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Effects of Additives on Nutrients and Fermentation Quality of Alfalfa Silage
Xiaoqing Zhang,Qizhong Sun,Xingyou Li,Xiaoxia Li 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
Two-year-old, full flower aohan alfalfa (Medicaga sativa cv. aohan) was chosen to evaluate the silage effect by using different additives of Qingbao Ⅱ , Caihe and Yishengkang. Result indicated that additives increased CP content (P<0.05) and decreased CF, ADF and NDF content significantly (P<0.05). Lactic acid content in additives treatments were significantly higher (P<0.01) than control. These result showed that additives improved silage fermentation quality, reduced cellulose contents and improved silage nutritive value. Among three treatments, Caihe and Yishengkang were priority to Qingbao Ⅱ on the effect of promoting the desired lactic acid fermentation.
Xiaoqing Li,Yinghui Kong,He Li,Manyuan Xu,Ming Jiang,Weiguo Sun,Suping Xu 한국유전학회 2021 Genes & Genomics Vol.43 No.5
Background Cutaneous squamous cell carcinoma (CSCC) is a severe malignancy derived from skin. Dysregulated circular RNAs (circRNAs) might play vital roles in tumor development. Objective Here, we aimed to explore the function of a novel circRNA circ_0067772 in CSCC. Methods Quantitative real-time PCR (qRT-PCR) or Western blot assay was performed to determine the expression of circ_0067772, microRNA (miR)-1238-3p and forkhead box protein G1 (FOXG1). Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Transwell assay and wound healing assay were employed to examine cell metastasis. Flow cytometry was employed to monitor cell cycle and apoptosis. The target association between miR-1238-3p and circ_0067772 or FOXG1 was validated by dual-luciferase reporter assay. Moreover, role of circ_0067772 in vivo was investigated via xenograft model in nude mice. Results Circ_0067772 and FOXG1 were upregulated, while miR-1238-3p was downregulated in CSCC tissues and cells. Circ_0067772 knockdown conferred inhibitory efects on cell proliferation, migration and invasion of CSCC cells. MiR1238-3p served as a target of circ_0067772, whose silencing could reverse circ_0067772 knockdown-induced inhibitory impact on the malignant cellular behaviors. Circ_0067772 positively regulated FOXG1 expression by antagonizing miR1238-3p. Additionally, miR-1238-3p could repress CSCC cell proliferation, migration and invasion by targeting FOXG1. Also, circ_0067772 knockdown hindered CSCC tumor growth in vivo. Conclusion Our study identifed a novel oncogenic circRNA and the involvement of circ_0067772/miR-1238-3p/FOXG1 axis in CSCC development, providing a target for CSCC therapy.
Sun, Wei,Li, Xiaoqing,Liu, Shufeng,Jiao, Kui Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.3
Direct electron transfer of hemoglobin (Hb) in the chitosan (CTS) and $TiO_2$ nanoparticles (nano-$TiO_2$) composite films was achieved by using a room temperature ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate ($BMIMPF_6$) modified carbon paste electrode (CILE) as the basal electrode. UV-Vis and FT-IR spectroscopy indicated that Hb in the film retained the native structure. Electrochemical investigation indicated that a pair of well-defined quasi-reversible redox peaks of Hb heme Fe(III)/Fe(II) was obtained with the formal potential located at -0.340 V (νs. SCE) in pH 7.0 phosphate buffer solution (PBS). The electrochemical parameters such as the electron transfer coefficient (α), the electron transfer number (n) and the standard electron transfer rate constant ($k_s$) were got as 0.422, 0.93 and 0.117 $s^{-1}$, respectively. The fabricated CTS/nano-$TiO_2$/Hb/CILE showed good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) and hydrogen peroxide ($H_2O_2$), which exhibited a potential application in fabricating a new kind of third generation biosensor.
Han, Xiaoqing,Luan, Tao,Sun, Yingying,Yan, Wenyi,Wang, Dake,Zeng, Xianlu Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.9
Myeloid-derived suppressor cells (MDSCs) promote tumour progression by contributing to angiogenesis, immunosuppression, and immunotherapy resistance. Although recent studies have shown that microRNAs (miRNAs) can promote the expansion of MDSCs in the tumour environment, the mechanisms involved in this process are largely unknown. Here, we report that microRNA 449c (miR-449c) expression was upregulated in myeloid progenitor cells upon activation of C-X-C motif chemokine receptor 2 (CXCR2) under tumour conditions. MiR-449c upregulation increased the generation of monocytic MDSCs (mo-MDSCs). The increased expression of miR-449c could target STAT6 mRNA in myeloid progenitor cells to shift the differentiation balance of myeloid progenitor cells and lead to an enhancement of the mo-MDSCs population in the tumour environment. Thus, our results demonstrate that the miR-449c/STAT6 axis is involved in the expansion of mo-MDSCs from myeloid progenitor cells upon activation of CXCR2, and thus, inhibition of miR-449c/STAT6 signalling may help to attenuate tumour progression.
Wei Sun,Xiaoqing Li,Shufeng Liu,Kui Jiao 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.3
Direct electron transfer of hemoglobin (Hb) in the chitosan (CTS) and TiO2 nanoparticles (nano-TiO2) composite films was achieved by using a room temperature ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6) modified carbon paste electrode (CILE) as the basal electrode. UV-Vis and FT-IR spectroscopy indicated that Hb in the film retained the native structure. Electrochemical investigation indicated that a pair of well-defined quasi-reversible redox peaks of Hb heme Fe(III)/Fe(II) was obtained with the formal potential located at -0.340 V (νs. SCE) in pH 7.0 phosphate buffer solution (PBS). The electrochemical parameters such as the electron transfer coefficient (α), the electron transfer number (n) and the standard electron transfer rate constant (ks) were got as 0.422, 0.93 and 0.117 s-1, respectively. The fabricated CTS/nano-TiO2/Hb/CILE showed good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) and hydrogen peroxide (H2O2), which exhibited a potential application in fabricating a new kind of third generation biosensor.
Hu Hongjun,Sun Zhao,Ou zhongwen,Wang xiaoqing 대한금속·재료학회 2017 METALS AND MATERIALS International Vol.23 No.3
A new severe plastic deformation method called extrusion-shearing shorten for “ES” has been developed to fabricatethe ultra-fine grained AZ61 magnesium alloys. The correlation theories of ES process have been studiedwhich includes cumulative strain and Zener-Hollomon parameter etc. Simulations of ES process for wroughtAZ61 magnesium alloy have been performed using three-dimensional finite element method. ES dies with onestep shearing and two step shearings have been designed, manufactured and installed onto thermo-mechanicalsimulator and industrial horizontal extruder, respectively. Microstructures evolution has been observed andanalysed. The influences of the ES processes on the grain refinements of AZ61magniesium alloys during multistageprocesses have been investigated. Based on the experimental, simulation and theoretical results, ES processcould increase the cumulative strains enormously and refine grain sizes by direct extrusion and additionalshearings. ES process can produce the serve plastic deformation and improve the volume fraction of dynamicrecrystallization. Continuous dynamic recrystallizaion is the main reason for grain refinements during ES process.
Huie Chen,Haotian Guo,Xiaoqing Yuan,Yating Chen,Chao Sun 대한토목학회 2020 KSCE Journal of Civil Engineering Vol.24 No.9
The strength characteristics of unsaturated silty clay after the cooling and single freeze-thawcycle were studied by using Global Digital Systems Ltd. (GDS) triaxial test system aftercontrolling initial matric suction. The results demonstrate that, the initial matrix suction andfreezing process can strengthen the shear strength of the soil, which makes the stress-straincurve of the soil show certain strain hardening characteristics at different temperatures. whenthe temperature is negative (including 0°C), the curve has a flat transition section whosestrength does not change with deformation, and the flat transition section becomes shorter asthe temperature and confining pressure decrease. The strength of the soil at negativetemperature and the speed at which it reaches its peak value are much greater than those ofthe soil after positive temperature and single freeze-thaw cycle, and the contribution ofconfining pressure to the strength decreases with the decrease of temperature. The effect oftemperature change (15oC to -15oC) on soil strength is mainly reflected in the total cohesion,and the effect of freeze-thaw on the effective internal friction angle is more significant. Thestrength and deformation characteristics of soil under the freezing process and freeze-thawcycle are determined by the initial matric suction, the form of connection between soilparticles, the migration of unfrozen water, and the degree of water-ice phase transformation.
Wang, Xinglong,Liu, Li,Liu, Sixiu,Sun, Xiaoqing,Deng, Zhongxiang,Pi, Yan,Sun, Xiaofen,Tang, Kexuan Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.5
A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa-pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.