form psychological capital of self-elasticity through self-efficacy and provide support measures to focus on organizational immersion.