效率的인 指導力 訓練 프로그램의 開發 : 大學生用

陳渭敎,尹正倫,金晟會,石秀隆,文昌鉉 경북대학교 학생생활연구소 1992 學生指導硏究 Vol.25 No.1

Chemical Modification of the Human Ether-a-go-go-related gene (HERG) K+ Current by the Amino-Group Reagent Trinitrobenzene Sulfonic Acid

Su-Hyun Jo,Se-Young Choi,Ji-Hyun Yun,Young-Sang Koh,Won-Kyung Ho,Chin O. Lee 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.4

We investigated the effects of trinitrobenzene sulfonic acid (TNBS), an amino-group reagent, on the human ether-a-go-go-related gene (HERG) K+ channels expressed in Xenopus oocytes. TNBS neutralizes the positively charged amino-groups of peptide N-terminal and lysine residues. External application of TNBS at 10 mM for 5 min irreversibly shifted the curves for currents at the end of the pulse and tail currents of HERG to a more negative potential and decreased the maximal amplitude of the Itail curve (Itail,max). TNBS had little effect on either the activated current-voltage relationship or the reversal potential of HERG current, indicating that TNBS did not change ion selectivity properties. TNBS shifted the time constant curves of both activation and deactivation of the HERG current to a more hyperpolarized potential; TNBS’s effect was greater on channel opening than channel closing. External H+ is known to inhibit HERG current by shifting V1/2 to the right and decreasing Itail,max. TNBS enhanced the blockade of external H+ by exaggerating the effect of H+ on Itail,max, not on V1/2. Our data provide evidence for the presence of essential amino-groups that are associated with the normal functioning of the HERG channel and evidence that these groups modify the blocking effect of external H+ on the current.

Chemical Modification of the Human Ether-a-go-go-related gene (HERG) $K^+$ Current by the Amino-Group Reagent Trinitrobenzene Sulfonic Acid

Jo Su-Hyun,Choi Se-Young,Yun Ji-Hyun,Koh Young-Sang,Ho Won-Kyung,Lee Chin-O. The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.4

We investigated the effects of trinitrobenzene sulfonic acid (TNBS), an amino-group reagent, on the human ether-a-go-go-related gene (HERG) $K^+$ channels expressed in Xenopus oocytes. TNBS neutralizes the positively charged amino-groups of peptide N-terminal and lysine residues. External application of TNBS at 10 mM for 5 min irreversibly shifted the curves for currents at the end of the pulse and tail currents of HERG to a more negative potential and decreased the maximal amplitude of the $I_{tail}$ curve $(I_{tail,max})$. TNBS had little effect on either the activated current-voltage relationship or the reversal potential of HERG current, indicating that TNBS did not change ion selectivity properties. TNBS shifted the time constant curves of both activation and deactivation of the HERG current to a more hyperpolarized potential; TNBS's effect was greater on channel opening than channel closing. External $H^+$ is known to inhibit HERG current by shifting $V_{1/2}$ to the right and decreasing $I_{tail,max}$. TNBS enhanced the blockade of external $H^+$ by exaggerating the effect of $H^+$ on $I_{tail,max}$, not on $V_{1/2}$. Our data provide evidence for the presence of essential amino-groups that are associated with the normal functioning of the HERG channel and evidence that these groups modify the blocking effect of external $H^+$ on the current.

전치부 개방교합을 동반한 골격성 제3급 부정교합 환자에 대한 양측 하악지 시상분할 골절단술후 안정성에 관한 임상적 분석

김현수(Hyun-Soo Kim),권대근(Tae-Geon Kwon),이상한(Sang-Han Lee),김진수(Chin-Su Kim),강동화(Dong-Hwa Kang),장현중(Hyun-Jung Jang) 대한구강악안면외과학회 2007 대한구강악안면외과학회지 Vol.33 No.2

This study was conducted to patients visited oral maxillo-facial surgery, KNUH and the purpose of the study was to assess skeletal and dento-alveolar stability after surgical-orthodontic correction treated by skeletal Class III malocclusion patients with open bite versus non-open bite. This retrospective study was based on the examination of 40 patient, 19 males and 21 females, with a mean age 22.3 years. The patients were divided into two groups based on open bite and non-open bite skeletal Class III malocclusion patients. The cephalometric records of 40 skeletal Class III malocclusion patients (open bite: n = 18, non-open bite: n = 22) were examined at different time point, i.e. before surgery(T1), immediately after surgery(T2), one year after surgery(T3). Bilateral sagittal split ramus osteotomy was performed in 40 patients. Rigid internal fixation was standard method used in all patient. Through analysis and evaluation of the cephalometric records, we were able to achieve following results of post-surgical stability and relapse. 1. There was no significant statistical differences between open bite and non-open bite with skeletal Class III malocclusion patients in maxillary occlusal plane angle of pre-operative stage(p>0.05). 2. Mean vertical relapses of skeletal Class III malocclusion patients with open bite were 0.02±1.43mm at B point and 0.42±1.56mm at Pogonion point. In skeletal Class III malocclusion patients with non-open bite, 0.12±1.55mm at B point and 0.08±1.57mm at Pogonion point. There was no significant statistical differences between open bite and non-open bite with skeletal Class III malocclusion patients in vertical relapse(p>0.05). 3. Mean horizontal relapses of skeletal Class III malocclusion patients with open bite were 1.22±2.21mm at B point and 0.74±2.25mm at Pogonion point. In skeletal Class III malocclusion patients with non-open bite, 0.92±1.81mm at B point and 0.83±2.11mm at Pogonion point. There was no significant statistical differences between open bite and non-open bite with skeletal Class III malocclusion patients in horizontal relapse(p>0.05). 4. There were no significant statistical differences between open bite and non-open bite with skeletal Class III malocclusion patients in post-surgical mandibular stability(p>0.05). and we believe this is due to minimized mandibular condylar positional change using mandibular condylar positioning system and also rigid fixation using miniplate 5. Although there was no significant relapse tendency observed at chin points, according to the Pearson correlation analysis, the mandibular relapse was influenced by the amount of vertical and horizontal movement of mandibular set-back(p=0.05, r>0.304).

인문계 고등학생의 학업성취도와 성격-기질과의 상관성에 관한 연구

진범수(Bum-Su Chin),윤승현(Seung-Hyun Yun),조수민(Su-Min Cho),함병주(Byung-Ju Ham) 대한사회정신의학회 2005 사회정신의학 Vol.10 No.1

연구 목적: 고등학생들의 학업 성취도와 성격-기질, 심리적 요인과의 상관관계를 알아보고 그들의 학업 성취도를 예견할 수 있는 요인을 탐색해보고자 하였다. 방 법: 인문계 고등학교 1학년에 재학 중인 205명의 남, 여학생을 대상으로 성격-기질과 심리적 요인들을 자기 보고형 설문지로 조사하 였고 이를 공통으로 실시한 모의고사 성적과 비교하여 고등학생의 성격-기질, 심리적 요인과 학업 성취도와의 관계를 조사하였다. 결 과: 1) 남학생의 경우 여학생보다 학업성취도(Grade-point averages, GPA) 점수가 유의하게 높았다. 2) 전체 학생을 대상으로 시행한 부분상관분석 결과 성격기질검사(Temperament and Character Inventory, TCI)의 기질 차원인 novelty seeking(NS)와 reward dependence(RD)가 GPA와 음의 상관관계를 나타내었고, persistence(P)는 GPA와 유의한 양의 상관관계를 나타내었다. 3) 남학생에서는 RD만이 GPA와 유의한 음의 상관관계를 나타내었다. 여학생의 경우 P와 State-Trait Anxiety Scale(STAIX)의 상태불안 점수가 GPA와 양의 상관관계를 나타내었다. 4) 회귀분석 결과, 남학생에서는 학업성취도를 가장 잘 설명해주는 변인은 RD(F=4.789, p<.05)로, 설명변량은 4.7%였으며, P(F=4.561, p<.05)가 추가됨으로써 설명변량은 8.6%로 증가되었다. 여학생의 경우 회귀분석상 상태불안 점수(F=5.610, p<.05) 만이 유의한 설명변인으로 분석되었다. 결 론: 인문계 고등학생의 GPA는 남학생에서 RD와 P가, 여학생에서 상태불안이 유의한 예언 변인이라는 것을 발견하였다. Objective:This study investigated the relationships between character-temperament and academic achievement in high school students. Method:The study subjects were 205 first-year students of a high school during one academic year(2004). Gradepoint averages(GPA) from the second semester of the first year of high school were obtained as a measure of academic achievement, and all participants completed the Temperament and Character Inventory, Beck depression Inventory, and State-Trait Anxiety Inventory. Results:In general, our results revealed novelty seeking and reward dependence(RD) were negatively correlated with GPA, and persistence(P) was positively correlated with GPA. If data were divided by gender, only RD was negatively correlated with GPA in boys, and P and state anxiety were positively correlated with GPA in girls. In stepwise regression anaysis, RD and P were significant predictive variables for GPA in boys, and trait anxiety was only predictive variable in girls. Conclusion:We were able to delineate relationships between character-temperament and academic achievement.

성인과 소아 아토피피부염 환자에서 가려움증의 임상적 특징

심우행 ( Woo Haing Shim ),송창현 ( Chang Hyun Song ),박현제 ( Hyun Je Park ),김훈수 ( Hoon Soo Kim ),진현우 ( Hyun Woo Chin ),김수한 ( Su Han Kim ),고현창 ( Hyun Chang Ko ),김문범 ( Moon Bum Kim ),김도원 ( Do Won Kim ),김병수 ( By 대한피부과학회 2011 대한피부과학회지 Vol.49 No.4

Background: Itch is an essential feature of atopic dermatitis (AD). Active AD cannot be diagnosed without a history of itch. There has been no previous study delineating the different characteristics of itch according to age. Objective: We wanted to characterize and compare the clinical patterns and the sensory and affective dimensions of itch in adult and childhood AD patients. Methods: A face-to-face structured questionnaire based on the McGill pain questionnaire was given to 90 patients with AD. The patients were classified into two groups: adult AD patients (≥15 years old) and childhood AD patients (<15 years old). Results: Eighty-eight percent of the total AD patients experienced itch everyday. This itch was mostly aggravated during the summer and at night in both groups of AD patients. The antecubital fossae, popliteal fossae and neck were the major sites of involvement with itch. More patients perceived the characteristics of itch as crawling, burning and tickling rather than stinging, stabbing and pinching. The majority of AD patients answered that sweat and hot climate aggravated the itch and medications and cold climate alleviated the itch. However, compared with the childhood patients, more adult patients experienced the aggravation of itch by exposure to dust, and the itch was alleviated by taking medications and by concentrating on work. A burning sensation with itch was more frequently reported by the adult patients when compared to that of the childhood patients. Conclusion: This study highlights the detailed description and characteristics of itch in adult and childhood AD patients. The proper management of itch according to age may be prerequisite to improve AD patients` quality of life. (Korean J Dermatol 2011;49(4):318∼327)

The Mildly Elevated Serum Bilirubin Level is Negatively Associated with the Incidence of End Stage Renal Disease in Patients with IgA Nephropathy

Chin, Ho Jun,Cho, Hyun Jin,Lee, Tae Woo,Na, Ki Young,Oh, Kook Hwan,Joo, Kwon Wook,Yoon, Hyung Jin,Kim, Yon-Su,Ahn, Curie,Han, Jin Suk,Kim, Suhnggwon,Jeon, En Sil,Jin, Dong Chan,Kim, Yong-Lim,Park, Sun The Korean Academy of Medical Sciences 2009 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.24 No.suppl

<P>Oxidative stress plays various roles in the development and progression of IgA nephropathy, while bilirubin is known as a potent antioxidant. We therefore hypothesized that serum bilirubin would be associated with renal prognosis in IgA nephropathy. The study subjects comprised 1,458 adult patients with primary IgA nephropathy in Korea. We grouped patients according to the following quartile levels of bilirubin: <0.4 mg/dL (Q1), 0.4-0.5 mg/dL (Q2), 0.6-0.7 mg/dL (Q3), and >0.8 mg/dL (Q4). The outcome data were obtained from the Korean Registry of end-stage renal disease (ESRD). Eighty patients (5.5%) contracted ESRD during a mean follow-up period of 44.9 months. The ESRD incidences were 10.7% in Q1, 8.2% in Q2, 2.8% in Q3, and 2.8% in Q4 (<I>p</I><0.001). The relative risk of ESRD compared to that in Q1 was 0.307 (95% confidence interval [CI], 0.126-0.751) in Q3 and 0.315 (95% CI, 0.130-0.765) in Q4. The differences of ESRD incidence were greater in subgroups of males and of patients aged 35 yr or more, with serum albumin 4.0 g/dL or more, with normotension, with eGFR 60 mL/min/1.73 m<SUP>2</SUP> or more, and with proteinuria less then 3+ by dipstick test. In conclusion, higher bilirubin level was negatively associated with ESRD incidence in IgA nephropathy.</P>

Germinal Virus Vector WDV(Wheat Dwarf Virus) : Mediated Multiple Insertions of a Maize Transposon, Ds(Dissociation), in Rice

Chin, Hang Gyeong,Park, Sung Han,Choe, Mi Sook,Park, Su Hyun,Oh, Byeong Keun,Lee, Gi Hwan,Choe, Hae Choon,Cho, Moo Je,Hong, Jong Chan,Han, Chang-Deok 한국식물학회 2000 Journal of Plant Biology Vol.43 No.1

Wheat dwarf virus (WDV) is a monocot-infecting geminivirus that replicates in infected tissue as double-stranded DMA. We evaluated whether the WDV vector system bearing Ds could be used as an effective insertional mutagen in rice. Molecular data showed that Ds was excised from WDV vectors once the WDV-carrying Ds (WDV::Ds) and the genomic Ac vector were co-introduced into rice calli. Mature T0 and T1 transgenic plants were analyzed for the distribution and inheritance of Ds inserts. Southern analysis indicated that the Ds elements excised from WDV vectors were stably inserted into genomes. The number of transposed Ds ranged from zero to three copies, among independent transformants. Meanwhile, untransposed Ds (WDV::Ds) were present in multiple-copies in genomes. Southern analysis of the selfed progeny of T0 plants demonstrated that most WDV::Ds were co-segregated among siblings. This indicated that these elements were integrated into the same single loci. However, a few Ds were found to segregate independently from the majority of Ds. In this report, we discuss the efficiency of WDV vectors in generating multicopy Ds in rice genomes.

Molecular Analysis of Rice Plants Harboring an Ac/Ds Transposable Element-Mediated Gene Trapping Systems

Chin, Hang-Gyeong,Choe, Mi-Sook,Lee, Sung-Ho,Park, Sung-Han,Koo, Ja-Choon,Kim, No-Youl,Park, Su-Hyun,Lee, Jeung Joo,Oh, Byeong-Geun,Yi, Gi-Hwan,Kim, Soon-Chul,Choi, Hae-Choon,Cho, Moo-Je,Han, Chang-de Gyeongsang National University Chinju, Korea 1998 Plant molecular biology and biotechnology research Vol.1998 No.

만성 신부전으로 발현된 Erdheim-Chester disease

황현석 ( Hyun Suk Hwang ),지병수 ( Byoung Su Ji ),이진국 ( Chin Kook Lee ),김진영 ( Jin Young Kim ),최범순 ( Bum Soon Choi ),양철우 ( Chul Woo Yang ),김용수 ( Yong Soo Kim ) 대한내과학회 2007 대한내과학회지 Vol.73 No.2

Erdheim-Chester disease is a rare non-langerhans cell histiocytic disorder of an unknown cause that may associate with multisystem, rapidly fatal, infiltrative disease. We report here on a case of Erdheim-Chester disease in a 45-year-old man who presented with chronic renal failure. The patient visited a local clinic due to renal failure that was accidentally diagnosed on a routine health examination. At that time, abdominal computed tomography revealed retroperitoneal fibrosis and bilateral hydronephrosis. However, he did not visit hospital for further evaluation. After 1 year, he visited our clinic due to exertional dyspnea that had developed several months previously. The chest and abdominal CT showed interstitial pneumonitis and pleural effusion and there was progressed hydronephrosis and perirenal fibrosis. He was diagnosed with Erdheim-Chester disease based on the characteristic findings of pleural biopsy (interstitial fibrosis, infiltration of macrophge, CD68-positive but S-100 negative). The patient was treated with insertion of a double J catheter and immunesuppression with prednisolone and azathioprine. Erdheim-Chester disease is a rare disease, but it should be considered in those patients displaying chronic renal failure with retroperitoneal fibrosis.(Korean J Med 73:216-222, 2007)