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Liu Shaohua,Li Junhao,Li Na,Zhou Peng,Li Liulin 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.6
Calcium-dependent protein kinases (CDPKs) play signifi cant roles in response to environmental stresses in plants. However, the CDPK gene family in pear ( Pyrus bretschneideri Rehd) has not been thoroughly explored. In this study, we performed a comprehensive analysis of CDPK family genes in pear, and 30 CDPK genes were identifi ed, unevenly distributed across 13 chromosomes, and predicted to be mainly localized at the plasma membrane. Based on phylogenetic analysis and struc- tural features, the PbCDPKs were classifi ed into four distinct subfamilies. In addition, many putative cis-acting elements responsive to hormones and environmental stresses were identifi ed in the promoter regions. According to RNA-seq data, the majority of PbCDPK genes showed diff erential expression in diff erent pear tissues. After inoculation of plants with the fungus Venturia nashicola , four PbCDPK genes were diff erentially expressed in resistant and susceptible pear cultivars, suggesting that they might play an important role in resistance to the pear scab pathogen. These candidate genes will be subjected to disease resistance and functional analysis in future work. Our results thus provide a genome-wide characterization of CDPK family genes in pear and lay the foundation for further functional analysis.
Biodiesel production from the esterification of fatty acid over organophosphonic acid
Wei Liu,Xiguang Liu,Shaohua Zhang,Rongjun Qu 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1
Biodiesel production from the esterification of fatty acid (stearic acid) with ethanol catalyzed by PA/NaY(PA = organophosphonic acid, NaY = NaY molecular sieve) was investigated, and the effects of PA loading,catalyst amount, molar ratio of ethanol to acid, reaction time and temperature on the esterificationreaction were examined. The optimumvalues for maximum esterification percentage can be obtained byusing a Box–Behnken center-united design with a minimum of experimental work. The pseudohomogeneous(PH) model has been used to simulate the experimental data, and the calculated values ofArrhenius coefficient and activation energy are 6.394 103 and 70.51 kJ/mol, respectively.
Purification and Characterization of an Antioxidant Protein from Fertilized Eggs
Shaohua Yang,Zhengjiang Tang,ShanShan Tang,Tingfang Zhang,Fei Tang,Yu Wu,Ying Wang,Lulu Wang,Guoqing Liu 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.6
Free radicals may attack cells or tissue, leading to chronic diseases, and antioxidant consumption is potentially useful for removing free radicals. Egg proteins may be used as potential sources of antioxidant considering their ability of scavenging free radicals to apply for food or cosmetics industry. In this study, we obtained a natural antioxidant protein from fertilized eggs, which was a dietary supplement in some Asian countries. Meanwhile, antioxidant activities of these proteins were evaluated using different oxidation systems. With increasing incubation time, the antioxidant activity of these proteins increased during 15 d of incubation. The samples on day 15 were performed for isolation of antioxidant protein. The protein, named P4-1 (MW, 45 kDa), was isolated and purified by consecutive chromatographic methods. P4-1 contained 17 amino acids, which was determined by liquid chromatography-mass spectrometry and Amino Acid Analyzer. Moreover, the amino acid sequence was highly similar to that of ovalbumin. Differential scanning calorimetry showed that the denaturation temperature of P4-1 was 57.16ºC. Furthermore, P4-1 suggested high oxygen radical-absorbance activity in ·OH assays, and its antioxidant activity was stable at 30-50ºC in acidic and neutral pH. Thus, these results revealed that P4-1 may be a potential resource as a natural antioxidant.
Purification and Identification of a Natural Antioxidant Protein from Fertilized Eggs
Shaohua Yang,Lulu Wang,Ying Wang,Xiaoqian Ou,Zhaoyuan Shi,Chongchong Lu,Wei Wang,Guoqing Liu 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.5
Fertilized hen eggs are rich in a variety of bioactive ingredients. In this study, we aimed to obtain an antioxidant protein from fertilized eggs and the radical scavenging abilities on 1, 1- diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (•OH), superoxide anion (O2-•) were used to evaluate the antioxidant activity of the purified protein. During 20 d of incubation, the radical scavenging ability of protein extracted from fertilized eggs exhibited significantly differences and the protein on day 16 showed higher antioxidant capacity. Based on this, the antioxidant protein of the samples on day 16 were isolated for the follow-up study. With a molecular weight 43.22 kDa, the antioxidant protein was purified by Diethylaminoethyl cellulose -52 (DEAE-52) column and Sephadex G-100. The LC-MS analysis showed that the purified protein molecular weight was 43.22 kDa, named D2-S. The sequence of amino acids was highly similar to ovalbumin and the coverage reached to 84%. The purified protein showed a radical scavenging rate of 52.34±3.27% on DPPH and 63.49±0.25% on •OH, respectively. Furthermore, the C-terminal amino acid sequence was NAVLFFGRCVSP, which was consistent with the sequence of ovabumin. These results here indicated that purified protein may be a potential resource as a natural antioxidant.
Peripheral Insulin Doesn't Alter Appetite of Broiler Chicks
Liu, Lei,Xu, Shaohua,Wang, Xiaojuan,Jiao, Hongchao,Lin, Hai Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.9
An experiment was conducted to investigate the effect of peripheral insulin treatment on appetite in chicks. Six-d-age chicks with ad libitum feeding or fasting for 3 h before injection received a subcutaneous injection of 0, 1, 3, 5, 10, or 20 IU of insulin or vehicle (saline). The results showed peripheral insulin treatment (1 to 20 IU) did not alter significantly the feed intake in chicks under either ad libitum feeding or fasting conditions within 4 h (p>0.05). Compared with the control, plasma glucose concentration was significantly decreased after insulin treatment of 3, 5, 10, and 20 IU for 4 h in chicks with ad libitum feeding (p<0.05). In fasted chicks, 10 and 20 IU insulin treatments significantly decreased the plasma glucose level for 4 h (p<0.05). Peripheral insulin treatment of 10 IU for 2 or 4 h did not significantly affect the hypothalamic genes expression of neuropeptide Y, proopiomelanocortin, corticotropin-releasing factor and insulin receptors (p>0.05). All results suggest peripheral administration of insulin has no effect on appetite in chicks.
Shaohua Chen,Xuan Liu,Shengyang Dai,Wenfeng Xiang,Haizhong Guo,Junjian Li 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.76 No.2
CuxO (Cupric Oxide) nanotubes (NTs) were synthesized by using thermal oxidation of Cu nanowires (NWs) for 2 and 4 hours, respectively, and the effect of the components on their photoelectrical properties due to the infrared (IR) light with a wavelength of 1064 nm were investigated. In the 2-h CuxO NTs, the main component was Cu2O; however, the 4-h CuxO NTs were composed of a mixture of CuO and Cu2O. The photoelectric properties showed that the voltage difference and the sensitivity of 4-h CuxO NTs were more than 3 times those of the 2-h CuxO NTs, and its linear range was only about one-third that of the 2-h CuxO NTs. The above results were caused by the 4-h CuxO NTs containing a higher content of CuO, which had a suitable bandgap of 1.2 eV for light with a wavelength of 1064 nm. In addition, the response and the recovery times for the 4-h NTs were on the same order as those of the 2-h NTs. The conclusions from this article indicated that 4-h and 2-h NTs were very promising for the design of IR photodetector device with higher sensitivity and a larger detection range respectively.
Hiu Liu,Nana Xin,Qingxi Cao,Long Sha,Dezhi Sun,Shaohua Wu 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.4
The Computational fluid dynamics (CFD) code PHOENICS is applied to simulate and evaluate the combustion process within the furnace of a 1,000MW dual circle tangential firing single furnace lignite-fired ultra supercritical (USC) boiler. The dependence on overfire air (OFA) positioning on the combustion process is studied. The results show that the highest temperature appears on the upside of the burner zone close to the front wall, and the high temperature zone rises with elevated OFA positions. However, the temperature field distributions are similar despite differing OFA positions. The char content near the rear wall is higher than that near the front wall, and below the furnace arch, coal particles concentrate towards the front wall. Also with elevated OFA positions, nitrogen oxide (NOx) concentrations at the outlet fall, but char content increases. In regard to NOx emission and char burnout, the suggested optimal distance from the OFA center to the center of the uppermost primary air nozzle should be 6 meters.