In Vitro Antifungal Activity of (1)-N-2- Methoxybenzyl-1,10-phenanthrolinium Bromide against Candida albicans and Its Effects on Membrane Integrity

( Setiawati Setiawati ),( Titik Nuryastuti ),( Ngatidjan Ngatidjan ),( Mustofa Mustofa ),( Jumina Jumina ),( Dhina Fitriastuti ) 한국균학회 2017 Mycobiology Vol.45 No.1

Metal-based drugs, such as 1,10-phenanthroline, have demonstrated anticancer, antifungal and antiplasmodium activities. One of the 1,10-phenanthroline derivatives compounds (1)-N-2-methoxybenzyl-1,10-phenanthrolinium bromide (FEN), which has been demonstrated an inhibitory effect on the growth of Candida spp. This study aimed to explore the in vitro antifungal activity of FEN and its effect on the membrane integrity of Candida albicans. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of FEN against planktonic C. albicans cells were determined using the broth microdilution method according to the Clinical and Laboratory Standards Institute guidelines. Cell membrane integrity was determined with the propidium iodide assay using a flow cytometer and were visualized using scanning electron microscopy (SEM). Planktonic cells growth of C. albicans were inhibited by FEN, with an MIC of 0.39-1.56 μg/mL and a MFC that ranged from 3.125 to 100 μg/mL. When C. albicans was exposed to FEN, the uptake of propidium iodide was increased, which indicated that membrane disruption is the probable mode of action of this compound. There was cells surface changes of C. albicans when observed under SEM.

Model of Water, Energy and Waste Management for Development of Eco-Innovation Park ; A Case Study of Center for Research of Science and Technology "PUSPIPTEK," South Tangerang City, Indonesia

Setiawati, Sri,Alikodra, Hadi,Pramudya, Bambang,Dharmawan, Arya Hadi World Technopolis Association 2014 World Technopolis Review Vol.3 No.2

Center for Research of Science and Technology ("PUSPIPTEK") has 460 hectares land area, still maintained as a green area with more than 30% green space. There are 47 centers for research and testing technology, technology-based industries, and as well as public supporting facilities in PUSPIPTEK area. Based on the concepts developed to make this area as an ecological region, PUSPIPTEK can be seen as a model of eco-innovation. The purpose of this research is to develop a model of water, energy and waste management with eco-innovation concept. As a new approach in addressing environmental degradation and maintaining the sustainability of ecosystem, studies related to eco-innovation policy that combines the management of water, energy and waste in the region has not been done. In order to achieve the objectives of the research, a series of techniques for collecting data on PUSPIPTEK existing conditions will be carried out, which includes utilities data (water, electricity, sewage) and master plan of this area. The savings over the implementation of the concept of eco-innovation in water, energy, and waste management were calculated and analyzed using quatitative methods. The amount of cost savings and feasibility were then calculated. Eco innovation in water management among other innovations include the provision of alternative sources of water, overflow of rain water and water environments utilization, and use of gravity to replace the pumping function. Eco-innovation in energy management innovations include the use of LED and solar cell for air conditioning. Eco-innovation in waste management includes methods of composting for organic waste management. The research results: (1) The savings that can be achieved with the implementation of eco innovation in the water management is Rp. 3,032,640 daily, or Rp.1,106,913,600 annually; (2) The savings derived from the implementation of eco innovation through replacement of central AC to AC LiBr Solar Powered will be saved Rp.1,933,992,990 annually and the use of LED lights in the Public street lighting PUSPIPTEK saved Rp.163,454,433 annually; (3) Application of eco innovation in waste management will be able to raise awareness of the environment by sorting organic, inorganic and plastic waste. Composting and plastic waste obtained from the sale revenue of Rp. 44,016,000 per year; (4) Overall, implementation of the eco-innovation system in PUSPIPTEK area can saves Rp. 3,248,377,023 per year, compared to the existing system; and (5)The savings are obtained with implementation of eco-innovation is considered as income. Analysis of the feasibility of the implementation of eco-innovation in water, energy, and waste management in PUSPIPTEK give NPV at a 15% discount factor in Rp. 3,895,228,761; 23.20% of IRR and 4.48 years of PBP. Thus the model of eco-innovation in the area PUSPIPTEK is feasible to implement.

Future Research Directions in the Design of Versatile Extracellular Matrix in Tissue Engineering

Setiawati, Agustina,Nguyen, Huong Thanh,Jung, Yeongheon,Shin, Kwanwoo Korean Continence Society 2018 International Neurourology Journal Vol.22 No.S2

<P>Native and artificial extracellular matrices (ECMs) have been widely applied in biomedical fields as one of the most effective components in tissue regeneration. In particular, ECM-based drugs are expected to be applied to treat diseases in organs relevant to urology, because tissue regeneration is particularly important for preventing the recurrence of these diseases. Native ECMs provide a complex <I>in vivo</I> architecture and native physical and mechanical properties that support high biocompatibility. However, the applications of native ECMs are limited due to their tissue-specificity and chemical complexity. Artificial ECMs have been fabricated in an attempt to create a broadly applicable scaffold by using controllable components and a uniform formulation. On the other hands, artificial ECMs fail to mimic the properties of a native ECM; consequently, their applications in tissues are also limited. For that reason, the design of a versatile, hybrid ECM that can be universally applied to various tissues is an emerging area of interest in the biomedical field.</P>

Celecoxib, a COX-2 Selective Inhibitor, Induces Cell Cycle Arrest at the G2/M Phase in HeLa Cervical Cancer Cells

Setiawati, Agustina Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Celecoxib, a selective inhibitor of COX-2, showed cytotoxic effects in many cancer cell lines including cervical cancer cells. This study investigated the effect of celecoxib on cell cycle arrest in HeLa cervical cancer cells through p53 expression. In vitro anticancer activity was determined with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method. A double staining method was applied to investigate the mechanism of cell death, cell cycling was analyzed by flow cytometryand immunocytochemistry was employed to stain p53 expression in cells. Celecoxib showed strong cytotoxic effects and induced apoptosis with an $IC_{50}$ value of $40{\mu}M$. It induced cell cycle arrest at G2/M phase by increasing level of p53 expression on HeLa cells.

Model of Water, Energy and Waste Management for Development of Eco-Innovation Park ; A Case Study of Center for Research of Science and Technology “PUSPIPTEK,” South Tangerang City, Indonesia

Sri Setiawati,Hadi Alikodra,Bambang Pramudya,Arya Hadi Dharmawan 세계과학도시연합 2014 World Technopolis Review Vol.3 No.2

Center for Research of Science and Technology (“PUSPIPTEK”) has 460 hectares land area, still maintained as a green area with more than 30% green space. There are 47 centers for research and testing technology, technology-based industries, and as well as public supporting facilities in PUSPIPTEK area. Based on the concepts developed to make this area as an ecological region, PUSPIPTEK can be seen as a model of eco-innovation. The purpose of this research is to develop a model of water, energy and waste management with eco-innovation concept. As a new approach in addressing environmental degradation and maintaining the sustainability of ecosystem, studies related to eco-innovation policy that combines the management of water, energy and waste in the region has not been done. In order to achieve the objectives of the research, a series of techniques for collecting data on PUSPIPTEK existing conditions will be carried out, which includes utilities data (water, electricity, sewage) and master plan of this area. The savings over the implementation of the concept of eco-innovation in water, energy, and waste management were calculated and analyzed using quatitative methods. The amount of cost savings and feasibility were then calculated. Eco innovation in water management among other innovations include the provision of alternative sources of water, overflow of rain water and water environments utilization, and use of gravity to replace the pumping function. Eco-innovation in energy management innovations include the use of LED and solar cell for air conditioning. Eco-innovation in waste management includes methods of composting for organic waste management. The research results: (1) The savings that can be achieved with the implementation of eco innovation in the water management is Rp. 3,032,640 daily, or Rp.1,106,913,600 annually; (2) The savings derived from the implementation of eco innovation through replacement of central AC to AC LiBr Solar Powered will be saved Rp.1,933,992,990 annually and the use of LED lights in the Public street lighting PUSPIPTEK saved Rp.163,454,433 annually; (3) Application of eco innovation in waste management will be able to raise awareness of the environment by sorting organic, inorganic and plastic waste. Composting and plastic waste obtained from the sale revenue of Rp. 44,016,000 per year; (4) Overall, implementation of the eco-innovation system in PUSPIPTEK area can saves Rp. 3,248,377,023 per year, compared to the existing system; and (5)The savings are obtained with implementation of eco-innovation is considered as income. Analysis of the feasibility of the implementation of eco-innovation in water, energy, and waste management in PUSPIPTEK give NPV at a 15% discount factor in Rp. 3,895,228,761; 23.20% of IRR and 4.48 years of PBP. Thus the model of eco-innovation in the area PUSPIPTEK is feasible to implement.

Analysing the Influence of Technology on the Business Performance of Rattan Processing SME's in South Kalimantan

Iwan Inrawan Wiratmadja,Rajesri Govindaraju,Evy Setiawati 대한산업공학회 2011 Industrial Engineeering & Management Systems Vol.10 No.2

Technology is one of the major competitive advantages for small and medium enterprises (SME), especially ones operating in the manufacturing sector. Increasing technological capabilities is the basis for SME"s business performance improvement. The main problem of SME"s is a limitation in the areas of production facilities, technology, and human resources. Some of these constraints cause a decline in business performance and competitiveness of SME"s. In this case analysis of technology components has to be carried out to determine the effect of technology on SME"s business performance improvement. This study aims to measure the components of technology and to analyze the influence of each technology component on business performance of rattan processing SME"s in South Kalimantan. The assessment is carried out on the technoware, humanware, inforware, and orgaware components using the technometric method (UNESCAP). Business performance is measured through a combination of financial and non-financial aspects deducted from financial and marketing figures. Analysis of the influence of technology components on business performance of SME"s is done using the Structural Equation Model (SEM) with Partial Least Square software (PLS). Data is collected through interviews and questionnaires from 21 rattan processing SME"s in South Kalimantan that produce rattan furnitures. The results show that the value of the contribution of technology (TCC) to the performance of rattan processing SME"s in South Kalimantan is still quite low. Analysis of the results shows a direct influence of technoware and humanware on business performance, while orgaware influences business performance indirectly through humanware.

Analysing the Influence of Technology on the Business Performance of Rattan Processing SME's in South Kalimantan

Wiratmadja, Iwan Inrawan,Govindaraju, Rajesri,Setiawati, Evy Korean Institute of Industrial Engineers 2011 Industrial Engineeering & Management Systems Vol.10 No.2

Technology is one of the major competitive advantages for small and medium enterprises (SME), especially ones operating in the manufacturing sector. Increasing technological capabilities is the basis for SME's business performance improvement. The main problem of SME's is a limitation in the areas of production facilities, technology, and human resources. Some of these constraints cause a decline in business performance and competitiveness of SME's. In this case analysis of technology components has to be carried out to determine the effect of technology on SME's business performance improvement. This study aims to measure the components of technology and to analyze the influence of each technology component on business performance of rattan processing SME's in South Kalimantan. The assessment is carried out on the technoware, humanware, inforware, and orgaware components using the technometric method (UNESCAP). Business performance is measured through a combination of financial and non-financial aspects deducted from financial and marketing figures. Analysis of the influence of technology components on business performance of SME's is done using the Structural Equation Model (SEM) with Partial Least Square software (PLS). Data is collected through interviews and questionnaires from 21 rattan processing SME's in South Kalimantan that produce rattan furnitures. The results show that the value of the contribution of technology (TCC) to the performance of rattan processing SME's in South Kalimantan is still quite low. Analysis of the results shows a direct influence of technoware and humanware on business performance, while orgaware influences business performance indirectly through humanware.

Performance Analysis of Various Advanced Oxidation Processes on COD Removal from Raw Petroleum Refinery Effluent

Sri Martini,Sharmeen Afroze,Mira Setiawati 대한환경공학회 2021 대한환경공학회지 Vol.43 No.7

Objectives : This comparative study investigated various methods of advanced oxidation processes (AOPs) that were separately conducted for treating raw petroleum refinery effluent regarding chemical oxygen demand (COD) removal. Methods : Fenton, photo-Fenton, TiO₂, ZnO, TiO₂/Ultra violet (UV), and ZnO/UV were performed individually for measuring the effect of light irradiation, treatment time, pH, catalysts dosage, and light source on the profile of COD values. Results and Discussion : The experimental data of this work showed that the dependency on the light exposure in heterogeneous photo-catalytic reaction using TiO₂ and ZnO is higher than that of homogeneous photo-Fenton technique. The optimum operating conditions in heterogeneous system occurred at 100 min of oxidation time, pH5, and catalyst dosage 1 g/L that resulted in 21.8, 20.68, 60.9, and 55.17% of COD removal for TiO₂, ZnO, TiO₂/UV, and ZnO/UV, respectively. In contrast, both Fenton and photo-Fenton experienced their highest performance at pH 4 by obtaining 44.2 and 59.77% of COD removal, respectively. Eventually, kinetic study indicated that COD degradation can be well expressed by second-order pattern that reached higher correlation coefficient values by 0.999 and 0.998 for TiO₂/UV and TiO₂, respectively. Conclusions : Overall, it could be assumed that AOPs are reliable techniques to purify raw and complex raw industrial effluents.

Why and how do we keep editing local medical journals in an era of information overload?

Viera Wardhani,Sam Mathew,Jeong-Wook Seo,Komang G. Wiryawan,Vivi Setiawaty,Burmaajav Badrakh 한국과학학술지편집인협의회 2018 Science Editing Vol.5 No.2

In Silico Analysis of Potential Antidiabetic Phytochemicals from Matricaria chamomilla L. against PTP1B and Aldose Reductase for Type 2 Diabetes Mellitus and its Complications

Arisvia Sukma Hariftyani,Lady Aqnes Kurniawati,Siti Khaerunnisa,Anna Surgean Veterini,Yuani Setiawati,Rizki Awaluddin 한국생약학회 2021 Natural Product Sciences Vol.27 No.2

Type 2 diabetes mellitus (T2DM) and its complications are important noncommunicable diseases with high mortality rates. Protein tyrosine phosphatase 1B (PTP1B) and aldose reductase inhibitors are recently approached and advanced for T2DM and its complications therapy. Matricaria chamomilla L. is acknowledged as a worldwide medicinal herb that has many beneficial health effects as well as antidiabetic effects. Our research was designed to determine the most potential antidiabetic phytochemicals from M. chamomilla employing in silico study. 142 phytochemicals were obtained from the databases. The first screening employed iGEMdock and Swiss ADME, involving 93 phytochemicals. Finally, 30 best phytochemicals were docked. Molecular docking and visualization analysis were performed using Avogadro, AutoDock 4.2., and Biovia Discovery Studio 2016. Molecular docking results demonstrate that ligand-protein interaction's binding affinities were -5.16 to -7.54 kcal/mol and -5.30 to -12.10 kcal/mol for PTP1B and aldose reductase protein targets respectively. In silico results demonstrate that M. chamomilla has potential antidiabetic phytochemical compounds for T2DM and its complications. We recommended anthecotulide, quercetin, chlorogenic acid, luteolin, and catechin as antidiabetic agents due to their binding affinities against both PTP1B and aldose reductase protein. Those phytochemicals' significant efficacy and potential as antidiabetic must be investigated in further advanced research.