http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lee, Young Ju,Ahn, Hyung Joon,Lee, Gi-Ja,Jung, Gyeong Bok,Lee, Gihyun,Kim, Dohyun,Shin, Jae-Ho,Jin, Kyung-Hyun,Park, Hun-Kuk SPIE - International Society for Optical Engineeri 2015 Journal of biomedical optics Vol.20 No.7
<P>The study was to investigate the changes in biochemical properties of activated mature CD8+ T cells related to apoptosis at a molecular level. We confirmed the activation and apoptosis of CD8+ T cells by fluorescence-activated cell sorting and atomic force microscopy and then performed Raman spectral measurements on activated mature CD8+T cells and cellular deoxyribose nucleic acid (DNA). In the activated mature CD8+ T cells, there were increases in protein spectra at 1002 and 1234 cm(-1). In particular, to assess the apoptosis-related DNA spectral signatures, we investigated the spectra of the cellular DNA isolated from resting and activated mature CD8+ T cells. Raman spectra at 765 to 786 cm(-1) and 1053 to 1087 cm(-1) were decreased in activated mature DNA. In addition, we analyzed Raman spectrum using the multivariate statistical method including principal component analysis. Raman spectra of activated mature DNA are especially well-discriminated from those of resting DNA. Our findings regarding the biochemical and structural changes associated with apoptosis in activated mature T cells and cellular DNA according to Raman spectroscopy provide important insights into allospecific immune responses generated after organ transplantation, and may be useful for therapeutic manipulation of the immune response. (C) The Authors. Published by SPIE</P>
Analysis of a head-mounted display-type multifocus display system using a laser scanning method
Kim, Dong-Wook,Kwon, Yong-Moo,Park, Q-Han,Kim, Sung-Kyu SPIE - International Society for Optical Engineeri 2011 Optical engineering Vol.50 No.3
<P>We developed a head-mounted display (HMD)-type multifocus display system using a laser-scanning method to provide an accommodation effect for viewers. This accomplishment indicates that providing a monocular depth cue is possible through this multifocus system. In the system, the optical path is changed by a scanning action. To provide an accurate accommodation effect for the viewer, the multifocus display system is designed and manufactured in accordance with the geometric analysis of the system's scanning action. Using a video camera as a substitute for the viewer, correct focus adjustment without the scanning action problem is demonstrated. By analyzing the scanning action and experimental results, we are able to illustrate the formation of a viewpoint in an HMD-typemultifocus display system using a laser-scanning method. In addition, we demonstrate that the accommodation effect could be provided independent of the viewing condition of the viewer. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3554376]</P>
Enhancement of optical skin clearing efficacy using a microneedle roller
Yoon, Jinhee,Son, Taeyoon,Choi, Eung-ho,Choi, Bernard,Nelson, J. Stuart,Jung, Byungjo SPIE - International Society for Optical Engineeri 2008 Journal of biomedical optics Vol.13 No.2
<P>Light scattering in biological tissues can be reduced by using optical clearing agents. Various physical methods in conjunction with agents have been studied to enhance the optical clearing efficacy of skin for diagnostic and therapeutic applications. In this study, we propose a new physical method to enhance the optical clearing potential of topically applied glycerol. A microneedle roller is used to easily create numerous transdermal microchannels prior to glycerol application. The optical clearing efficacy of skin is quantitatively evaluated with the use of a modulation transfer function target placed underneath ex vivo porcine skin samples. From cross-polarized images acquired at various time points after glycerol application, we find that samples treated with the microneedle roller resulted in an approximately two-fold increase in contrast compared to control samples 30 min after glycerol application. In conclusion, our data suggest that the microneedle roller can be a good physical method to enhance transdermal delivery of optical clearing agents, and hence their optical clearing potential over large regions of skin.</P>
Thermal evaluation of laser exposures in an<i>in vitro</i>retinal model by microthermal sensing
Choi, Tae Y.,Denton, Michael L.,Noojin, Gary D.,Estlack, Larry E.,Shrestha, Ramesh,Rockwell, Benjamin A.,Thomas, Robert,Kim, Dongsik SPIE - International Society for Optical Engineeri 2014 JOURNAL OF BIOMEDICAL OPTICS Vol.19 No.9
<P>A temperature detection system using a micropipette thermocouple sensor was developed for use within mammalian cells during laser exposure with an 8.6-μm beam at 532 nm. We have demonstrated the capability of measuring temperatures at a single-cell level in the microscale range by inserting micropipettebased thermal sensors of size ranging from 2 to 4 μm into the membrane of a live retinal pigment epithelium (RPE) cell subjected to a laser beam. We setup the treatment groups of 532-nm laser-irradiated single RPE cell and in situ temperature recordings were made over time. Thermal profiles are given for representative cells experiencing damage resulting from exposures of 0.2 to 2 s. The measured maximum temperature rise for each cell ranges from 39 to 73C; the RPE cells showed a signature of death for all the cases reported herein. In order to check the cell viability, real-time fluorescence microscopy was used to identify the transition of pigmented RPE cells between viable and damaged states due to laser exposure.</P>
Continuous monitoring of arthritis in animal models using optical imaging modalities
Son, Taeyoon,Yoon, Hyung-Ju,Lee, Saseong,Jang, Won Seuk,Jung, Byungjo,Kim, Wan-Uk SPIE - International Society for Optical Engineeri 2014 JOURNAL OF BIOMEDICAL OPTICS Vol.19 No.10
<P>Given the several difficulties associated with histology, including difficulty in continuous monitoring, this study aimed to investigate the feasibility of optical imaging modalities?cross-polarization color (CPC) imaging, erythema index (EI) imaging, and laser speckle contrast (LSC) imaging?for continuous evaluation and monitoring of arthritis in animal models. C57BL/6 mice, used for the evaluation of arthritis, were divided into three groups: arthritic mice group (AMG), positive control mice group (PCMG), and negative control mice group (NCMG). Complete Freunds adjuvant, mineral oil, and saline were injected into the footpad for AMG, PCMG, and NCMG, respectively. LSC and CPC images were acquired from 0 through 144 h after injection for all groups. EI images were calculated from CPC images. Variations in feet area, EI, and speckle index for each mice group over time were calculated for quantitative evaluation of arthritis. Histological examinations were performed, and the results were found to be consistent with those from optical imaging analysis. Thus, optical imaging modalities may be successfully applied for continuous evaluation and monitoring of arthritis in animal models.</P>