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In vitro fertilization using sex-sorted boar sperm mediated by magnetic nanoparticles
Hakjae Chung,Sunyoung Baek,Soojin Sa,Youngshin Kim,Joonki Hong,Eunseok Cho,Jihwan Lee,Seungmin Ha,Jungho Son,Seunghwan Lee,Inchul Choi,Kyungwoon Kim 충남대학교 농업과학연구소 2020 Korean Journal of Agricultural Science Vol.47 No.4
A deep learning-based approach for feeding behavior recognition of weanling pigs
( Minju Kim ),( Yohan Choi ),( Jeong-nam Lee ),( Soojin Sa ),( Hyun-chong Cho ) 한국축산학회 2021 한국축산학회지 Vol.63 No.6
Feeding is the most important behavior that represents the health and welfare of weanling pigs. The early detection of feed refusal is crucial for the control of disease in the initial stages and the detection of empty feeders for adding feed in a timely manner. This paper proposes a real-time technique for the detection and recognition of small pigs using a deep-leaning-based method. The proposed model focuses on detecting pigs on a feeder in a feeding position. Conventional methods detect pigs and then classify them into different behavior gestures. In contrast, in the proposed method, these two tasks are combined into a single process to detect only feeding behavior to increase the speed of detection. Considering the significant differences between pig behaviors at different sizes, adaptive adjustments are introduced into a you-only-look-once (YOLO) model, including an angle optimization strategy between the head and body for detecting a head in a feeder. According to experimental results, this method can detect the feeding behavior of pigs and screen non-feeding positions with 95.66%, 94.22%, and 96.56% average precision (AP) at an intersection over union (IoU) threshold of 0.5 for YOLOv3, YOLOv4, and an additional layer and with the proposed activation function, respectively. Drinking behavior was detected with 86.86%, 89.16%, and 86.41% AP at a 0.5 IoU threshold for YOLOv3, YOLOv4, and the proposed activation function, respectively. In terms of detection and classification, the results of our study demonstrate that the proposed method yields higher precision and recall compared to conventional methods.
Gu, Minsu,Ko, Seunghee,Yoo, Seungmin,Lee, Eunhee,Min, Sa Hoon,Park, Soojin,Kim, Byeong-Su Elsevier 2015 Journal of Power Sources Vol.300 No.-
<P><B>Abstract</B></P> <P>We present a fabrication of scalable coaxial core/shell silicon (Si)-graphene fiber prepared by dual-nozzle-induced wet-spinning assembly for high-performance Si anode. Over 50 wt% of Si nanoparticles mixed with graphene oxide suspension can be incorporated in the core with the outstanding dispersibility of unique silver-coated Si nanoparticles in aqueous media. The core fiber is further encapsulated by graphene shell which not only provides conducting pathways, but also alleviates severe volume expansion of Si core. This novel core/shell Si anode with double locked graphene architecture delivers more stable cycle performance and superior rate capability than anodes composed of simple mixture of Si-graphene composites.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Core/shell structured graphene fiber with silver-coated silicon was formed by wet-spinning. </LI> <LI> This study examined the battery performance depending on structural effect and different reduction conditions. </LI> <LI> We achieved a highly enhanced performance of capacity and excellent rate capabilities, comparing to simple mixture. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( Sun-Young Baek ),( Seung-Hoon Lee ),( Youngshin Kim ),( Joon-Ki Hong ),( Eunseok Cho ),( Seungmin Ha ),( Kyungwoon Kim ),( Soojin Sa ),( Hakjae Chung ) 한국축산학회 2021 한국축산학회지 Vol.63 No.5
Closely correlated expression patterns between ubiquitin specific peptidase 9X-linked (US-P9X) and adherens junction formation factor (Afadin) in mouse testis development suggests that Usp9x regulates the deubiquitination of Af-6 (also known as Afadin, AFDN), and subsequently, the cell adhesion dynamics during gametogenesis. However, this relationship has not yet been tested in other domestic animals. The study was examined the temporal and spatial expression patterns of porcine USP9X and AFDN from the pre-pubertal to adult stages using real time-PCR and immunohistochemistry. Furthermore, we detected the transcripts of USP9X and AFDN in the testis of 1-, 6- and 12-months old boar, respectively. USP9X and AFDN were found to have similar expressions patterns, with basal expression after 1 month followed by a significant up-regulation from 6 months (puberty) onwards. In addition, neither the AFDN or USP9X proteins were detected in spermatogenic cells but they were expressed in the leydig cells and sertoli cells. USP9X was detected around the basal lamina during pre-puberty, and predominantly expressed in the leydig cells at puberty. Finally, in adult testis, USP9X was increased at the sertoli cell-cell interface and the sertoli cell-spermatid interface. In summary, closely correlated expression patterns between USP9X and AFDN in boar testis supports the previous findings in mice. Furthermore, the junction connections between the sertoli cells may be regulated by the ubiquitination process mediated via USP9X.