PCR-SSCP of Serum Lysozyme Gene (Exon-III) in Riverine Buffalo and Its Association with Lysozyme Activity and Somatic Cell Count

Sahoo, Nihar Ranjan,Kumar, Pushpendra,Bhushan, Bharat,Bhattacharya, T.K.,Sharma, Arjava,Dayal, Sanker,Pankaj, Prabhat Kumar,Sahoo, Monalisa Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.8

Serum lysozyme gene is one of the important genes influencing the immune system as its product can cause lysis of bacterial cell wall by cleaving the peptidoglycan layer. The present investigation on the serum lysozyme gene of Indian riverine buffalo was undertaken with the objectives to identify and characterize single nucleotide polymorphic patterns by PCR-SSCP method as well as to study the effect of different genotypes on serum lysozyme activity and somatic cell count. A total of 280 animals comprising four different famous bubaline breeds (Murrah, Mehsana, Surti and Bhadawari), spread over six different farms across the country were used for this study. A 276 bp (partial intron 2, complete exon 3 and partial intron 3) fragment of lysozyme gene was screened for polymorphism using the SSCP technique. Four genotypes namely AA, AB, BC and AC were observed, out of which BC genotype was found to be the most frequent. Among these three alleles, C allele (0.38) was most prevalent in these populations. Various SSCP allelic variants were cloned for sequencing and sequences were submitted to NCBI Genbank. From the alignment of the nucleotide sequences of various allelic variants, it was found that there were differences in 12 positions among the alleles, out of which maximum variation (at 8 places) was found in the intronic region. The allele A was closer to allele-C than allele-B. Allele B was phylogenetically equidistant from both of the other alleles. Mean lysozyme activity determined in serum samples of different animals of Murrah buffalo was $27.35{\pm}2.42\;{\mu}g$ per ml of serum, whereas the mean somatic cell count was $1.25{\pm}0.13{\times}10^5$ cells per ml of milk. The SSCP pattern-wise effects of various genotypes on lysozyme activity and SCC were analyzed. Although the mean values were apparently different in various genotypes, these differences were statistically non-significant. It can be concluded that the riverine buffaloes are sufficiently polymorphic with respect to serum lysozyme gene. The absence of AA genotype in Bhadawari breed of buffalo can be considered as a marker for breed characterization. The difference of four nucleotides in exon-3 indicates high selection pressure on the gene.

Preparation of ultra-porous graphene oxide using a glucose-mediated hydrothermal method for efficient removal of fluoride ions from water: kinetics, isotherms and co-existing ions studies

Sahoo Shraban Kumar,Sahoo Jitendra Kumar,Biswal Susanta Kumar,Gagan Kumar Panigrahi 한국탄소학회 2024 Carbon Letters Vol.34 No.1

Porous graphene oxide (P-GO) was successfully synthesized by using a simple glucose mediated hydrothermal method form prepared graphene oxide (GO). Then the P-GO was characterized by X-ray Powder Diffraction (XRD), Fourier-Transform Infrared (FITR), Raman, Brunauer–Emmett–Teller (BET), Field Emission Scanning Electron Microscopy (FESEM) and Transmission Electron Microscopy (TEM) analysis to determine the crystallinity, surface functionality, surface defect, surface area and porous nature of the material. For the comparative properties studies with P-GO, the synthesised GO was also characterised using the aforementioned analytical techniques. The formation of macroporous 2D sheet-like structure of P-GO with pore size diameters of 0.2–0.5 µm was confirmed by FESEM and TEM images. The surface area of P-GO was found to be 1272 m2/g which is much higher compare to GO (i.e., 172 m2/g) because of porous structure. P-GO was used for the adsorptive removal of F− ions from water using batch adsorption method. The highest adsorption occurs in the pH range of 5–7 with maximum adsorption capacity of 1272 mg/g. The experimental data revealed that the adsorption process obeys Langmuir monolayer isotherm model. The kinetic analysis revealed that the adsorption procedure is extremely rapid and mainly fit to the Pseudo-second-order (PSO) model. The effect of co-existing ions on fluoride adsorption capacity by P-GO decreases in the following order: PO43− > CO32− > SO42− > HCO3− > NO3− > Cl−. The mechanism of adsorption of fluoride onto the P-GO surface includes electrostatic interactions and hydrogen bonding.

A Critical Survey of Seaweed Diversity of Chilika Lake, India

Sahoo, Debasish,Sahu, Nivedita,Sahoo, Dinabandhu The Korean Society of Phycology 2003 ALGAE Vol.18 No.1

Numerical buckling temperature prediction of graded sandwich panel using higher order shear deformation theory under variable temperature loading

Brundaban Sahoo,Bamadev Sahoo,Nitin Sharma,Kulmani Mehar,Subrata Kumar Panda 국제구조공학회 2020 Smart Structures and Systems, An International Jou Vol.26 No.5

The finite element solutions of thermal buckling load values of the graded sandwich curved shell structure are reported in this research using a higher-order kinematic model including the shear deformation effect. The numerical buckling temperature has been computed using an in-house specialized code (MATLAB environment) prepared in the framework of the current mathematical formulation. In addition, the mathematical model includes the excess structural distortion under the influence of elevated environment via Green-Lagrange nonlinear strain. The corresponding eigenvalue equation has been solved to predict the critical buckling temperature of the graded sandwich structure. The numerical stability and the accuracy of the current solution have been confirmed by comparing with the available published results. Thereafter, the model is extended to bring out the influences of structural parameters i.e. the curvature ratio, core-face thickness ratio, support conditions, power-law indices and sandwich types on the thermal buckling behavior of graded sandwich curved shell panels.

Thermal frequency analysis of FG sandwich structure under variable temperature loading

Brundaban Sahoo,Kulmani Mehar,Bamadev Sahoo,Nitin Sharma,Subrata Kumar Panda 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.77 No.1

The thermal eigenvalue responses of the graded sandwich shell structure are evaluated numerically under the variable thermal loadings considering the temperature-dependent properties. The polynomial type rule-based sandwich panel model is derived using higher-order type kinematics considering the shear deformation in the framework of the equivalent single-layer theory. The frequency values are computed through an own home-made computer code (MATLAB environment) prepared using the finite element type higher-order formulation. The sandwich face-sheets and the metal core are discretized via isoparametric quadrilateral Lagrangian element. The model convergence is checked by solving the similar type published numerical examples in the open domain and extended for the comparison of natural frequencies to have the final confirmation of the model accuracy. Also, the influence of each variable structural parameter, i.e. the curvature ratios, core-face thickness ratios, end-support conditions, the power-law indices and sandwich types (symmetrical and unsymmetrical) on the thermal frequencies of FG sandwich curved shell panel model. The solutions are helping to bring out the necessary influence of one or more parameters on the frequencies. The effects of individual and the combined parameters as well as the temperature profiles (uniform, linear and nonlinear) are examined through several numerical examples, which affect the structural strength/stiffness values. The present study may help in designing the future graded structures which are under the influence of the variable temperature loading.

Problems and solutions to conduct of thesis of postgraduate medical students during the COVID-19 pandemic: an insight into the students perspective

Alok Kumar Sahoo,Nitasha Mishra,Manisha Sahoo,Premangshu Ghoshal 한국의학교육학회 2023 Korean journal of medical education Vol.35 No.1

Purpose: The coronavirus disease 2019 (COVID-19) pandemic has adversely impacted medical education worldwide. However, its impact on the postgraduate medical thesis and dissertation work is still not evaluated. Through this study, we planned to find out the problems brought by the pandemic and likely alternatives and possible solutions to thrust the academic competence of postgraduate students. Methods: After obtaining institutional ethics committee approval, we sent a 13-item questionnaire to postgraduate medical students in India via various social media online platforms. Data on the impact of the COVID-19 pandemic on thesis work and alternatives/solutions to improve the research competence were collected on a Likert scale and analyzed. Results: We received a total of 398 responses out of which 377 entries were included for final analysis. The majority of participants (88%) reportedly had an adverse impact on the thesis work and out of 25% of the participants who recently submitted their around 45% had to do so without achieving the estimated sample size. The 6-month departmental review for thesis progress was seen in merely 28% of participants. Possible alternatives suggested were the maintenance of log books, task-based assessment of research methodology, departmental audits, and systematic reviews. Solutions suggested for improving the research competence of students were a compulsory research methodology curriculum, a biostatistics department in each institution, permission to conduct thesis work beyond submission time, exclusive time for research work, and financial incentives. Conclusion: Modification in the research aspect of the current postgraduate medical education is the need of the hour and the pandemic has enlightened us regarding the current weaknesses.

Redox-Driven Route for Widening Voltage Window in Asymmetric Supercapacitor

Sahoo, Ramkrishna,Pham, Duy Tho,Lee, Tae Hoon,Luu, Thi Hoai Thuong,Seok, Jinbong,Lee, Young Hee American Chemical Society 2018 ACS NANO Vol.12 No.8

<P>Although aqueous asymmetric supercapacitors are promising technologies because of their high-energy density and enhanced safety, their voltage window is still limited by the narrow stability window of water. Redox reactions at suitable electrodes near the water splitting potential can increase the working potential. Here, we demonstrate a kinetic approach for expanding the voltage window of aqueous asymmetric supercapacitors using <I>in situ</I> activated Mn<SUB>3</SUB>O<SUB>4</SUB> and VO<SUB>2</SUB> electrodes. The underlying mechanism indicates a specific potential of ∼1 V <I>vs</I> Ag/AgCl for the oxidation of Mn<SUP>4+</SUP>-to-Mn<SUP>7+</SUP> at the positive electrode and ∼ -0.8 V <I>vs</I> Ag/AgCl for the reduction of V<SUP>3+</SUP>-to-V<SUP>2+</SUP> at the negative electrode, which limits oxygen and hydrogen evolution reactions, respectively. The as-fabricated aqueous asymmetric supercapacitor exhibited a working voltage of 2.2 V with a high-energy density of 42.7 Wh/kg and a power density of ∼1.1 kW/kg. This mechanism improves the voltage window and energy and power densities.</P> [FIG OMISSION]</BR>

Potentially Multidentate Tripodal Amine Catechol Ligands as Chelators for Ga(III) and In(III)

Sahoo, Suban K.,Baral, Minati,Bera, Rati Kanta,Kanungo, B. K. Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.9

The binding abilities of two multidentate tripodal amine catechol ligands, cis,cis-1,3,5-tris[(2,3-dihydroxybenzylamino) aminomethyl]cyclohexane (TMACHCAT, $L^1)\;and\;N^1,N^3,N^5$-tris(2-(2,3-dihydroxybenzylamino) ethyl)cyclohexane-1,3,5-tricarboxamide (CYCOENCAT, $L^2$) with Ga(III) and In(III) have been investigated by potentiometric and spectrophotometric methods in an aqueous medium of 0.1 M KCl at 25 ${\pm}\;1\;{^{\circ}C}.$ The ligands $L^1\;and\;L^2$ formed various monomeric species $MLH_3,\;MLH_2$, MLH and ML (M = $Ga^{+3}\;and\;In^{+3}$) and showed potential to form strong encapsulated tris(catechol) type complexes. The coordination modes, binding ability and selectivity of the ligands towards Ga(III) and In(III) have been discussed with the help of experimental evidences, and supported with molecular modeling calculations.

Dietary Protein Restriction on Growth and Immuno-biochemical Response of Crossbred Calves during Post-ruminant Phase of Life

Sahoo, A.,Mishra, S.C.,Pathak, N.N. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.8

Sixteen crossbred (Bos indicus${\times}$Bos taurus) calves were randomly distributed in two groups (NP and LP) of eight calves each to study the effect of restricted (75%) protein supply on growth and immuno-biochemical response as an indicator of production and health of under-nourished animals during 3 to 9 months of age. The normal requirement of protein was provided to group NP and a less of 25% to group LP through calculated amount of concentrate and roughage in their daily ration. Assessment was made for weekly change in live weight, periodic alteration in blood metabolites and immunological status at six months of age in calves. An initial (during 3 to 6 months of age) depression (p<0.05) in growth was seen in low protein fed group (LP) compared to NP, which became non-significant in the later period of life (6 to 9 months of age). There was no significant effect on haemoglobin, total protein, albumin and globulin concentration except that of urea, which was decreased significantly (p<0.05) in animals fed on low protein diet ($19.83{\pm}1.25$ vs $25.93{\pm}1.29mg/dl$). The treatment effect that was seen in different periods of life was not uniform for other parameters except for urea, which showed a regular depression in LP compared to NP. The assessment of immunological status by indirect haemagglutination (IHA) test against Pasteurella multocida (P52 strain) was considerably (p<0.05) reduced in animals on LP ration compared to those on NP. It is thus argued that with poor nutrition (low protein) and state of compromised immunological response the production and health of the animals will be adversely affected.

An Empirical Comparison of Ratio and PPS Strategies

Sahoo, L.N.,Dalabehera, M. The Korean Statistical Society 2002 Journal of the Korean Statistical Society Vol.31 No.2

In an effort to make a right choice among ratio estimation strategies and PPS sampling strategies, we conduct an empirical investigation of the relative performances of three ratio estimation strategies and four PPS estimation strategies using a set of 12 natural populations. The quality of a strategy is measured in the traditional way, namely with the consideration of efficiency, achieved coverage rate of the nominal 99% confidence interval and approach to normality (asymmetry).