Correlation Between Knee Muscle Strength and Maximal Cycling Speed Measured Using 3D Depth Camera in Virtual Reality Environment

Ye Jin Kim(Ye Jin Kim),Hye-seon Jeon(Hye-seon Jeon),Joo-hee Park(Joo-hee Park),Gyeong-Ah Moon(Gyeong-Ah Moon),Yixin Wang(Yixin Wang) 한국전문물리치료학회 2022 한국전문물리치료학회지 Vol.29 No.4

Background: Virtual reality (VR) programs based on motion capture camera are the most convenient and cost-effective approaches for remote rehabilitation. Assessment of physical function is critical for providing optimal VR rehabilitation training; however, direct muscle strength measurement using camera-based kinematic data is impracticable. Therefore, it is necessary to develop a method to indirectly estimate the muscle strength of users from the value obtained using a motion capture camera. Objects: The purpose of this study was to determine whether the pedaling speed converted using the VR engine from the captured foot position data in the VR environment can be used as an indirect way to evaluate knee muscle strength, and to investigate the validity and reliability of a camera-based VR program. Methods: Thirty healthy adults were included in this study. Each subject performed a 15-second maximum pedaling test in the VR and built-in speedometer modes. In the VR speedometer mode, a motion capture camera was used to detect the position of the ankle joints and automatically calculate the pedaling speed. An isokinetic dynamometer was used to assess the isometric and isokinetic peak torques of knee flexion and extension. Results: The pedaling speeds in VR and built-in speedometer modes revealed a significantly high positive correlation (r = 0.922). In addition, the intra-rater reliability of the pedaling speed in the VR speedometer mode was good (ICC [intraclass correlation coefficient] = 0.685). The results of the Pearson correlation analysis revealed a significant moderate positive correlation between the pedaling speed of the VR speedometer and the peak torque of knee isokinetic flexion (r = 0.639) and extension (r = 0.598). Conclusion: This study suggests the potential benefits of measuring the maximum pedaling speed using 3D depth camera in a VR environment as an indirect assessment of muscle strength. However, technological improvements must be followed to obtain more accurate estimation of muscle strength from the VR cycling test.

Genome-wide identification of chemosensitive single nucleotide polymorphism markers in gastric cancer.

Ha, Ye Jin,Yoon, Sang Nam,Jeon, Yeo Jin,Cho, Dong Hyung,Roh, Seon Ae,Kim, Byung Sik,Kim, Hee Jin,Kim, Seon Young,Kim, Yong Sung,Kim, Jin Cheon Potamitis Press 2011 Anticancer research Vol.31 No.12

<P>A chemosensitive single nucleotide polymorphism (SNP) discovery schema is presented that utilizes (i) genome-wide SNP screening, with a human SNP array and an in vitro chemosensitivity assay, in 93 patients with gastric cancer (GC), and (ii) biological utility assessment using cell viability assays of transfected GC cells. Cytotoxicity analysis showed that most of the MKN1 and SNU638 clones transfected with the G allele of Deoxyribonuclease II beta (DNASE2B) rs3738573 were more sensitive to docetaxel than those with the C allele (p0.001-0.029) and most of the AGS and SNU638 clones transfected with the T allele of 5-hydroxytryptamine receptor IE (HTRIE) rs3828741 were more sensitive to paclitaxel than those with the C allele (p0.001-0.019). Our findings show that the two novel markers, DNASE2B rs3738573 and HTR1E rs3828741, have potential for improving the prediction of chemosensitivity of GC patients.</P>

Genomic and transcriptomic analysis of Korean colorectal cancer patients

Jeon Sol A,Ha Ye Jin,Kim Jong-Hwan,Kim Jeong-Hwan,Kim Seon-Kyu,Kim Yong Sung,Kim Seon-Young,Kim Jin Cheon 한국유전학회 2022 Genes & Genomics Vol.44 No.8

Background: Colorectal cancer (CRC) is the third most common type of diagnosed cancer in the world and has the second-highest mortality rate. Meanwhile, South Korea has the second-highest incidence rate for CRC in the world. Objective: To assess the possible influence of ethnicity on the molecular profile of colorectal cancer, we compared genomic and transcriptomic features of South Korean CRCs with European CRCs. Methods: We assembled a genomic and transcriptomic dataset of South Korean CRC patients (KOCRC; n = 126) from previous studies and European cases (EUCRC; n = 245) selected from The Cancer Genome Atlas (TCGA). Then, we compared the two datasets in terms of clinical data, driver genes, mutational signature, gene sets, consensus molecular subtype, and fusion genes. Results: These two cohorts showed similar profiles in driver mutations but differences in the mutation frequencies of some driver genes (including APC, TP53, PABPC1, FAT4, MUC7, HSPG2, GNAS, DENND5B, and BRAF). Analysis of hallmark pathways using genomic data sets revealed further differences between these populations in the WNT, TP53, and NOTCH signaling pathways. In consensus molecular subtype (CMS) analyses of the study cases, no BRAF mutations were found in the CMS1 subtype of KOCRC, which contrasts with previous findings. Fusion gene analysis identified oncogenic fusion of PTPRK-RSPO3 in a subset of KOCRC patients without APC mutations. Conclusions: This study presents insights into the genomic landscape of KOCRCs and reveals some similarities and differences with EUCRCs at the molecular level.

Clinical assessment and identification of immuno-oncology markers concerning the 19-gene based risk classifier in stage IV colorectal cancer

Lee, Jong Lyul,Roh, Seon Ae,Kim, Chan Wook,Kwon, Yi Hong,Ha, Ye Jin,Kim, Seon-Kyu,Kim, Seon-Young,Cho, Dong-Hyung,Kim, Yong Sung,Kim, Jin Cheon Baishideng Publishing Group Inc 2019 WORLD JOURNAL OF GASTROENTEROLOGY Vol.25 No.11

<P><B>BACKGROUND</B></P><P>Genomic profiling of tumors has contributed to the understanding of colorectal cancer (CRC), facilitating diagnosis, prognosis and selection of treatments, including targeted regimens. A report suggested that a 19-gene-based risk classifier (TCA19) was a prognostic tool for patients with stage III CRC. The survival outcomes in patients with stage IV CRC are still poor and appropriate selection of targeted therapies and immunotherapies is challenging.</P><P><B>AIM</B></P><P>To assess clinical implication of TCA19 in patients with stage IV CRC, and to identify TCA19 with involvement in immune-oncology.</P><P><B>METHODS</B></P><P>A retrospective review of the medical records of 60 patients with stage IV CRC was conducted, assessing clinicopathological variables and progression-free survival (PFS). TCA19 gene expression was determined by quantitative polymerase chain reaction (qPCR) in matched normal and tumor tissues taken from the study cohort. Expression of potential immune-oncology regulatory proteins and targets was examined by immunohistochemistry (IHC), western blot, immunofluorescence staining in tissues from a validation cohort of 10 patients, and in CRC cell lines co-cultured with monocyte <I>in vitro</I>.</P><P><B>RESULTS</B></P><P>In the patients with TCA19 score higher than the median, the PFS rates of eight patients who received the targeted regimens were significantly higher than the PFS rates of four patients who received 5-fluorouracil-based regimen (<I>P</I> = 0.041). In multivariate analysis, expression of signaling lymphocytic activation molecule family, member 7 (SLAMF7) and triggering receptor expressed on myeloid cells 1 (TREM1) was associated with PFS in the 60-patient cohort. After checking another 10 validate set, the expression of the IHC, the level of real-time qPCR, and the level of western blot were lower for SLAMF7 and higher for TREM7 in primary and metastatic tumors than in normal tissues. In CRC cells expressing SLAMF7 that were co-cultured with a monocytic cell line, levels of CD 68 and CD 73 were significantly lower at day 5 of co-culture than at day 0.</P><P><B>CONCLUSION</B></P><P>The TCA19 score might be prognostic for target-regimen-specific PFS in stage IV CRC. Down-regulation of SLAMF7 and up-regulation of TREM1 occur in primary and metastatic tumor tissues.</P>

Urinary MicroRNAs of Prostate Cancer: Virus-Encoded hsv1-miRH18 and hsv2-miR-H9-5p Could Be Valuable Diagnostic Markers

Yun, Seok Joong,Jeong, Pildu,Kang, Ho Won,Kim, Ye-Hwan,Kim, Eun-Ah,Yan, Chunri,Choi, Young-Ki,Kim, Dongho,Kim, Jung Min,Kim, Seon-Kyu,Kim, Seon-Young,Kim, Sang Tae,Kim, Won Tae,Lee, Ok-Jun,Koh, Gou-Yo Korean Continence Society 2015 International Neurourology Journal Vol.19 No.2

<P><B>Purpose:</B></P><P>MicroRNAs (miRNAs) in biological fluids are potential biomarkers for the diagnosis and assessment of urological diseases such as benign prostatic hyperplasia (BPH) and prostate cancer (PCa). The aim of the study was to identify and validate urinary cell-free miRNAs that can segregate patients with PCa from those with BPH. </P><P><B>Methods:</B></P><P>In total, 1,052 urine, 150 serum, and 150 prostate tissue samples from patients with PCa or BPH were used in the study. A urine-based miRNA microarray analysis suggested the presence of differentially expressed urinary miRNAs in patients with PCa, and these were further validated in three independent PCa cohorts, using a quantitative reverse transcriptionpolymerase chain reaction analysis. </P><P><B>Results:</B></P><P>The expression levels of hsa-miR-615-3p, hsv1-miR-H18, hsv2-miR-H9-5p, and hsa-miR-4316 were significantly higher in urine samples of patients with PCa than in those of BPH controls. In particular, herpes simplex virus (hsv)-derived hsv1-miR-H18 and hsv2-miR-H9-5p showed better diagnostic performance than did the serum prostate-specific antigen (PSA) test for patients in the PSA gray zone. Furthermore, a combination of urinary hsv2-miR-H9-5p with serum PSA showed high sensitivity and specificity, providing a potential clinical benefit by reducing unnecessary biopsies. </P><P><B>Conclusions:</B></P><P>Our findings showed that hsv-encoded hsv1-miR-H18 and hsv2-miR-H9-5p are significantly associated with PCa and can facilitate early diagnosis of PCa for patients within the serum PSA gray zone.</P>

Spontaneous vaginal hemorrhage caused by choriocarcinoma : Necessity for the early diagnosis and treatment

( Ye Jin Seon ),( Ji Hye Kim ),( Yun Dan Kang ),( Jin Wan Park ),( Jong Soo Kim ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Hemorrhagic choriocarcinoma lesions may lead to life-threatening conditions, but an early diagnosis contributes to a potential cure. We report a case of an woman with choriocarcinoma, that occurred within one month after full term delivery. The 25-year-old patient with gravida 1, para 1 was admitted in our hospital for massive vaginal bleeding and dry cough. Radiological investigation found a 8.4cm mass in enlarged uterus and also multiple nodules in both lungs. Her serum β-hCG level was 747,682 mIU/mL. An emergency surgery was performed to control heavy vaginal bleeding. The patient was early diagnosed as choriocarcinoma FIGO stage III and the score of the Prognostic Scoring Index modified by the WHO was 12. Multiagent chemotherapy, the EMACO regimen(etoposide, actinomycin D, methotrexate, vincristine and cyclophosphamide) was administered. After the seventh chemotherapy session, her serum β-hCG level was <5 mIU/mL. The diagnosis of choriocarcinoma may be difficult, especially in the setting with the limit of medical resources. The early application of various diagnostic techniques such as x-ray, computed tomography is helpful for evaluating the patient´s condition.

Anti-inflammatory Effect of Glucagon Like Peptide-1 Receptor Agonist, Exendin-4, through Modulation of IB1/JIP1 Expression and JNK Signaling in Stroke

Kim, Soojin,Jeong, Jaewon,Jung, Hye-Seon,Kim, Bokyung,Kim, Ye-Eun,Lim, Da-Sol,Kim, So-Dam,Song, Yun Seon The Korean Society for Brain and Neural Science 2017 Experimental Neurobiology Vol.26 No.4

<P>Glucagon like peptide-1 (GLP-1) stimulates glucose-dependent insulin secretion. Dipeptidyl peptidase-4 (DPP-4) inhibitors, which block inactivation of GLP-1, are currently in clinical use for type 2 diabetes mellitus. Recently, GLP-1 has also been reported to have neuroprotective effects in cases of cerebral ischemia. We therefore investigated the neuroprotective effects of GLP-1 receptor (GLP-1R) agonist, exendin-4 (ex-4), after cerebral ischemia-reperfusion injury. Transient middle cerebral artery occlusion (tMCAO) was induced in rats by intracerebroventricular (i.c.v.) administration of ex-4 or ex9-39. Oxygen-glucose deprivation was also induced in primary neurons, bEnd.3 cells, and BV-2. Ischemia-reperfusion injury reduced expression of GLP-1R. Additionally, higher oxidative stress in SOD2 KO mice decreased expression of GLP-1R. Downregulation of GLP-1R by ischemic injury was 70% restored by GLP-1R agonist, ex-4, which resulted in significant reduction of infarct volume. Levels of intracellular cyclic AMP, a second messenger of GLP-1R, were also increased by 2.7-fold as a result of high GLP-1R expression. Moreover, our results showed that ex-4 attenuated pro-inflammatory cyclooxygenase-2 (COX-2) and prostaglandin E<SUB>2</SUB> after MCAO. C-Jun NH<SUB>2</SUB> terminal kinase (JNK) signaling, which stimulates activation of COX-2, was 36% inhibited by i.c.v. injection of ex-4 at 24 h. Islet-brain 1 (IB1), a scaffold regulator of JNK, was 1.7-fold increased by ex-4. GLP-1R activation by ex-4 resulted in reduction of COX-2 through increasing IB1 expression, resulting in anti-inflammatory neuroprotection during stroke. Our study suggests that the anti-inflammatory action of GLP-1 could be used as a new strategy for the treatment of neuroinflammation after stroke accompanied by hyperglycemia.</P>

Enhanced Glycolysis Supports Cell Survival in EGFR-Mutant Lung Adenocarcinoma by Inhibiting Autophagy-Mediated EGFR Degradation

Kim, Ji Hye,Nam, Boas,Choi, Yun Jung,Kim, Seon Ye,Lee, Jung-Eun,Sung, Ki Jung,Kim, Woo Sung,Choi, Chang-Min,Chang, Eun-Ju,Koh, Jae Soo,Song, Joon Seon,Yoon, Shinkyo,Lee, Jae Cheol,Rho, Jin Kyung,Son, American Association for Cancer Research 2018 Cancer Research Vol.78 No.16

<P>Enhanced glycolysis by EGFR mutation is required for maintaining EGFR levels via inhibition of JNK-induced autophagy. This provides a promising rationale for use of JNK activators in patients with EGFR-mutated NSCLC.</P><P>Oncogenic EGFR is essential for the development and growth of non–small cell lung cancer (NSCLC), but the precise roles of EGFR in lung cancer metabolism remain unclear. Here, we show that EGFR mutation-mediated enhancement of glycolysis is critical for EGFR stability. EGFR knockdown significantly decreased levels of glycolytic pathway intermediates via transcriptional regulation of glycolytic genes. EGFR mutation-enhanced glycolysis was required for fueling the tricarboxylic acid cycle, a critical component of EGFR stability. Nonsustained ATP production enhanced reactive oxygen species accumulation and subsequent JNK-mediated activation of autophagy, which in turn induced EGFR degradation. Our data show that EGFR-mutant NSCLCs require EGFR mutation-enhanced glycolysis to maintain EGFR stability. This pathway may serve as an attractive therapeutic target for EGFR-mutant NSCLCs.</P><P><B>Significance:</B> Enhanced glycolysis by EGFR mutation is required for maintaining EGFR levels via inhibition of JNK-induced autophagy. This provides a promising rationale for use of JNK activators in patients with EGFR-mutated NSCLC. <I>Cancer Res; 78(16); 4482–96. ©2018 AACR</I>.</P>

Screening Ethiopian ethnic plants for possible nutritional values to develop RUSF (Ready to Use Supplemental Food)

Ye-Jung Kim,Da-hye Kim,Jung-Ah Kim,Eun-hee Park,In-hyuk Shin,Yihunie A Tegegne,Hee-Seon Kim,Kwangoh Koh 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

Biodegradation of Polyethylene by Pseudomonas alcaligene KY1214 Was Detected through FTIR and SEM Analysis

Ye-Jin KIM,Sol-Hee LEE,Hak-Seon KIM,Hyun-Ouk KIM,Suk-Jin HA 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.4