In vitro Antioxidant Activity of Ethanolic Extract of Chlorophytum borivilianum

R. Govindarajan1,,N. Sreevidya1,,M. Vijayakumar1,,M. Thakur2,,V.K. Dixit2,,S. Mehrotra*1,,P. Pushpangadan1 한국생약학회 2005 Natural Product Sciences Vol.11 No.3

Chlorophytum borivilianum Baker (Antharicaceae) comonly referred as ‘Safed Musli’ has beenwidely used in the Indian traditional systems of medicine to treat various diseases like rheumatism apart fromaphordisiac properties. C. borivilianum was screened for the first time to determine its antioxidant activity,isolation of the sapogenins and standardization of the isolated sapogenin fraction using HPTLC. Potent antioxidantactivity of ethanolic extract was found by their ability to scavenge DPPH (84.51%), hydroxyl radical (48.95 %),feryl bi-pyridyl complex (84.53%) along with the inhibition of lipid peroxidation (67.17%) at 100g/mlconcentration. The ethanolic extract also exhibited significant inhibition of superoxide anion radical generated byplant in the traditional system especially its use as a Rasayana drug.

Experimental estimate of Nγ values and corresponding settlements for square footings on finite layer of sand

Dixit, Manish S.,Patil, Kailas A. Techno-Press 2013 Geomechanics & engineering Vol.5 No.4

Any structure constructed on the earth is supported by the underlying soil. Foundation is an interfacing element between superstructure and the underlying soil that transmits the loads supported by the foundation including its self weight. Foundation design requires evaluation of safe bearing capacity along with both immediate and long term settlements. Weak and compressible soils are subjected to problems related to bearing capacity and settlement. The conventional method of design of footing requires sufficient safety against failure and the settlement must be kept within the allowable limit. These requirements are dependent on the bearing capacity of soil. Thus, the estimation of load carrying capacity of footing is the most important step in the design of foundation. A number of theoretical approaches, in-situ tests and laboratory model tests are available to find out the bearing capacity of footings. The reliability of any theory can be demonstrated by comparing it with the experimental results. Results from laboratory model tests on square footings resting on sand are presented in this paper. The variation of bearing capacity of sand below a model plate footing of square shape with variation in size, depth and the effect of permissible settlement are evaluated. A steel tank of size $900mm{\times}1200mm{\times}1000mm$ is used for conducting model tests. Bearing capacity factor $N_{\gamma}$ is evaluated and is compared with Terzaghi, Meyerhof, Hansen and Vesic's $N_{\gamma}$ values. From the experimental investigations it is found that, as the depth of sand cushion below the footing ($D_{sc}$) increases, ultimate bearing capacity and settlement values show an increasing trend up to a certain depth of sand cushion.

Penicillin G as a novel chiral selector in capillary electrophoresis

Dixit, S.,Park, J.H. Elsevier 2014 Journal of chromatography A Vol.1326 No.-

The penicillin sub-class of β-lactam antibiotics has not been examined for its enantiodiscriminating abilities in capillary electrophoresis (CE) until date. The present work was therefore designed to evaluate penicillin G potassium salt (PenG) as an ion-pair chiral selector (CS) using CE for its several attributes, namely, high solubility in water and lower alcohols, structure allowing multiple interactions with analytes and cost-effectiveness. Systematic experiments were performed to investigate the effect of composition of background electrolyte, applied voltage and capillary temperature on chiral separation. Baseline resolutions of enantiomers of five basic chiral drugs (namely, darifenacin, citalopram, sertraline, propranolol and metoprolol) were attained using a background electrolyte composed of water:methanol (90:10, v/v) and consisting of 10.7 or 16.1mM CS at 20<SUP>o</SUP>C using an applied voltage of 5kV.

A comparative study of thermal and hydraulic autofrettage

S. M. Kamal,U. S. Dixit 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.6

Thermal autofrettage is a potential process to generate beneficial compressive residual stresses at and around the inner wall of a thickwalled cylinder for increasing its pressure carrying capacity. Due to its simplicity and inexpensive arrangement, it can compete with the conventional hydraulic autofrettage process. In this work, a comparative study of the thermal autofrettage and the hydraulic autofrettage is carried out. As the thermal autofrettage does not require hydraulic power pack, the process is more economical than the hydraulic autofrettage. The thermal autofrettage process is also studied for the thick-walled cylindrical vessels subjected to high thermal gradient with or without pressure and is compared with the hydraulic autofrettage. Comparison shows that for cylinders subjected to high thermal gradient without pressure, the thermal autofrettage is superior to the hydraulic autofrettage.

Genetic Structure and Differentiation of Three Indian Goat Breeds

Dixit, S.P.,Verma, N.K.,Aggarwal, R.A.K.,Kumar, Sandeep,Chander, Ramesh,Vyas, M.K.,Singh, K.P. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.9

Gene flow, genetic structure and differentiation of Kutchi, Mehsana and Sirohi breeds of goat from North-Western India were evaluated based on 25 microsatellite markers so as to support breed conservation and improvement decisions. The microsatellite genotyping was carried out using an automated DNA sequencer. The gene diversity across the studied loci for the Kutchi breed varied from 0.57 (ILST 065) to 0.93 (OarFCB 304, OMHC 1, ILSTS 058) with an overall mean of 0.79${\pm}$0.02. The corresponding values for Mehsana and Sirohi breeds were 0.16 (ILST 008) to 0.93 (OMHC 1, ILSTS 058) with an average of 0.76${\pm}$0.04, and 0.50 (ILSTS 029) to 0.94 (ILSTS 058) with an average of 0.78${\pm}$0.02, respectively. The Mehsana breed had lowest gene diversity among the 3 breeds studied. All the populations showed an overall significant heterozygote deficit ($F_{is}$). The Fis values were 0.26, 0.14 and 0.36 for Kutchi, Mehsana and Sirohi goat breeds, respectively. Kutchi and Mehsana were more differentiated (16%) followed by Mehsana and Sirohi (13%).The measures of standard genetic distance between pairs of breeds indicated that the lowest genetic distance was between Kutchi and Sirohi breeds (0.73) and the largest genetic distance was between Mehsana and Kutchi (1.0) followed by Sirohi and Mehsana (0.75) breeds. Mehsana and Kutchi are distinct breeds and this was revealed by the estimated genetic distance between them. All measures of genetic variation revealed substantial genetic variation in each of the populations studied, thereby showing good scope for their further improvement.

Application of rifampicin as a chiral selector for enantioresolution of basic drugs using capillary electrophoresis

Dixit, S.,Park, J.H. Elsevier 2016 Journal of chromatography Vol.1453 No.-

<P>Rifampicin, a member of rifamycin sub-class of antibiotics which belongs to the naphthalenic ansamycin class of antibiotics, has a characteristic ansa structure, i.e., a ring structure or chromophore spanned by an aliphatic chain. The present work was designed to evaluate its potential as a chiral selector (CS) as its structure consisting of nine stereogenic centers, an aromatic moiety and several functional groups (i.e., one imine, one amide, one acetoxy residue, two aliphatic hydroxyl and three phenolic hydroxyl groups) was expected to instigate multiple enantioselective interactions, namely, hydrogen bonding and inclusion complexation with chiral analytes, and therefore resulting in efficient enantioseparations. Systematic experiments were performed to investigate the effects of concentration of CS, composition of background electrolyte (BGE) and applied voltage on chiral separation. Enantiomers of propranolol and metoprolol were baseline resolved using a BGE consisting of 20 mM CS and 50/50 (v/v) iso-propanol/phosphate buffer (100 mM, pH TO) whereas for enantiomers of sertraline, a BGE consisting of 23 mM CS and 40/60 (v/v) iso-propanol/phosphate buffer (100 mM, pH 7.0) resulted in baseline resolutions. (C) 2016 Elsevier B.V. All rights reserved.</P>

Carbamoylated azithromycin incorporated zirconia hybrid monolith for enantioseparation of acidic chiral drugs using non-aqueous capillary electrochromatography

Dixit, S.,Lee, I.S.,Park, J.H. Elsevier 2017 Journal of chromatography Vol.1507 No.-

<P>Carbamoylated derivatives of two antibiotics, namely, clindamycin phosphate (CLIP) and erythromycin (ERY) were successfully employed as co-precursors, in combination of zirconium tetrabutoxide as a precursor, to prepare chiral organic-zirconia hybrid monoliths (i.e., CLIP-ZHMs and ERY-ZHMs, respectively) via a single-step in-situ sol-gel approach in our previous works. Their superiority over chiral organiczirconia/silica monoliths, prepared by post-modification approach, in terms of better enantioresolution and enhanced stability inspired us to prepare ZHM5 based on an another antibiotic, azithromycin (i.e., AZI-ZHMs). Monolithic columns were employed for capillary electrochromatographic enantioseparation of acidic chiral drugs in mobile phases consisting of acetonitrile (ACN) and methanol (MeOH) as organic modifiers, and acetic acid (AcOH) and triethylamine (TEA) as electrolytes. The effects of composition of mobile phase and applied voltage on chiral separation were investigated by using ketoprofen as a representative analyte. Baseline resolutions were obtained for six acidic drugs in mobile phase consisting of 80/20 (v/v) ACN/MeOH with 300 mM AcOH and 10 mM TEA at a 10 kV applied voltage and 25 degrees C capillary temperature. The relative standard deviations for resolution values regarding column to column and batch to batch repeatability were less than 2.5% (for n = 3) under optimized conditions, indicating satisfactory stability of the columns and reproducibility of the column preparation process. (C) 2017 Elsevier B.V. All rights reserved.</P>

Genetic Diversity in Attappady Breed of Indian Goat as Analyzed with Microsatellite Markers

D. Kumar,S. P. S. Ahlawat,S. Kumar,Y. Kumar,R. A. K. Aggarwal,S. P. Dixit,N. K. Verma,S. Mathew 한국유전학회 2006 Genes & Genomics Vol.28 No.3

Genetic Analysis of the Sirohi Breed of Indian Goat (Capra hircus)

N. K. Verma,S. P. Dixit,R. A. K. Aggarwal,R. Chander S. Kumar,S. P. S. Ahlawat 한국유전학회 2007 Genes & Genomics Vol.29 No.2

Genetic Variability and Bottleneck Analysis of Jakhrana Goats by Microsatellite DNA Fingerprinting

Dinesh Kumar,A. K. Pandey,S. P. S. Ahlawat,Geetu Malik,Rekha Sharma,Digpal Singh Gour,S. P. Dixit,N. K. Verma,R. A. K. Aggarwal,Neelam Gupta,B. N. Shahi,S. C. Gupta 한국유전학회 2005 Genes & Genomics Vol.27 No.3