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Yun, Kwi-Dug,Yang, Yunzhi,Lim, Hyun-Pil,Oh, Gye-Jeong,Koh, Jeong-Tae,Bae, In-Ho,Kim, Jaehyung,Lee, Kwang-Min,Park, Sang-Won Elsevier 2010 Materials science & engineering. C, Materials for Vol.30 No.1
<P><B>Abstract</B></P><P>This study was to evaluate wettability, cell response, and osseointegration of nanotubular titanium (Ti) surface by anodic oxidation. Commercially pure Ti discs were treated by polishing, sandblasting, and anodizing. These surfaces were characterized by scanning electron microscopy and contact angle measurement. MC3T3-E1 osteoblast cell was used to evaluate cell response <I>in vitro</I>. The cell morphology, cell viability, and alkaline phosphatase (ALP) specific activity were assessed. The Ti implants of 2.0mm diameter and 5.0mm long treated by anodizing and sandblasting/anodizing were inserted into the tibia of rats. After 3weeks, the histology of the Ti–bone interface was examined. SEM observations showed that the anodizing and sandblasting/anodizing created the nanotubular surface and graded nanotubular-micro-roughened surfaces, respectively. The anodizing and sandblasting/anodizing significantly improved the hydrophilicity of Ti. The significant greatest cell spreading and ALP specific activity were observed on the graded nanotubular-micro-roughened surfaces treated by sandblasting/anodizing. The <I>in vivo</I> study shows that newly formed bone was intimately in contact with the nanotubular surfaces without adverse immune response. This study has suggested that the graded nanotubular-micro-roughened surface of Ti treated with sandblasting/anodizing is very promising in implantology due to improved hydrophilicity, favorable cell response, and excellent osseointegration.</P>
( Yun Nah Lee ),( Young Seok Kim ),( Sang Gyune Kim ),( Jae Pil Han ),( Sae Hwan Lee ),( Soung Won Jeong ),( Jae Young Jang ),( Hong Soo Kim ),( Boo Sung Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-
Background: Clinical usefulness of noninvasive diagnostic methods for the evaluation of liver fibrosis was not fully documented. We evaluated the accuracy of the FibroScan®, acoustic radiation force impulse (ARFI) and real time elastography (RTE) and the efficacy of the addition of the platelet and P2MS to the elastography for predicting liver fibrosis in nonalcoholic liver disease (NALD). Methods: FibroScan®, ARFI and RTE were performed simultaneously at the same day of liver biopsy in 70 patients with various NALD from October 2010 to March 2011. ARFI was performed with SIEMENS ACUSON S2000 ultrasound system and RTE with HITACHI HI VISION-Preirus and EUP-L52 linear probe (3-7MHz). The median values of 10 measurements in ARFI and RTE were calculated, respectively. We used platelet count and P2/MS [platelet count (109/L)]2/ [monocyte fraction(%)×segmented neutrophil fraction (%)] for predicting more accurately liver fibrosis. We defined Vs as velocity of sheer wave (m/s) in ARFI and Es as elasticity score in RTE. Results: From 70 patients, 10 had METAVIR scoring system F0, 4 had F1, 22 had F2, 12 had F3 and 22 had F4. Positive correlations between each methods and fibrosis were found in FibroScan®, ARFI, RTE, P2MS, Vs/PLT, Vs/P2MS, Es/PLT, and Es/P2MS. In the diagnosis of significanct fibrosis (≥F2), the area under the receiver-operating characteristic curve (AUROC)s by FibroScan, ARFI and RTE are 0.689, 0.687 and 0.554. The AUROCs by Vs/PLT and Es/PLT are 0.860 and 0.769. In addition, the AUROCs by P2MS, Vs/P2MS and Es/P2MS are 0.781, 0.778 and 0.746. Comparing with AUROC of each methods for predicting fibrosis (≥F2), Vs/PLT is superior to ARFI (p=0.0018) and Es/PLT is also superior to RTE (p=0.015). Es/P2MS is superior to RTE (p=0.0316), but Vs/P2MS is not significantly different to ARFI (p=0.1491). Moreover, Vs/P2MS is not superior to Vs/PLT (p=0.0084) and not significantly different to Es/PLT (p=0.5481). Conclusion: Both FibroScan® and ARFI are useful noninvasive modalities for the assessment of liver fibrosis. Vs/P2MS and Es/P2MS can be more helpful for predicting liver fibrosis in NALD than elastography alone, but are not superior to Vs/PLT and Es/PLT.
Yun, Seung Pil,Yoon, Yeo Min,Lee, Jun Hee,Kook, Minjee,Han, Yong-Seok,Jung, Seo Kyung,Lee, Sang Hun MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.2
<P>Mesenchymal stem cells (MSCs) could be a promising solution in the treatment of various diseases including chronic kidney disease (CKD). However, endoplasmic reticulum (ER) stress induced by ischemia in the area of application limits the integration and survival of MSCs in patients. In our study, we generated ER stress-induced conditions in MSCs using <I>P</I>-cresol. As <I>P</I>-cresol is a toxic compound accumulated in the body of CKD patients and induces apoptosis and inflammation through reactive oxygen species (ROS), we observed ER stress-induced MSC apoptosis activated by oxidative stress, which in turn resulted from ROS generation. To overcome stress-induced apoptosis, we investigated the protective effects of tauroursodeoxycholic acid (TUDCA), a bile acid, on ER stress in MSCs. In ER stress, TUDCA treatment of MSCs reduced ER stress-associated protein activation, including GRP78, PERK, eIF2α, ATF4, IRE1α, and CHOP. Next, to explore the protective mechanism adopted by TUDCA, TUDCA-mediated cellular prion protein (PrP<SUP>C</SUP>) activation was assessed. We confirmed that PrP<SUP>C</SUP> expression significantly increased ROS, which was eliminated by superoxide dismutase and catalase in MSCs. These findings suggest that TUDCA protects from inflammation and apoptosis in ER stress via PrP<SUP>C</SUP> expression. Our study demonstrates that TUDCA protects MSCs against inflammation and apoptosis in ER stress by PrP<SUP>C</SUP> expression in response to <I>P</I>-cresol exposure.</P>
Yun, Seung Pil,Han, Yong-Seok,Lee, Jun Hee,Kim, Sang Min,Lee, Sang Hun The Korean Society of Applied Pharmacology 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.4
p-Cresol, found at high concentrations in the serum of chronic kidney failure patients, is known to cause cell senescence and other complications in different parts of the body. p-Cresol is thought to mediate cytotoxic effects through the induction of autophagy response. However, toxic effects of p-cresol on mesenchymal stem cells have not been elucidated. Thus, we aimed to investigate whether p-cresol induces senescence of mesenchymal stem cells, and whether melatonin can ameliorate abnormal autophagy response caused by p-cresol. We found that p-cresol concentration-dependently reduced proliferation of mesenchymal stem cells. Pretreatment with melatonin prevented pro-senescence effects of p-cresol on mesenchymal stem cells. We found that by inducing phosphorylation of Akt and activating the Akt signaling pathway, melatonin enhanced catalase activity and thereby inhibited the accumulation of reactive oxygen species induced by p-cresol in mesenchymal stem cells, ultimately preventing abnormal activation of autophagy. Furthermore, preincubation with melatonin counteracted other pro-senescence changes caused by p-cresol, such as the increase in total 5'-AMP-activated protein kinase expression and decrease in the level of phosphorylated mechanistic target of rapamycin. Ultimately, we discovered that melatonin restored the expression of senescence marker protein 30, which is normally suppressed because of the induction of the autophagy pathway in chronic kidney failure patients by p-cresol. Our findings suggest that stem cell senescence in patients with chronic kidney failure could be potentially rescued by the administration of melatonin, which grants this hormone a novel therapeutic role.
Impact of hs-CRP on outcomes in acute myocardial infarction according to LDL cholesterol level (초)
( Sang Woong Choi ),( Yun Kyeong Cho ),( Jae Pil Lee ),( Ji Hyun Sohn ),( Hyun Ok Cho ),( Hyoung Seob Park ),( Hyuck Jun Yoon ),( Hyung Seop Kim ),( Chang Wook Nam ),( Seung Ho Hur ),( Yoon Nyun Kim ) 대한내과학회 2012 대한내과학회 추계학술대회 Vol.2012 No.1
Yun, Seung Pil,Han, Yong-Seok,Lee, Jun Hee,Yoon, Yeo Min,Yun, Chul Won,Rhee, Peter,Lee, Sang Hun SPANDIDOS PUBLICATIONS 2017 MOLECULAR MEDICINE REPORTS Vol.16 No.5
<P>Cellular prion protein (PrP<SUP>C</SUP>) can replace other pivotal molecules due to its interaction with several partners in performing a variety of important biological functions that may differ between embryonic and mature stem cells. Recent studies have revealed major advances in elucidating the putative role of PrP<SUP>C</SUP> in the regulation of stem cells and its application in stem cell therapy. What is special about PrP<SUP>C</SUP> is that its expression may be regulated by hypoxia-inducible factor (HIF)-1α, which is the transcriptional factor of cellular response to hypoxia. Hypoxic conditions have been known to drive cellular responses that can enhance cell survival, differentiation and angiogenesis through adaptive processes. Our group recently reported hypoxia-enhanced vascular repair of endothelial colony-forming cells on ischemic injury. Hypoxia-induced AKT/signal transducer and activator of transcription 3 phosphorylation eventually increases neovasculogenesis. In stem cell biology, hypoxia promotes the expression of growth factors. According to other studies, aspects of tissue regeneration and cell function are influenced by hypoxia, which serves an essential role in stem cell HIF-1α signaling. All these data suggest the possibility that hypoxia-mediated PrP<SUP>C</SUP> serves an important role in angiogenesis. Therefore, the present review summarizes the characteristics of PrP<SUP>C</SUP>, which is produced by HIF-1α in hypoxia, as it relates to angiogenesis.</P>
Tumebacillus avium sp. nov., isolated from the gut of a cinereous vulture, Aegypius monachus
Sung, Hojun,Kim, Hyun Sik,Lee, June-Young,Kang, Woorim,Kim, Pil Soo,Hyun, Dong-Wook,Tak, Euon Jung,Jung, Mi-Ja,Yun, Ji-Hyun,Kim, Min-Soo,Shin, Na-Ri,Whon, Tae Woong,Rho, Jeong Rae,Park, Sun Duk,Shim, Microbiology Society 2018 International journal of systematic and evolutiona Vol.68 No.5
Long-term follow-up may be needed for pancreaticobiliary reflux in healthy adults
Sung-Pil Yun,Jee Yeon Lee,Hong Jae Jo,Hyun Sung Kim,Dae Hwan Kim,Jae Hun Kim,Sung Jin Park,Do Yoon Park,Hyung-Il Seo 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.2
Purpose: The reflux of pancreatic enzymes into the biliary tract is associated with chronic inflammation and increases cellular proliferation of the biliary epithelium, leading to biliary carcinoma. The aim of this study is to detect the incidence of occult pancreaticobiliary reflux (OPBR) in patients who underwent elective cholecystectomy. Methods: Forty-seven patients with symptomatic gallstones who underwent cholecystectomy were recruited for this study. The gallbladder bile samples were obtained from the specimen of gallbladder and the amylase level was measured. The immunohistochemistry of p53, SMAD4 and Ki-67 were performed for the detection of metaplasia and dysplasia. Results: Biliary amylase was higher than the serum amylase in 10 patients (group A, 15,402.66 ± 33,592.43 IU/L; group B, 13.06 ± 18.12 IU/L). The mean age was 67.2 years in group A and 51.2 in group B (P 〈 0.01). The ratio of male to female was 1:2.3 and 1:1.8 in group A and B, respectively (P = 0.297). Eight patients in group A and thirteen patients in group B had inflammation (P = 0.014). The positive results of the Ki-67 test were exhibited in five cases in each group (P = 0.024). Conclusion: Results from the study indicate that the age was older, degree of inflammation and positive rate of Ki-67 were higher when OPBR was suspected. In conclusion, the patients with OPBR would need long-term follow-up, because the OPBR can cause dysplasia and the reflux of pancreatic juice may be considered as a risk factor for extrahepatic bile duct carcinoma.