대두박 수준을 달리한 사료내 미생물 PHYTASE 의 첨가가 잉어의 성장 및 인 배설량에 미치는 영향

김광석(K . S . Kim),김정대(J . D . Kim),송재성(J . S . Song),정관식(K . S . Jeong),우영배(Y . B . Woo) 한국영양사료학회 1996 韓國營養飼料學會誌 Vol.20 No.5

Quantifying herbicide dose-response and resistance in <i>Echinochloa</i> spp. by measuring root length in growth pouches

Zhang, C. J.,Lim, S. H.,Kim, J. W.,Song, J. S.,Yook, M. J.,Nah, G.,Valverde, B. E.,Kim, D. S. Canadian Science Publishing 2015 Canadian journal of plant science. Revue canadienn Vol.95 No.6

<P> Zhang, C. J., Lim, S. H., Kim, J. W., Song, J. S., Yook, M. J., Nah, G., Valverde, N. E. and Kim, D. S. 2015. Quantifying herbicide dose-response and resistance in Echinochloa spp. by measuring root length in growth pouches. Can. J. Plant Sci. 95: 1181-1192. The aim of the presented study was to develop a bioassay for rapid diagnosis of herbicide dose-response and resistance in Echinochloa. Pre-germinated seeds of Echinochloa spp. were incubated in growth pouches (18 cm×16.5 cm) containing herbicide solutions in a range of concentrations. Shoot and root lengths were measured after 6 d of incubation. Dose-responses estimated by measuring root lengths in the growth pouches were well-described by the log-logistic dose-response model and similar to those estimated by a whole-plant assay. Accurate dose-response curves were successfully generated for several herbicides with different modes of action, suggesting that the growth pouch method can be used for herbicide bioassays. The suitability of the growth pouch method for rapid diagnosis of acetyl coenzyme-A carboxylase (ACCase) and acetolactate synthase (ALS) inhibitor resistance in Echinochloa spp. was also tested. For cyhalofop-butyl, resistant and susceptible biotypes were discriminated at 180-300 mg a.i. L<SUP>−1</SUP> and 80-120 mg a.i. L<SUP>−1</SUP> for barnyardgrass (E. crus-galli) and late watergrass (E. oryzicola), respectively. For penoxsulam, the discriminatory dosage was 350-500 mg a.i. L<SUP>−1</SUP> for barnyardgrass and 650-1000 mg a.i. L<SUP>−1</SUP> for late watergrass. The method was further used to identify late watergrass biotypes resistant and susceptible to two other ALS inhibitors, azimsulfuron and bispyribac-sodium. Our results show that the growth pouch method can be reliably used in herbicide dose-response studies and to diagnose herbicide resistance in Echinochloa spp., with significant time and cost savings compared with conventional whole-plant assays. </P>

Evaluation of atmospheric pressure plasma to improve the safety of sliced cheese and ham inoculated by 3-strain cocktail Listeria monocytogenes

Song, H.P.,Kim, B.,Choe, J.H.,Jung, S.,Moon, S.Y.,Choe, W.,Jo, C. Academic Press 2009 FOOD MICROBIOLOGY Vol.26 No.4

The objective of this study was to evaluate the efficacy of atmospheric pressure plasma (APP), which is capable of operating at atmospheric pressure in air, in sliced cheese and ham inoculated by 3-strain cocktail of Listeria monocytogenes (ATCC 19114, 19115, and 19111, LMC). The process parameters considered were input power (75, 100, 125, and 150 W) and plasma exposure time (60, 90, and 120 s). Microbial log reduction increased with increases of input power and plasma exposure time. After 120 s APP treatments at 75, 100, and 125 W, the viable cells of LMC were reduced by 1.70, 2.78, and 5.82 log in sliced cheese, respectively. More than 8 log reductions can be achieved in 120 s at 150 W. In contrast, reductions after 120 s ranged from 0.25 to 1.73 log CFU/g in sliced ham. Calculated D values, the exposure time required to inactivate 90% of a population, from the survival curves of 75, 100, 125, and 150 W of APP treatments were 71.43, 62.50, 19.65, and 17.27 s for LMC in sliced cheese, respectively, and those in sliced ham were 476.19, 87.72, 70.92, and 63.69 s. No viable cells were detected at 125 and 150 W of APP treatment in sliced cheese, irrespective of plasma exposure time, after 1 week at a detection limit of 10<SUP>1</SUP> CFU/g. These results indicate that the inactivation effects of APP on L. monocytogenes are strongly dependent on the type of food.

복합재료 특성 평가 1 : 다축 구조 S-2 유리섬유 복합재의 충격 특성

송승욱 ( S. W. Song ),이창훈 ( C. H. Lee ),변준형 ( J. H. Byun ),황병선 ( B. S. Hwang ),엄문광 ( M. K. Um ),이상관 ( S. K. Lee ) 한국복합재료학회 2005 한국복합재료학회 학술발표대회 논문집 Vol.- No.1

ZNF509S1 downregulates PUMA by inhibiting p53K382 acetylation and p53-DNA binding

Jeon, B.N.,Yoon, J.H.,Han, D.,Kim, M.K.,Kim, Y.,Choi, S.H.,Song, J.,Kim, K.S.,Kim, K.,Hur, M.W. Elsevier Science 2017 Biochimica et biophysica acta. Gene regulatory mec Vol.1860 No.9

Expression of the POK family protein ZNF509L, and -its S1 isoform, is induced by p53 upon exposure to genotoxic stress. Due to alternative splicing of the ZNF509 primary transcript, ZNF509S1 lacks the 6 zinc-fingers and C-terminus of ZNF509L, resulting in only one zinc-finger. ZNF509L and -S1 inhibit cell proliferation by activating p21/CDKN1A and RB transcription, respectively. When cells are exposed to severe DNA damage, p53 activates PUMA (p53-upregulated modulator of apoptosis) transcription. Interestingly, apoptosis due to transcriptional activation of PUMA by p53 is attenuated by ZNF509S1. Thus we investigated the molecular mechanism(s) underlying the transcriptional attenuation and anti-apoptotic effects of ZNF509S1. We show that ZNF509S1 modulation of p53 activity is important in PUMA gene transcription by modulating post-translational modification of p53 by p300. ZNF509S1 directly interacts with p53 and inhibits p300-mediated acetylation of p53 lysine K382, with deacetylation of p53 K382 leading to decreased DNA binding at the p53 response element 1 of the PUMA promoter. ZNF509S1 may play a role not only in cell cycle arrest, by activating RB expression, but also in rescuing cells from apoptotic death by repressing PUMA expression in cells exposed to severe DNA damage.

Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration

Song, D‐,S.,Park, J‐,C.,Jung, I‐,H.,Choi, S‐,H.,Cho, K‐,S.,Kim, C‐,K.,Kim, C‐,S. Blackwell Publishing Ltd 2011 Journal of periodontal research Vol.46 No.2

<P> <I>Song D‐S, Park J‐C, Jung I‐H, Choi S‐H, Cho K‐S, Kim C‐K, Kim C‐S. Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration. J Periodont Res 2011; 46: 193–203. © 2010 John Wiley & Sons A/S</I> </P><P><B>Background and Objective: </B> Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) is a potent inducer for the regeneration of mineralized tissue, but has a limited effect on the regeneration of cementum and periodontal ligament (PDL). The aim of the present study was to determine the effects of rhBMP‐2 on the <I>in vitro</I> and <I>in vivo</I> biologic activity of well‐characterized human PDL stem cells (hPDLSCs) and to elucidate the underlying mechanism of minimal periodontal regeneration by rhBMP‐2.</P><P><B>Material and Methods: </B> hPDLSCs were isolated and cultured, and then transplanted into an ectopic subcutaneous mouse model using a carrier treated either with or without rhBMP‐2. Comprehensive histologic, histometric and immunohistochemical analyses were performed after an 8‐wk healing period. The effects of rhBMP‐2 on the adipogenic and osteogenic/cementogenic differentiation of hPDLSCs were also evaluated. The effect of rhBMP‐2 on both soluble and insoluble collagen synthesis was analyzed, and the expression of mRNA and protein for collagen types I, II, III and V was assessed.</P><P><B>Results: </B> In the present study, rhBMP‐2 promoted both adipogenic and osteogenic/cementogenic differentiation of hPDLSCs <I>in vitro</I>, and the <I>in vivo</I> potential of hPDLSCs to form mineralized cementum and organized PDL tissue was down‐regulated following treatment with rhBMP‐2. Collagen synthesis, which plays a crucial role in the regeneration of cementum and the periodontal attachment, was significantly reduced, with associated modification of the relevant mRNA and protein expression profiles.</P><P><B>Conclusion: </B> In summary, the findings of the present study suggest that enhanced adipogenic differentiation and inhibition of collagen synthesis by hPDLSCs appear to be partly responsible for the minimal effect of rhBMP‐2 on cementum and PDL tissue regeneration by hPDLSCs.</P>

Glutathione S-transferase M1 and T1 polymorphisms: Susceptibility and outcomes in muscle invasive bladder cancer patients

Kang, H.w.,Song, P.H.,Ha, Y.S.,Kim, W.T.,Kim, Y.J.,Yun, S.J.,Lee, S.C.,Choi, Y.H.,Moon, S.K.,Kim, W.J. Pergamon Press 2013 European journal of cancer Vol.49 No.14

Background: We investigated whether genetic polymorphisms in the glutathione S transferase mu (GSTM1) and theta (GSTT1) genes modulated risk, disease progression and survival in primary muscle invasive bladder cancer (MIBC). Methods: GSTM1 and GSTT1 polymorphisms were analysed by multiplex polymerase chain reaction (PCR) using blood genomic DNA in 110 MIBC patients and 220 gender- and age-matched healthy controls. The influence of the genetic polymorphisms on patient survival was evaluated by Kaplan-Meier survival curves and Cox Proportional Hazard models. We also evaluated whether cigarette smoking and treatment modality modified the association between genotype and prognosis. Results: GSTM1-null individuals exhibited increased risk for MIBC and an association with cigarette smoking. GSTT1-null subjects showed significant disease progression and cancer-specific death. In the combined analysis, GSTT1-null genotype was an independent risk factor for disease progression and cancer specific death regardless of GSTM1 genotype. Significant differences in progression-free survival (PFS) and cancer-specific survival (CSS) were seen based on GSTT1 genotype. The survival impact of the GSTT1 genotype was only valid for smokers. The GSTT1-null genotype was an independent prognostic factor for shorter PFS in patients who received chemotherapy and those who did not undergo radical cystectomy. By multivariate Cox regression analysis, GSTT1-null genotype was a predictive factor for disease progression and cancer specific survival regardless of treatment modality. Conclusions: The GSTM1-null genotype plays an important role in genetic susceptibility to MIBC and the GSTT1-null genotype is associated with disease progression and shorter survival in MIBC.

Determination of optimal concentration of deglycyrrhizinated licorice root extract for preventing dental caries using a bacterial model system

Ahn, S.J.,Song, Y.D.,Mah, S.J.,Cho, E.J.,Kook, J.K. Association for Dental Sciences of the Republic of 2014 Journal of dental sciences Vol.9 No.3

Background/purpose: In prior studies, we induced the antimicrobial activity of deglycyrrhizinated licorice root extract (DG-LRE) by inhibiting the growth and biofilm formation of Streptococcus mutans UA159. Here, we used clinical strains of mutans streptococci (MS) collected from Koreans to determine the optimal concentration of DG-LRE for oral hygiene products to prevent dental caries. Materials and methods: Antimicrobial effects of DG-LRE against 14 clinical strains of MS were evaluated through the minimum inhibitory concentration, minimum bactericidal concentration, time-kill assay, and biofilm-forming assay. Results: Minimum inhibitory concentration and minimum bactericidal concentration values of DG-LRE against the clinical strains of MS ranged from 4 μg/mL to 8 μg/mL and from 8 μg/mL to 16 μg/mL, respectively. Time-kill assay demonstrated that the antimicrobial effects of DG-LRE primarily resulted from bactericidal activity. DG-LRE significantly decreased the biofilm formation of S. mutans ranging from 57.6% to 92.8% at 16 μg/mL. Conclusion: These findings reveal that a DG-LRE concentration of 16 μg/mL may be used to prevent dental caries in Koreans.

Emergence of Panton-Valentine leucocidin-positive ST8-methicillin-resistant Staphylococcus aureus (USA300 clone) in Korea causing healthcare-associated and hospital-acquired bacteraemia

Jung, J.,Song, E. H.,Park, S. Y.,Lee, S. R.,Park, S. J.,Sung, H.,Kim, M. N.,Kim, S. H.,Lee, S. O.,Choi, S. H. Springer Science + Business Media 2016 European journal of clinical microbiology & infect Vol.35 No.8

<P>Panton-Valentine leucocidin (PVL)-positive sequence type (ST)8-MRSA-SCCmec IVa (USA300) is the epidemic strain of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in North America. USA300 is extremely rare in South Korea, and PVL-negative ST72 SCCmec type IVc is the predominant CA-MRSA clone. In a multicentre, prospective cohort study of S. aureus bacteraemia, we identified PVL-positive ST8-MRSA isolates by performing multilocus sequence typing and PCR for PVL. We analyzed the clinical characteristics of patients with PVL-positive ST8-MRSA bacteraemia, and performed SCCmec, spa, and agr typing, PCR for arginine catabolic mobile element (ACME), virulence gene profiling, and pulsed-field gel electrophoresis (PFGE). Among a total of 818 MRSA isolates, we identified ten isolates of PVL-positive ST8-MRSA (USA300) (3 from Hospital D, 4 from Hospital G, and 3 from Hospital A), all of which involved exclusively healthcare-associated (5 isolates) and hospital-acquired bacteraemia (5 isolates). This strain accounted for 8 similar to 10 % of the hospital-acquired MRSA bacteraemia in Hospitals D and G. Bacteraemia of unknown origin was the most common type of infection followed by pneumonia. All the isolates were SCCmec type IVa, spa type t008, and agr group I. Eight of the isolates harboured ACME. In a PFGE analysis, four isolates were identical to the USA300 control strain, five differed by a single band, and the remaining one differed by two bands. All the isolates were pulsed-field type USA300. This is the first report of healthcare-associated and hospital-acquired bacteraemia caused by USA300 in South Korea. USA300 seems to be an emerging hospital clone in this country.</P>

The degree complexity of smooth surfaces of codimension 2

Ahn, J.,Kwak, S.,Song, Y. Academic Press 2012 Journal of symbolic computation Vol.47 No.5

For a given term order, the degree complexity of a projective scheme is defined by the maximal degree of the reduced Grobner basis of its defining saturated ideal in generic coordinates (Bayer and Mumford, 1993). It is well known that the degree complexity with respect to the graded reverse lexicographic order is equal to the Castelnuovo-Mumford regularity (Bayer and Stillman, 1987). However, much less is known if one uses the graded lexicographic order (Ahn, 2008; Conca and Sidman, 2005). In this paper, we study the degree complexity of a smooth irreducible surface in P<SUP>4</SUP> with respect to the graded lexicographic order and its geometric meaning. As in the case of a smooth curve (Ahn, 2008), we expect that this complexity is closely related to the invariants of the double curve of a surface under a generic projection. As results, we prove that except in a few cases, the degree complexity of a smooth surface S of degree d with h<SUP>0</SUP>(I<SUB>S</SUB>(2))<>0 in P<SUP>4</SUP> is given by 2+(degY<SUB>1</SUB>(S)-12)-g(Y<SUB>1</SUB>(S)), where Y<SUB>1</SUB>(S) is a double curve of degree (d-12)-g(S@?H) under a generic projection of S. In particular, this complexity is actually obtained at the monomial x<SUB>0</SUB>x<SUB>1</SUB>x<SUB>3</SUB><SUP>(degY<SUB>1</SUB>(S)-12)-g(Y<SUB>1</SUB>(S))</SUP> where k[x<SUB>0</SUB>,x<SUB>1</SUB>,x<SUB>2</SUB>,x<SUB>3</SUB>,x<SUB>4</SUB>] is a polynomial ring defining P<SUP>4</SUP>. Exceptional cases are a rational normal scroll, a complete intersection surface of (2,2)-type, or a Castelnuovo surface of degree 5 in P<SUP>4</SUP> whose degree complexities are in fact equal to their degrees. This complexity can also be expressed in terms of degrees of defining equations of I<SUB>S</SUB> in the same manner as the result of A. Conca and J. Sidman (Conca and Sidman, 2005). We also provide some illuminating examples of our results via calculations done withMacaulay 2 (Grayson and Stillman, 1997).