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Baek, So-Hyeon,Kwon, In-Sook,Park, Tae-Il,Yun, Song-Joong,Kim, Jin-Key,Choi, Kyeong-Gu Korean Society for Biochemistry and Molecular Biol 2000 Journal of biochemistry and molecular biology Vol.33 No.5
Overwintering plants should survive the various biotic and abiotic stresses that occur during winter. Previous studies indicated that active oxygen species are involved in freezing, dehydration, anoxia and pathogen infections. As the importance of the events that occur in the intercellular compartment became apparent in disease resistance, we examined the nature of intercellular antioxidant enzymes in order to access their possible involvement in the winter hardiness of barley. The levels of intercellular peroxidase, catalase, and SOD activities on the unit protein basis were 394, 18, and 9% of those of cellular activities, respectively. Major intercellular peroxidase isoforms consisted of four neutrals and four basic forms; whereas major cellular isoforms were two basic forms. Out of the two major catalase isoforms a higher molecular weight form was predominantly abundant in both cellular and intercellular compartments. Among the five major cellular SOD isoforms, three were also present in the intercellular compartment. The presence of substantial amounts of intercellular antioxidant enzymes in overwintering barley leaves may suggest the involvement of these enzymes in the tolerance mechanism to the various stresses that occur during winter.
Development of salt-tolerant transgenic rice using OsCBF4 cDNA
So-Hyeon Baek,Eun-Mi Lee,Man-Kee Baek,Woo-Jae Kim,Jong-Ho Park,Ki-Yong Ha,Hyun-Soon Kim,Young-Chan Cho,Jeom-Ho Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
This study was conducted to isolate a salt tolerant gene and to develop salt tolerant rice for reclaimed-saline areas through genetic transformation. A rice c/DRE binding factor 4(OsCBF4) cDNA was isolated from rice using RT-PCR. The full-length cDNA of the CBF4 gene consists of 1,429 nucleotides and 274 amino acid residues. In order to develop salt tolerant rice, transgenic rice plants containing the OsCBF4 gene were obtained via Agrobacterium-mediated transformation. The stable incorporation of the OsCBF4 gene into rice genome was confirmed by PCR and Southern analysis. The stable expression of introduced gene was also validated by RT-PCR analysis in T2 plants. Biological assay of T3 progeny of the transgenic plants in Yoshida solution containing 120mM Nacl for 2weeks, confirmed that the OsCBF4 confers salt tolerance to transgenic rice plants. OsCBF4 transgene in the transgenic line CBF4-10 was markedly expressed up to over three-fold in the leaf by 120 mM NaCl treatment. Real-time PCR analysis revealed that the levels of the transgene expression were markedly increased under salt treatment. The transgenic line CBF4-10 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs
Yang Qin,So-Hyeon Baek,Soon-Jong Kweon,Taek-Ryoun Kwon,Myung-Ho Lim,Kong-Sik Shin,Hyun-Suk Cho,Hee-Jong Woo 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
A variety of genetically modified (GM) crops have been developed in Korea. In these crops, the resveratrol-enriched transgenic rice plant has moved ahead to generate the dossier for regulatory review process required for commercialization of GM crop. The resveratrol-enriched transgenic rice plant could be released to farmers for cultivation after national regulators have determined that it is safe for the environment and human health. Here we developed a PCR-based DNA marker based on flanking sequences of transgene for the discrimination of zygosity in resveratrol-enriched transgenic rice plant. This DNA marker will be useful for identifying of resveratrol-enriched transgenic rice plant, and can also be use to estimate transgene movement occurred by pollen transfer or seed distribution.
A new mid-late maturing rice variety「Subo」with a good grain quality and for direct seeding
Man-Kee Baek,Woon-Chul Shin,Jong-Cheol Ko,Bo-Kyeong Kim,Jeong-Kwon Nam,Ki-Yeong Kim,Mun-Sik Shin,Ki-Yong Ha,Hyeon-Su Park,Hyeon-Jung Kang,So-Hyun Baek,Young-Jun Mo,Jae-Kwon Ko,Woo-Jae Kim,Young-Bok Le 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
‘Subo’ is a new japonica rice variety developed by a cross breeding between HR21124-B-59 and HR19567-B-70-3 having a good Phenotype with a multi-resistance and high yield elite line. The new variety developed for direct seeding by the rice breeding team in Rice Breeding and Cultivation Research Division, Department of Rice and Winter Cereal Crop, NICS, RDA in 2012. The heading date of this variety is August 13 and earlier than that of check variety, Nampyeongbyeo, by two days. ‘Subo’ has 72cm of culm length and 104 spikelets per panicle in direct seeding cultivation. This variety showed resistance to bacterial leaf blight and rice stripe virus and have germinating ability in sumerged soil conditions. The milled rice of this variety exhibits translucent and very clear non-glutinous endosperm. ‘Subo’ has much better palatability of cooked rice than that of Nampyeongbyeo. The whole grain rate of milled rice are 86.9% and milled rice recovery of are 74.6%. The yield of ‘Subo’ in direct seeding cultivation is 5.55MT/ha in milled rice. ‘Subo’ could be adaptable to the southern part plain area of Pyeongtaek and southwestern in Korea.
Myoung Ryoul Park,So-Hyeon Baek,Benildo G. de los Reyes,Karl H. Hasenstein,Song Joong Yun 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Phosphorus (P) is an important structural component and plays critical roles in the process of energy transfer and signal transduction. Effect of low P on carbohydrate metabolism was investigated at the transcription level via transcriptome analysis using the rice 60K oligonucleotide DNA microarrays. Two-week-old rice seedlings were grown under a low (32 μM) or high (320 μM) P condition for two weeks and leaves from the seedlings were used for transcriptome analysis. Expression of genes involved in carbohydrate metabolic pathways (eg. glycolysis, sucrose degradation and starch synthesis and degradation) was most significantly affected under low P. Under low P, most genes involved in glycolysis were intensively down-regulated, genes of starch biosynthesis and degradation pathway were up- or down-regulated, and many genes involved in sucrose biosynthesis were intensively up-regulated. In leaves under low P, glucose and pyruvate levels decreased, but sucrose and starch levels increased. These results suggest that carbohydrate metabolism is adjusted primarily through comprehensive transcriptional modulation of genes involved in the carbohydrate metabolic super-pathway.
Soo-Kwon Park,So-Hyeon Baek,Dool-Yi Kim,Myoung-Ryoul Park,Na-Ra Lee,Kyoung Soon Shin,Su-Kyoung Jeon,Eun-Jae Kim,Sun-Lim Kim,Jung-Kyoung Moon 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Rice flour is used in many food products. However, dough made from rice lacks extensibility and elasticity, whereas that of wheat is suitable for many food products including breads. We have produced marker-free transgenic rice plants containing a wheat TaGlu-Ax1 gene encoding the HMG-GS from the Korean wheat cultivar ‘Jokyeong’ using the Agrobacteriummediated co-transformation method. The TaGlu-Bx7-own promoter was inserted into a binary vector for seed-specific expression of the TaGlu-Ax1 gene. Two expression cassettes comprised of separate DNA fragments containing only TaGlu-Ax1 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to the Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring TaGlu-Ax1 or HPTII was infected to rice calli at a 3:1 ratio of TaGlu-Ax1 and HPTII, respectively. Then, among 210 hygromycin-resistant T0 plants, we obtained 20 transgenic lines with both TaGlu-Ax1 and HPTII genes inserted into the rice genome. We reconfirmed integration of the TaGlu-Ax1 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the wheat TaGlu-Ax1 were stably expressed in the rice T1 seeds. Finally, the marker-free plants harboring only the TaGlu-Ax1 gene were successfully screened at the T1 generation.
A new mid-late maturing japonica rice variety Hyeonpum with a good grain quality and eating quality
Jeong-Ju Kim,Woon-Chul Shin,Mun-Sik Shin,Ki-Yeong Kim,Bo-Kyeong Kim,Jeong-Kwon Nam,Jong-Cheol Ko,Hyeon-Su Park,Ki-Yong Ha,Man-Kee Baek,So-Hyeon Baek,Young-Jun Mo,Woo-Jae Kim,Hyeon-Jung Kang,Jae-Kwon K 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
‘Hyeonpum’ is a new japonica rice variety developed by a cross breeding between Iksan469 having a good canopy architecture and Sindongjin and Musashino 7 having a good eating-quality with a view to develop a new variety having high quality of grain and palatability by the rice breeding team of Rice Breeding and Cultivation Research Division, Department of Rice and Winter Cereal Crop, NICS, RDA in 2012. The heading date of this variety is August 18 and later than that of check variety, Nampyeongbyeo, by four days. ‘Hyeonpum’ has 74cm of culm length and 101 spikelets per panicle. This variety showed resistance to bacterial leaf blight and rice stripe virus, but susceptible to leaf blast and planthoppers. The milled rice of this variety exhibits translucent and very clear non-glutinous endosperm. ‘Hyeonpum’ has much better palatability of cooked rice than that of Nampyeongbyeo. The whole grain rate of milled rice and milled rice recovery of ‘Hyeonpum’ are slightly higher than those of Nampyeongbyeo as 91.8% and 75.2% respectively. The yield of ‘Hyeonpum’ is 5.6 MT/ha in milled rice. ‘Hyeonpum’ could be adaptable to the southern part plain area of Pyeongtaek and southwestern costal areas in Korea.
백지현,박은영,정윤숙,홍재원,채윤태,진성준,최희경,신소연,한상훈,진범식,김창오,최준용,송영구,조남훈,김준명 대한감염학회 2009 감염과 화학요법 Vol.41 No.4
Coccidioidomycosis is a fungal infection that results from inhaling the airborne arthroconidia of the Coccidioides species. It is an endemic disease in the southwest part of North America and rarely diagnosed in Korea. As tourism to endemic areas and the number of immunocompromised patients have been increasing, the incidence of this infection has increased in non-endemic areas. Treatment is usually successful with antifungal agents; however, recurrence is common. It is difficult to decide when to discontinue the antifungal treatment especially in non-endemic areas where doctors are not familiar with the disease. We report a case of recurrent coccidioidomycosis manifesting as osteomyelitis after the treatment of the patient for disseminated coccidioidal infection. The complement fixation test was a useful tool for the assessment of patient response and to evaluate suspected recurrence.