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Effect of Portal Vein Chemotherapy on Liver Metastasis after Surgical Resection of Colorectal Cancer
Yu, Dong-Sheng,Li, Ying,Huang, Xin-En,Lu, Yan-Yan,Wu, Xue-Yan,Liu, Jin,Cao, Jie,Xu, Xia,Xiang, Jin,Wang, Guo-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9
Objective: To explore the effect of portal vein chemotherapy on liver metastasis after surgical resection of colorectal cancer. Methods: Patients fulfilling the eligibility criteria were assigned to receive either surgery plus 1-week continuous infusion of 5-FU (study group) or surgery alone (observational group). Patients in the study group received portal vein chemotherapy, whereby 5-FU (1000 mg/d) and heparin (5000 IU/d) infusion was initiated from the day of surgery and lasted for 7 consecutive days. Liver metastasis was monitored during five years follow-up postoperatively. Results: Sixty four patients were recruited and assigned to the study group (12 with colon and 20 with rectal cancer) or the control group (10 with colon and 22 with rectal cancer). Liver metastasis rate was 12.5% in study and 25.0% in observational group, the difference being significant (P<0.01). Conclusion: Portal vein chemotherapy could be an effective treatment in preventing liver metastasis after surgical resection of colorectal cancer.
Clozapine Induces Apoptosis in HIT-T15 Pancreatic β-Cell Lines
Jin, Sheng Yu,Chung, Joo-Ho,Guak, Cheol-Woo,Kim, Mi-Kyoung,Kim, Jong-Woo 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2
목 적 : Clozapine의 드문 부작용으로 췌장염이나 고혈당증 등이 보고되고 있지만 아직까지 정학한 기전을 밝혀지지 않고 있다. 본 연구는 이러한 부작용과 관련하여 clozapine이 췌장 조직에 직접적인 세포 독성을 일으킬 것이라는 가설 하에, clozapine이 HIT-T15 췌장 β-세포주에서 세포 사멸 과정인 apoptosis를 유도하는지를 알아보고자 하였다. 방 법 : 먼저 MTT assay로 HIT-T15 췌장 β-세포주에서 clozapine의 세포 독성을 알아보고 형태학적 변화를 관찰하였으며 이러한 세포 독성의 형태로 apoptosis가 일어나는지를 DAPI staining, TUNEL assay, caspase-3 assay 등의 방법을 사용하여 알아 보았다. 결 과 : Clozapine이 HIT-T15 췌장 β-세포주에서 농도와 시간 의존 양상으로 세포 독성을 일으키고, 형태학적 관찰, DAPI staining, TUNEL assay 결과에서 apoptosis의 특징적인 소견을 나타내었다. 그리고 clozapine 처치 시 caspase-3의 활성이 유의하게 증가되어, caspase-3에 의해 매개되어 apoptosis가 유도된다는 것을 알 수 있었다. 결 론 : 본 연구결과로 볼 때, HIT-T15 췌장 β-세포주에서 clozapine에 의해 유도되는 apoptosis가 clozapine에 의해 발생되는 췌장염이나 고혈당증 같은 부작용의 병태생리학적 기전에 관여할 가능성이 있을 것으로 생각한다.
Association of estrogen receptor 1 intron 1 C/T polymorphism in Korean vitiligo patients
Jin, Sheng-Yu,Park, Hyun-Ho,Li, Guang-Zhe,Lee, Hee Jae,Hong, Mee-Suk,Park, Hae-Jeong,Park, Hun-Kuk,Seo, Jung-Chul,Yim, Sung-Vin,Chung, Joo-Ho,Lee, Mu-Hyoung KYUNG HEE UNIVERSITY MEDICAL CENTER 2005 고황의학상 수상논문집 Vol.21-22 No.-
Background: Vitiligo is a common disease characterized by cutaneous white maculae due to loss of melanocytes. It is a polygenic disease, however, the exact pathogenesis of vitiligo is not yet known. The estrogen receptor (ESR) 1 gene was selected as a candidate gene because some researchers treated vitiligo successfully with the steroid-thyroid hormone mixture containing estrogen. Furthermore ESR was expressed in the melanocytes which have an important role in the pigmentation. The polymorphisms of ESR1 gene in vitiligo patients was not reported yet. Objective: To determine whether polymorphisms of ESR1 gene were associated with susceptibility to vitiligo patients in Korean population. Methods: We conducted case-control association study of vitiligo patients (120) and healthy controls (254). Genotypes of ESR1 gene (intron 1 C/T, exon 4 C/G, and exon 8 A/G) were determined by polymerase chain reaction followed by restriction enzyme digestion. Results: Intron 1 T/C allele frequency was significantly different between patients and controls (P=0.034). Intron 1 T/C genotype distribution (P=0.021) and allele frequency (P = 0.013) were different between female vitiligo patients and female controls. Intron 1 T/C allele frequency showed significantly difference between generalized type of vitiligo patients and controls (P=0.044). Genotype distributions and allele frequencies of exon 4 C/G and exon 8 A/G polymorphisms were not different between patients and controls. Conclusion: The present study suggests that ESR1 may be a possible risk factor for female or generalized type of vitiligo patients.
Association of estrogen receptor 1 intron 1 C/T polymorphism in Korean vitiligo patients
Jin, Sheng-Yu,Park, Hyun-Ho,Li, Guang-Zhe,Lee, Hee Jae,Hong, Mee-Suk,Park, Hae-Jeong,Park, Hun-Kuk,Seo, Jung-Chul,Yim, Sung-Vin,Chung, Joo-Ho,Lee, Mu-Hyoung KYUNG HEE UNIVERSITY MEDICAL CENTER 2005 고황의학상 수상논문집 Vol.Vol.21-22 No.-
Clozapine Induces Apoptosis in HIT-T15 Pancreatic β-Cell Lines
Sheng Yu Jin(김성우),Joo-Ho Chung(정주호),Cheol-Woo Guak(곽철우),Mi-Kyoung Kim(김미경),Jong-Woo Kim(김종우) 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2
목적 : Clozapine의 드문 부작용으로 췌장염이나 고혈당증 등이 보고되고 있지만 아직까지 정확한 기전은 밝혀지지 않고 있다. 본 연구는 이러한 부작용과 관련하여 clozapine이 췌장 조직에 직접적인 세포 독성을 일으킬 것이라는 가설 하에, clozapine이 HIT-T15 췌장 β-세포주에서 세포 사멸 과정인 apoptosis를 유도하는지를 알아보고자 하였다. 방법 : 먼저 MTT assay로 HIT-T15 췌장 β-세포주에서 clozapine의 세포 독성을 알아보고 형태학적 변화를 관찰하였으며 이러한 세포 독성의 형태로 apoptosis가 일어나는지를 DAPI staining TUNEL assay, caspase-3 assay 등의 방법을 사용하여 알아 보았다. 결과 : Clozapine이 HIT-T15 췌장 β-세포주에서 농도와 시간 의존 양상으로 세포 독성을 일으키고, 형태학적 관찰, DAPI staining, TUNEL assay 결과에서 apoptosis의 특징적인 소견을 나타내었다. 그리고 clozapine 처치 시 caspase-3의 활성이 유의하게 증가되어, caspase-3에 의해 매개되어 apoptosis가 유도된다는 것을 알 수 있었다. 결론 : 본 연구결과로 볼 때, HIT-T15 췌장 β-세포주에서 clozapine에 의해 유도되는 apoptosis가 clozapine 에 의해 발생되는 췌장염이나 고혈당증 같은 부작용의 병태생리학적 기전에 관여할 가능성이 있을 것으로 생각한다.
Zhang, Jin Yu,Dong, Huan Sheng,Oqani, Reza K,Lin, Tao,Kang, Jung Won,Jin, Dong Il BioScientifica 2014 Reproduction Vol.148 No.1
<P>Cell-to-cell contact mediated by cell adhesion is fundamental to the compaction process that ensures blastocyst quality during embryonic development. In this study, we first showed that Rho-associated coiled-coil protein kinases (ROCK1 and ROCK2) were expressed both in porcine oocytes and IVF preimplantation embryos, playing different roles in oocytes maturation and embryo development. The amount of mRNA encoding ROCK1 and the protein concentration clearly increased between the eight-cell and morula stages, but decreased significantly when blastocysts were formed. Conversely, ROCK2 was more abundant in the blastocyst compared with other embryonic stages. Moreover, immunostaining showed that ROCK1 protein distribution changed as the embryo progressed through cleavage and compaction to the morula stage. Initially, the protein was predominantly associated with the plasma membrane but later became cytoplasmic. By contrast, ROCK2 protein was localized in both the cytoplasm and the spindle rotation region during oocyte meiosis, but in the cytoplasm and nucleus as the embryo developed. In addition, ROCK2 was present in the trophectoderm cells of the blastocyst. Treatment with 15 μM Y27632, a specific inhibitor of ROCKs, completely blocked further development of early four-cell stage embryos. Moreover, we did not detect the expression of <I>ROCK1</I> but did detect <I>ROCK2</I> expression in blastocysts. Moreover, lysophosphatidic acid an activator of ROCKs significantly improved the rates of blastocyst formation. These data demonstrate that ROCKs are required for embryo development to the blastocyst stage. Together, our results indicate that ROCK1 and ROCK2 may exert different biological functions during the regulation of compaction and in ensuring development of porcine preimplantation embryos to the blastocyst stage.</P>
Yu-Qi He,Xin Wang,Lang Yang,Jian Zhang,Qian Kang,Shan Tang,Peng Jin,Jian-Qiu Sheng,Ai-Qin Li 대한소화기내시경학회 2015 Clinical Endoscopy Vol.48 No.5
Background/Aims: Early colorectal (CR) neoplasm can be cured by endoscopic submucosal dissection (ESD), but clinical experience and factors associated with complications from ESD for CR neoplasms in China have not been reported . Methods: Seventy-eight cases of early CR neoplasm treated with endoscopic resection performed between December 2012 and December 2013 at Beijing Military General Hospital were included. Factors associated with ESD complications and procedure times were evaluated. Results: The en bloc resection rate was 88.5% (69/78), tumor size was 32.1±10.7 mm, and procedure time was 71.8±49.5 minutes. The major complication was perforation, which occurred in 8.97% of the ESD procedures. Multivariate logistic regression analysis indicated that only tumor size (p=0.022) was associated with ESD perforation. Tumor size (p<0.001) and the non-lifting sign (p=0.017) were independent factors for procedure time, and procedure time (p=0.016) was a key factor for en bloc resection. After a median 10 months (range, 4 to 16) of follow-up, no patients had local recurrence. Conclusions: This study indicated that ESD is an applicable method for large early CR neoplasm in the colon and rectum. Tumor size and the non-lifting sign might be considerable factors for increased complication rate and procedural time of ESD.