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Sudhir Kumar,Neha Saxena,Ajay Mandal 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.43 No.-
In this study an attention has been paid to synthesize polymer grafted anionic surfactant (PMES) derivedfrom nonedible vegetable oil (Jatropha) for its application in enhanced oil recovery (EOR). The polymericsurfactant was prepared by reacting acrylamide monomer with methyl ester sulfonate (MES) synthesizedfrom Jatropha oil by free radical polymerization mechanism. Polymeric surfactant with properties of bothsurfactant and polymer can control the mobility ratio and reduce interfacial tension (IFT), which aredesirable for EOR. The synthesized polymeric surfactant was characterized through FTIR, 1H NMR, FESEM,EDX, TGA, DLS analysis. The effectiveness of PMES for chemically enhanced oil recovery process wasinvestigated by measurement of physiochemical properties of its aqueous solution viz. reduction of IFT,wettability alteration and rheological behaviour. Rheological studies shows shear thinning behaviourwith apparent viscosity comparable to conventional polymers. The IFT between crude oil and aqueousPMES solution at its critical micelle concentration (CMC) was observed as 2.74 mN/m, which was furtherreduced to 0.37 mN/m on addition of 2.5 wt% NaCl. Coreflooding experiments were conducted insandpack system, to study the EOR efficiency using the synthesized polymeric surfactant and more than26% additional recovery was observed after usual waterflooding. Higher recoveries at highertemperature were observed because of swelling of crude oil and lowering of IFT.
Sudhir Kumar Srivastava,Rishi Anil Aggarwal,Sunil Krishna Bhosale,Kunal Roy,Pradip Sharad Nemade 대한척추외과학회 2017 Asian Spine Journal Vol.11 No.2
Study Design: Retrospective case series. Purpose: To describe a novel single incision that combines anterior and posterior approaches for decompression and instrumented fusion to treat tuberculosis of the thoracic spine and study the neurological and radiological outcomes. Overview of Literature: Tuberculosis of the spine remains a major health issue in many developing countries. The options for treating tuberculosis of the thoracic spine include the anterior, posterior, and combined approaches, each with its advantages and disadvantages. Methods: Totally, 143 patients with tuberculosis of the thoracic spine were surgically treated using the “Versatile approach”. Posterior fixation was performed using sublaminar wires and a Hartshill rectangle in all patients. Anterior reconstruction was accomplished using bone graft harvested from autologous rib, iliac crest, or fibula. Results: The study included 45 males and 98 females, with a mean age of 33.18±18.65 years (range, 3–82 years) and a mean followup of 60.23±24.56 months (range, 18–156 months). Kyphosis improved from a mean value of 24.02 preoperatively to 10.25 postoperatively. A preoperative neurological deficit was observed in 131 patients, with 130 patients regaining ambulatory power. No patient had deterioration of neurological status following surgery. Fusion was achieved in all cases. The visual analogscale score improved from an average score of 7.02 preoperatively to 1.51 at final follow-up. Eight patients had superficial macerations, which healed spontaneously. One patient had buckling of the anterior graft, and one patient had implant breakage following road traffic accident. Conclusions: The “Versatile approach” is an effective, single-stage, single-incision method that combines anterior and posterior approaches for the surgically treating tuberculosis of the thoracic spine. It offers the advantage of direct visualization for decompression and reconstruction of the anterior and posterior vertebral columns, thus providing an excellent, long-lasting clinical outcome.
Sudhir Kumar Srivastava,Rishi Anil Aggarwal,Sunil Krishna Bhosale,Kunal Roy,Pradip Sharad Nemade,Shaligram Purohit 대한척추외과학회 2019 Asian Spine Journal Vol.13 No.3
Study Design: Retrospective case series. Purpose: Describe the technique and evaluate the outcome of apical vertebral column resection (VCR) with sagittal rotation and anterior opening and posterior closing (AOPC) maneuver for correction of severe post-tubercular kyphosis (PTK). Overview of Literature: The surgical procedures described for the correction of PTK are VCR, pedicle subtraction osteotomy, transpedicular decancellation osteotomy, and closing-opening osteotomy. Methods: We retrospectively evaluated 21 patients who had been operated on with single stage apical VCR with AOPC maneuver. Radiographs were obtained before surgery and at regular follow-up intervals. These were used to calculate the angle of kyphosis. Back pain was rated using the Visual Analog Scale (VAS) and neurological status was graded using Frankel grading. Radiological outcome was assessed by the improvement in the angle of kyphosis and fusion following surgery. Neurological status was assessed using Frankel grading. Results: The study included eight males and 13 females with a mean age of 21.9 and average follow-up time of 30.4 months. The average number of vertebral bodies destroyed was 2.57. Kyphosis was improved from a mean of 68.42°±13.23° preoperative to 8.71°±4.58° postoperative. The average correction achieved was 87.10%. Preoperative VAS score improved from a mean of 6.38±0.92 preoperative to 1.38±0.49 postoperative. No patients had any sign of neurological deterioration. Seven out of eight patients with a preoperative neurological deficit improved following surgery. Two patients developed superficial wound maceration, one had persistent postoperative hypotension, and the other developed hemothorax. All patients recovered fully without a need for additional surgery. Conclusions: Single stage simultaneous anterior column lengthening and posterior column shortening is an effective method for surgical correction of severe PTK.
Sudhir Kumar Srivastava,Pradip Sharad Nemade,Rishi Anil Aggarwal,Sunil Krishna Bhoale 대한척추외과학회 2016 Asian Spine Journal Vol.10 No.1
Developmental anomalies of the axis are commonly encountered, especially anomalies involving the odontoid process. Anomalies of the posterior elements are uncommon. We describe a unique case of agenesis of posterior elements of C2 with basilar invagination and atlanto-axial dislocation. An obese 8-year-old boy presented with symptoms of cervical myelopathy. Radiological workup revealed a craniovertebral junction anomaly with occipitalised atlas, absent posterior elements of axis, and hypertrophied C3 spinous process. Atlanto-axial instability and basilar invagination was present. Magnetic resonance angiography revealed hypoplastic left vertebral artery. Traction with cervical tongs failed to improve the alignment and symptoms. Anterior trans-oral release, followed by posterior decompression and custom-made instrumentation, was done. The patient recovered completely and was asymptomatic at the end of two years. X-ray and computed tomography scan demonstrated reduction of basilar invagination and maintenance of alignment. This is the first case to be reported of agenesis of posterior elements of axis associated with basilar invagination. One should look for this condition in patients with hypertrophied spinous process of C3. Utilization of hypoplastic pedicle of axis serves as an additional fixation point to increase the stability of the construct.
Sudhir Navathe,Sakshi Singh,Vinay Kumar Singh,Ramesh Chand,Vinod Kumar Mishra,Arun Kumar Joshi 한국유전학회 2019 Genes & Genomics Vol.41 No.9
Background Membrane-bound NADPH oxidases (Nicotinamide adenine ainucleotide phosphate oxidase) also called respiratory burst oxidase homologs (Rboh) play an essential role in ROS production under normal as well as environmental stress conditions in plants. Objective To identify and study respiratory burst homologs (Rboh) from the wheat genome as well as characterize their role in various biological and molecular processes along with expression in response to biotic and abiotic stresses. Methods The Rboh homologs in the wheat genome were predicted based on data processing, alignment of sequences and phylogenetic analysis of sequences in numerous plant species and wheat. The conserved motifs were known followed by domain design study. The 3-D structure prediction and similarity modeling were administered for NADPH enzyme domain. Gene ontology and a functional study were done in addition to expression analysis of Triticum aestivum respiratory burst oxidase (TaRboh) gene family in response to biotic as well as abiotic stress. Results Phylogenetic analysis of Rboh gene family members among seven plant species including wheat, classified the family into four subfamilies. Rboh genes are mainly involved in various biological processes such as Response to oxidative stress, Superoxide anion generation, Hydrogen peroxide biosynthetic process. Among the molecular functions, calcium ion binding, peroxidase activity, oxidoreductase activity, superoxide-generating NADPH oxidase activity are essential. Enzyme annotation of the family and superfamily revealed that it encodes to five structural clusters and coding to enzymes NAD(P)H oxidase ( H2O2-forming) (EC:1.6.3.1), Ferric-chelate reductase (NADH) (EC: 1.16.1.7), Peroxidase (EC: 1.11.1.7), Ribose-phosphate diphosphokinase (EC: 2.7.6.1). The enzymes contain six membrane-spanning domains, two hemes, and conserved motifs associated with NADPH, EF-hand and FAD binding. The outcomes additionally reflect a distinct role of this enzyme in different molecular functions which are responsible for the stress signaling. Further, the transcripts of TaRboh found expressed in various plant parts such as stem, leaves, spike, seed, and roots. We also observed expression of these gene family members under drought/combination of drought + heat and important wheat pathogens such as Puccinia striformis, Blumeria graminis f.sp. tritici, Fusarium graminiarum, F. pseudograminiarum, and Zymoseptoria tritici. Conclusions The investigation demonstrated that identified respiratory burst homologs (Rboh) in T. aestivum were involved in pathogen activated ROS production and have regulatory functions in cell death and defense responses.
Kumar, Sudhir,Jagielski, Jakub,Kallikounis, Nikolaos,Kim, Young-Hoon,Wolf, Christoph,Jenny, Florian,Tian, Tian,Hofer, Corinne J.,Chiu, Yu-Cheng,Stark, Wendelin J.,Lee, Tae-Woo,Shih, Chih-Jen American Chemical Society 2017 NANO LETTERS Vol.17 No.9
<P>Pure green light-emitting diodes (LEDs) are essential for realizing an ultrawide color gamut in next-generation displays, as is defined by the recommendation (Rec.) 2020 standard. However, because the human eye is more sensitive to the green spectral region, it is not yet possible to achieve an ultrapure green electroluminescence (EL) with a sufficiently narrow bandwidth that covers >95% of the Rec. 2020 standard in the CIE 1931 color space. Here, we demonstrate efficient, ultrapure green EL based on the colloidal two-dimensional (2D) formamidinium lead bromide (FAPbBr(3)) hybrid perovskites. Through the dielectric quantum well (DQW) engineering, the quantum-confined 2D FAPbBr(3) perovskites exhibit a high exciton binding energy of 162 meV, resulting in a high photoluminescence quantum yield (PLQY) of similar to 92% in the spin-coated films. Our optimized LED devices show a maximum current efficiency (eta(CE)) of 13.02 cd A(-1) and the CIE 1931 color coordinates of (0.168, 0.773). The color gamut covers 97% and 99% of the Rec. 2020 standard in the CIE 1931 and the CIE 1976 color space, respectively, representing the 'greenest' LEDs ever reported. Moreover, the device shows only a similar to 10% roll-off in eta(CE) (11.3 cd A(-1)) at 1000 cd m(-2). We further demonstrate large-area (3 cm(2)) and ultraflexible (bending radius of 2 mm) LEDs based on 2D perovskites.</P>