울증(鬱證)환자에게 용서프로그램을 활용한 오지상승요법(五志相勝療法)을 시행하여 호전된 1례

이상언 ( Sang Eon Lee ),노동진 ( Dong Jin No ),박장호 ( Jang Ho Park ),이고은 ( Go Eun Lee ),박인숙 ( In Sook Park ),류영수 ( Yeong Su Lyu ),안민섭 ( Min Seob Ahn ),정지호 ( Ji Ho Jung ) 대한한방신경정신과학회 2010 동의신경정신과학회지 Vol.21 No.2

Yuzheng(鬱證) comes from obstruction of qi by stress. The patient has depressed mood, irritable sign, chest discomfort, costal pain, angry state or some strange feeling on the throat. Oh-Ji-Sang-Seung(五志相勝) therapy is base on the theory of interrelation in five elements in oriental medicine. The contents of Oh-Ji-Sang-Seung(五志相勝) therapy include five subjugations of five emotions. Anxiety subjugates fear(思勝恐), fear subjugates joy(恐勝喜), joy subjugates pity(喜勝悲), pity subjugates anger(悲勝怒), and anger subjugates anxiety(怒勝思). Forgiveness program is a kind of psychological therapies to decrease the degree of anger and it is included in Oh-Ji-Sang-Seung(五志相勝) therapy. In this case, a female patient, 50 years old, who suffered from Yuzheng(鬱證) with chest discomfort, irritable sign, easily angry state, depressed mood, hot flush, insomnia. We used Oh-Ji-Sang-Seung(五志相勝) therapy besides herbal medication, acupuncture to her condition got improved. Therefore we reported it for the treatment.

Induced Magnetic Anisotropy in Permalloy Films Annealed with Magnetic Field

Eon Byeong Park,Yong-Chan Kim,Sung-Uk Jang,Ji-Hong Kim,Sang-Wook Han,권순주 대한금속·재료학회 2013 METALS AND MATERIALS International Vol.19 No.1

The effect of magnetic annealing on Fe21Ni79 films was investigated through the characterization of crystal structure and magnetic properties. Fe21Ni79 films were deposited on thermally oxidized Si substrates using a DC magnetron sputter. A highly a-axis oriented Fe21Ni79 film was grown on Si substrate by magnetic annealing. Columnar grain growth was dominant in the films annealed with magnetic field. Both the area surrounded by hysteresis curve, and coercivity, were increased by magnetic annealing, indicating that the magnetic annealing induced the anisotropy energy. Through EXAFS analysis, 1.19×10.3 was obtained as the strain of a specimen annealed with magnetic field. This value confirmed that induced magnetic anisotropy was formed by atomic pair ordering.

Mepivacaine-induced contraction is attenuated by endothelial nitric oxide release in isolated rat aorta

Sung, Hui-Jin,Choi, Mun-Jeoung,Ok, Seong-Ho,Lee, Soo Hee,Hwang, Il Jeong,Kim, Hee Sook,Chang, Ki Churl,Shin, Il-Woo,Lee, Heon-Keun,Park, Kyeong-Eon,Chung, Young-Kyun,Sohn, Ju-Tae Canadian Science Publishing 2012 Canadian journal of physiology and pharmacology Vol.90 No.7

<P> Mepivacaine is an aminoamide-linked local anesthetic with an intermediate duration that intrinsically produces vasoconstriction both in vivo and in vitro. The aims of this in-vitro study were to examine the direct effect of mepivacaine in isolated rat aortic rings and to determine the associated cellular mechanism with a particular focus on endothelium-derived vasodilators, which modulate vascular tone. In the aortic rings with or without endothelium, cumulative mepivacaine concentration-response curves were generated in the presence or absence of the following antagonists: N<SUP>ω</SUP>-nitro-l-arginine methyl ester [l-NAME], indomethacin, fluconazole, methylene blue, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one [ODQ], verapamil, and calcium-free Krebs solution. Mepivacaine produced vasoconstriction at low concentrations (1 × 10<SUP>−3</SUP> and 3 × 10<SUP>−3</SUP> mol/L) followed by vasodilation at a high concentration (1 × 10<SUP>−2</SUP> mol/L). The mepivacaine-induced contraction was higher in endothelium-denuded aortae than in endothelium-intact aortae. Pretreatment with l-NAME, ODQ, and methylene blue enhanced mepivacaine-induced contraction in the endothelium-intact rings, whereas fluconazole had no effect. Indomethacin slightly attenuated mepivacaine-induced contraction, whereas verapamil and calcium-free Krebs solution more strongly attenuated this contraction. The vasoconstriction induced by mepivacaine is attenuated mainly by the endothelial nitric oxide - cyclic guanosine monophosphate pathway. In addition, mepivacaine-induced contraction involves cyclooxygenase pathway activation and extracellular calcium influx via voltage-operated calcium channels. </P>

Discovery of Novel DUSP16 Phosphatase Inhibitors through Virtual Screening with Homology Modeled Protein Structure

Park, Hwangseo,Park, So Ya,Nam, Sang-Won,Ryu, Seong Eon SAGE Publications 2014 Journal of biomolecular screening Vol.19 No.10

<P>Recently, dual-specificity phosphatase 16 (DUSP16) emerged as a promising therapeutic target protein for the development of anti-atherosclerosis and anticancer medicines. The present study was undertaken to identify the novel inhibitors of DUSP16 based on the structure-based virtual screening. We have been able to find seven novel inhibitors of DUSP16 through the drug design protocol involving homology modeling of the target protein, docking simulations between DUSP16 and its putative inhibitors with the modified scoring function, and in vitro enzyme assay. These inhibitors revealed good potency, with IC<SUB>50</SUB> values ranging from 1 to 22 µM, and they were also screened computationally for having desirable physicochemical properties as drug candidates. Therefore, they deserve consideration for further development by structure-activity relationship studies to optimize the inhibitory activity against DUSP16. Structural features relevant to the stabilization of the newly identified inhibitors in the active site of DUSP16 are addressed in detail.</P>

Culture, Ambidexterity, and Performance

Sang-Eon Park(박상언),Mooweon Rhee(이무원),Daegyu Yang(양대규) 한국인적자원개발학회 2017 인적자원개발연구 Vol.20 No.1

Discovery of Novel DUSP4 Inhibitors through the Virtual Screening with Docking Simulations

Park, Hwangseo,Jeon, Tae Jin,Chien, Pham Ngoc,Park, So Ya,Oh, Sung Min,Kim, Seung Jun,Ryu, Seong Eon Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.9

Dual specificity protein phosphatase 4 (DUSP4) has been considered a promising target for the development of therapeutics for various human cancers. Here, we report the first example for a successful application of the structure-based virtual screening to identify the novel small-molecule DUSP4 inhibitors. As a consequence of the virtual screening with the modified scoring function to include an effective molecular solvation free energy term, five micromolar DUSP4 inhibitors are found with the associated $IC_{50}$ values ranging from 3.5 to $10.8{\mu}M$. Because these newly identified inhibitors were also screened for having desirable physicochemical properties as a drug candidate, they may serve as a starting point of the structure-activity relationship study to optimize the medical efficacy. Structural features relevant to the stabilization of the new inhibitors in the active site of DUSP4 are discussed in detail.

Dual control of the vestibulosympathetic reflex following hypotension in rats

Sang Eon Park,Yuan-Zhe Jin,Byung Rim Park 대한생리학회-대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.13 No.3

Orthostatic hypotension (OH) is associated with symptoms including headache, dizziness, and syncope. The incidence of OH increases with age. Attenuation of the vestibulosympathetic reflex (VSR) is also associated with an increased incidence of OH. In order to understand the pathophysiology of OH, we investigated the physiological characteristics of the VSR in the disorder. We applied sodium nitroprusside (SNP) to conscious rats with sinoaortic denervation in order to induce hypotension. Expression of pERK in the intermediolateral cell column (IMC) of the T4~7 thoracic spinal regions, blood epinephrine levels, and blood pressure were evaluated following the administration of glutamate and/or SNP. SNP-induced hypotension led to increased pERK expression in the medial vestibular nucleus (MVN), rostral ventrolateral medullary nucleus (RVLM) and the IMC, as well as increased blood epinephrine levels. We co-administered either a glutamate receptor agonist or a glutamate receptor antagonist to the MVN or the RVLM. The administration of the glutamate receptor agonists, AMPA or NMDA, to the MVN or RVLM led to elevated blood pressure, increased pERK expression in the IMC, and increased blood epinephrine levels. Administration of the glutamate receptor antagonists, CNQX or MK801, to the MVN or RVLM attenuated the increased pERK expression and blood epinephrine levels caused by SNP-induced hypotension. These results suggest that two components of the pathway which maintains blood pressure are involved in the VSR induced by SNP. These are the neurogenic control of blood pressure via the RVLM and the humoral control of blood pressure via epinephrine release from the adrenal medulla.

Risk Factors Predicting Unfavorable Neurological Outcome during the Early Period after Traumatic Brain Injury

Park, Jung-Eon,Kim, Sang-Hyun,Yoon, Soo-Han,Cho, Kyung-Gi,Kim, Se-Hyuk The Korean Neurosurgical Society 2009 Journal of Korean neurosurgical society Vol.45 No.2

Objective : We aimed to identify clinico-radiological risk factors that may predict unfavorable neurological outcomes in traumatic brain injury (TBI), and to establish a guideline for patient selection in clinical trials that would improve neurological outcome during the early post TBI period. Methods : Initial clinico-radiological data of 115 TBI patients were collected prospectively. Regular neurological assessment after standard treatment divided the above patients into 2 groups after 6 months : the Favorable neurological outcome group (GOS : good & moderate disability, DRS : 0-6, LCFS : 8-10) and the Unfavorable group (GOS : severe disability-death, DRS : 7-29 and death, LCFS : 1-7 and death). Results : There was a higher incidence of age $\geq$35 years, low initial GCS score, at least unilateral pupil dilatation, and neurological deficit in the Unfavorable group. The presence of bilateral parenchymal lesions or lesions involving the midline structures in the initial brain CT was observed to be a radiological risk factor for unfavorable outcome. Multivariate analysis demonstrated that age and initial GCS score were independent risk factors. The majority of the Favorable group patients with at least one or more risk factors showed improvement of GCS scores within 2 months after TBI. Conclusion : Patients with the above mentioned clinico-radiological risk factors who received standard treatment, but did not demonstrate neurological improvement within 2 months after TBI were deemed at risk for unfavorable outcome. These patients may be eligible candidates for clinical trials that would improve functional outcome after TBI.

Mackerel-derived fermented fish oil protects skin against UVB-induced cellular damage by inhibiting oxidative stress

Park, Jeong Eon,Hyun, Yu Jae,Piao, Mei Jing,Kang, Kyoung Ah,Ryu, Yea Seong,Shilnikova, Kristina,Zhen, Ao Xuan,Ahn, Mee Jung,Ahn, Yong Seok,Koh, Young Sang,Kang, Hee Kyoung,Hyun, Jin Won Elsevier 2018 Journal of Functional Foods Vol.46 No.-

<P><B>Abstract</B></P> <P>This study investigated the protective effect of mackerel-derived fermented fish oil (FFO) against UVB radiation-induced oxidative stress in human HaCaT keratinocytes and mouse skin tissue. FFO treatment scavenged UVB-induced intracellular reactive oxygen species and attenuated oxidative modifications including lipid peroxidation, protein carbonylation, and DNA damage. FFO treatment reduced UVB-induced apoptosis by reducing DNA fragmentation, caspase activation, and proapoptotic protein expression. UVB radiation activated phospho-extracellular signal-regulated kinase, phospho-c-Jun N-terminal kinase, and phospho-p38, whereas their specific inhibitors with FFO treatment abrogated the cell viability and apoptosis increased by UVB irradiation. FFO was more cytoprotective than docosahexaenoic acid, the main component of fish oil, against UVB exposure. Furthermore, the cytoprotective effect of FFO was evident in both UVB-exposed HaCaT cell and mouse models. Overall, these results demonstrate that FFO protects the skin against UVB-induced oxidative stress through antioxidant effects. FFO has the potential for development as a functional food against UVB-induced skin damage.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mackerel-derived fermented fish oil (FFO) scavenged UVB-induced intracellular reactive oxygen species. </LI> <LI> FFO treatment attenuated UVB-induced oxidative skin cellular modifications and apoptotic cell death. </LI> <LI> FFO may be developed as a functional food against UVB-induced skin damage. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>FFO prevented epidermal damage including oxidative cellular damage and apoptotic cell death by directly inhibiting UVB radiation or decreasing ROS level induced by UVB radiation.</P> <P>[DISPLAY OMISSION]</P>

CO₂ Use as Oxidant for Vallue-added Basic Chemicals

Sang-Eon Park,Changbum Jo 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-