Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography-electron spray ionization-MS/MS

Ting-Ting Sun,Xin-Lei Liang,He-Yun Zhu,Xu-Ling Peng,Xing-Jie Guo,Long-Shan Zhao 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.3

Background: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng. Methods: A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLCeMS/MS) method was developed to separate and identify saponins in Shizhu ginseng. Results: The separation was carried out on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 ㎛) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at 40℃. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances. Conclusion: The developed UHPLCeMS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng.

Long Non-coding RNAs are Differentially Expressed in Hepatocellular Carcinoma Cell Lines with Differing Metastatic Potential

Fang, Ting-Ting,Sun, Xiao-Jing,Chen, Jie,Zhao, Yan,Sun, Rui-Xia,Ren, Ning,Liu, Bin-Bin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: Metastasis is a major reason for poor prognosis in patients with cancer, including hepatocellular carcinoma (HCC). A salient feature is the ability of cancer cells to colonize different organs. Long non-coding RNAs (lncRNAs) play important roles in numerous cellular processes, including metastasis. Materials and Methods: In this study, the lncRNA expression profiles of two HCC cell lines, one with high potential for metastasis to the lung (HCCLM3) and the other to lymph nodes (HCCLYM-H2) were assessed using the Arraystar Human LncRNA Array v2.0, which contains 33,045 lncRNAs and 30,215 mRNAs. Coding-non-coding gene co-expression (CNC) networks were constructed and gene set enrichment analysis (GSEA) was performed to identify lncRNAs with potential functions in organ-specific metastasis. Levels of two representative lncRNAs and one representative mRNA, RP5-1014O16.1, lincRNA-TSPAN8 and TSPAN8, were further detected in HCC cell lines with differing metastasis potential by qRT-PCR. Results: Using microarray data, we identified 1,482 lncRNAs and 1,629 mRNAs that were differentially expressed (${\geq}1.5$ fold-change) between the two HCC cell lines. The most upregulated lncRNAs in H2 were RP11-672F9.1, RP5-1014O16.1, and RP11-501G6.1, while the most downregulated ones were lincRNA-TSPAN8, lincRNA-CALCA, C14orf132, NCRNA00173, and CR613944. The most upregulated mRNAs in H2 were C15orf48, PSG2, and PSG8, while the most downregulated ones were CALCB, CD81, CD24, TSPAN8, and SOST. Among them, lincRNA-TSPAN8 and TSPAN8 were found highly expressed in high lung metastatic potential HCC cells, while lowly expressed in no or low lung metastatic potential HCC cells. RP5-1014O16.1 was highly expressed in high lymphatic metastatic potential HCC cell lines, while lowly expressed in no lymphatic metastatic potential HCC cell lines. Conclusions: We provide the first detailed description of lncRNA expression profiles related to organ-specific metastasis in HCC. We demonstrated that a large number of lncRNAs may play important roles in driving HCC cells to metastasize to different sites; these lncRNAs may provide novel molecular biomarkers and offer a new basis for combating metastasis in HCC cases.

Image Retrieval Based on Deep Belief Networks

Sun Ting,Qi Yingchun 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.1

According to the local and global feature of image, matching the image from a lot image library, this is the image retrieval task; however, the image retrieval need to search the information in the database, we need to find a method for efficient information retrieval. Deep belief network according to the characteristic of the initiative, through the method of training a multilayer neural network to process large amounts of data, and it is very efficient, in this article, as to the characteristics of image local features and global features, it gives a deep belief network image retrieval algorithm, the experiment verify the effectiveness of the algorithm.

Fabrication of polyacrylic acid-based composite binders with strong binding forces on copper foils for silicon anodes in lithium-ion batteries

Ting-Ting Su,Wen-Feng Ren,Jiang-Meng Yuan,Ke Wang,Bing-Yu Chi,Run-Cang Sun 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

The commercial application of silicon (Si) anodes with high theoretical capacity is hampered by the poorcyclic stability because the huge volume change of Si during discharge/charge processes results in thepulverization of electrode materials and the electric contact loss of electrode materials with copper(Cu) foils. Binders play an important role for adhering active materials and conductive additives togetheronto Cu foils. Herein, we design and develop a three-dimensional networked composite binder (PAA-co-SN) via an amidation reaction between polyacrylic acid (PAA) and thiourea (SN). The granular anchors(CuxS) are constructed at the interface between PAA-co-SN binders and Cu foils, resulting from the reactionof S and Cu during the drying processes of Si electrodes. When used as binders for Si nanoparticles,they exhibit stable cyclic performance (1580 mAh g1 after 500 cycles). The anchoring mechanismbetween composite binders and Cu foils provides a strategy for the improvement of electrochemicalproperties of Si-based anodes for lithium-ion batteries.

Image Retrieval Method for Deep Neural Network

Sun Ting,Geng Guohua 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.7

Because of the large data in the image database, the key problem of the retrieval algorithm is to retrieve the required image in the short time. Aiming at this problem, this article given a self-learning deep belief neural network method, and through building layers, input, output, and self-learning algorithm in network architecture to get global algorithm for image retrieval. The accuracy and the convergence of the proposed retrieval method are verified by experiments.

A Curve-Skeleton Extraction Algorithm based on Vector Fields for 3D Model Retrieval

Sun Ting,Zhou Wen-Gang,Geng Guo-Huo 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.9

Feature extraction is a key issue for 3d model retrieval. A novel architecture to extract the curve-skeleton of 3d model is introduced. The algorithm firstly calculates the 3D vector fields of models represented by discrete unit, and then extracts the hierarchical curve-skeleton based on topological features of the critical curve and critical points of the vector fields. The similarity among 3D curve-skeletons is measured by using an improved Earth Mover's Distance (EMD) algorithm. The curve-skeleton extracted with this novel algorithm can be used to categorize models and implement global matching and partial matching.

Preparation of biomass-based yam solar absorber for enhanced solar evaporation application

Ting Li,Juan Jia,Yanqing Wang,Hanxue Sun,Jiyan Li,Zhaoqi Zhu,Weidong Liang 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.11

The solar evaporation system has become a research hotspot in the field of photothermal conversion technology in recent years because of its high photothermal conversion efficiency and practicality of promotion. Biomass solar absorbers have excellent solar absorption properties and high solar-water vapor conversion, but they have the limitations of long feedstock production cycle and high carbonization temperature. As a kind of biomass materials, yam is not only inexpensive, but also has a short production cycle and rich pore structures. Therefore, in this paper, a yam based solar absorber with rich pore structure was prepared, and the photothermal conversion efficiency of the absorber was further investigated. The yam was treated with freeze-dried method and carbonized at different temperatures to obtain yam solar absorbers. Then the thermal stability and porosity of solar absorber was found to gradually rise as the treatment temperature increased. The solar absorption rate of the carbonized yam was close to 90% in the near UV-visible region. Solar-water vapor control experiments with different treatments of the solar absorber of the yam were carried out in simulated solar-driven interface steam generation system (SISGS). An absorber treated at 200 °C in the sun was found to have the best performance with a solar-water vapor conversion of about 90% and a water evaporation rate of 1.3164 kg m−2 h−1.

Preparation and Mechanical Property Evaluations of Puncture-Resistant Insoles Composites Reinforced by High-Modulus Filament and Thermal Bonding

Ting-Ting Li,Fei Sun,Xing Liu,Hua-Ling Wu,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2018 Fibers and polymers Vol.19 No.6

High-modulus PET filaments and thermal bonding are used to reinforce the puncture resistance stability of the insole composite. This study aims to discuss the influences of the amount of low-melting-point polyester fibers (LMPET) and needle densities (ND) on tensile, bursting, and quasi-static puncture resistance properties. Besides, significance of LMPET amount and ND on puncture resistance against flat-head (A), spherical-head (B), and pointed-head (C) probes are in particular investigated to simulate the diversified application environments for insoles. Research result shows that, LMPET amount significantly affects the static puncture resistance against three probes; ND only significantly influences the puncture resistance against Probe A and C. Thermal bonding significantly improves the puncture strength against Probe B with various LMPET amounts of insoles, but evidently increases the puncture resistance against Probe A and C when being punched at various ND. The amount of LMPET fibers has a positive influence on the puncture strength of insoles, and 70 wt% of LMPET provide the average static puncture resistance up to 342.6 N. The high-modulus resultant insoles have advantages of flexibility, ease of process, and bendability with a higher and more stabilized puncture resistances.

Polyvinylidene Fluoride Electrospun Fibers Loaded TiO2 for Photocatalytic Degradation and Oil/Water Separation

Fei Sun,Hai-Tao Ren,Shih-Yu Huang,Ting-Ting Li,Hao-Kai Peng,Qi Lin,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2020 Fibers and polymers Vol.21 No.7

In this study, electrospun polyvinylidene fluoride (PVDF)-based nanofibrous membranes embedding TiO2 wereprepared and used for photocatalytic degradation and oil/water separation. The nanofibrous membranes were characterizedby scanning electron microscopy (SEM), transmission electronic microscope (TEM), X-ray diffraction (XRD), Fouriertransform infrared spectroscopy (FTIR) spectroscopy, thermal performance analysis, and oil/water separation analysis. Andthe degradation of rhodamine B dye was also investigated. Results showed that the fiber diameter and thermal stability of themembranes decreased with the increasing of TiO2. Meanwhile, the fiber surface roughness and specific surface areaincreased. The analysis of TEM, XRD and FTIR indicated that TiO2 existed in the PVDF membranes. When the TiO2 contentwas 12 %, the fiber diameter of the membranes was about 110 nm, and the photocatalytic degradation of rhodamine B dyeefficiency was up to 97 %. The reaction rate constant was 0.02057 min-1. At 0.01 MPa vacuum, oil separated from watereffectively, which proves that separation can be easily conducted with a low energy cost. Thus, the prepared membranes havea very high application prospect in the purification of reclaimed water and separation of oil and water.

중국 백화점의 물리적 환경에 따른 소비자의 내적반응이 점포충성도에 미치는 영향

장정정 ( Ting Ting Zhang ),전지현 ( Ji Hyun Jun ),이영선 ( Young Sun Rhee ) 한국의류학회 2013 한국의류학회지 Vol.37 No.2

Outstanding service quality is a crucial factor to increase customer revisits to increase customer revisits to create new customers through positive word-of-mouth marketing and increase profits; in conjunction the physical environment of department stores have a firsthand impact on customer purchasing behavior. This study examines the physical environment dimensions of department stores in China and the causal re-lationship with the physical environment service environment internal responses and store loyalty. The subjects in this study were male and female adults who finished the shopping the Lotte and Wangfujing Department Store located in Beijing. All participants were over the age of 20 and a one-on-one survey was conducted to gather data from October to November 2011. Data from 424 respondents were analyzed: in addition AMOS factor analysis and path analysis were used. The results were as follows. First physical environment factors consist of exterior aesthetic attraction convenience cleanliness and an interior aesthetic attraction. Second exterior aesthetic attraction convenience and cleanliness affect the overall service envir-onment perception. Third the service environment exercised an influence on internal responses and internal impacted store loyalty. Fourth a comparative analysis was made over korean and Chinese de-partment stores in China to check the research model. In Wangfujing Department Store exterior aesthetic attraction cleanliness and convenience had a statistically significant impact on service environment percep-tions. In Lotte Department Store the perception of service environment was under the statistically significant influence of all physical factors (exterior aesthetic attraction interior aesthetic attraction convenience and cleanliness). The findings of this study are helpful for Korean Department stores that plan to make inroads into China to formulate efficient service environment strategies tailored to local consumer characteristics.