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Roles of cysteine residues in the inhibition of human glutamate dehydrogenase by palmitoyl-CoA
( Hyo Jeong Son ),( Seung Cheol Ha ),( Eun Young Hwang ),( Eun A Kim ),( Jee Yin Ahn ),( Soo Young Choi ),( Sung Woo Cho ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.12
Human glutamate dehydrogenase isozymes (hGDH1 and hGDH2) have been known to be inhibited by palmitoyl-CoA with a high affinity. In this study, we have performed the cassette mutagenesis at six different Cys residues (Cys59, Cys93, Cys119, Cys201, Cys274, and Cys323) to identify palmitoyl- CoA binding sites within hGDH2. Four cysteine residues at positions of C59, C93, C201, or C274 may be involved, at least in part, in the inhibition of hGDH2 by palmitoyl-CoA. There was a biphasic relationship, depending on the levels of palmitoyl-CoA, between the binding of palmitoyl-CoA and the loss of enzyme activity during the inactivation process. The inhibition of hGDH2 by palmitoyl-CoA was not affected by the allosteric inhibitor GTP. Multiple mutagenesis studies on the hGDH2 are in progress to identify the amino acid residues fully responsible for the inhibition by palmitoyl-CoA. [BMB Reports 2012; 45(12): 707-712]
( Sung-woo Ahn ),( Nam-joon Yi ),( Kyung-chul Yoon ),( Hyo-sin Kim ),( Suk-kyun Hong ),( Hyeyoung Kim ),( Jin-young Choi ),( Joon Koo Han ),( Min Uk Kim ),( Hyo-cheol Kim ),( Kwang-woong Lee ),( Kyung 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Pediatric liver transplantation (PLT) has been the key therapy for end stage liver disease and the outcome has been excellent. However, still surgical complication associated with small recipient is the main cause of graft loss. In the present study, we assessed recent advances in outcome of PLTs through our experience. Methods: A total of 237 PLTs performed between Mar 1988 and July 2015 were analyzed. Three chronological periods were investigated: the initial period (1988-2007, n=117), the mid-term period in which our PLT management protocol was settled down (2008-2011, n=61), and the period in which surgical procedures were refined for small children (2012-2015, n=59). Results: The grafts’ (84% vs. 82% vs. 98%) and patients’ (84% vs. 87% vs. 98%) survival have been improved (p<0.05), whereas the proportion of Child class C (12% vs. 21% vs. 34%), the proportion of deceased donor (25% vs. 41% vs. 54%), and split PLT (15%vs. 20% vs. 39%) increased (p<0.05). The incidence of surgical complication has been improved, especially in hepatic artery (4% vs. 12% vs. 0%) but there was no significance. ABO incompatible PLT has introduced on the last period (9%). Conclusions: The quality of the PLT has recently been standardized through a large volume of experience, and the operation has been proven to improve the survival outcome. However, a constant evaluation of our experience is critical for further progress.
Intra-operative Neurological Monitoring and Anesthesia
( Sang-ku Park ),( Sung-hyuk Lim ),( Chan-woo Park ),( Jin-woo Park ),( Dong-jun Kim ),( Ji-hyuk Kang ),( Hyo-geun Jee ),( Gi-bong Kim ) 대한임상검사과학회 2012 대한임상검사과학회지(KJCLS) Vol.44 No.4
The purpose of intra-operative neurological monitoring (INM) is to minimize surgically induced nerve damage, sensory nerves and motor neurons without affecting the operations to proceed during surgery such as evoked potentials (EP), electromyography (EMG), electroencephalography (EEG), transcranial doppler (TCD), etc. During the course of checking a patient’s condition, surveillance of ambulatory patients is a very different thing to check if the test is done under general anesthesia. INM can be possible or impossible depending on the type of drugs used and their concentrations because the monitoring is performed under anesthesia. Therefore, it is emphasized on the necessity of reviewing anesthesia which influences on INM.